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The map label for this gene is aacA-aphD [H]

Identifier: 118478287

GI number: 118478287

Start: 2809817

End: 2810350

Strand: Reverse

Name: aacA-aphD [H]

Synonym: BALH_2648

Alternate gene names: 118478287

Gene position: 2810350-2809817 (Counterclockwise)

Preceding gene: 118478289

Following gene: 118478286

Centisome position: 53.46

GC content: 35.02

Gene sequence:

>534_bases
ATGCTATTTCAAAAAGATAATGTATCGGTTAGGTATGTAGTAGAAGAGGATGCACCAATCATTTCAAAATGGTTAACAGA
CCCAGAAGTACTGCAGTATTACGAAGGACGAGATGATCCGCAATCAGTAGAAATGGTACTGAATCATTTTATACATAATC
CAAATAGTCCTGAAAAAAGGTGCTTAATAGAGTTTGATGATGTGCCAATTGGTTATATTCAAATGTATCCAGTTGATTCG
GAGTGGAAAACATTATATGGCTATGAAGAATCGCAAAATGTATGGGGGATGGATCAATTTATTGGAGAACCGACTTATTG
GGGAAAAGGAATTGGAACAAAATTTGTTAAGGCTGCAATTACATACATTTTCAGTGAAATGGGAGCAGAAGCAATCGCAA
TGGACCCGAAAGTAAATAATGAACGTGCAATAAAGTGTTATGAAAGATGCGGATTTAAAAAGGTGAAGATTTTAAAGGAA
CATGAACTACATGAAGGGGTATTAGAAGATTGTTGGATGATGGAATATAAATAA

Upstream 100 bases:

>100_bases
ACAGCATTTTTTGCCATTACATTGTTACCATACGCACTAATAGGTGCAATGAAAGATTTCACTACAAGAAAAAATATAAA
GAATTAAAAAGAGGTACTAT

Downstream 100 bases:

>100_bases
AAGGGAGCGGCGCGATATGAAAAAAGCATTATTAGTAATTGATGTGCAAGCAGGTATGTATACAGCAGGAATGCCAGTAC
ATAATGGGGAAAAGTTTTTA

Product: acetyltransferase

Products: Coenzyme A; H+; N8-Acetylspermidine [C]

Alternate protein names: 6'-aminoglycoside N-acetyltransferase; AAC(6'); 2''-aminoglycoside phosphotransferase; APH(2'') [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MLFQKDNVSVRYVVEEDAPIISKWLTDPEVLQYYEGRDDPQSVEMVLNHFIHNPNSPEKRCLIEFDDVPIGYIQMYPVDS
EWKTLYGYEESQNVWGMDQFIGEPTYWGKGIGTKFVKAAITYIFSEMGAEAIAMDPKVNNERAIKCYERCGFKKVKILKE
HELHEGVLEDCWMMEYK

Sequences:

>Translated_177_residues
MLFQKDNVSVRYVVEEDAPIISKWLTDPEVLQYYEGRDDPQSVEMVLNHFIHNPNSPEKRCLIEFDDVPIGYIQMYPVDS
EWKTLYGYEESQNVWGMDQFIGEPTYWGKGIGTKFVKAAITYIFSEMGAEAIAMDPKVNNERAIKCYERCGFKKVKILKE
HELHEGVLEDCWMMEYK
>Mature_177_residues
MLFQKDNVSVRYVVEEDAPIISKWLTDPEVLQYYEGRDDPQSVEMVLNHFIHNPNSPEKRCLIEFDDVPIGYIQMYPVDS
EWKTLYGYEESQNVWGMDQFIGEPTYWGKGIGTKFVKAAITYIFSEMGAEAIAMDPKVNNERAIKCYERCGFKKVKILKE
HELHEGVLEDCWMMEYK

Specific function: Resistance to gentamicin, tobramycin, and kanamycin. Tobramycin and kanamycin resistance is due to the ACC activity, specified by N-terminal region, and the gentamicin resistance is due to the APH activity encoded by the C-terminal region of the protein [

COG id: COG1670

COG function: function code J; Acetyltransferases, including N-acetylases of ribosomal proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR002575
- InterPro:   IPR011009 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF01636 APH [H]

EC number: 2.3.1.57 [C]

Molecular weight: Translated: 20768; Mature: 20768

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFQKDNVSVRYVVEEDAPIISKWLTDPEVLQYYEGRDDPQSVEMVLNHFIHNPNSPEKR
CCCCCCCCEEEEEECCCCCHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCE
CLIEFDDVPIGYIQMYPVDSEWKTLYGYEESQNVWGMDQFIGEPTYWGKGIGTKFVKAAI
EEEEECCCCCCEEEEEECCCCCCEEECCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHH
TYIFSEMGAEAIAMDPKVNNERAIKCYERCGFKKVKILKEHELHEGVLEDCWMMEYK
HHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLFQKDNVSVRYVVEEDAPIISKWLTDPEVLQYYEGRDDPQSVEMVLNHFIHNPNSPEKR
CCCCCCCCEEEEEECCCCCHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCE
CLIEFDDVPIGYIQMYPVDSEWKTLYGYEESQNVWGMDQFIGEPTYWGKGIGTKFVKAAI
EEEEECCCCCCEEEEEECCCCCCEEECCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHH
TYIFSEMGAEAIAMDPKVNNERAIKCYERCGFKKVKILKEHELHEGVLEDCWMMEYK
HHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acetyl-CoA; Spermidine [C]

Specific reaction: Acetyl-CoA + Spermidine --> Coenzyme A + H+ + N8-Acetylspermidine [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3015884; 12663927 [H]