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The map label for this gene is acsA [H]

Identifier: 118479786

GI number: 118479786

Start: 4446989

End: 4448581

Strand: Reverse

Name: acsA [H]

Synonym: BALH_4223

Alternate gene names: 118479786

Gene position: 4448581-4446989 (Counterclockwise)

Preceding gene: 118479787

Following gene: 118479785

Centisome position: 84.62

GC content: 39.8

Gene sequence:

>1593_bases
ATGTTTATGAAGAGAGAAGAATTGTTGGCGCCACCGTCTTATAATTTAGTTTCTGAAATAGAGAAATATACAGGTGATAA
AGGAAAATTAGCTTTAATTTGGCAAGATGATAAAGGGAATCGACGAGAAGTTACATACGCTGAGTTAATGAAAGGTGCAA
ATAAAATTGGAAACGCTTTTATAAAGAGTGGTCTGCAAAAAGGGGACAAGCTTCTCATTATGATGCCACGCTTAATTGAA
GCGTACATGACGTATATCGCTGCGATTAAAGCAGGATTTGTCGTAATTCCGAGTTCGGAAATGTTGCGCAAAAAGGATAT
CGAATATCGCATTGGCCACGGGGAAGTAAAAGCAATTGTAAGTTATGAGCCGTACATTGGACAGTTTGATGATATAGAAG
CGATGGAATCTCTTCAAAAATTCGTTCTGAGCGAGCAGTCAGTAGATGGATGGATTAATTTAAAAACAGCGTTAGAAACA
GAGAGTGACATGTTAGAAATGGCGAAGACAGATAAAGAAGATATGGTCTTTTTATCTTACACGTCAGGAACGACAGGAAA
TCCAAAAGGTGTTGTTCATACGCATGCGTGGGCATACGCTCATTTACGTACAAGCGCTCCAAATTGGCTTGGAATTGAAG
AGAATGATGTTGTATGGGCAACAGCTAGTCCAGGCTGGCAGAAGTGGATTTGGAGCCCATTTTTAGCAACTCTCGGATCA
GGAGCGACAGGATTTGTATACCACGGTAAATTTGAACCGAAAACGTATTTAAATTTATTAGACGATAATAAGGTGAATGT
GCTTTGTTGTACGCCGACTGAGTATAGATTAATGGCAAAAGTAGAAAATTTAAGTGAGTACAATTTAGAAGCGTTACATA
GCGCTGTATCCGCAGGTGAGCCGTTAAATAGAGAAGTCATTGAAACGTTCCAAAGGCACTTTCATATTACAGTGAGAGAC
GGCTATGGACAAACGGAAAATACATTGCTCGTTGGTGTAATGAAAGGGATGGATATTAGACCAGGATCAATGGGGAAACC
AACGCCAGGCAACCACGTTGATATTGTAAATGAGGAAGGTATTCCGGTACAGGTAGGAGAAGTTGGTGATATCGCAGTTC
ACATTGAAACGCCAGCCCTCTTTAAACAATACTATAAAGACGATGAGCGCACAGCGATGCAATTCCGAGGTGATTATTAT
ATTACCGGTGATAAAGCGAAGAAAGATGAAGACGGCTATTTCTGGTTTGAAGGGCGCGGTGATGATATTATTATTAGCTC
TGGCTATACAATCGGGCCGTTTGAAGTAGAAGATGCGCTCGTAAAGCACCCTTATGTAAGAGAATGTGCAGTTGTTGCAA
GCCCTGATGAAATTCGCGGAAGTGTCGTGAAGGCATTTATCGTATTAAGAGAGAATATAGAAAAGAACGAAGAAACATTA
ATTCCAATACTCCAACAACACGTCAAAGAACTTACTGCACCATATAAATACCCTCGTAAAATTGAATTTGTAGATGAACT
ACCAAAAACGATTTCTGGAAAAATCCGCCGTATTGAACTTAGAAAACAAGAGATGGAGATTCCGTCAAAATAA

Upstream 100 bases:

>100_bases
TTACCAATTCGACAGAACTGAAAAATTTGTCAAAAGAAGAGGGTTCAAAATAGACAGAAAATTCTTTATTATATAAGGGA
GGCATAAAGAAAAAAGGAGG

Downstream 100 bases:

>100_bases
ATTTGAAAACAGTGAAGGAAATAGTTGAATGAAATGGCAAACAATGATAATATAAAAACGAACACAAGTTCGATTTTATA
AATTGAAAAAAGAGGTGTCC

Product: acetate--CoA ligase

Products: AMP; diphosphate; acetyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MFMKREELLAPPSYNLVSEIEKYTGDKGKLALIWQDDKGNRREVTYAELMKGANKIGNAFIKSGLQKGDKLLIMMPRLIE
AYMTYIAAIKAGFVVIPSSEMLRKKDIEYRIGHGEVKAIVSYEPYIGQFDDIEAMESLQKFVLSEQSVDGWINLKTALET
ESDMLEMAKTDKEDMVFLSYTSGTTGNPKGVVHTHAWAYAHLRTSAPNWLGIEENDVVWATASPGWQKWIWSPFLATLGS
GATGFVYHGKFEPKTYLNLLDDNKVNVLCCTPTEYRLMAKVENLSEYNLEALHSAVSAGEPLNREVIETFQRHFHITVRD
GYGQTENTLLVGVMKGMDIRPGSMGKPTPGNHVDIVNEEGIPVQVGEVGDIAVHIETPALFKQYYKDDERTAMQFRGDYY
ITGDKAKKDEDGYFWFEGRGDDIIISSGYTIGPFEVEDALVKHPYVRECAVVASPDEIRGSVVKAFIVLRENIEKNEETL
IPILQQHVKELTAPYKYPRKIEFVDELPKTISGKIRRIELRKQEMEIPSK

Sequences:

>Translated_530_residues
MFMKREELLAPPSYNLVSEIEKYTGDKGKLALIWQDDKGNRREVTYAELMKGANKIGNAFIKSGLQKGDKLLIMMPRLIE
AYMTYIAAIKAGFVVIPSSEMLRKKDIEYRIGHGEVKAIVSYEPYIGQFDDIEAMESLQKFVLSEQSVDGWINLKTALET
ESDMLEMAKTDKEDMVFLSYTSGTTGNPKGVVHTHAWAYAHLRTSAPNWLGIEENDVVWATASPGWQKWIWSPFLATLGS
GATGFVYHGKFEPKTYLNLLDDNKVNVLCCTPTEYRLMAKVENLSEYNLEALHSAVSAGEPLNREVIETFQRHFHITVRD
GYGQTENTLLVGVMKGMDIRPGSMGKPTPGNHVDIVNEEGIPVQVGEVGDIAVHIETPALFKQYYKDDERTAMQFRGDYY
ITGDKAKKDEDGYFWFEGRGDDIIISSGYTIGPFEVEDALVKHPYVRECAVVASPDEIRGSVVKAFIVLRENIEKNEETL
IPILQQHVKELTAPYKYPRKIEFVDELPKTISGKIRRIELRKQEMEIPSK
>Mature_530_residues
MFMKREELLAPPSYNLVSEIEKYTGDKGKLALIWQDDKGNRREVTYAELMKGANKIGNAFIKSGLQKGDKLLIMMPRLIE
AYMTYIAAIKAGFVVIPSSEMLRKKDIEYRIGHGEVKAIVSYEPYIGQFDDIEAMESLQKFVLSEQSVDGWINLKTALET
ESDMLEMAKTDKEDMVFLSYTSGTTGNPKGVVHTHAWAYAHLRTSAPNWLGIEENDVVWATASPGWQKWIWSPFLATLGS
GATGFVYHGKFEPKTYLNLLDDNKVNVLCCTPTEYRLMAKVENLSEYNLEALHSAVSAGEPLNREVIETFQRHFHITVRD
GYGQTENTLLVGVMKGMDIRPGSMGKPTPGNHVDIVNEEGIPVQVGEVGDIAVHIETPALFKQYYKDDERTAMQFRGDYY
ITGDKAKKDEDGYFWFEGRGDDIIISSGYTIGPFEVEDALVKHPYVRECAVVASPDEIRGSVVKAFIVLRENIEKNEETL
IPILQQHVKELTAPYKYPRKIEFVDELPKTISGKIRRIELRKQEMEIPSK

