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The map label for this gene is pykA [H]

Identifier: 118479744

GI number: 118479744

Start: 4403774

End: 4405576

Strand: Reverse

Name: pykA [H]

Synonym: BALH_4181

Alternate gene names: 118479744

Gene position: 4405576-4403774 (Counterclockwise)

Preceding gene: 118479745

Following gene: 118479742

Centisome position: 83.8

GC content: 40.77

Gene sequence:

>1803_bases
ATGTCGGATATTTGGGAACGGTTTCATAATTTTCGGAGGTGCAATATGCGTAAAACTAAAATTGTATGTACTATAGGTCC
TGCTAGTGAAAGTATTGAAAAATTAGAGCAATTAATCGAAGCGGGTATGAACGTTGCTCGTTTAAACTTCTCTCATGGTA
GCCATGAAGAGCACGGCGCTCGTATTAAAAACATTCGTGAAGCTTCAAAGAAAACTGGTAAAACAGTTGGTATCTTACTT
GATACAAAAGGTCCAGAAATCCGTACTCACGACTTCGTAGACGGACAAGCTGAGCTTGTAACAGGTGCAGAAGTAGTTCT
TTCTACTGAGCAAGTATTAGGTACTGCAGAGAAGTTCTCTGTATCTTATGCTGGTCTTTATGACGATGTAGACCCAGGTT
CTCGCATTCTAATCGATGACGGTCTTATCGAGCTAGAAGTAATCGAAAAAGCTGACGGAAACATCCGTACAAAAGTTCTT
AACAGCGGAACTGTAAAAAATAAAAAAGGTGTTAACGTACCAAACGTAAGCATTAAGCTTCCTGGTATCACTGAAAAAGA
TGTAAAAGATATCATCTTCGGTATCGAGCAAAAAGTTGATTTCATCGCAGCTTCATTCGTACGTAAAGCAGCTGACGTAT
TAGAAATCCGTGAATTATTAGAAGAGCATAACGCTCAATACATCCAAATCGTACCAAAAATTGAAAACCAAGAGGGTATC
GACAACATCGATTCAATCTTAGAAGTTTCTGACGGTTTAATGGTAGCTCGTGGTGACATGGGTGTAGAAATTCCGCCAGA
AGAAGTACCATTAGTACAAAAACGTCTAATCAAAAAATGTAACGTGTTAGGTAAACCAGTTATTACTGCAACACAAATGT
TAGATTCTATGCAACGTAACCCACGTCCAACTCGTGCGGAAGCAAGTGACGTAGCTAACGCAATCTTCGATGGTACAGAT
GCAATCATGCTTTCAGGTGAAACGGCTGCTGGACAATACCCAGTAGAAGCAGTAACAATGATGGCTAATATTGCAGTACG
TGTTGAAAAATCATTACAATATGAAGATATGTTCAAAAAACGTATTAAAGAGTTCACTCCAACAATTACAGATGCAATTA
GCCAATCTGTTGCGCATACAGCACTTGCTCTTGATGTAGCTGCAATCGTAGCTCCAACAGAAAGTGGATATACTGCGAAA
ATGATCTCTAAATACCGTCCAAAATCTCCAATCGTAGCTGTAACATCTGACGAGCAAGTAGGACGTCGTCTTGCGCTTGT
TTGGGGTGTACAAGCATTTATGGCTGAGAAGCGTGCGGCTTCTACTGACGAAATGTTAGATACAGCGATTCAAACAGGTA
TGGATGCAGGTCTAATCGGACTTGGAGACACTGTAGTAATCACTGCTGGTGTTCCAGTTGCTGAAACTGGTACAACAAAC
TTAATGAAAATCCACGTTGTTGGTGAAGAAGTTGCTAAAGGACAAGGAATCGGTCGTAAAGCTGCAAAAGGTAAAGTAGT
TGTAGCGAAAACAGCTGCTGAAGCTGTAGCGAACGTAAACGAAGGTGATATCCTTGTTACAACAAGCACTGATAAAGATA
TGATTCCTGCAATCGAAAAAGCTGCTGCTCTAGTTGTAGAAGAAGGCGGCCTAACAAGCCATGCAGCTGTTGTAGGCGTA
TCAATCGGCATTCCTGTTATCGTTGGTGTAAACGGCGTAACAGCAACTTTAAAAAATGGCCAAGAAGTAACAGTTGATGC
AGCACGCGGAATTGTTTATAATGGACATGCGGAAGTGCTATAA

Upstream 100 bases:

>100_bases
AACTTGTTGATCATGACATTATCGAAGCGTTAGCTCAAAAGCATACAATCGATAAAGATATGTATCAATTATCTAAAGAA
TTATCCATCTAATCGGCGTA

Downstream 100 bases:

>100_bases
GTAATACGGAGAGAAGGATCGGAGTCCTTCTCTTTTTTTTACACAAAAATGAGGTAAAACTAGCTTAAGACGTCTGAAAT
TGCTTTTAAAAGCTGTTTAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK; Vegetative protein 17; VEG17 [H]

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MSDIWERFHNFRRCNMRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGARIKNIREASKKTGKTVGILL
DTKGPEIRTHDFVDGQAELVTGAEVVLSTEQVLGTAEKFSVSYAGLYDDVDPGSRILIDDGLIELEVIEKADGNIRTKVL
NSGTVKNKKGVNVPNVSIKLPGITEKDVKDIIFGIEQKVDFIAASFVRKAADVLEIRELLEEHNAQYIQIVPKIENQEGI
DNIDSILEVSDGLMVARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTD
AIMLSGETAAGQYPVEAVTMMANIAVRVEKSLQYEDMFKKRIKEFTPTITDAISQSVAHTALALDVAAIVAPTESGYTAK
MISKYRPKSPIVAVTSDEQVGRRLALVWGVQAFMAEKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTN
LMKIHVVGEEVAKGQGIGRKAAKGKVVVAKTAAEAVANVNEGDILVTTSTDKDMIPAIEKAAALVVEEGGLTSHAAVVGV
SIGIPVIVGVNGVTATLKNGQEVTVDAARGIVYNGHAEVL

Sequences:

>Translated_600_residues
MSDIWERFHNFRRCNMRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGARIKNIREASKKTGKTVGILL
DTKGPEIRTHDFVDGQAELVTGAEVVLSTEQVLGTAEKFSVSYAGLYDDVDPGSRILIDDGLIELEVIEKADGNIRTKVL
NSGTVKNKKGVNVPNVSIKLPGITEKDVKDIIFGIEQKVDFIAASFVRKAADVLEIRELLEEHNAQYIQIVPKIENQEGI
DNIDSILEVSDGLMVARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTD
AIMLSGETAAGQYPVEAVTMMANIAVRVEKSLQYEDMFKKRIKEFTPTITDAISQSVAHTALALDVAAIVAPTESGYTAK
MISKYRPKSPIVAVTSDEQVGRRLALVWGVQAFMAEKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTN
LMKIHVVGEEVAKGQGIGRKAAKGKVVVAKTAAEAVANVNEGDILVTTSTDKDMIPAIEKAAALVVEEGGLTSHAAVVGV
SIGIPVIVGVNGVTATLKNGQEVTVDAARGIVYNGHAEVL
>Mature_599_residues
SDIWERFHNFRRCNMRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGARIKNIREASKKTGKTVGILLD
TKGPEIRTHDFVDGQAELVTGAEVVLSTEQVLGTAEKFSVSYAGLYDDVDPGSRILIDDGLIELEVIEKADGNIRTKVLN
SGTVKNKKGVNVPNVSIKLPGITEKDVKDIIFGIEQKVDFIAASFVRKAADVLEIRELLEEHNAQYIQIVPKIENQEGID
NIDSILEVSDGLMVARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDA
IMLSGETAAGQYPVEAVTMMANIAVRVEKSLQYEDMFKKRIKEFTPTITDAISQSVAHTALALDVAAIVAPTESGYTAKM
ISKYRPKSPIVAVTSDEQVGRRLALVWGVQAFMAEKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTNL
MKIHVVGEEVAKGQGIGRKAAKGKVVVAKTAAEAVANVNEGDILVTTSTDKDMIPAIEKAAALVVEEGGLTSHAAVVGVS
IGIPVIVGVNGVTATLKNGQEVTVDAARGIVYNGHAEVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=489, Percent_Identity=44.3762781186094, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=43.6734693877551, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=43.6734693877551, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI32967597, Length=489, Percent_Identity=44.3762781186094, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI33286418, Length=490, Percent_Identity=43.8775510204082, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=47.766323024055, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=47.766323024055, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=44.7698744769874, Blast_Score=196, Evalue=6e-50,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=44.7698744769874, Blast_Score=196, Evalue=6e-50,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=43.1192660550459, Blast_Score=166, Evalue=5e-41,
Organism=Escherichia coli, GI1787965, Length=474, Percent_Identity=50.210970464135, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=40.1247401247401, Blast_Score=296, Evalue=4e-81,
Organism=Escherichia coli, GI1787994, Length=117, Percent_Identity=38.4615384615385, Blast_Score=83, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=44.7916666666667, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=45.6611570247934, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=45.6611570247934, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=45.6611570247934, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=45.4545454545455, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324923, Length=489, Percent_Identity=41.5132924335378, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=41.4784394250513, Blast_Score=350, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=43.9672801635992, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI28571814, Length=489, Percent_Identity=43.9672801635992, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=47.0731707317073, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24581235, Length=491, Percent_Identity=35.234215885947, Blast_Score=289, Evalue=5e-78,
Organism=Drosophila melanogaster, GI24646914, Length=306, Percent_Identity=41.5032679738562, Blast_Score=214, Evalue=1e-55,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 64232; Mature: 64100

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIWERFHNFRRCNMRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGA
CCHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RIKNIREASKKTGKTVGILLDTKGPEIRTHDFVDGQAELVTGAEVVLSTEQVLGTAEKFS
HHHHHHHHHHHCCCEEEEEEECCCCCCEECCCCCCCCEEEECHHHEEEHHHHHCCHHHHE
VSYAGLYDDVDPGSRILIDDGLIELEVIEKADGNIRTKVLNSGTVKNKKGVNVPNVSIKL
EEEECCCCCCCCCCEEEEECCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEC
PGITEKDVKDIIFGIEQKVDFIAASFVRKAADVLEIRELLEEHNAQYIQIVPKIENQEGI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCC
DNIDSILEVSDGLMVARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRN
HHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
PRPTRAEASDVANAIFDGTDAIMLSGETAAGQYPVEAVTMMANIAVRVEKSLQYEDMFKK
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
RIKEFTPTITDAISQSVAHTALALDVAAIVAPTESGYTAKMISKYRPKSPIVAVTSDEQV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCCCCEEEEECCHHH
GRRLALVWGVQAFMAEKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTN
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCEEEEEECCCEECCCCCC
LMKIHVVGEEVAKGQGIGRKAAKGKVVVAKTAAEAVANVNEGDILVTTSTDKDMIPAIEK
EEEEEEECHHHHCCCCCCCCCCCCCEEEEECHHHHHHCCCCCCEEEEECCCCCHHHHHHH
AAALVVEEGGLTSHAAVVGVSIGIPVIVGVNGVTATLKNGQEVTVDAARGIVYNGHAEVL
HHEEEEECCCCCCCCEEEEEEECCEEEEECCCEEEEECCCCEEEEEECCCEEECCCCCCC
>Mature Secondary Structure 
SDIWERFHNFRRCNMRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGA
CHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RIKNIREASKKTGKTVGILLDTKGPEIRTHDFVDGQAELVTGAEVVLSTEQVLGTAEKFS
HHHHHHHHHHHCCCEEEEEEECCCCCCEECCCCCCCCEEEECHHHEEEHHHHHCCHHHHE
VSYAGLYDDVDPGSRILIDDGLIELEVIEKADGNIRTKVLNSGTVKNKKGVNVPNVSIKL
EEEECCCCCCCCCCEEEEECCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEC
PGITEKDVKDIIFGIEQKVDFIAASFVRKAADVLEIRELLEEHNAQYIQIVPKIENQEGI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCC
DNIDSILEVSDGLMVARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRN
HHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
PRPTRAEASDVANAIFDGTDAIMLSGETAAGQYPVEAVTMMANIAVRVEKSLQYEDMFKK
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
RIKEFTPTITDAISQSVAHTALALDVAAIVAPTESGYTAKMISKYRPKSPIVAVTSDEQV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCCCCEEEEECCHHH
GRRLALVWGVQAFMAEKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTN
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCEEEEEECCCEECCCCCC
LMKIHVVGEEVAKGQGIGRKAAKGKVVVAKTAAEAVANVNEGDILVTTSTDKDMIPAIEK
EEEEEEECHHHHCCCCCCCCCCCCCEEEEECHHHHHHCCCCCCEEEEECCCCCHHHHHHH
AAALVVEEGGLTSHAAVVGVSIGIPVIVGVNGVTATLKNGQEVTVDAARGIVYNGHAEVL
HHEEEEECCCCCCCCEEEEEEECCEEEEECCCEEEEECCCCEEEEEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 9298659 [H]