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The map label for this gene is glcF [H]

Identifier: 118476869

GI number: 118476869

Start: 1314742

End: 1316070

Strand: Reverse

Name: glcF [H]

Synonym: BALH_1158

Alternate gene names: 118476869

Gene position: 1316070-1314742 (Counterclockwise)

Preceding gene: 118476870

Following gene: 118476868

Centisome position: 25.03

GC content: 37.47

Gene sequence:

>1329_bases
ATGACAACATTAAATAAAGAAAACATTCAAAAAGAATTTAAAGAACGATTAAGTGAAGATGAACTACTAAACTGCATGCG
ATGCGGTTTCTGTTTACCAACTTGCCCTACTTACATTCAATCTGGATATAAAGAATCACATTCACCACGTGGGCGTATTG
CATTAATGAAAGCGGTCGTTGACGGTTTAATTGAGCCAGATGAAGATGTTGAAAACACATTAAACGTTTGCCTTGGCTGC
CGTGCTTGTGAACCCGTTTGTCCATCTGGTGTGAATTATGGCCATTTATTAGAAGAAGCCCGTGATATTATAAATCAAAA
CAAAAAGTTCTCGATGCCAGTGAGAGCCGTTCGTAAAGTCGTTTTTGAAGGACTATTCCCGCATCAAAATCGAATGAGAA
CATTAACTGGACTAATTGGGTTTTATCAGCGTTCTGGTCTACAAACGTTAACACATAAAACAGGGATTATGAAGTTATTC
CCTGAAACACTTGCAACAATGGATCTCGTTTTGCCTAAGGTTCCTAAAATGAAAGCAATGAAAGATCGCCCTGCACTTTT
ACATGCTGAAAGTACAAAGAAGAAACAAGTTGCATTCTTCACAGGTTGTTTAATGGATACAATGTTTTTAGAAACAAATA
ACGCAACGATGAAACTTCTCCAGTTAGCTGGTTGTGATATCGTTATTCCGAAAACACAAAGTTGCTGCGGGGCATTACAT
GGGCATGCTGGTGAGAAAAGTGGAGCAAAAGAATTAGCGAAACGCAATATAAAAGCATTCGAAGATTTAAACATTGATTA
TATTATTACGAATGCTGGTGGTTGCGGAGCTTACCTCGTAGACTACGATTATCTTTTAAAAGATGATCCACAGTGGGCTG
AACGTGCAAAACAGTTTGTTTCCAAAATTAAAGATATTACTGCTATTCTAGTAGAACTAGATTTCCATAAACGTAATGAC
TTACGACTCACGCCGCAAATTATTACGTATCAAGACTCTTGTCATTTACGCAATGTTATGCGAACATCTTCAGAACCCCG
TATGTTACTAGAAGCAATTCAAGGCGCAACATACCGCGAAATGAAAGATGCGGATCGCTGTTGTGGTTCTGCTGGTATTT
ACAATATTGTTCATTCAGAGTTATCAATGGAATTTCTAGATTACAAAATGGACCGTGTGCATGAAACAGACGCAACAACC
ATTGTTACTGCGAATCCAGGTTGTTTATTACAAATGAAATTAGGTATTGAACGAGAAGGTCTTTCTCATAAAATGAGAGG
AATTCACATTGTTGATTTATTATTAGAAGCAATTGAAACCAACTCGTAA

Upstream 100 bases:

>100_bases
CTGCTATGCAAGGGATTAAACAAGCCTTCGATCCAAATAATATTATGAATCCAGGAAAGATGTTCGCGAAGGACACTCGT
AAAAGAGTGGTGGTTGAGCG

Downstream 100 bases:

>100_bases
TAACTCGTAACATACGTAATAATAGGAAAACGGCTATCTCCTTCGTCTATGTAAAGAGATAGCCGCTTTTTTGTCCGCTA
CAACATTAAAAATATGTTAC

Product: (S)-2-hydroxy-acid oxidase

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTTLNKENIQKEFKERLSEDELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVVDGLIEPDEDVENTLNVCLGC
RACEPVCPSGVNYGHLLEEARDIINQNKKFSMPVRAVRKVVFEGLFPHQNRMRTLTGLIGFYQRSGLQTLTHKTGIMKLF
PETLATMDLVLPKVPKMKAMKDRPALLHAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALH
GHAGEKSGAKELAKRNIKAFEDLNIDYIITNAGGCGAYLVDYDYLLKDDPQWAERAKQFVSKIKDITAILVELDFHKRND
LRLTPQIITYQDSCHLRNVMRTSSEPRMLLEAIQGATYREMKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETDATT
IVTANPGCLLQMKLGIEREGLSHKMRGIHIVDLLLEAIETNS

Sequences:

>Translated_442_residues
MTTLNKENIQKEFKERLSEDELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVVDGLIEPDEDVENTLNVCLGC
RACEPVCPSGVNYGHLLEEARDIINQNKKFSMPVRAVRKVVFEGLFPHQNRMRTLTGLIGFYQRSGLQTLTHKTGIMKLF
PETLATMDLVLPKVPKMKAMKDRPALLHAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALH
GHAGEKSGAKELAKRNIKAFEDLNIDYIITNAGGCGAYLVDYDYLLKDDPQWAERAKQFVSKIKDITAILVELDFHKRND
LRLTPQIITYQDSCHLRNVMRTSSEPRMLLEAIQGATYREMKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETDATT
IVTANPGCLLQMKLGIEREGLSHKMRGIHIVDLLLEAIETNS
>Mature_441_residues
TTLNKENIQKEFKERLSEDELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVVDGLIEPDEDVENTLNVCLGCR
ACEPVCPSGVNYGHLLEEARDIINQNKKFSMPVRAVRKVVFEGLFPHQNRMRTLTGLIGFYQRSGLQTLTHKTGIMKLFP
ETLATMDLVLPKVPKMKAMKDRPALLHAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALHG
HAGEKSGAKELAKRNIKAFEDLNIDYIITNAGGCGAYLVDYDYLLKDDPQWAERAKQFVSKIKDITAILVELDFHKRNDL
RLTPQIITYQDSCHLRNVMRTSSEPRMLLEAIQGATYREMKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETDATTI
VTANPGCLLQMKLGIEREGLSHKMRGIHIVDLLLEAIETNS

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=444, Percent_Identity=31.3063063063063, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1786497, Length=244, Percent_Identity=29.0983606557377, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: 1.1.3.15

Molecular weight: Translated: 49749; Mature: 49618

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLNKENIQKEFKERLSEDELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVV
CCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHH
DGLIEPDEDVENTLNVCLGCRACEPVCPSGVNYGHLLEEARDIINQNKKFSMPVRAVRKV
HHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VFEGLFPHQNRMRTLTGLIGFYQRSGLQTLTHKTGIMKLFPETLATMDLVLPKVPKMKAM
HHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
KDRPALLHAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALH
CCCCCEEECCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCCEEECCCHHHHHHHH
GHAGEKSGAKELAKRNIKAFEDLNIDYIITNAGGCGAYLVDYDYLLKDDPQWAERAKQFV
CCCCCCCHHHHHHHHCHHHHHCCCCEEEEECCCCCCEEEEECHHHHCCCHHHHHHHHHHH
SKIKDITAILVELDFHKRNDLRLTPQIITYQDSCHLRNVMRTSSEPRMLLEAIQGATYRE
HHHHHHHHHHHEECCCCCCCCEECCEEEEECCCHHHHHHHHCCCCHHHHHHHHCCCHHHH
MKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETDATTIVTANPGCLLQMKLGIEREG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCC
LSHKMRGIHIVDLLLEAIETNS
CHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTLNKENIQKEFKERLSEDELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVV
CCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHH
DGLIEPDEDVENTLNVCLGCRACEPVCPSGVNYGHLLEEARDIINQNKKFSMPVRAVRKV
HHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VFEGLFPHQNRMRTLTGLIGFYQRSGLQTLTHKTGIMKLFPETLATMDLVLPKVPKMKAM
HHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
KDRPALLHAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALH
CCCCCEEECCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCCEEECCCHHHHHHHH
GHAGEKSGAKELAKRNIKAFEDLNIDYIITNAGGCGAYLVDYDYLLKDDPQWAERAKQFV
CCCCCCCHHHHHHHHCHHHHHCCCCEEEEECCCCCCEEEEECHHHHCCCHHHHHHHHHHH
SKIKDITAILVELDFHKRNDLRLTPQIITYQDSCHLRNVMRTSSEPRMLLEAIQGATYRE
HHHHHHHHHHHEECCCCCCCCEECCEEEEECCCHHHHHHHHCCCCHHHHHHHHCCCHHHH
MKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETDATTIVTANPGCLLQMKLGIEREG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCC
LSHKMRGIHIVDLLLEAIETNS
CHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]