Specific function: Enables The Cell To Use Acetate During Aerobic Growth To Generate Energy Via The Tca Cycle, And Biosynthetic Compounds Via The Glyoxylate Shunt. Acetylates Chey, The Response Regulator Involved In Flagellar Movement And Chemotaxis. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=538, Percent_Identity=40.1486988847584, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI38505220, Length=502, Percent_Identity=41.0358565737052, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI122937307, Length=529, Percent_Identity=38.1852551984877, Blast_Score=358, Evalue=9e-99,
Organism=Homo sapiens, GI157311624, Length=535, Percent_Identity=36.6355140186916, Blast_Score=348, Evalue=7e-96,
Organism=Homo sapiens, GI157311622, Length=535, Percent_Identity=36.6355140186916, Blast_Score=348, Evalue=7e-96,
Organism=Homo sapiens, GI58082049, Length=535, Percent_Identity=36.6355140186916, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI115511026, Length=530, Percent_Identity=36.2264150943396, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI42544134, Length=368, Percent_Identity=32.3369565217391, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI195947378, Length=421, Percent_Identity=28.978622327791, Blast_Score=193, Evalue=4e-49,
Organism=Homo sapiens, GI13375727, Length=552, Percent_Identity=28.4420289855072, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI28416953, Length=532, Percent_Identity=28.7593984962406, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI253970489, Length=577, Percent_Identity=27.7296360485269, Blast_Score=175, Evalue=9e-44,
Organism=Homo sapiens, GI8923896, Length=567, Percent_Identity=28.042328042328, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI156151445, Length=525, Percent_Identity=26.2857142857143, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI187761345, Length=528, Percent_Identity=25.7575757575758, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI187761343, Length=528, Percent_Identity=25.7575757575758, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI31982927, Length=524, Percent_Identity=21.1832061068702, Blast_Score=80, Evalue=7e-15,
Organism=Escherichia coli, GI1790505, Length=529, Percent_Identity=28.1663516068053, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1788107, Length=491, Percent_Identity=25.4582484725051, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI221142682, Length=504, Percent_Identity=24.6031746031746, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1786529, Length=535, Percent_Identity=26.5420560747664, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI145693145, Length=500, Percent_Identity=26.2, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1786810, Length=503, Percent_Identity=24.8508946322068, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789201, Length=369, Percent_Identity=25.2032520325203, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17559526, Length=535, Percent_Identity=26.1682242990654, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71983001, Length=558, Percent_Identity=27.5985663082437, Blast_Score=154, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71982997, Length=558, Percent_Identity=27.5985663082437, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17558820, Length=521, Percent_Identity=25.7197696737044, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32563687, Length=513, Percent_Identity=25.9259259259259, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17557194, Length=528, Percent_Identity=25.9469696969697, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17538037, Length=538, Percent_Identity=24.907063197026, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI71994703, Length=522, Percent_Identity=25.8620689655172, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71994694, Length=522, Percent_Identity=25.8620689655172, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71994690, Length=522, Percent_Identity=25.8620689655172, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560308, Length=536, Percent_Identity=24.8134328358209, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=493, Percent_Identity=24.1379310344828, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71985884, Length=380, Percent_Identity=26.8421052631579, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=527, Percent_Identity=23.719165085389, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=495, Percent_Identity=23.2323232323232, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115534440, Length=542, Percent_Identity=23.9852398523985, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319264, Length=564, Percent_Identity=27.3049645390071, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6323182, Length=561, Percent_Identity=25.8467023172906, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6319699, Length=348, Percent_Identity=26.1494252873563, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24667955, Length=560, Percent_Identity=28.3928571428571, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI62472339, Length=527, Percent_Identity=28.6527514231499, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24667959, Length=365, Percent_Identity=32.8767123287671, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI21355225, Length=521, Percent_Identity=26.8714011516315, Blast_Score=165, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24581924, Length=534, Percent_Identity=26.9662921348315, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21355181, Length=502, Percent_Identity=26.4940239043825, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI18859661, Length=508, Percent_Identity=26.9685039370079, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19922652, Length=504, Percent_Identity=23.6111111111111, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24656500, Length=505, Percent_Identity=23.3663366336634, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356947, Length=483, Percent_Identity=21.7391304347826, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648260, Length=358, Percent_Identity=26.2569832402235, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24653035, Length=364, Percent_Identity=24.1758241758242, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI20130357, Length=355, Percent_Identity=26.1971830985915, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648257, Length=504, Percent_Identity=23.015873015873, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21356441, Length=374, Percent_Identity=22.4598930481283, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI161076582, Length=368, Percent_Identity=23.3695652173913, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI28573631, Length=543, Percent_Identity=22.0994475138122, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI20130353, Length=543, Percent_Identity=22.0994475138122, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281365686, Length=318, Percent_Identity=21.0691823899371, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648253, Length=376, Percent_Identity=22.6063829787234, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648255, Length=388, Percent_Identity=22.1649484536082, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21358303, Length=322, Percent_Identity=21.1180124223602, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.1

