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The map label for this gene is trmB [H]

Identifier: 118479836

GI number: 118479836

Start: 4496579

End: 4497232

Strand: Reverse

Name: trmB [H]

Synonym: BALH_4273

Alternate gene names: 118479836

Gene position: 4497232-4496579 (Counterclockwise)

Preceding gene: 118479838

Following gene: 118479835

Centisome position: 85.55

GC content: 34.86

Gene sequence:

>654_bases
ATGCGTTTAAGACATAAACCATATGCAATGGATCGAATTAATGAGTATTCACATTTCGTAATCGGAAACCCAGAGGAGCG
CGCTGGTAACTGGAAAGAAGTATTTGGAAATGAACAGCCAATTCACATTGAAGTAGGTACAGGTCGTGGCCGTTTTATGT
ATGATATGGCAAAAGCAAATCCTCATATTAATTATATTGGAATTGAAAAATTCACAAGTGTTGTTGTAGATGCACTGGAT
AAATTAATTGAAGAAGAATTACCGAACTTAAAGTTAATTAATAAAGATGCTGAAGATTTAACAGTTTTCTTTGCAAAAGG
TGAGATTGATCGCGTGTATTTAAACTTCTCAGATCCATGGCCAAAGAAACGTCATACGAAGCGTCGTTTAACGTATAAAA
CGTTTTTACGCAATTATGAAGAAGTGTTAGTAGAAGGCGGAGAAATTCATTTCAAAACAGATAACCAAGGTTTATTTGAA
TATTCTCTTATGAGTATGGCTGAGTATGGTATGTTACTAACTTACTTAAGCTTAGATCTTCATAATAGCGACTTTGAAGG
TAACATTATGACTGAGTACGAAGAGAAATTCTCTAGTAAAGGTCATCGCATTTACCGAGTAGAAGCAAAATATCGTACAG
AGCCTATGCAGTAA

Upstream 100 bases:

>100_bases
GAGTGCTCGTACGTACTTGGTTTTTTGTTTGGTAGATTGATTAAAACAGAAAGAAGGAATTATTCCTTTAAAATAGCTAT
GTAAAGAGAGGGAAACATCT

Downstream 100 bases:

>100_bases
TGCATAGGTTTTTTGTGTCTAAAATAATCAGAATTTTATGTTAGTATTGTAAGAAAAGGGGGATGAAAAATGGAACAGTT
ACAAATTGGAGATATAAAAG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MRLRHKPYAMDRINEYSHFVIGNPEERAGNWKEVFGNEQPIHIEVGTGRGRFMYDMAKANPHINYIGIEKFTSVVVDALD
KLIEEELPNLKLINKDAEDLTVFFAKGEIDRVYLNFSDPWPKKRHTKRRLTYKTFLRNYEEVLVEGGEIHFKTDNQGLFE
YSLMSMAEYGMLLTYLSLDLHNSDFEGNIMTEYEEKFSSKGHRIYRVEAKYRTEPMQ

Sequences:

>Translated_217_residues
MRLRHKPYAMDRINEYSHFVIGNPEERAGNWKEVFGNEQPIHIEVGTGRGRFMYDMAKANPHINYIGIEKFTSVVVDALD
KLIEEELPNLKLINKDAEDLTVFFAKGEIDRVYLNFSDPWPKKRHTKRRLTYKTFLRNYEEVLVEGGEIHFKTDNQGLFE
YSLMSMAEYGMLLTYLSLDLHNSDFEGNIMTEYEEKFSSKGHRIYRVEAKYRTEPMQ
>Mature_217_residues
MRLRHKPYAMDRINEYSHFVIGNPEERAGNWKEVFGNEQPIHIEVGTGRGRFMYDMAKANPHINYIGIEKFTSVVVDALD
KLIEEELPNLKLINKDAEDLTVFFAKGEIDRVYLNFSDPWPKKRHTKRRLTYKTFLRNYEEVLVEGGEIHFKTDNQGLFE
YSLMSMAEYGMLLTYLSLDLHNSDFEGNIMTEYEEKFSSKGHRIYRVEAKYRTEPMQ

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=176, Percent_Identity=32.3863636363636, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 25634; Mature: 25634

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRHKPYAMDRINEYSHFVIGNPEERAGNWKEVFGNEQPIHIEVGTGRGRFMYDMAKAN
CCCCCCCCHHHHHCCCCEEEECCCHHHCCCHHHHCCCCCCEEEEEECCCCCEEEEEHHCC
PHINYIGIEKFTSVVVDALDKLIEEELPNLKLINKDAEDLTVFFAKGEIDRVYLNFSDPW
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCEEEEEEECCCCC
PKKRHTKRRLTYKTFLRNYEEVLVEGGEIHFKTDNQGLFEYSLMSMAEYGMLLTYLSLDL
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEC
HNSDFEGNIMTEYEEKFSSKGHRIYRVEAKYRTEPMQ
CCCCCCCCEEHHHHHHHCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MRLRHKPYAMDRINEYSHFVIGNPEERAGNWKEVFGNEQPIHIEVGTGRGRFMYDMAKAN
CCCCCCCCHHHHHCCCCEEEECCCHHHCCCHHHHCCCCCCEEEEEECCCCCEEEEEHHCC
PHINYIGIEKFTSVVVDALDKLIEEELPNLKLINKDAEDLTVFFAKGEIDRVYLNFSDPW
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCEEEEEEECCCCC
PKKRHTKRRLTYKTFLRNYEEVLVEGGEIHFKTDNQGLFEYSLMSMAEYGMLLTYLSLDL
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEC
HNSDFEGNIMTEYEEKFSSKGHRIYRVEAKYRTEPMQ
CCCCCCCCEEHHHHHHHCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA