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The map label for this gene is brnQ [H]

Identifier: 118477371

GI number: 118477371

Start: 1843765

End: 1845141

Strand: Direct

Name: brnQ [H]

Synonym: BALH_1690

Alternate gene names: 118477371

Gene position: 1843765-1845141 (Clockwise)

Preceding gene: 229576701

Following gene: 118477372

Centisome position: 35.07

GC content: 35.95

Gene sequence:

>1377_bases
TTGAATGGAGGTTATATTATGACGAATAAAGTACCGTTTTCGTTCATAGTTGTTATTGGTTTAATGTTATTTGCACTATT
TTTTGGGGCAGGAAATTTAATATTCCCAGCAATGCTTGGTCAATCGGCAGGAGAGAATGTGTGGATTGCTAACGCTGGAT
TTTTAGTAACAGGTGTAGGTTTACCGTTGCTAGGGGTATTAGCATTTGGAATTTCAGGTAAAGAAGATTTACAATCGTTA
GCAAGTCGTGTACATCCATTGTTCGGTATTGTATTTACGACAGTTTTATATTTAGCGATTGGTCCACTATTCGCTATTCC
GAGAACAGGCAGCGTTTCTTATGAAATTGGTATGAAGCCTTTTGTACCAGAAGCTATGAGTTCTTCAGCACTCATTCTTT
TTACAATTATATTCTTTAGCATCACTTGTTTTTTTTCGCTAAACCCCGCGAAAATTGTCGATATTGTAGGAAAAATCTTA
ACGCCAATTAAGTTAACGTTTATTGGGATTTTAGTAGTATTTGCTTTTATTCATCCAATGGGAGAAATGCAAGCACCTGC
AGAGGCATATACATCACATGCGTTCTTTAAAGGGTTCCAAGAAGGTTACTTAACAATGGATACGTTAGCATCATTCGTAT
TCGGTATTATTATCATCAATGCGATTAAAGAAAAGGGCGCAAAAACAAAAAAAGACATTATGATTGTTTGTGCAAAAGCA
ACATTAATTGCTGCATCTATTTTAGCAATGATCTATTCAGCACTTTCATTTATGGGAGCTTCAAGTGTTGCAAAACTTGG
TCATTTAGATAATGGTGGTGCAGTATTAGCAAAAGTTTCAAACTATTATTTTGGGTCATACGGCGGAATTATACTAGGGA
TTATGGTTACAGCAGCATGTTTAACGACTAGTGTAGGACTTGTTTCAGCGTGTTCTTCATTCTTCCATAAACTATTCCCG
AATGTTTCGTATAAAAAGATAGCAATCATCTTATCAGTGTTCAGTGCAGTTGTTGCGAATGTAGGTTTAACGCAATTAAT
CGCAATTTCTGTTCCTGTATTAATCGCGATTTATCCATTAGCAATCGTCTTAATTTTCCTAACGCTCCTTCATCCATTGT
TTAAAGGACGAGCAGAAGTGTATCAAGGTAGCTTATTAATTACATTTATTGTAAGTGTGTTCGATGGATTGAATGCAGCT
GGTATGAAGTTAGCAGTAGTGAACGATTTTCTTACTAAAATACTCCCAATGCATGAAGTTGGATTAGGATGGATTTTACC
AGCACTTATTGGTGGATTGATCGGGTACGTCATTAGCATTGTGAAAGCAAAAAATCAAGTTCAACCAGCAGCTAGTACGA
ATAAGAAAATAGGTTAA

Upstream 100 bases:

>100_bases
ATATTATATATCAAACTATAATGAGTTCAAGAATAGTTGATACTCAAGTTAAATATTCAGAAAATTCAATTAGTTAAAAA
TGGAAATGACATAGGTTATT

Downstream 100 bases:

>100_bases
ATAAAAAAGTTATAGAACTTGTTTCTATAACTTTTTTTGTTACTATTAATAATGAGAGAAAATAAAGTAGTAGGGGAAAT
AAAATGAAAAAAACAATTGA

Product: branched-chain amino acid transport system carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MNGGYIMTNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGVLAFGISGKEDLQSL
ASRVHPLFGIVFTTVLYLAIGPLFAIPRTGSVSYEIGMKPFVPEAMSSSALILFTIIFFSITCFFSLNPAKIVDIVGKIL
TPIKLTFIGILVVFAFIHPMGEMQAPAEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIINAIKEKGAKTKKDIMIVCAKA
TLIAASILAMIYSALSFMGASSVAKLGHLDNGGAVLAKVSNYYFGSYGGIILGIMVTAACLTTSVGLVSACSSFFHKLFP
NVSYKKIAIILSVFSAVVANVGLTQLIAISVPVLIAIYPLAIVLIFLTLLHPLFKGRAEVYQGSLLITFIVSVFDGLNAA
GMKLAVVNDFLTKILPMHEVGLGWILPALIGGLIGYVISIVKAKNQVQPAASTNKKIG

