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The map label for this gene is aprN [H]

Identifier: 118479933

GI number: 118479933

Start: 4595665

End: 4596807

Strand: Direct

Name: aprN [H]

Synonym: BALH_4378

Alternate gene names: 118479933

Gene position: 4595665-4596807 (Clockwise)

Preceding gene: 118479932

Following gene: 118479934

Centisome position: 87.42

GC content: 33.33

Gene sequence:

>1143_bases
ATGAAAAAAATAATCATTCGTAATTTACCCTTATTTGTCTTTACTGTTTTCTCTTTATTAACCTTCTTTTTTAGCGAGTT
AAATGAAAAAAATGCATTTGCTGCTGAACATGATATAAAAAAAGACTACTTAATTATGTTTAATAACACAGTTGACGAAC
AACTAATCGTTGAACATGGTGGTGTTATTAAAGAAACCTTTGAAAATTTACCTATTATTAAAGCTGAATTCTCCTATAAT
CCTGAAAAAGTTTTAAAACATAATAGTAATGTTAGTGAAGTGGAAGTAGATCAAACTATTAAAGCCCTTGGGGATAGTAA
TACTCAAACTAATCCCTCAATGACACAATCAAAATCCATCAACACTATTCCTTTCACTGGAAAAGGGGTATCTGTTGCTA
TATTGGATTCGGGAATTGATACAGAACATACAGATTTAAAAATAAAAGACGGTATTTCATTTGTAGAGAATCACCCAAGT
TTTGATGATGATAACGGTCACGGGACACATTTAGCAGGAATTGTCGCAGCACAAGATAATGAATTAGGAATGACAGGTAT
AGCACCTAATGTAGATTTATATGCCGTAAAAGTATTAGATAAATATACAAATGGAAAATACAGTACAGTTGTAAAGGGAA
TAGACTGGGCCATTGAACATAATGTAAATATTGTTTTAATGAGCTTAGGAGGAAAGAAAGAGTCAGCATTTTTTAAAGAA
GCTATGGACAAGGCTTATCAAAAGGGTATCTTATTAATTGCCTCCGCTGGTAATGAGGGGTATAAGGACGGAGATACAAT
TTCTTATCCTGCAAAATTCCAATCAGTAGTTGCTGTCGGAGCTTTAGATAAAAATGATGCACGCGGTTTTCTTTCAAGTA
AAGGTGATGAACTTGAATTAATGGCACCTGGTGTTGATATTTTAAGTACATGGAAAGATGGAAATTACAGATTAGATAGC
GGAACATCTATGGCAGCAGCCCACGTCGTGGGCGTTGCTTCATTAATATTTGAAAAGAACCCTTATTTATCAAATAAACA
AGTTAGAGAGATTATGAATAAAGCGGCTATTCCTCTTGGTAATGCTTTTGAATATGGTAATGGTAAGATAAATGTAGACT
ACGCTCTAAATATGGCGGATTAA

Upstream 100 bases:

>100_bases
CAAGGGATATATACAGATATTTGTGATCATTTAATTTCAGCATCACAAAAATAAGACGCACATTGTGCCATATTTTTACG
TTTGAAAGGACTAAAATTTA

Downstream 100 bases:

>100_bases
ACATCATGTTAAAAGGTGCACTTAAATATGAGTGCACCTTTTAACATGATTTCACACAAATATTATTATGCTAATCTAAA
GTTTCCGTATTTACATTTTT

Product: alkaline serine protease

Products: NA

Alternate protein names: Nattokinase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKKIIIRNLPLFVFTVFSLLTFFFSELNEKNAFAAEHDIKKDYLIMFNNTVDEQLIVEHGGVIKETFENLPIIKAEFSYN
PEKVLKHNSNVSEVEVDQTIKALGDSNTQTNPSMTQSKSINTIPFTGKGVSVAILDSGIDTEHTDLKIKDGISFVENHPS
FDDDNGHGTHLAGIVAAQDNELGMTGIAPNVDLYAVKVLDKYTNGKYSTVVKGIDWAIEHNVNIVLMSLGGKKESAFFKE
AMDKAYQKGILLIASAGNEGYKDGDTISYPAKFQSVVAVGALDKNDARGFLSSKGDELELMAPGVDILSTWKDGNYRLDS
GTSMAAAHVVGVASLIFEKNPYLSNKQVREIMNKAAIPLGNAFEYGNGKINVDYALNMAD

Sequences:

>Translated_380_residues
MKKIIIRNLPLFVFTVFSLLTFFFSELNEKNAFAAEHDIKKDYLIMFNNTVDEQLIVEHGGVIKETFENLPIIKAEFSYN
PEKVLKHNSNVSEVEVDQTIKALGDSNTQTNPSMTQSKSINTIPFTGKGVSVAILDSGIDTEHTDLKIKDGISFVENHPS
FDDDNGHGTHLAGIVAAQDNELGMTGIAPNVDLYAVKVLDKYTNGKYSTVVKGIDWAIEHNVNIVLMSLGGKKESAFFKE
AMDKAYQKGILLIASAGNEGYKDGDTISYPAKFQSVVAVGALDKNDARGFLSSKGDELELMAPGVDILSTWKDGNYRLDS
GTSMAAAHVVGVASLIFEKNPYLSNKQVREIMNKAAIPLGNAFEYGNGKINVDYALNMAD
>Mature_380_residues
MKKIIIRNLPLFVFTVFSLLTFFFSELNEKNAFAAEHDIKKDYLIMFNNTVDEQLIVEHGGVIKETFENLPIIKAEFSYN
PEKVLKHNSNVSEVEVDQTIKALGDSNTQTNPSMTQSKSINTIPFTGKGVSVAILDSGIDTEHTDLKIKDGISFVENHPS
FDDDNGHGTHLAGIVAAQDNELGMTGIAPNVDLYAVKVLDKYTNGKYSTVVKGIDWAIEHNVNIVLMSLGGKKESAFFKE
AMDKAYQKGILLIASAGNEGYKDGDTISYPAKFQSVVAVGALDKNDARGFLSSKGDELELMAPGVDILSTWKDGNYRLDS
GTSMAAAHVVGVASLIFEKNPYLSNKQVREIMNKAAIPLGNAFEYGNGKINVDYALNMAD

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=276, Percent_Identity=27.8985507246377, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI31317307, Length=265, Percent_Identity=27.9245283018868, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71983555, Length=274, Percent_Identity=27.007299270073, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25141268, Length=274, Percent_Identity=27.007299270073, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324576, Length=231, Percent_Identity=33.7662337662338, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6320775, Length=328, Percent_Identity=29.2682926829268, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319893, Length=261, Percent_Identity=31.0344827586207, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45550681, Length=269, Percent_Identity=32.3420074349442, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 41548; Mature: 41548

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIIRNLPLFVFTVFSLLTFFFSELNEKNAFAAEHDIKKDYLIMFNNTVDEQLIVEHG
CCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEECCCCCHHHHHHCC
GVIKETFENLPIIKAEFSYNPEKVLKHNSNVSEVEVDQTIKALGDSNTQTNPSMTQSKSI
CHHHHHHHCCCEEEEECCCCHHHHHHCCCCCCEEHHHHHHHHHCCCCCCCCCCCHHCCCC
NTIPFTGKGVSVAILDSGIDTEHTDLKIKDGISFVENHPSFDDDNGHGTHLAGIVAAQDN
CCCCCCCCCEEEEEEECCCCCCCCCEEECCCCHHHHCCCCCCCCCCCCCEEEEEEEECCC
ELGMTGIAPNVDLYAVKVLDKYTNGKYSTVVKGIDWAIEHNVNIVLMSLGGKKESAFFKE
CCCCCCCCCCCCEEEEEEEHHHCCCCCEEEEECCCEEEECCCEEEEEECCCCCHHHHHHH
AMDKAYQKGILLIASAGNEGYKDGDTISYPAKFQSVVAVGALDKNDARGFLSSKGDELEL
HHHHHHHCCEEEEEECCCCCCCCCCCEECCHHHHHEEEEECCCCCCCCHHHCCCCCEEEE
MAPGVDILSTWKDGNYRLDSGTSMAAAHVVGVASLIFEKNPYLSNKQVREIMNKAAIPLG
EECCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCC
NAFEYGNGKINVDYALNMAD
CCEECCCCEEEEEEEEEECC
>Mature Secondary Structure
MKKIIIRNLPLFVFTVFSLLTFFFSELNEKNAFAAEHDIKKDYLIMFNNTVDEQLIVEHG
CCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEECCCCCHHHHHHCC
GVIKETFENLPIIKAEFSYNPEKVLKHNSNVSEVEVDQTIKALGDSNTQTNPSMTQSKSI
CHHHHHHHCCCEEEEECCCCHHHHHHCCCCCCEEHHHHHHHHHCCCCCCCCCCCHHCCCC
NTIPFTGKGVSVAILDSGIDTEHTDLKIKDGISFVENHPSFDDDNGHGTHLAGIVAAQDN
CCCCCCCCCEEEEEEECCCCCCCCCEEECCCCHHHHCCCCCCCCCCCCCEEEEEEEECCC
ELGMTGIAPNVDLYAVKVLDKYTNGKYSTVVKGIDWAIEHNVNIVLMSLGGKKESAFFKE
CCCCCCCCCCCCEEEEEEEHHHCCCCCEEEEECCCEEEECCCEEEEEECCCCCHHHHHHH
AMDKAYQKGILLIASAGNEGYKDGDTISYPAKFQSVVAVGALDKNDARGFLSSKGDELEL
HHHHHHHCCEEEEEECCCCCCCCCCCEECCHHHHHEEEEECCCCCCCCHHHCCCCCEEEE
MAPGVDILSTWKDGNYRLDSGTSMAAAHVVGVASLIFEKNPYLSNKQVREIMNKAAIPLG
EECCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCC
NAFEYGNGKINVDYALNMAD
CCEECCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1369081 [H]