Click here to switch to the map view.

The map label for this gene is dfrD [H]

Identifier: 118477669

GI number: 118477669

Start: 2151650

End: 2152207

Strand: Direct

Name: dfrD [H]

Synonym: BALH_2001

Alternate gene names: 118477669

Gene position: 2151650-2152207 (Clockwise)

Preceding gene: 118477668

Following gene: 118477670

Centisome position: 40.93

GC content: 34.95

Gene sequence:

>558_bases
TTGAAGGATATGATCCACATCCAGCAATTAAAGCACCAGTTGCAGTGTAATTGTAGAGGAGATGAAAAGATGATAGTTTC
ATTTATGGTCGCAATGGACGAGAATCGAGTAATTGGTAAAGATAATAATTTACCTTGGCGCTTACCAAGTGAATTGCAGT
ATGTGAAGAAAACAACGATGGGTCATCCGCTTATTATGGGGAGAAAGAATTATGAAGCGATTGGTAGACCACTGCCTGGA
AGACGTAATATTATTGTAACTCGCAATGGAGGGTATCATGTTGAAGGTTGTGAAGTAGCACATTCTGTAGAAGAAGTGTT
TGAACTATGCAAAAATGAAGAAGAGATCTTTATTTTTGGTGGAGCACAAATTTATGATCTCTTTTTACCTTACGTAGACA
AGTTATATATAACAAAAATCCATCATGCATTTGAAGGAGATACATTCTTCCCAGAAATGGATATGACAAATTGGAAAGAA
GTTTTTGTTGAAAAAGGTTTAACGGATGAAAAAAATCCGTATACGTATTATTACCATGTATATGAGAAACAACAATAA

Upstream 100 bases:

>100_bases
GTAGAAAAGCAATTGGCACGTGAACCACGTCCATTCCCGAAACTTACACTAAATCCAGATGTGAAATCTGTGTTTGATTT
TGAAATGGAAGATTTAACAA

Downstream 100 bases:

>100_bases
TCCCAAAGATTGATGTGAATATTCACATCAATCTTTTTATTTGTATCCATTAATATTGGTTTATAGATGAGATTCGATAG
TGCTATAATGGAAGGTAGCA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MKDMIHIQQLKHQLQCNCRGDEKMIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPG
RRNIIVTRNGGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKE
VFVEKGLTDEKNPYTYYYHVYEKQQ

Sequences:

>Translated_185_residues
MKDMIHIQQLKHQLQCNCRGDEKMIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPG
RRNIIVTRNGGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKE
VFVEKGLTDEKNPYTYYYHVYEKQQ
>Mature_185_residues
MKDMIHIQQLKHQLQCNCRGDEKMIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPG
RRNIIVTRNGGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKE
VFVEKGLTDEKNPYTYYYHVYEKQQ

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=178, Percent_Identity=32.0224719101124, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI28827793, Length=156, Percent_Identity=30.1282051282051, Blast_Score=68, Evalue=6e-12,
Organism=Escherichia coli, GI1786233, Length=160, Percent_Identity=39.375, Blast_Score=140, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17505899, Length=155, Percent_Identity=32.9032258064516, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324810, Length=171, Percent_Identity=30.4093567251462, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116008026, Length=177, Percent_Identity=32.2033898305085, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24647458, Length=177, Percent_Identity=32.2033898305085, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 21832; Mature: 21832

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDMIHIQQLKHQLQCNCRGDEKMIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTM
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHCC
GHPLIMGRKNYEAIGRPLPGRRNIIVTRNGGYHVEGCEVAHSVEEVFELCKNEEEIFIFG
CCCEEECCCCHHHHCCCCCCCCCEEEECCCCEEECHHHHHHHHHHHHHHHCCCCEEEEEC
GAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEE
YEKQQ
EECCC
>Mature Secondary Structure
MKDMIHIQQLKHQLQCNCRGDEKMIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTM
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHCC
GHPLIMGRKNYEAIGRPLPGRRNIIVTRNGGYHVEGCEVAHSVEEVFELCKNEEEIFIFG
CCCEEECCCCHHHHCCCCCCCCCEEEECCCCEEECHHHHHHHHHHHHHHHCCCCEEEEEC
GAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEE
YEKQQ
EECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8540692 [H]