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The map label for this gene is acoA [H]

Identifier: 118478137

GI number: 118478137

Start: 2657913

End: 2658938

Strand: Reverse

Name: acoA [H]

Synonym: BALH_2494

Alternate gene names: 118478137

Gene position: 2658938-2657913 (Counterclockwise)

Preceding gene: 118478138

Following gene: 118478136

Centisome position: 50.58

GC content: 38.21

Gene sequence:

>1026_bases
ATGAACAAGAAAATAGGAGGGCTGGACATGTTAAAAACAACTGAAAGTAAAGGGAATGAAATTACGAAAGAACAAGCTCG
TTGGATGTATAAAAAAATGTTAGAGATTCGTAAATTTGAAGATAAGGTACATGAATTGTTCGCGCAAGGCATGCTGCCAG
GTTTCGTCCATTTATACGCAGGTGAAGAAGCGGTAGCAGTTGGTGTATGTGCTCACTTAACGGATAGTGACAGTATTACA
AGTACGCATAGAGGCCATGGGCATTGTATTGCAAAAGGTTGTGATTTAAACGGTATGATGGCTGAACTTTTTGGGAAAGC
GACAGGGTTATGTAAAGGTAAAGGCGGCTCCATGCATATTGCCGATTTAGATAAAGGAATGCTTGGTGCAAATGGCATTG
TAGGTGGTGGTTTCCCGCTTGCTTGCGGCTCGGCATTAACAGCTAAATATAAAGGAACAAAAGATGTAAGTGTTTGTTTC
TTTGGTGACGGAGCAAATAATGAAGGTACGTTTCATGAAGGGGTTAACTTAGCGGCGATTTGGAAGTTACCAGTTATTTT
TATCGCTGAAAATAACGGTTACGGTGAAGCAACGACATTTGAATATGCTTCAAGTTGTGATTCTATTGCGGATCGTGCGA
AAGCTTATAACATTCCAGGTGTTCAAGTGGATGGAAAAGATCTGCTAGCTGTATATAAAGCTGCTGAAGAAGCAGTAGAA
CGTGCGCGTAATGGCGATGGTCCAACAATAATTGAATGTATGACATATCGTAATTACGGTCATTTCGAAGGTGAAGCACA
AACGTATAAAACTTCAGAAGAAAAAGAAGAACATTTAAACGAAAAAGATGCAATTGTGAATTTCCGTAAGCATTTAATTC
ATGAAGCTTTATTAACTGAGTCTGAACTTGTGGATATGGAAAAGGCAGTAGATGAGGCAGTACAAAGATCAATTGAATTT
AGTGAAAATAGTCCATATCCAGAGGATGAAGAATTATTAAAAGATGTTTATGTTTCTTATAAATAA

Upstream 100 bases:

>100_bases
AGACTAATGTCTCATTTTGTCCACTTTTTTATTTTCAACATAGAGAGAAAACCTATTAAATTAAGGATTAGAATTTGGCA
CGGTACTTGCAATATAAAAG

Downstream 100 bases:

>100_bases
GAGGAGGGTGAAAAATTATGACTAGAACAGTAAGTATGTCAACTGCAATCAATGAAGCGATGAAAATCTCAATGCGCCGT
GATGAAAATGTAATTTTAAT

Product: acetoin dehydrogenase (TPP-dependent) E1 component subunit alpha

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNKKIGGLDMLKTTESKGNEITKEQARWMYKKMLEIRKFEDKVHELFAQGMLPGFVHLYAGEEAVAVGVCAHLTDSDSIT
STHRGHGHCIAKGCDLNGMMAELFGKATGLCKGKGGSMHIADLDKGMLGANGIVGGGFPLACGSALTAKYKGTKDVSVCF
FGDGANNEGTFHEGVNLAAIWKLPVIFIAENNGYGEATTFEYASSCDSIADRAKAYNIPGVQVDGKDLLAVYKAAEEAVE
RARNGDGPTIIECMTYRNYGHFEGEAQTYKTSEEKEEHLNEKDAIVNFRKHLIHEALLTESELVDMEKAVDEAVQRSIEF
SENSPYPEDEELLKDVYVSYK

Sequences:

>Translated_341_residues
MNKKIGGLDMLKTTESKGNEITKEQARWMYKKMLEIRKFEDKVHELFAQGMLPGFVHLYAGEEAVAVGVCAHLTDSDSIT
STHRGHGHCIAKGCDLNGMMAELFGKATGLCKGKGGSMHIADLDKGMLGANGIVGGGFPLACGSALTAKYKGTKDVSVCF
FGDGANNEGTFHEGVNLAAIWKLPVIFIAENNGYGEATTFEYASSCDSIADRAKAYNIPGVQVDGKDLLAVYKAAEEAVE
RARNGDGPTIIECMTYRNYGHFEGEAQTYKTSEEKEEHLNEKDAIVNFRKHLIHEALLTESELVDMEKAVDEAVQRSIEF
SENSPYPEDEELLKDVYVSYK
>Mature_341_residues
MNKKIGGLDMLKTTESKGNEITKEQARWMYKKMLEIRKFEDKVHELFAQGMLPGFVHLYAGEEAVAVGVCAHLTDSDSIT
STHRGHGHCIAKGCDLNGMMAELFGKATGLCKGKGGSMHIADLDKGMLGANGIVGGGFPLACGSALTAKYKGTKDVSVCF
FGDGANNEGTFHEGVNLAAIWKLPVIFIAENNGYGEATTFEYASSCDSIADRAKAYNIPGVQVDGKDLLAVYKAAEEAVE
RARNGDGPTIIECMTYRNYGHFEGEAQTYKTSEEKEEHLNEKDAIVNFRKHLIHEALLTESELVDMEKAVDEAVQRSIEF
SENSPYPEDEELLKDVYVSYK

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=320, Percent_Identity=37.8125, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI291084742, Length=320, Percent_Identity=37.1875, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI4505685, Length=320, Percent_Identity=37.1875, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI291084744, Length=327, Percent_Identity=36.3914373088685, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI291084757, Length=320, Percent_Identity=32.8125, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI11386135, Length=343, Percent_Identity=29.1545189504373, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI258645172, Length=343, Percent_Identity=29.1545189504373, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17536047, Length=318, Percent_Identity=38.3647798742138, Blast_Score=222, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI32564172, Length=318, Percent_Identity=38.3647798742138, Blast_Score=222, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI86563355, Length=341, Percent_Identity=29.9120234604106, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI86563357, Length=341, Percent_Identity=29.9120234604106, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321026, Length=322, Percent_Identity=37.2670807453416, Blast_Score=216, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24639744, Length=335, Percent_Identity=39.1044776119403, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI28571106, Length=335, Percent_Identity=39.1044776119403, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24639740, Length=335, Percent_Identity=39.1044776119403, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24639748, Length=319, Percent_Identity=38.871473354232, Blast_Score=207, Evalue=9e-54,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=39.2156862745098, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI21355903, Length=326, Percent_Identity=26.6871165644172, Blast_Score=124, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 37318; Mature: 37318

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKIGGLDMLKTTESKGNEITKEQARWMYKKMLEIRKFEDKVHELFAQGMLPGFVHLYA
CCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
GEEAVAVGVCAHLTDSDSITSTHRGHGHCIAKGCDLNGMMAELFGKATGLCKGKGGSMHI
CCCEEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCCEEE
ADLDKGMLGANGIVGGGFPLACGSALTAKYKGTKDVSVCFFGDGANNEGTFHEGVNLAAI
EECCCCCCCCCCCCCCCCHHHCCCHHEECCCCCCCEEEEEEECCCCCCCCHHCCCCEEEE
WKLPVIFIAENNGYGEATTFEYASSCDSIADRAKAYNIPGVQVDGKDLLAVYKAAEEAVE
EECCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHH
RARNGDGPTIIECMTYRNYGHFEGEAQTYKTSEEKEEHLNEKDAIVNFRKHLIHEALLTE
HHHCCCCCEEEEEEEECCCCCCCCCCHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SELVDMEKAVDEAVQRSIEFSENSPYPEDEELLKDVYVSYK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNKKIGGLDMLKTTESKGNEITKEQARWMYKKMLEIRKFEDKVHELFAQGMLPGFVHLYA
CCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
GEEAVAVGVCAHLTDSDSITSTHRGHGHCIAKGCDLNGMMAELFGKATGLCKGKGGSMHI
CCCEEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCCEEE
ADLDKGMLGANGIVGGGFPLACGSALTAKYKGTKDVSVCFFGDGANNEGTFHEGVNLAAI
EECCCCCCCCCCCCCCCCHHHCCCHHEECCCCCCCEEEEEEECCCCCCCCHHCCCCEEEE
WKLPVIFIAENNGYGEATTFEYASSCDSIADRAKAYNIPGVQVDGKDLLAVYKAAEEAVE
EECCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHH
RARNGDGPTIIECMTYRNYGHFEGEAQTYKTSEEKEEHLNEKDAIVNFRKHLIHEALLTE
HHHCCCCCEEEEEEEECCCCCCCCCCHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SELVDMEKAVDEAVQRSIEFSENSPYPEDEELLKDVYVSYK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]