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The map label for this gene is bct [H]

Identifier: 118479649

GI number: 118479649

Start: 4299052

End: 4300506

Strand: Reverse

Name: bct [H]

Synonym: BALH_4076

Alternate gene names: 118479649

Gene position: 4300506-4299052 (Counterclockwise)

Preceding gene: 118479651

Following gene: 118479648

Centisome position: 81.8

GC content: 34.02

Gene sequence:

>1455_bases
ATGTTTCGTTTCGAATTCAATTTGTCGCGCGATAAATGTATACATTTGATGAAGTTGTTTAGCAGCGATAGAAGCGCTAA
ACAACTTTATTTTCATTTACAGTTACTACAATCAGGAGAGGTGTTACACATGGGGAAAGATATAAGTAACCATTCAAAAT
GGTTTGTTTTCACATTATGTTTTATCGTTTTATTAGGACCGGTGAATGCGGTTTTATTTAATGTTGCGTTAGAGGATATG
GCTCATGATTTATCCATTAGTCAATCGAAAGTAAGTTGGGTTGTAGTAGGTTACTCCTTAGTTGTCGGTATTGGTTCGAT
GATATATGGGAAACTGGCTGATCGTTATAGTGTGAAAAAACTATTAATTATTTCAATCATCATATTTGTAGCGGGTTCTA
TTATTGGATTTGTAAATCAATCATATGCGATTGTCATTTTTGCAAGATTAGTGCAGGCGAGTGGGGGCGCGGCGTTTATT
GCGCTTAGTATGATTGCGGTAGCAAAATTAGTTGCTCCTGCTAAGAAGCCTGGTGCTTTAGCGATGATTAGTTCTTCTAT
TGCGTTAGCGGTTGGTATTGGTCCTTTAGTTGGTGGGGCTATTACAAATACACTAGGGTGGCCATATTTATTTTTATTTA
TGATTATCTCAGTATTGGGGATTTTCTTGCTTATAAAATTTATGCCAGGAGAAGCGCATCATACGGATGAAGTGTTTTAT
TTTGATTACATTGGAGCGGCGTTACTATTTGTATTTATTACGACTGTTTTGGTAGGTGTTAATATGAATAGTTGGCTATT
TGTGTTATCGATAATTTCCTTATTTTTATTCACGGTTCGTATGAAGAAAGCGGAGCACCCATTTATCGATATTGAGTTAT
TTTCGAACAAAGCATTTCTTCGTTTAATAACAGTCGGATTTATAATTAATGTGGCGTTATGTGCTAATTTATTATTATTG
CCATTACTGTTAGGAAGAGTACACGGATTGTCACCGTTTATTATCGGAATTGTATTATTTGTTGCATCACTTTTCGGTAT
TGTGTCTAGTTTTATTACTGGAAAGATTATCCCTTCGTTTGGAAATGTGAATATGATTTATGTAGCGTCTGTCATTATGA
TTGTTGGCTTTTTAATTTTAGGGTTTATTCCGAATGGAAGTATAGTCGTTATTGTATTGGCGATTATTTTAACGTTTATG
AGTTATTCTGCCATTCAAGTATCATTGAACACATTTATACCGAAAACATTACATGTAGCTAAAGTTGGAGTCGGTCTTGG
TTTATATAATTTAATTAACTTTTTCGGTATGGCATTTGGACCAGCTGTAGCGAGCAAAATTATGGAATCCACAAATAGTT
ATCGTTTTAATTTTATTTTAATCGTCATGTTAATTTCTGCTCATTTCTTCTTATTAATAGGAATGTCTTCTTTCCAAAAA
AAGATGGAGCATTAA

Upstream 100 bases:

>100_bases
AAGTAAACTAAAAACACTAAATTAGTTCACTTTGTATATTTTTCGTTGACAAACTGGAATGCTGAAACTATGATAGTTCC
ATGAATATCGAACAACGTAC

Downstream 100 bases:

>100_bases
ATATGTACTTTATATAGAAGAAACCGACTTTCCTATAGAAGGTCGGTTATTTTTCTGTCTTGCAAATTATGAAAAAAAGG
AGCAAAATAAAAAAGGTCCT

Product: tetracycline resistence protein, metal-tetracycline/H+ antiporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MFRFEFNLSRDKCIHLMKLFSSDRSAKQLYFHLQLLQSGEVLHMGKDISNHSKWFVFTLCFIVLLGPVNAVLFNVALEDM
AHDLSISQSKVSWVVVGYSLVVGIGSMIYGKLADRYSVKKLLIISIIIFVAGSIIGFVNQSYAIVIFARLVQASGGAAFI
ALSMIAVAKLVAPAKKPGALAMISSSIALAVGIGPLVGGAITNTLGWPYLFLFMIISVLGIFLLIKFMPGEAHHTDEVFY
FDYIGAALLFVFITTVLVGVNMNSWLFVLSIISLFLFTVRMKKAEHPFIDIELFSNKAFLRLITVGFIINVALCANLLLL
PLLLGRVHGLSPFIIGIVLFVASLFGIVSSFITGKIIPSFGNVNMIYVASVIMIVGFLILGFIPNGSIVVIVLAIILTFM
SYSAIQVSLNTFIPKTLHVAKVGVGLGLYNLINFFGMAFGPAVASKIMESTNSYRFNFILIVMLISAHFFLLIGMSSFQK
KMEH

Sequences:

>Translated_484_residues
MFRFEFNLSRDKCIHLMKLFSSDRSAKQLYFHLQLLQSGEVLHMGKDISNHSKWFVFTLCFIVLLGPVNAVLFNVALEDM
AHDLSISQSKVSWVVVGYSLVVGIGSMIYGKLADRYSVKKLLIISIIIFVAGSIIGFVNQSYAIVIFARLVQASGGAAFI
ALSMIAVAKLVAPAKKPGALAMISSSIALAVGIGPLVGGAITNTLGWPYLFLFMIISVLGIFLLIKFMPGEAHHTDEVFY
FDYIGAALLFVFITTVLVGVNMNSWLFVLSIISLFLFTVRMKKAEHPFIDIELFSNKAFLRLITVGFIINVALCANLLLL
PLLLGRVHGLSPFIIGIVLFVASLFGIVSSFITGKIIPSFGNVNMIYVASVIMIVGFLILGFIPNGSIVVIVLAIILTFM
SYSAIQVSLNTFIPKTLHVAKVGVGLGLYNLINFFGMAFGPAVASKIMESTNSYRFNFILIVMLISAHFFLLIGMSSFQK
KMEH
>Mature_484_residues
MFRFEFNLSRDKCIHLMKLFSSDRSAKQLYFHLQLLQSGEVLHMGKDISNHSKWFVFTLCFIVLLGPVNAVLFNVALEDM
AHDLSISQSKVSWVVVGYSLVVGIGSMIYGKLADRYSVKKLLIISIIIFVAGSIIGFVNQSYAIVIFARLVQASGGAAFI
ALSMIAVAKLVAPAKKPGALAMISSSIALAVGIGPLVGGAITNTLGWPYLFLFMIISVLGIFLLIKFMPGEAHHTDEVFY
FDYIGAALLFVFITTVLVGVNMNSWLFVLSIISLFLFTVRMKKAEHPFIDIELFSNKAFLRLITVGFIINVALCANLLLL
PLLLGRVHGLSPFIIGIVLFVASLFGIVSSFITGKIIPSFGNVNMIYVASVIMIVGFLILGFIPNGSIVVIVLAIILTFM
SYSAIQVSLNTFIPKTLHVAKVGVGLGLYNLINFFGMAFGPAVASKIMESTNSYRFNFILIVMLISAHFFLLIGMSSFQK
KMEH

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=436, Percent_Identity=22.9357798165138, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788710, Length=280, Percent_Identity=25, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789042, Length=308, Percent_Identity=25, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87081983, Length=305, Percent_Identity=24.9180327868852, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788392, Length=207, Percent_Identity=25.1207729468599, Blast_Score=62, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=123, Percent_Identity=34.9593495934959, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=171, Percent_Identity=26.9005847953216, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=135, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53035; Mature: 53035

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRFEFNLSRDKCIHLMKLFSSDRSAKQLYFHLQLLQSGEVLHMGKDISNHSKWFVFTLC
CEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
FIVLLGPVNAVLFNVALEDMAHDLSISQSKVSWVVVGYSLVVGIGSMIYGKLADRYSVKK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHH
LLIISIIIFVAGSIIGFVNQSYAIVIFARLVQASGGAAFIALSMIAVAKLVAPAKKPGAL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCH
AMISSSIALAVGIGPLVGGAITNTLGWPYLFLFMIISVLGIFLLIKFMPGEAHHTDEVFY
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEH
FDYIGAALLFVFITTVLVGVNMNSWLFVLSIISLFLFTVRMKKAEHPFIDIELFSNKAFL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHH
RLITVGFIINVALCANLLLLPLLLGRVHGLSPFIIGIVLFVASLFGIVSSFITGKIIPSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GNVNMIYVASVIMIVGFLILGFIPNGSIVVIVLAIILTFMSYSAIQVSLNTFIPKTLHVA
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEHHHHCCHHHHHH
KVGVGLGLYNLINFFGMAFGPAVASKIMESTNSYRFNFILIVMLISAHFFLLIGMSSFQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KMEH
HHCC
>Mature Secondary Structure
MFRFEFNLSRDKCIHLMKLFSSDRSAKQLYFHLQLLQSGEVLHMGKDISNHSKWFVFTLC
CEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
FIVLLGPVNAVLFNVALEDMAHDLSISQSKVSWVVVGYSLVVGIGSMIYGKLADRYSVKK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHH
LLIISIIIFVAGSIIGFVNQSYAIVIFARLVQASGGAAFIALSMIAVAKLVAPAKKPGAL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCH
AMISSSIALAVGIGPLVGGAITNTLGWPYLFLFMIISVLGIFLLIKFMPGEAHHTDEVFY
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEH
FDYIGAALLFVFITTVLVGVNMNSWLFVLSIISLFLFTVRMKKAEHPFIDIELFSNKAFL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHH
RLITVGFIINVALCANLLLLPLLLGRVHGLSPFIIGIVLFVASLFGIVSSFITGKIIPSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GNVNMIYVASVIMIVGFLILGFIPNGSIVVIVLAIILTFMSYSAIQVSLNTFIPKTLHVA
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEHHHHCCHHHHHH
KVGVGLGLYNLINFFGMAFGPAVASKIMESTNSYRFNFILIVMLISAHFFLLIGMSSFQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KMEH
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2844262; 7584024; 9384377 [H]