BacMap

Definition	Bacillus thuringiensis str. Al Hakam chromosome, complete genome.
Accession	NC_008600
Length	5,257,091

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The map label for this gene is phaB [H]

Identifier: 118479676

GI number: 118479676

Start: 4324935

End: 4325741

Strand: Reverse

Name: phaB [H]

Synonym: BALH_4108

Alternate gene names: 118479676

Gene position: 4325741-4324935 (Counterclockwise)

Preceding gene: 118479677

Following gene: 118479675

Centisome position: 82.28

GC content: 42.13

Gene sequence:

>807_bases
TTGTGGTTATCGTCAATAATGGGAGGGATCATGTTGAAATTCCTATCTGTAAGAGTTGAAGATCATATCGCGGTGGCTAC
GTTAAATCATGCGCCAGCTAACGCGATGTCTTCACAAGTTATGCATGACGTTACTGAATTAATTGATCAAGTGGAGAAGG
ATGATAATATTCGTGTTGTTGTTATTCACGGTGAAGGGCGCTTCTTCTCGGCGGGAGCAGATATTAAAGAATTTACATCT
GTTACTGAAGCGAAGCAAGCGACTGAATTAGCGCAGCTTGGACAAGTTACGTTTGAGCGCGTGGAAAAATGTTCAAAACC
AGTTATTGCGGCAATTCATGGAGCGGCACTTGGCGGCGGCCTTGAGTTTGCTATGTCTTGCCACATGCGCTTTGCAACTG
AAAGTGCAAAACTTGGTTTACCTGAATTAACACTTGGATTAATTCCTGGATTTGCAGGTACACAGCGATTACCACGTTAT
GTTGGGAAAGCGAAAGCTTGTGAAATGATGTTAACAAGTACACCGATTACTGGTGCAGAAGCATTAAAATGGGGACTAGT
TAACGGAGTTTTTGCTGAAGAAACATTTTTAGATGATACGCTTAAAGTTGCAAAACAGATTGCTGGAAAAAGCCCAGCGA
CGACTCGTGCTGTACTAGAGTTATTGCAAACGACGAAATCGTCTCATTATTATGAGGGTGTACAACGCGAATCTCAAATC
TTTGGTGAAGTATTTACGAGTGAAGATGGAAGAGAAGGTGTAGCAGCGTTTTTAGAAAAACGTAAGCCTTCATTTAGTGG
CAGGTAG

Upstream 100 bases:

>100_bases
TTGGAACAGTAGATGAAGTTGTGACCAATTGGGTAATGAGCGATCATAAGTATGATCTGGTCGCACTTTCAAAAACGGTA
CATGGGCTACTTATTGCAGC

Downstream 100 bases:

>100_bases
TTCAATTATTTTTTTAATATAAATTAACAGAAAATTAAAATTGATAGAATCTTTCTGAAAAACAAGGGGGTAAATGGAAT
GAACATCTACGTACTCATGA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MWLSSIMGGIMLKFLSVRVEDHIAVATLNHAPANAMSSQVMHDVTELIDQVEKDDNIRVVVIHGEGRFFSAGADIKEFTS
VTEAKQATELAQLGQVTFERVEKCSKPVIAAIHGAALGGGLEFAMSCHMRFATESAKLGLPELTLGLIPGFAGTQRLPRY
VGKAKACEMMLTSTPITGAEALKWGLVNGVFAEETFLDDTLKVAKQIAGKSPATTRAVLELLQTTKSSHYYEGVQRESQI
FGEVFTSEDGREGVAAFLEKRKPSFSGR

Sequences:

>Translated_268_residues
MWLSSIMGGIMLKFLSVRVEDHIAVATLNHAPANAMSSQVMHDVTELIDQVEKDDNIRVVVIHGEGRFFSAGADIKEFTS
VTEAKQATELAQLGQVTFERVEKCSKPVIAAIHGAALGGGLEFAMSCHMRFATESAKLGLPELTLGLIPGFAGTQRLPRY
VGKAKACEMMLTSTPITGAEALKWGLVNGVFAEETFLDDTLKVAKQIAGKSPATTRAVLELLQTTKSSHYYEGVQRESQI
FGEVFTSEDGREGVAAFLEKRKPSFSGR
>Mature_268_residues
MWLSSIMGGIMLKFLSVRVEDHIAVATLNHAPANAMSSQVMHDVTELIDQVEKDDNIRVVVIHGEGRFFSAGADIKEFTS
VTEAKQATELAQLGQVTFERVEKCSKPVIAAIHGAALGGGLEFAMSCHMRFATESAKLGLPELTLGLIPGFAGTQRLPRY
VGKAKACEMMLTSTPITGAEALKWGLVNGVFAEETFLDDTLKVAKQIAGKSPATTRAVLELLQTTKSSHYYEGVQRESQI
FGEVFTSEDGREGVAAFLEKRKPSFSGR

