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The map label for this gene is azoR1 [H]

Identifier: 118476598

GI number: 118476598

Start: 1002540

End: 1003181

Strand: Reverse

Name: azoR1 [H]

Synonym: BALH_0873

Alternate gene names: 118476598

Gene position: 1003181-1002540 (Counterclockwise)

Preceding gene: 118476607

Following gene: 118476596

Centisome position: 19.08

GC content: 31.46

Gene sequence:

>642_bases
ATGAACAAAACACTTATCATAAACGCACATCCGAAAGTGGATGATACATCATCAGTAAGCATTAAGGTTTTTAAGCATTT
TTTAGAATCTTATAAAGAATTAATTTCTAATAATAAAACGATTGAACAGATTAATTTATACGATGATGTAGTACCAATGA
TAGATAAAACTGTTTTAAGCGCATGGGAAAAACAAGGTAATGGACAAGAGTTGACTCGTGAAGAACAAAAAGTAACGGAA
CGTATGTCCGAGATTTTACAACAATTTAAAAGTGCAAATACGTATGTTATCGTATTCCCTTTGCACAACTTTAATATTCC
ATCAAAGTTAAAAGATTACATGGACAATATCATGATTGCACGTGAAACATTTAAATATACTGAAACTGGATCAGTAGGAT
TACTTAAAGATGGAAGAAGAATGCTTGTTATACAAGCAAGTGGAGGAATCTATACAAACGATAATTGGTATACAGAAGTG
GAATACTCTCATAAATATTTAAAAGCAATGTTTAATTTCCTTGGTATTGAAGATTATCAAATCGTTCGTGCACAAGGAAC
AGCAGTACTAGATCCAACTGAAGTATTACAAAACGCATATAAAGAAGTTGAAGAGGCTGCTTCTAGATTGGCTAACAAAT
AA

Upstream 100 bases:

>100_bases
GACAAAAATAATGTCAGAGTTTATATTAAAGACCAAGTTAATCGCCGATTAACTTGGTCGACAATTAAAGCGTAAAAACT
TTTAAAAAGGGGAAATACAA

Downstream 100 bases:

>100_bases
ATTTTTTCACTGGAAGAATGATGGTTATAAATAAAGATCCCCTCCACCTTAAGGCAGAGGGGACTTTCCAATTCCTATTT
CAAACTACCCGCAATCTCCG

Product: acyl carrier protein phosphodiesterase

Products: 4'- Phosphopantetheine; Apoprotein. [C]

Alternate protein names: Azo-dye reductase 1; FMN-dependent NADH-azo compound oxidoreductase 1 [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MNKTLIINAHPKVDDTSSVSIKVFKHFLESYKELISNNKTIEQINLYDDVVPMIDKTVLSAWEKQGNGQELTREEQKVTE
RMSEILQQFKSANTYVIVFPLHNFNIPSKLKDYMDNIMIARETFKYTETGSVGLLKDGRRMLVIQASGGIYTNDNWYTEV
EYSHKYLKAMFNFLGIEDYQIVRAQGTAVLDPTEVLQNAYKEVEEAASRLANK

Sequences:

>Translated_213_residues
MNKTLIINAHPKVDDTSSVSIKVFKHFLESYKELISNNKTIEQINLYDDVVPMIDKTVLSAWEKQGNGQELTREEQKVTE
RMSEILQQFKSANTYVIVFPLHNFNIPSKLKDYMDNIMIARETFKYTETGSVGLLKDGRRMLVIQASGGIYTNDNWYTEV
EYSHKYLKAMFNFLGIEDYQIVRAQGTAVLDPTEVLQNAYKEVEEAASRLANK
>Mature_213_residues
MNKTLIINAHPKVDDTSSVSIKVFKHFLESYKELISNNKTIEQINLYDDVVPMIDKTVLSAWEKQGNGQELTREEQKVTE
RMSEILQQFKSANTYVIVFPLHNFNIPSKLKDYMDNIMIARETFKYTETGSVGLLKDGRRMLVIQASGGIYTNDNWYTEV
EYSHKYLKAMFNFLGIEDYQIVRAQGTAVLDPTEVLQNAYKEVEEAASRLANK

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=163, Percent_Identity=30.6748466257669, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14 [C]

Molecular weight: Translated: 24533; Mature: 24533

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTLIINAHPKVDDTSSVSIKVFKHFLESYKELISNNKTIEQINLYDDVVPMIDKTVLS
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
AWEKQGNGQELTREEQKVTERMSEILQQFKSANTYVIVFPLHNFNIPSKLKDYMDNIMIA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
RETFKYTETGSVGLLKDGRRMLVIQASGGIYTNDNWYTEVEYSHKYLKAMFNFLGIEDYQ
HHHHCCCCCCCEEEEECCCEEEEEEECCCEEECCCEEEEEHHHHHHHHHHHHHHCCCCCE
IVRAQGTAVLDPTEVLQNAYKEVEEAASRLANK
EEEECCCEEECHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKTLIINAHPKVDDTSSVSIKVFKHFLESYKELISNNKTIEQINLYDDVVPMIDKTVLS
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
AWEKQGNGQELTREEQKVTERMSEILQQFKSANTYVIVFPLHNFNIPSKLKDYMDNIMIA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
RETFKYTETGSVGLLKDGRRMLVIQASGGIYTNDNWYTEVEYSHKYLKAMFNFLGIEDYQ
HHHHCCCCCCCEEEEECCCEEEEEEECCCEEECCCEEEEEHHHHHHHHHHHHHHCCCCCE
IVRAQGTAVLDPTEVLQNAYKEVEEAASRLANK
EEEECCCEEECHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [Acyl-Carrier Protein]; H2O [C]

Specific reaction: [Acyl-Carrier Protein] + H2O = 4'- Phosphopantetheine + Apoprotein. [C]

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]