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The map label for this gene is iolD1 [H]

Identifier: 118477915

GI number: 118477915

Start: 2423069

End: 2425003

Strand: Direct

Name: iolD1 [H]

Synonym: BALH_2260

Alternate gene names: 118477915

Gene position: 2423069-2425003 (Clockwise)

Preceding gene: 118477914

Following gene: 118477916

Centisome position: 46.09

GC content: 38.97

Gene sequence:

>1935_bases
ATGCAAACTGTTAGAATGACGACGGCGCAAGCATTGGTGAAATTTTTGAATCAGCAATACGTAGAGTTTGATGGAAAGCA
ACAAAAGTTTATTAAGGGAATCTTTACGATTTTTGGTCATGGGAATGTAGTAGGCCTAGGTCAAGCTTTAGAAGAAGATG
CAGGGGAATTAGAAGTATATCAAGGGAGAAATGAACAAGGAATGGCAAATGCTGCGATGGCTTTTGCAAAACAAAAACAT
AGAAAACAAATTATGGCATGTACTTCTTCTGTTGGTCCTGGATCAGCAAATATGATTACCTCTGCAGCAACAGCTTCTGC
AAATAATATTCCCGTTTTATTACTTCCAGGAGATGTATTTGCAACGAGACAATCCGATCCTGTTCTTCAACAAATTGAAC
AAACACATGACTTATCTATTTCTACAAATGATGCTTTCCGTGCAGTAAGTAAGTACTGGGACAGGATAAATCGTCCTGAA
CAATTGATGACAGCTATGATTCAAGCGATGCGTGTTTTAACGAATCCGGCAGATACAGGGGCTGTAACAATTTGTTTACC
ACAAGATGTCCAGGGGGAAGCATGGGATTTTCCAGATTACTTCTTCCAAAAGCGTGTTCACCGTATTGAACGTCGTCTAC
CTACAAAAGCCAGCTTAGCGGATGCAGTGGAAATGATTAAGAGAAAGAAAAAGCCAGTTATGATTTGTGGTGGTGGTGTA
AGATACGCAGAAGCGGCAGAAGAGCTAAAACAGTTTGCTGAAACATTCCACATTCCATTTGGAGAAACACAAGCTGGAAA
AAGTGCAATTGAAAGTAGTCATCCATATAATCTAGGCGGCATTGGGGTAACAGGAAATATAGCAGCTAATACAATTGCAA
AGGAAGCGGATCTTGTTATTGGAATCGGGACGAGATTTACTGATTTTACAACGGCATCGAAACAATTATTTCAAAATGAG
GAAGTTGAGTTTTTAAACATCAATATTTCAGAATTCCATGCGAATAAGCTGGATGCATTAAAGGTTATAGCGGATGCGAA
AGAAGCGCTTCTTGTTCTAATAGATGAACTGCAAGTAATGGATTATCGATCTAGTTATACAGTAGAAATTGCTGATGCAA
AAGAGGCTTGGGAAACAGAATTATCACGTCTACATAATATTCGCTTTACAGGTCAAGATTTCACACCAGAAGTCGAAGGT
CATTTCGATGGAAATTTAAATGAGTATGTAGACGCACTTGGTTCACAATTAACACAGACTGCAGTCATTGGACAAATCAA
TACATTACTTGATGAAGATGCAATTATCGTTGGAGCGGCAGGAAGTCTTCCAGGTGATTTACAAAGAATGTGGGCATCAA
GAAAACCAAATACATATCACATGGAGTATGGATACTCTTGCATGGGCTATGAGGTTGCGGGCGCACTAGGTGCAAAGCTA
GCTGAACCATCGAAGGAAGTATATGCAATGGTAGGGGATGGTAGTTACCAGATGCTTCATTCTGAGCTTGTCACAAGTCT
TCAAGAAAACAAAAAAATTAACGTCTTATTGTTTGATAATTCCGGATTTGGTTGCATTAATAACTTACAAATGGGCAATG
GAATGGGGAGCTTTGGAACAGAGTTTCGCTATCGAAACCAGGAAACTCGTAAATTAGACGGGACAATTATGAAAATTGAT
TTTGCAGCTAGCGCAGCTGGATATGGTGTGAAAACATATCATGTTACATCACTAGAACAATTACAGGAAGCACTTATAGA
TGCGAAAAAACAAACAGTTTCTACATTAATTGATATTAAAGTACTGCCAAAAACAATGACAAATGGATACGAGTCATGGT
GGCATGTAGGTATTGCAGAAGTATCTAAAAGTCAAAGGGTACAAGCTGCGTACGAGAGTAAAGTAAGTAACTTGCAACAG
GCAAGGTCTTATTAG

Upstream 100 bases:

>100_bases
GTAGTAAAGCATAAGAAGGAAGTATTAGGAGTCTATCAAATTCCTAATACTCCTTTTTCATATGCAGAATGACTGTTAGA
GATAGAAAGGAGCAAATCAT

Downstream 100 bases:

>100_bases
ATTAGGGTACAAAGAGAGCTTTTTATCTACATGCATATGGTTTAAGAAGCTCTCTTTTTAAAGAATAGATAAGAGAAGTT
GGCGAAATTCTATCACATTT

Product: malonic semialdehyde oxidative decarboxylase

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase 1 [H]

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MQTVRMTTAQALVKFLNQQYVEFDGKQQKFIKGIFTIFGHGNVVGLGQALEEDAGELEVYQGRNEQGMANAAMAFAKQKH
RKQIMACTSSVGPGSANMITSAATASANNIPVLLLPGDVFATRQSDPVLQQIEQTHDLSISTNDAFRAVSKYWDRINRPE
QLMTAMIQAMRVLTNPADTGAVTICLPQDVQGEAWDFPDYFFQKRVHRIERRLPTKASLADAVEMIKRKKKPVMICGGGV
RYAEAAEELKQFAETFHIPFGETQAGKSAIESSHPYNLGGIGVTGNIAANTIAKEADLVIGIGTRFTDFTTASKQLFQNE
EVEFLNINISEFHANKLDALKVIADAKEALLVLIDELQVMDYRSSYTVEIADAKEAWETELSRLHNIRFTGQDFTPEVEG
HFDGNLNEYVDALGSQLTQTAVIGQINTLLDEDAIIVGAAGSLPGDLQRMWASRKPNTYHMEYGYSCMGYEVAGALGAKL
AEPSKEVYAMVGDGSYQMLHSELVTSLQENKKINVLLFDNSGFGCINNLQMGNGMGSFGTEFRYRNQETRKLDGTIMKID
FAASAAGYGVKTYHVTSLEQLQEALIDAKKQTVSTLIDIKVLPKTMTNGYESWWHVGIAEVSKSQRVQAAYESKVSNLQQ
ARSY

Sequences:

>Translated_644_residues
MQTVRMTTAQALVKFLNQQYVEFDGKQQKFIKGIFTIFGHGNVVGLGQALEEDAGELEVYQGRNEQGMANAAMAFAKQKH
RKQIMACTSSVGPGSANMITSAATASANNIPVLLLPGDVFATRQSDPVLQQIEQTHDLSISTNDAFRAVSKYWDRINRPE
QLMTAMIQAMRVLTNPADTGAVTICLPQDVQGEAWDFPDYFFQKRVHRIERRLPTKASLADAVEMIKRKKKPVMICGGGV
RYAEAAEELKQFAETFHIPFGETQAGKSAIESSHPYNLGGIGVTGNIAANTIAKEADLVIGIGTRFTDFTTASKQLFQNE
EVEFLNINISEFHANKLDALKVIADAKEALLVLIDELQVMDYRSSYTVEIADAKEAWETELSRLHNIRFTGQDFTPEVEG
HFDGNLNEYVDALGSQLTQTAVIGQINTLLDEDAIIVGAAGSLPGDLQRMWASRKPNTYHMEYGYSCMGYEVAGALGAKL
AEPSKEVYAMVGDGSYQMLHSELVTSLQENKKINVLLFDNSGFGCINNLQMGNGMGSFGTEFRYRNQETRKLDGTIMKID
FAASAAGYGVKTYHVTSLEQLQEALIDAKKQTVSTLIDIKVLPKTMTNGYESWWHVGIAEVSKSQRVQAAYESKVSNLQQ
ARSY
>Mature_644_residues
MQTVRMTTAQALVKFLNQQYVEFDGKQQKFIKGIFTIFGHGNVVGLGQALEEDAGELEVYQGRNEQGMANAAMAFAKQKH
RKQIMACTSSVGPGSANMITSAATASANNIPVLLLPGDVFATRQSDPVLQQIEQTHDLSISTNDAFRAVSKYWDRINRPE
QLMTAMIQAMRVLTNPADTGAVTICLPQDVQGEAWDFPDYFFQKRVHRIERRLPTKASLADAVEMIKRKKKPVMICGGGV
RYAEAAEELKQFAETFHIPFGETQAGKSAIESSHPYNLGGIGVTGNIAANTIAKEADLVIGIGTRFTDFTTASKQLFQNE
EVEFLNINISEFHANKLDALKVIADAKEALLVLIDELQVMDYRSSYTVEIADAKEAWETELSRLHNIRFTGQDFTPEVEG
HFDGNLNEYVDALGSQLTQTAVIGQINTLLDEDAIIVGAAGSLPGDLQRMWASRKPNTYHMEYGYSCMGYEVAGALGAKL
AEPSKEVYAMVGDGSYQMLHSELVTSLQENKKINVLLFDNSGFGCINNLQMGNGMGSFGTEFRYRNQETRKLDGTIMKID
FAASAAGYGVKTYHVTSLEQLQEALIDAKKQTVSTLIDIKVLPKTMTNGYESWWHVGIAEVSKSQRVQAAYESKVSNLQQ
ARSY

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=20.4262877442274, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1790104, Length=607, Percent_Identity=24.0527182866557, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1786717, Length=567, Percent_Identity=25.5731922398589, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI87081685, Length=584, Percent_Identity=24.8287671232877, Blast_Score=126, Evalue=5e-30,
Organism=Escherichia coli, GI1787096, Length=527, Percent_Identity=22.2011385199241, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI1788716, Length=532, Percent_Identity=21.2406015037594, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17531299, Length=441, Percent_Identity=24.0362811791383, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17531301, Length=441, Percent_Identity=24.0362811791383, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323755, Length=591, Percent_Identity=23.3502538071066, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922626, Length=629, Percent_Identity=22.5755166931638, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 71074; Mature: 71074

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTVRMTTAQALVKFLNQQYVEFDGKQQKFIKGIFTIFGHGNVVGLGQALEEDAGELEVY
CCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEECCHHHHCCCCCEEEE
QGRNEQGMANAAMAFAKQKHRKQIMACTSSVGPGSANMITSAATASANNIPVLLLPGDVF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEHHHCCCCCCCEEEEECCCEE
ATRQSDPVLQQIEQTHDLSISTNDAFRAVSKYWDRINRPEQLMTAMIQAMRVLTNPADTG
ECCCCCHHHHHHHHHHCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AVTICLPQDVQGEAWDFPDYFFQKRVHRIERRLPTKASLADAVEMIKRKKKPVMICGGGV
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCC
RYAEAAEELKQFAETFHIPFGETQAGKSAIESSHPYNLGGIGVTGNIAANTIAKEADLVI
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCEECCHHHHHHHCCCCEEE
GIGTRFTDFTTASKQLFQNEEVEFLNINISEFHANKLDALKVIADAKEALLVLIDELQVM
ECCCCCCCHHHHHHHHHCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DYRSSYTVEIADAKEAWETELSRLHNIRFTGQDFTPEVEGHFDGNLNEYVDALGSQLTQT
CCCCCEEEEECCCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVIGQINTLLDEDAIIVGAAGSLPGDLQRMWASRKPNTYHMEYGYSCMGYEVAGALGAKL
HHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCCEEECHHHHHHHHHHH
AEPSKEVYAMVGDGSYQMLHSELVTSLQENKKINVLLFDNSGFGCINNLQMGNGMGSFGT
CCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCCCCCCCC
EFRYRNQETRKLDGTIMKIDFAASAAGYGVKTYHVTSLEQLQEALIDAKKQTVSTLIDIK
CCEECCCHHHHHCCEEEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHE
VLPKTMTNGYESWWHVGIAEVSKSQRVQAAYESKVSNLQQARSY
EECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTVRMTTAQALVKFLNQQYVEFDGKQQKFIKGIFTIFGHGNVVGLGQALEEDAGELEVY
CCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEECCHHHHCCCCCEEEE
QGRNEQGMANAAMAFAKQKHRKQIMACTSSVGPGSANMITSAATASANNIPVLLLPGDVF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEHHHCCCCCCCEEEEECCCEE
ATRQSDPVLQQIEQTHDLSISTNDAFRAVSKYWDRINRPEQLMTAMIQAMRVLTNPADTG
ECCCCCHHHHHHHHHHCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AVTICLPQDVQGEAWDFPDYFFQKRVHRIERRLPTKASLADAVEMIKRKKKPVMICGGGV
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCC
RYAEAAEELKQFAETFHIPFGETQAGKSAIESSHPYNLGGIGVTGNIAANTIAKEADLVI
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCEECCHHHHHHHCCCCEEE
GIGTRFTDFTTASKQLFQNEEVEFLNINISEFHANKLDALKVIADAKEALLVLIDELQVM
ECCCCCCCHHHHHHHHHCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DYRSSYTVEIADAKEAWETELSRLHNIRFTGQDFTPEVEGHFDGNLNEYVDALGSQLTQT
CCCCCEEEEECCCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVIGQINTLLDEDAIIVGAAGSLPGDLQRMWASRKPNTYHMEYGYSCMGYEVAGALGAKL
HHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCCEEECHHHHHHHHHHH
AEPSKEVYAMVGDGSYQMLHSELVTSLQENKKINVLLFDNSGFGCINNLQMGNGMGSFGT
CCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCCCCCCCC
EFRYRNQETRKLDGTIMKIDFAASAAGYGVKTYHVTSLEQLQEALIDAKKQTVSTLIDIK
CCEECCCHHHHHCCEEEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHE
VLPKTMTNGYESWWHVGIAEVSKSQRVQAAYESKVSNLQQARSY
EECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA