Click here to switch to the map view.

The map label for this gene is yfiS [H]

Identifier: 118477158

GI number: 118477158

Start: 1612807

End: 1614075

Strand: Reverse

Name: yfiS [H]

Synonym: BALH_1463

Alternate gene names: 118477158

Gene position: 1614075-1612807 (Counterclockwise)

Preceding gene: 118477159

Following gene: 118477157

Centisome position: 30.7

GC content: 32.62

Gene sequence:

>1269_bases
GTGAATGGAAAAAACAAATTCAAGCATCATCATTACATAAGGATTGAGCTTTTTATGTCAGTTTCTTATTCCGCACTTCT
TAAAACAAATAAAAACTTCAACAAACTATTTTACGGACAAACATTAAGTGTACTTGGAGATTGGTTTCATACAGTAGCTT
TATTAACATTCGTCTATAGCATAACAGAATCTCCGTTTATGATTGCACTTACTTTTATAAGTAAAGGTTTACCACAACTA
CTTCTTAGCCCTTTCATAGGCGGAATGGTAGATCGTTTTTCTAAAAAGAATATTATGATTTTCACCGATATTACACGGGG
AATTCTAGTCCTTACATACATACTCGCAAGCTATAAAATTGAAATTATTTTCATTGCTAATATATGTTTATCTGTACTTT
CTTGCTTATTTGAGCCAGCTAAACAAGCAACCTTAAAAAACATCGTACACGAAAATCATTTCGTAACTGCTAACTCTCTT
TCTAGTACAATGAATGGTTTTATGTCTATTATGGGTGCTTCTTTAGGCGGTATAATTGCACAATCTTTACATATTGAGTT
CGCTTTTTTAGTAAATAGCCTATCATACTTTATTTCAGCCTATTTTATTTATAGTATGTGTATCCCTTCTCATAATACTT
GTAACAAGAAAAAAGCATTTTTGACTGATATAAAAGATGGTTATACATATATATCGCAAACAAAAATCATTTTAACATTA
ATTCTTGTCGGCATTTCATGGGGGCTTATTGGCGGCGCTTATCAGCTACTTCTAACAATCTATGCCGAAAAAATTTTCCA
CACGAACATTGGGATTTTATATACGGTTCAAGGGGCTGGGCTTATGATAGGAAGCTTTCTCGTTAATCTTTATATATCCC
ATAATAAAGAAAAAATGAAAAAAGCATTTGGCTGGGCCTACTTCCTACAAGGCATTTTCTTTCTTGGATTCATTCTATCC
AATCAACTTATTTTCGGTATCATTACATTACTATGTATGCGCATAGCAGGAGGAATCATCGTTCCACTTGATACGACATT
ACTTCAAACTTACACGCGGGAAAATATGATTGGTAAAGTTTTCTCCTTTCATTACTCTATTTATGGCTCACTTATACAGT
TATCTATGCTCATGACAGGATGGTTATTAGAAATCCTTTCTCCTCAAATGATTGGTAGTTTATTAGCTACATGCTGTATA
TTTGTAAGTTTTATATGGCTTTATCTGTTTTATCGCGGGAAACTTAGTGAAGAATCTACTATTACTTAA

Upstream 100 bases:

>100_bases
GCACATGCCGCTGTATACTTTAACGATCCTGATGGAAATAGTTTAGAATTGATTGCACAATTACCTGTTGGACTTCCTTC
AGAAAAGAAAGTGTATTTAA

Downstream 100 bases:

>100_bases
TAGCATGTTTATGTACATGCTATTTTTATTTATGTCGAAATACAGAGGAAAAAGGCGATTTATTTTCCTTACGTTCTAAT
TTTTTAGAATATACAATAAA

Product: macrolide efflux protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNGKNKFKHHHYIRIELFMSVSYSALLKTNKNFNKLFYGQTLSVLGDWFHTVALLTFVYSITESPFMIALTFISKGLPQL
LLSPFIGGMVDRFSKKNIMIFTDITRGILVLTYILASYKIEIIFIANICLSVLSCLFEPAKQATLKNIVHENHFVTANSL
SSTMNGFMSIMGASLGGIIAQSLHIEFAFLVNSLSYFISAYFIYSMCIPSHNTCNKKKAFLTDIKDGYTYISQTKIILTL
ILVGISWGLIGGAYQLLLTIYAEKIFHTNIGILYTVQGAGLMIGSFLVNLYISHNKEKMKKAFGWAYFLQGIFFLGFILS
NQLIFGIITLLCMRIAGGIIVPLDTTLLQTYTRENMIGKVFSFHYSIYGSLIQLSMLMTGWLLEILSPQMIGSLLATCCI
FVSFIWLYLFYRGKLSEESTIT

Sequences:

>Translated_422_residues
MNGKNKFKHHHYIRIELFMSVSYSALLKTNKNFNKLFYGQTLSVLGDWFHTVALLTFVYSITESPFMIALTFISKGLPQL
LLSPFIGGMVDRFSKKNIMIFTDITRGILVLTYILASYKIEIIFIANICLSVLSCLFEPAKQATLKNIVHENHFVTANSL
SSTMNGFMSIMGASLGGIIAQSLHIEFAFLVNSLSYFISAYFIYSMCIPSHNTCNKKKAFLTDIKDGYTYISQTKIILTL
ILVGISWGLIGGAYQLLLTIYAEKIFHTNIGILYTVQGAGLMIGSFLVNLYISHNKEKMKKAFGWAYFLQGIFFLGFILS
NQLIFGIITLLCMRIAGGIIVPLDTTLLQTYTRENMIGKVFSFHYSIYGSLIQLSMLMTGWLLEILSPQMIGSLLATCCI
FVSFIWLYLFYRGKLSEESTIT
>Mature_422_residues
MNGKNKFKHHHYIRIELFMSVSYSALLKTNKNFNKLFYGQTLSVLGDWFHTVALLTFVYSITESPFMIALTFISKGLPQL
LLSPFIGGMVDRFSKKNIMIFTDITRGILVLTYILASYKIEIIFIANICLSVLSCLFEPAKQATLKNIVHENHFVTANSL
SSTMNGFMSIMGASLGGIIAQSLHIEFAFLVNSLSYFISAYFIYSMCIPSHNTCNKKKAFLTDIKDGYTYISQTKIILTL
ILVGISWGLIGGAYQLLLTIYAEKIFHTNIGILYTVQGAGLMIGSFLVNLYISHNKEKMKKAFGWAYFLQGIFFLGFILS
NQLIFGIITLLCMRIAGGIIVPLDTTLLQTYTRENMIGKVFSFHYSIYGSLIQLSMLMTGWLLEILSPQMIGSLLATCCI
FVSFIWLYLFYRGKLSEESTIT

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47579; Mature: 47579

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGKNKFKHHHYIRIELFMSVSYSALLKTNKNFNKLFYGQTLSVLGDWFHTVALLTFVYS
CCCCCCCCCCEEEEEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITESPFMIALTFISKGLPQLLLSPFIGGMVDRFSKKNIMIFTDITRGILVLTYILASYKI
HCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHE
EIIFIANICLSVLSCLFEPAKQATLKNIVHENHFVTANSLSSTMNGFMSIMGASLGGIIA
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
QSLHIEFAFLVNSLSYFISAYFIYSMCIPSHNTCNKKKAFLTDIKDGYTYISQTKIILTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
ILVGISWGLIGGAYQLLLTIYAEKIFHTNIGILYTVQGAGLMIGSFLVNLYISHNKEKMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHH
KAFGWAYFLQGIFFLGFILSNQLIFGIITLLCMRIAGGIIVPLDTTLLQTYTRENMIGKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHH
FSFHYSIYGSLIQLSMLMTGWLLEILSPQMIGSLLATCCIFVSFIWLYLFYRGKLSEEST
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IT
CC
>Mature Secondary Structure
MNGKNKFKHHHYIRIELFMSVSYSALLKTNKNFNKLFYGQTLSVLGDWFHTVALLTFVYS
CCCCCCCCCCEEEEEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITESPFMIALTFISKGLPQLLLSPFIGGMVDRFSKKNIMIFTDITRGILVLTYILASYKI
HCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHE
EIIFIANICLSVLSCLFEPAKQATLKNIVHENHFVTANSLSSTMNGFMSIMGASLGGIIA
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
QSLHIEFAFLVNSLSYFISAYFIYSMCIPSHNTCNKKKAFLTDIKDGYTYISQTKIILTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
ILVGISWGLIGGAYQLLLTIYAEKIFHTNIGILYTVQGAGLMIGSFLVNLYISHNKEKMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHH
KAFGWAYFLQGIFFLGFILSNQLIFGIITLLCMRIAGGIIVPLDTTLLQTYTRENMIGKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHH
FSFHYSIYGSLIQLSMLMTGWLLEILSPQMIGSLLATCCIFVSFIWLYLFYRGKLSEEST
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IT
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]