Molecular weight: Translated: 59978; Mature: 59978

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMKREELLAPPSYNLVSEIEKYTGDKGKLALIWQDDKGNRREVTYAELMKGANKIGNAF
CCCCCHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
IKSGLQKGDKLLIMMPRLIEAYMTYIAAIKAGFVVIPSSEMLRKKDIEYRIGHGEVKAIV
HHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCEEEECCHHHHHHCCCCEEECCCCEEEEE
SYEPYIGQFDDIEAMESLQKFVLSEQSVDGWINLKTALETESDMLEMAKTDKEDMVFLSY
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEHHCCHHHHHHHHHCCCCCEEEEEE
TSGTTGNPKGVVHTHAWAYAHLRTSAPNWLGIEENDVVWATASPGWQKWIWSPFLATLGS
CCCCCCCCCCEEEECEEEEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCC
GATGFVYHGKFEPKTYLNLLDDNKVNVLCCTPTEYRLMAKVENLSEYNLEALHSAVSAGE
CCCEEEEECCCCCHHHHEEECCCCEEEEEECCCCHHHHHHHHCCHHCCHHHHHHHHHCCC
PLNREVIETFQRHFHITVRDGYGQTENTLLVGVMKGMDIRPGSMGKPTPGNHVDIVNEEG
CCCHHHHHHHHHCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCC
IPVQVGEVGDIAVHIETPALFKQYYKDDERTAMQFRGDYYITGDKAKKDEDGYFWFEGRG
CEEEECCCCEEEEEEECHHHHHHHHCCCCHHEEEECCCEEEECCCCCCCCCCEEEEECCC
DDIIISSGYTIGPFEVEDALVKHPYVRECAVVASPDEIRGSVVKAFIVLRENIEKNEETL
CEEEEECCCEECCCHHHHHHHCCCCHHHEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHH
IPILQQHVKELTAPYKYPRKIEFVDELPKTISGKIRRIELRKQEMEIPSK
HHHHHHHHHHHHCCCCCCCEEHHHHHCCHHHCCCEEEEEEEHHHCCCCCC
>Mature Secondary Structure
MFMKREELLAPPSYNLVSEIEKYTGDKGKLALIWQDDKGNRREVTYAELMKGANKIGNAF
CCCCCHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
IKSGLQKGDKLLIMMPRLIEAYMTYIAAIKAGFVVIPSSEMLRKKDIEYRIGHGEVKAIV
HHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCEEEECCHHHHHHCCCCEEECCCCEEEEE
SYEPYIGQFDDIEAMESLQKFVLSEQSVDGWINLKTALETESDMLEMAKTDKEDMVFLSY
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEHHCCHHHHHHHHHCCCCCEEEEEE
TSGTTGNPKGVVHTHAWAYAHLRTSAPNWLGIEENDVVWATASPGWQKWIWSPFLATLGS
CCCCCCCCCCEEEECEEEEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCC
GATGFVYHGKFEPKTYLNLLDDNKVNVLCCTPTEYRLMAKVENLSEYNLEALHSAVSAGE
CCCEEEEECCCCCHHHHEEECCCCEEEEEECCCCHHHHHHHHCCHHCCHHHHHHHHHCCC
PLNREVIETFQRHFHITVRDGYGQTENTLLVGVMKGMDIRPGSMGKPTPGNHVDIVNEEG
CCCHHHHHHHHHCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCC
IPVQVGEVGDIAVHIETPALFKQYYKDDERTAMQFRGDYYITGDKAKKDEDGYFWFEGRG
CEEEECCCCEEEEEEECHHHHHHHHCCCCHHEEEECCCEEEECCCCCCCCCCEEEEECCC
DDIIISSGYTIGPFEVEDALVKHPYVRECAVVASPDEIRGSVVKAFIVLRENIEKNEETL
CEEEEECCCEECCCHHHHHHHCCCCHHHEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHH
IPILQQHVKELTAPYKYPRKIEFVDELPKTISGKIRRIELRKQEMEIPSK
HHHHHHHHHHHHCCCCCCCEEHHHHHCCHHHCCCEEEEEEEHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; acetate; CoA

Specific reaction: ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA

General reaction: Acid-thiol ligation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]