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=34.5238095238095, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI68989263, Length=207, Percent_Identity=35.7487922705314, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI20127408, Length=175, Percent_Identity=40.5714285714286, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI70995211, Length=234, Percent_Identity=31.1965811965812, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=29.8804780876494, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI4502327, Length=251, Percent_Identity=31.0756972111554, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI221136756, Length=256, Percent_Identity=25.390625, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI221136753, Length=256, Percent_Identity=25.390625, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI221307494, Length=256, Percent_Identity=25.390625, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI213417737, Length=246, Percent_Identity=25.2032520325203, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI157694516, Length=246, Percent_Identity=25.2032520325203, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI150378541, Length=240, Percent_Identity=29.5833333333333, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI25453481, Length=235, Percent_Identity=23.4042553191489, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI51538573, Length=235, Percent_Identity=23.4042553191489, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI45643119, Length=252, Percent_Identity=25.3968253968254, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI260275230, Length=252, Percent_Identity=25.3968253968254, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI260274832, Length=252, Percent_Identity=25.3968253968254, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI157694520, Length=223, Percent_Identity=25.1121076233184, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4757966, Length=224, Percent_Identity=23.6607142857143, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI51538581, Length=224, Percent_Identity=23.6607142857143, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4757968, Length=268, Percent_Identity=23.5074626865672, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI49355787, Length=268, Percent_Identity=23.5074626865672, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI261878539, Length=109, Percent_Identity=36.697247706422, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI116235487, Length=251, Percent_Identity=24.3027888446215, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI37594469, Length=148, Percent_Identity=31.7567567567568, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI37594471, Length=213, Percent_Identity=29.1079812206573, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=37.6470588235294, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1788682, Length=271, Percent_Identity=34.6863468634686, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI221142681, Length=247, Percent_Identity=33.6032388663968, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=29.2307692307692, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=30.4182509505703, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1790281, Length=192, Percent_Identity=32.8125, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788597, Length=263, Percent_Identity=28.1368821292776, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=38.4920634920635, Blast_Score=171, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17554946, Length=259, Percent_Identity=35.5212355212355, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17540714, Length=227, Percent_Identity=33.4801762114537, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17558304, Length=181, Percent_Identity=36.4640883977901, Blast_Score=117, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17534483, Length=274, Percent_Identity=29.5620437956204, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17508951, Length=181, Percent_Identity=37.5690607734807, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=181, Percent_Identity=37.5690607734807, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17508953, Length=181, Percent_Identity=37.5690607734807, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=258, Percent_Identity=30.2325581395349, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17540306, Length=241, Percent_Identity=28.6307053941909, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=255, Percent_Identity=25.0980392156863, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=234, Percent_Identity=27.7777777777778, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=24.5059288537549, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=226, Percent_Identity=24.3362831858407, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=237, Percent_Identity=26.5822784810127, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=38.6178861788618, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=38.6178861788618, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=37.0517928286853, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI19920382, Length=253, Percent_Identity=30.4347826086957, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI19921000, Length=181, Percent_Identity=37.5690607734807, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24583077, Length=181, Percent_Identity=37.5690607734807, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24583079, Length=181, Percent_Identity=37.5690607734807, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922422, Length=263, Percent_Identity=29.277566539924, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357171, Length=266, Percent_Identity=30.8270676691729, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24650670, Length=196, Percent_Identity=34.1836734693878, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24654903, Length=184, Percent_Identity=26.0869565217391, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24583165, Length=147, Percent_Identity=29.9319727891156, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 29024; Mature: 29024

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWLSSIMGGIMLKFLSVRVEDHIAVATLNHAPANAMSSQVMHDVTELIDQVEKDDNIRVV
CCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VIHGEGRFFSAGADIKEFTSVTEAKQATELAQLGQVTFERVEKCSKPVIAAIHGAALGGG
EEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCH
LEFAMSCHMRFATESAKLGLPELTLGLIPGFAGTQRLPRYVGKAKACEMMLTSTPITGAE
HHHHHHHHHHHHCCHHCCCCCHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCHHH
ALKWGLVNGVFAEETFLDDTLKVAKQIAGKSPATTRAVLELLQTTKSSHYYEGVQRESQI
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGEVFTSEDGREGVAAFLEKRKPSFSGR
HHHHHCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MWLSSIMGGIMLKFLSVRVEDHIAVATLNHAPANAMSSQVMHDVTELIDQVEKDDNIRVV
CCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VIHGEGRFFSAGADIKEFTSVTEAKQATELAQLGQVTFERVEKCSKPVIAAIHGAALGGG
EEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCH
LEFAMSCHMRFATESAKLGLPELTLGLIPGFAGTQRLPRYVGKAKACEMMLTSTPITGAE
HHHHHHHHHHHHCCHHCCCCCHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCHHH
ALKWGLVNGVFAEETFLDDTLKVAKQIAGKSPATTRAVLELLQTTKSSHYYEGVQRESQI
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGEVFTSEDGREGVAAFLEKRKPSFSGR
HHHHHCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]