Sequences:

>Translated_458_residues
MNGGYIMTNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGVLAFGISGKEDLQSL
ASRVHPLFGIVFTTVLYLAIGPLFAIPRTGSVSYEIGMKPFVPEAMSSSALILFTIIFFSITCFFSLNPAKIVDIVGKIL
TPIKLTFIGILVVFAFIHPMGEMQAPAEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIINAIKEKGAKTKKDIMIVCAKA
TLIAASILAMIYSALSFMGASSVAKLGHLDNGGAVLAKVSNYYFGSYGGIILGIMVTAACLTTSVGLVSACSSFFHKLFP
NVSYKKIAIILSVFSAVVANVGLTQLIAISVPVLIAIYPLAIVLIFLTLLHPLFKGRAEVYQGSLLITFIVSVFDGLNAA
GMKLAVVNDFLTKILPMHEVGLGWILPALIGGLIGYVISIVKAKNQVQPAASTNKKIG
>Mature_458_residues
MNGGYIMTNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGVLAFGISGKEDLQSL
ASRVHPLFGIVFTTVLYLAIGPLFAIPRTGSVSYEIGMKPFVPEAMSSSALILFTIIFFSITCFFSLNPAKIVDIVGKIL
TPIKLTFIGILVVFAFIHPMGEMQAPAEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIINAIKEKGAKTKKDIMIVCAKA
TLIAASILAMIYSALSFMGASSVAKLGHLDNGGAVLAKVSNYYFGSYGGIILGIMVTAACLTTSVGLVSACSSFFHKLFP
NVSYKKIAIILSVFSAVVANVGLTQLIAISVPVLIAIYPLAIVLIFLTLLHPLFKGRAEVYQGSLLITFIVSVFDGLNAA
GMKLAVVNDFLTKILPMHEVGLGWILPALIGGLIGYVISIVKAKNQVQPAASTNKKIG

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=427, Percent_Identity=39.8126463700234, Blast_Score=300, Evalue=2e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 48582; Mature: 48582

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGGYIMTNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVG
CCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECCCHHHHHHH
LPLLGVLAFGISGKEDLQSLASRVHPLFGIVFTTVLYLAIGPLFAIPRTGSVSYEIGMKP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCC
FVPEAMSSSALILFTIIFFSITCFFSLNPAKIVDIVGKILTPIKLTFIGILVVFAFIHPM
CCCHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GEMQAPAEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIINAIKEKGAKTKKDIMIVCAKA
CCCCCCHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHH
TLIAASILAMIYSALSFMGASSVAKLGHLDNGGAVLAKVSNYYFGSYGGIILGIMVTAAC
HHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHHHHH
LTTSVGLVSACSSFFHKLFPNVSYKKIAIILSVFSAVVANVGLTQLIAISVPVLIAIYPL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AIVLIFLTLLHPLFKGRAEVYQGSLLITFIVSVFDGLNAAGMKLAVVNDFLTKILPMHEV
HHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
GLGWILPALIGGLIGYVISIVKAKNQVQPAASTNKKIG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNGGYIMTNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVG
CCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECCCHHHHHHH
LPLLGVLAFGISGKEDLQSLASRVHPLFGIVFTTVLYLAIGPLFAIPRTGSVSYEIGMKP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCC
FVPEAMSSSALILFTIIFFSITCFFSLNPAKIVDIVGKILTPIKLTFIGILVVFAFIHPM
CCCHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GEMQAPAEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIINAIKEKGAKTKKDIMIVCAKA
CCCCCCHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHH
TLIAASILAMIYSALSFMGASSVAKLGHLDNGGAVLAKVSNYYFGSYGGIILGIMVTAAC
HHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHHHHH
LTTSVGLVSACSSFFHKLFPNVSYKKIAIILSVFSAVVANVGLTQLIAISVPVLIAIYPL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AIVLIFLTLLHPLFKGRAEVYQGSLLITFIVSVFDGLNAAGMKLAVVNDFLTKILPMHEV
HHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
GLGWILPALIGGLIGYVISIVKAKNQVQPAASTNKKIG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]