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The map label for this gene is cysM [H]

Identifier: 118479535

GI number: 118479535

Start: 4168695

End: 4169642

Strand: Reverse

Name: cysM [H]

Synonym: BALH_3958

Alternate gene names: 118479535

Gene position: 4169642-4168695 (Counterclockwise)

Preceding gene: 118479536

Following gene: 118479534

Centisome position: 79.31

GC content: 39.66

Gene sequence:

>948_bases
TTGGCAAATAAGGGGGAGAACACGATGAATGTATATCGTGGAGTTCATGAGTTAATTGGTCATACACCAATTGTGGAAAT
TACTCGTTTTTCACTTCCAGAAGGGGTCCGTTTATTTGCAAAGCTTGAATTTTATAATCCAGGCGGAAGCGTTAAGGACC
GGCTAGGAAGAGAATTAATAGAAGATGCGCTAGAAAAAGGGCTTGTTACAGAGGGCGGAACAATTATTGAACCGACTGCT
GGAAATACTGGTATTGGACTGGCACTTGCAGCGTTACAATATGATTTACGCGTTATTGTTTGTGTACCAGAGAAATTTAG
TATTGAAAAACAAGAATTAATGAAAGCACTAGGTGCAACGGTCGTGCATACACCGACTGAGCAAGGGATGACCGGTGCAA
TTGCAAAAGCGAAAGAGTTAGTAAATGAAATACCGAATTCATACTCTCCAAGTCAGTTTGCGAATGAAGCAAACCCTCGT
GCTTATTTCAAAACACTAGGTCCTGAACTTTGGTCGGCACTTAACGGAGAGATTAACATATTTGTTGCAGGAGCAGGAAC
TGGCGGAACATTTATGGGGACTGCATCTTATTTGAAAGAAAAAAATATAGATATAAAAACAGTTATTGTGGAACCAGAAG
GATCCATTTTAAATGGTGGTAAAGCTGGCTCACATGAAACAGAAGGGATTGGCCTTGAATTCATCCCGCCATTTTTGAAA
ACATCTTATTTTGATGAGATTCATACAATTTCTGATCGAAATGCATTTTTACGAGTGAAAGAATTAGCACAAAAAGAAGG
ATTGCTCGTTGGGAGCTCTTCAGGAGCAGCGTTTCATGCAAGTTTACTTGAAGCAGAGAAAGCGGCGCCAGGTACAAATA
TTGTAACGATTTTTCCTGATAGTAGTGAGCGCTATTTAAGTAAAGACATATACAAAGGATGGGAATAA

Upstream 100 bases:

>100_bases
TCTACAAACTTTATTGTAAAAGTATTAGAAGAGTTAAAGTAATGTAACAAAAAGGCAACTGTCTCATATAAAGAAAATAC
ATACAGTTGCCTTTTCTTTA

Downstream 100 bases:

>100_bases
AAAATGAGAGCAAAGACAAAGTTAATTCATGGTATTCGCATAGGAGAACCTTCAACTGGATCTGTAAATGTACCGATTTA
TCAAACGAGTACGTACAAAC

Product: cystathionine beta-synthase (acetylserine-dependent)

Products: L-cysteine; acetate

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MANKGENTMNVYRGVHELIGHTPIVEITRFSLPEGVRLFAKLEFYNPGGSVKDRLGRELIEDALEKGLVTEGGTIIEPTA
GNTGIGLALAALQYDLRVIVCVPEKFSIEKQELMKALGATVVHTPTEQGMTGAIAKAKELVNEIPNSYSPSQFANEANPR
AYFKTLGPELWSALNGEINIFVAGAGTGGTFMGTASYLKEKNIDIKTVIVEPEGSILNGGKAGSHETEGIGLEFIPPFLK
TSYFDEIHTISDRNAFLRVKELAQKEGLLVGSSSGAAFHASLLEAEKAAPGTNIVTIFPDSSERYLSKDIYKGWE

Sequences:

>Translated_315_residues
MANKGENTMNVYRGVHELIGHTPIVEITRFSLPEGVRLFAKLEFYNPGGSVKDRLGRELIEDALEKGLVTEGGTIIEPTA
GNTGIGLALAALQYDLRVIVCVPEKFSIEKQELMKALGATVVHTPTEQGMTGAIAKAKELVNEIPNSYSPSQFANEANPR
AYFKTLGPELWSALNGEINIFVAGAGTGGTFMGTASYLKEKNIDIKTVIVEPEGSILNGGKAGSHETEGIGLEFIPPFLK
TSYFDEIHTISDRNAFLRVKELAQKEGLLVGSSSGAAFHASLLEAEKAAPGTNIVTIFPDSSERYLSKDIYKGWE
>Mature_314_residues
ANKGENTMNVYRGVHELIGHTPIVEITRFSLPEGVRLFAKLEFYNPGGSVKDRLGRELIEDALEKGLVTEGGTIIEPTAG
NTGIGLALAALQYDLRVIVCVPEKFSIEKQELMKALGATVVHTPTEQGMTGAIAKAKELVNEIPNSYSPSQFANEANPRA
YFKTLGPELWSALNGEINIFVAGAGTGGTFMGTASYLKEKNIDIKTVIVEPEGSILNGGKAGSHETEGIGLEFIPPFLKT
SYFDEIHTISDRNAFLRVKELAQKEGLLVGSSSGAAFHASLLEAEKAAPGTNIVTIFPDSSERYLSKDIYKGWE

Specific function: Catalyzes the conversion of O-acetylserine and homocysteine to cystathionine [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=307, Percent_Identity=41.3680781758958, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI295821200, Length=307, Percent_Identity=41.3680781758958, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI4557415, Length=307, Percent_Identity=41.3680781758958, Blast_Score=225, Evalue=5e-59,
Organism=Escherichia coli, GI1788754, Length=304, Percent_Identity=41.1184210526316, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI2367138, Length=298, Percent_Identity=35.9060402684564, Blast_Score=177, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI17535051, Length=298, Percent_Identity=39.9328859060403, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI115535073, Length=299, Percent_Identity=42.4749163879599, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17562970, Length=298, Percent_Identity=35.9060402684564, Blast_Score=193, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI32566674, Length=293, Percent_Identity=36.518771331058, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17534315, Length=320, Percent_Identity=36.875, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17561720, Length=298, Percent_Identity=34.2281879194631, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25147552, Length=319, Percent_Identity=36.3636363636364, Blast_Score=165, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI32566672, Length=212, Percent_Identity=36.3207547169811, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71996324, Length=333, Percent_Identity=28.2282282282282, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321594, Length=332, Percent_Identity=38.855421686747, Blast_Score=209, Evalue=7e-55,
Organism=Saccharomyces cerevisiae, GI6321449, Length=326, Percent_Identity=35.5828220858896, Blast_Score=149, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24643623, Length=331, Percent_Identity=37.7643504531722, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI20129101, Length=331, Percent_Identity=37.7643504531722, Blast_Score=202, Evalue=2e-52,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 2.5.1.47

Molecular weight: Translated: 33978; Mature: 33847

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKGENTMNVYRGVHELIGHTPIVEITRFSLPEGVRLFAKLEFYNPGGSVKDRLGRELI
CCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEEECCCCHHHHHHHHHHH
EDALEKGLVTEGGTIIEPTAGNTGIGLALAALQYDLRVIVCVPEKFSIEKQELMKALGAT
HHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHCCE
VVHTPTEQGMTGAIAKAKELVNEIPNSYSPSQFANEANPRAYFKTLGPELWSALNGEINI
EEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHCHHHHHHCCCCEEE
FVAGAGTGGTFMGTASYLKEKNIDIKTVIVEPEGSILNGGKAGSHETEGIGLEFIPPFLK
EEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCEECCHHHH
TSYFDEIHTISDRNAFLRVKELAQKEGLLVGSSSGAAFHASLLEAEKAAPGTNIVTIFPD
HHHHHHHHHCCCCHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECC
SSERYLSKDIYKGWE
CCHHHHHHHHHCCCC
>Mature Secondary Structure 
ANKGENTMNVYRGVHELIGHTPIVEITRFSLPEGVRLFAKLEFYNPGGSVKDRLGRELI
CCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEEECCCCHHHHHHHHHHH
EDALEKGLVTEGGTIIEPTAGNTGIGLALAALQYDLRVIVCVPEKFSIEKQELMKALGAT
HHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHCCE
VVHTPTEQGMTGAIAKAKELVNEIPNSYSPSQFANEANPRAYFKTLGPELWSALNGEINI
EEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHCHHHHHHCCCCEEE
FVAGAGTGGTFMGTASYLKEKNIDIKTVIVEPEGSILNGGKAGSHETEGIGLEFIPPFLK
EEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCEECCHHHH
TSYFDEIHTISDRNAFLRVKELAQKEGLLVGSSSGAAFHASLLEAEKAAPGTNIVTIFPD
HHHHHHHHHCCCCHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECC
SSERYLSKDIYKGWE
CCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 44000 [C]

Specific activity: 1100

Km value (mM): 0.7 {L-Ser}} 0.12 {acetyl-CoA}} 3.6 {S2-}} 0.1 {S2-}} 20 {O-acetyl-L-Ser}} 7.1 {O-acetyl-L-Ser}} 5 {O-acetyl-L-Ser}} [C]

Substrates: O3-acetyl-L-serine; hydrogen sulfide

Specific reaction: O3-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate

General reaction: Elimination; C-O bond cleavage [C]

Inhibitor: acetyl-CoA; D-cycloserine; L-cysteine; NH2OH; semicarbazide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]