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The map label for this gene is pheD [H]

Identifier: 118477517

GI number: 118477517

Start: 1994316

End: 1995089

Strand: Direct

Name: pheD [H]

Synonym: BALH_1842

Alternate gene names: 118477517

Gene position: 1994316-1995089 (Clockwise)

Preceding gene: 118477516

Following gene: 118477518

Centisome position: 37.94

GC content: 35.66

Gene sequence:

>774_bases
TTGTTAATGAAAGAATTAGCTGATGAACTATTACAGGCTGAAGATTCACGTGTAGGAATACCGCCTTTTACTGAGAGATA
TGCTAATTTATCTATTGAGGATGCATATAATATTCAACTTAAAGTAGTAGAAAGAAAGTTGGAGAAAGGGCGTTATGTAA
TCGGAAAGAAAGTTGGACTTACGAGTGTTGCGATGCAGCAGATGTTTGGGGTAAATGAACCAGATTATGGACATTTGTTA
GATGACATGAAAATAGAAAATGGAAGTACAATCTCAATAAGTTCTTTTGTAGCTCCGAAAATAGAAGCAGAAATAGGATT
TGTTTTAGAGAAAGATTTAAAAGGGCCTAATATTACATATATTGATGTACTGATGGCAACGAAGTATGTTGTACCTACAC
TTGAAATAATTGATAGTCGTATTTCCGATTGGAAAATAAAATTGGCCGATACAGTAGCGGATAATGGCTCGTCAGCAAAA
GTTGTAATTGGGAACACATTTTCTAGCATCGATAAAGTAGATTTACGTACAACTGGAATGACACTTTATAAAAATAATAG
TTTAATTGCAACGGGGGCTGGTGCTGCTGCATTAGGGCATCCTGCACAAGCTATTGCTTGGCTAGCTAATAAACTACATG
AATTTAATATTGAATTAAAAGCAGGAGAGTTAATTTTACCAGGTGCGTTATCTGGTGCAGTCTCAGTGAATAGTAAAGAT
AGCATTCAAGCTGACTTCGGTTCACTAGGATCGGTTTCAGTTACATTCATATAG

Upstream 100 bases:

>100_bases
AGCTCCCATCTGGTGTTTATAAAAATAAGTAGATGGGTGGGGGCTAGTCTACAAAGTAAAGAATTGAGGATATATAAATT
AAAAAATGAGGTGAGAGCAA

Downstream 100 bases:

>100_bases
GTTAAAATGCTATGAAAAGGTGGGATAGATGTGCAGAAAGTAAAGGCGGCCATAATTGGATCAGGTAATATTGGTACGGA
TTTAATGTATAAATTAAGAA

Product: 2-hydroxypenta-2,4-dienoate hydratase

Products: 2-oxopent-4-enoate; H2O

Alternate protein names: HPH; 2-oxopent-4-enoate hydratase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLMKELADELLQAEDSRVGIPPFTERYANLSIEDAYNIQLKVVERKLEKGRYVIGKKVGLTSVAMQQMFGVNEPDYGHLL
DDMKIENGSTISISSFVAPKIEAEIGFVLEKDLKGPNITYIDVLMATKYVVPTLEIIDSRISDWKIKLADTVADNGSSAK
VVIGNTFSSIDKVDLRTTGMTLYKNNSLIATGAGAAALGHPAQAIAWLANKLHEFNIELKAGELILPGALSGAVSVNSKD
SIQADFGSLGSVSVTFI

Sequences:

>Translated_257_residues
MLMKELADELLQAEDSRVGIPPFTERYANLSIEDAYNIQLKVVERKLEKGRYVIGKKVGLTSVAMQQMFGVNEPDYGHLL
DDMKIENGSTISISSFVAPKIEAEIGFVLEKDLKGPNITYIDVLMATKYVVPTLEIIDSRISDWKIKLADTVADNGSSAK
VVIGNTFSSIDKVDLRTTGMTLYKNNSLIATGAGAAALGHPAQAIAWLANKLHEFNIELKAGELILPGALSGAVSVNSKD
SIQADFGSLGSVSVTFI
>Mature_257_residues
MLMKELADELLQAEDSRVGIPPFTERYANLSIEDAYNIQLKVVERKLEKGRYVIGKKVGLTSVAMQQMFGVNEPDYGHLL
DDMKIENGSTISISSFVAPKIEAEIGFVLEKDLKGPNITYIDVLMATKYVVPTLEIIDSRISDWKIKLADTVADNGSSAK
VVIGNTFSSIDKVDLRTTGMTLYKNNSLIATGAGAAALGHPAQAIAWLANKLHEFNIELKAGELILPGALSGAVSVNSKD
SIQADFGSLGSVSVTFI

Specific function: Conversion of 2-hydroxypent-2,4-dienoate into 4-hydroxy- 2-oxopentanoate [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=254, Percent_Identity=38.9763779527559, Blast_Score=194, Evalue=5e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.80

Molecular weight: Translated: 27752; Mature: 27752

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMKELADELLQAEDSRVGIPPFTERYANLSIEDAYNIQLKVVERKLEKGRYVIGKKVGL
CCHHHHHHHHHHCCCCCCCCCCHHHHHHCCEECCCCEEEEHEEHHHHHCCCEEEECCCCH
TSVAMQQMFGVNEPDYGHLLDDMKIENGSTISISSFVAPKIEAEIGFVLEKDLKGPNITY
HHHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEHHHCCCCCCCEECEEEECCCCCCCEEE
IDVLMATKYVVPTLEIIDSRISDWKIKLADTVADNGSSAKVVIGNTFSSIDKVDLRTTGM
EHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEECCCCCCCCCEEEEECCE
TLYKNNSLIATGAGAAALGHPAQAIAWLANKLHEFNIELKAGELILPGALSGAVSVNSKD
EEEECCCEEEECCCCHHHCCHHHHHHHHHHHHHHEEEEEECCCEEECCCCCCEEECCCCC
SIQADFGSLGSVSVTFI
CCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MLMKELADELLQAEDSRVGIPPFTERYANLSIEDAYNIQLKVVERKLEKGRYVIGKKVGL
CCHHHHHHHHHHCCCCCCCCCCHHHHHHCCEECCCCEEEEHEEHHHHHCCCEEEECCCCH
TSVAMQQMFGVNEPDYGHLLDDMKIENGSTISISSFVAPKIEAEIGFVLEKDLKGPNITY
HHHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEHHHCCCCCCCEECEEEECCCCCCCEEE
IDVLMATKYVVPTLEIIDSRISDWKIKLADTVADNGSSAKVVIGNTFSSIDKVDLRTTGM
EHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEECCCCCCCCCEEEEECCE
TLYKNNSLIATGAGAAALGHPAQAIAWLANKLHEFNIELKAGELILPGALSGAVSVNSKD
EEEECCCEEEECCCCHHHCCHHHHHHHHHHHHHHEEEEEECCCEEECCCCCCEEECCCCC
SIQADFGSLGSVSVTFI
CCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxy-2-oxopentanoate

Specific reaction: 4-hydroxy-2-oxopentanoate = 2-oxopent-4-enoate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1791759 [H]