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The map label for this gene is queA [H]

Identifier: 118479574

GI number: 118479574

Start: 4213978

End: 4215042

Strand: Reverse

Name: queA [H]

Synonym: BALH_3998

Alternate gene names: 118479574

Gene position: 4215042-4213978 (Counterclockwise)

Preceding gene: 118479575

Following gene: 118479573

Centisome position: 80.18

GC content: 38.4

Gene sequence:

>1065_bases
GTGGATATAGTTATGGATATTAATCTGTTTGATTTTCATTTACCAGAAGAACTTATTGCACAAGTCCCATTAGAAGAGCG
TGAAACATCAAGATTAATGGTATTAGACCGCGAAACAGGGGATATTGAGCATAAACATTTTACGGACATTCTTTCTTACT
TACATGAGGGGGATTGCTTAGTTTTAAATGAAACGAAAGTAATGCCAGCTCGTTTGCACGGTGTGAAAGAAGATACAGGC
GCACACATTGAAGTACTTCTTTTAAAACAAGAGGAAGGCGATAAGTGGGAGACGCTTGTTAAGCCAGCGAAACGTGTAAA
AGAAGGAACTGTTATCTCTTTTGGCGAAGGGAAATTAAAAGCAACTTGCACAGGAACTGCAGATCAAGGTGGACGTCAGC
TTGAATTTTCATATGACGGCATCTTCTATGAAATTTTAGATGAACTTGGAGAAATGCCACTTCCTCCATATATTAAAGAG
ACGCTAGAAGATCGCGATCGCTATCAAACAGTATATGCGAAAGAAATCGGTTCAGCAGCAGCGCCAACTGCAGGACTTCA
CTTTACAGAAGAGTTACTGGAGAAGTTAAAACAAAAAGGCGTTGAGTTAGCATTTATTACACTTCACGTAGGGCTTGGAA
CATTTAGACCAGTTTCTGCTGATACGATTGAAGAGCACCATATGCATGCAGAATATTACCATATGTCTGAAGAGACAGCG
GCATTATTAAACCGTGTAAAAGAGAATGGTGGACGTATTATTACGGTAGGTACGACTTCAACTCGTACGTTAGAAACAAT
TGCGACAGATCATAATGGCAAGCTTTGTGCTGCTTCTGGCTGGACAGACATTTTTATGTACCCAGGTTATGAATTTAAAG
CAATTGATGGTTTAATTACAAACTTCCATTTACCGAAATCAACATTAATTATGCTTGTAAGTGCATTTGCAAACAGAGAT
AATGTACTTCATGCTTATAATGAAGCAGTAAAAGAAAAATATCGTTTCTTTAGCTTCGGTGATGCAATGTTCGTTGCATC
TCACGCTAAAATGGGAAACAAATAA

Upstream 100 bases:

>100_bases
GGAAACGTTACCTTTTATTTTCCTATCATTACATGTATTGTGTTAAGTATTGTATTATCTTTTATTATGTATATAATAAA
TCGATTCAAATAAGAAATAG

Downstream 100 bases:

>100_bases
AAGGAGTAAATTATGACAGCAATTCGTTATGAATTTATAAAAACTTGTAAACAAACAGGTGCTCGTTTAGGACGTGTACA
TACGCCGCACGGTTCATTTG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MDIVMDINLFDFHLPEELIAQVPLEERETSRLMVLDRETGDIEHKHFTDILSYLHEGDCLVLNETKVMPARLHGVKEDTG
AHIEVLLLKQEEGDKWETLVKPAKRVKEGTVISFGEGKLKATCTGTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKE
TLEDRDRYQTVYAKEIGSAAAPTAGLHFTEELLEKLKQKGVELAFITLHVGLGTFRPVSADTIEEHHMHAEYYHMSEETA
ALLNRVKENGGRIITVGTTSTRTLETIATDHNGKLCAASGWTDIFMYPGYEFKAIDGLITNFHLPKSTLIMLVSAFANRD
NVLHAYNEAVKEKYRFFSFGDAMFVASHAKMGNK

Sequences:

>Translated_354_residues
MDIVMDINLFDFHLPEELIAQVPLEERETSRLMVLDRETGDIEHKHFTDILSYLHEGDCLVLNETKVMPARLHGVKEDTG
AHIEVLLLKQEEGDKWETLVKPAKRVKEGTVISFGEGKLKATCTGTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKE
TLEDRDRYQTVYAKEIGSAAAPTAGLHFTEELLEKLKQKGVELAFITLHVGLGTFRPVSADTIEEHHMHAEYYHMSEETA
ALLNRVKENGGRIITVGTTSTRTLETIATDHNGKLCAASGWTDIFMYPGYEFKAIDGLITNFHLPKSTLIMLVSAFANRD
NVLHAYNEAVKEKYRFFSFGDAMFVASHAKMGNK
>Mature_354_residues
MDIVMDINLFDFHLPEELIAQVPLEERETSRLMVLDRETGDIEHKHFTDILSYLHEGDCLVLNETKVMPARLHGVKEDTG
AHIEVLLLKQEEGDKWETLVKPAKRVKEGTVISFGEGKLKATCTGTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKE
TLEDRDRYQTVYAKEIGSAAAPTAGLHFTEELLEKLKQKGVELAFITLHVGLGTFRPVSADTIEEHHMHAEYYHMSEETA
ALLNRVKENGGRIITVGTTSTRTLETIATDHNGKLCAASGWTDIFMYPGYEFKAIDGLITNFHLPKSTLIMLVSAFANRD
NVLHAYNEAVKEKYRFFSFGDAMFVASHAKMGNK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=354, Percent_Identity=47.7401129943503, Blast_Score=318, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39845; Mature: 39845

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIVMDINLFDFHLPEELIAQVPLEERETSRLMVLDRETGDIEHKHFTDILSYLHEGDCL
CCEEEEECEEECCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEE
VLNETKVMPARLHGVKEDTGAHIEVLLLKQEEGDKWETLVKPAKRVKEGTVISFGEGKLK
EEECCCCCHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCEEE
ATCTGTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLEDRDRYQTVYAKEIGSAA
EEEECCCCCCCCEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
APTAGLHFTEELLEKLKQKGVELAFITLHVGLGTFRPVSADTIEEHHMHAEYYHMSEETA
CCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHCHHHHHCCCHHHH
ALLNRVKENGGRIITVGTTSTRTLETIATDHNGKLCAASGWTDIFMYPGYEFKAIDGLIT
HHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCEEEECCCCEEEEECCCCEEHHHHHHH
NFHLPKSTLIMLVSAFANRDNVLHAYNEAVKEKYRFFSFGDAMFVASHAKMGNK
CCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHEEEHHHHCCCC
>Mature Secondary Structure
MDIVMDINLFDFHLPEELIAQVPLEERETSRLMVLDRETGDIEHKHFTDILSYLHEGDCL
CCEEEEECEEECCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEE
VLNETKVMPARLHGVKEDTGAHIEVLLLKQEEGDKWETLVKPAKRVKEGTVISFGEGKLK
EEECCCCCHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCEEE
ATCTGTADQGGRQLEFSYDGIFYEILDELGEMPLPPYIKETLEDRDRYQTVYAKEIGSAA
EEEECCCCCCCCEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
APTAGLHFTEELLEKLKQKGVELAFITLHVGLGTFRPVSADTIEEHHMHAEYYHMSEETA
CCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHCHHHHHCCCHHHH
ALLNRVKENGGRIITVGTTSTRTLETIATDHNGKLCAASGWTDIFMYPGYEFKAIDGLIT
HHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCEEEECCCCEEEEECCCCEEHHHHHHH
NFHLPKSTLIMLVSAFANRDNVLHAYNEAVKEKYRFFSFGDAMFVASHAKMGNK
CCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA