Click here to switch to the map view.

The map label for this gene is thiG

Identifier: 118476407

GI number: 118476407

Start: 778108

End: 778884

Strand: Direct

Name: thiG

Synonym: BALH_0669

Alternate gene names: 118476407

Gene position: 778108-778884 (Clockwise)

Preceding gene: 118476406

Following gene: 118476408

Centisome position: 14.8

GC content: 38.48

Gene sequence:

>777_bases
TTGATTATGTTAAACATTGGACCATTTTCATTTCATTCCAGACTTTTATTAGGAACAGGAAAATTCCCTGATTTTGATGT
ACAGCAAAAGGCAATTGATGTATCTGAGGCTGAGGTTTTAACCTTCGCAGTACGTCGTATGGATATATTTGATGCAAAGC
AGCCTAATTTATTAGAAAAACTTGATGTGAAAAAATATAAGTTATTACCGAATACAGCTGGAGCAAAAAATGCTGAAGAA
GCTGTTCGTATTGCAAAATTAGCAAAAGCTTCAGGGCTTTGTGACATGATTAAAGTTGAAGTTATTGGTGATGATAGAAC
GTTATTACCTGATCCGGTAGAAACGTTAAAGGCATCTGAAATGTTACTAGAAGAAGGATTTATCGTACTTCCGTACACAT
CTGATGATGTTGTATTAGCACGTAAATTACAAGAACTTGGTGTGCATGCGATTATGCCAGGAGCATCACCAATCGGTTCA
GGTCTTGGTATTGTAAATCCATTAAATCTAAGTTTTATTATTGAACAAGCGACAGTGCCAGTTATCGTTGACGCTGGTAT
CGGTAGCCCAGCTGATGCGGCATTCGCAATGGAATTAGGAGCGGATGGTGTGTTATTAAATACGGCTGTGTCAGGAGCAA
AAGATCCTATTAAGATGGCACAAGCAATGAAATTAAGTATTGAGGCAGGACGTTTAGGCTTTGAAGCAGGTCGTATTGCA
CGTAAACGTTGTGCAACGGCAAGTAGTCCTTTAGAAGGAATGAGCGTAGTTGAATAA

Upstream 100 bases:

>100_bases
TAGTAGAGCGTAATAAAGATATTTTACAAAAAGATGATCATACAGATACATCTGTTTTTGATGGAGACCAAATTGAGATT
GTAACTTTCGTAGGAGGCGG

Downstream 100 bases:

>100_bases
TCGATATTCTCGCCAAGAATTATTTTCTCCGATTGGGGAAGAAGGCCAGCAAAAGATAAGAGAAAAGCATGTGCTTATTA
TCGGCGCGGGCGCACTAGGT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MIMLNIGPFSFHSRLLLGTGKFPDFDVQQKAIDVSEAEVLTFAVRRMDIFDAKQPNLLEKLDVKKYKLLPNTAGAKNAEE
AVRIAKLAKASGLCDMIKVEVIGDDRTLLPDPVETLKASEMLLEEGFIVLPYTSDDVVLARKLQELGVHAIMPGASPIGS
GLGIVNPLNLSFIIEQATVPVIVDAGIGSPADAAFAMELGADGVLLNTAVSGAKDPIKMAQAMKLSIEAGRLGFEAGRIA
RKRCATASSPLEGMSVVE

Sequences:

>Translated_258_residues
MIMLNIGPFSFHSRLLLGTGKFPDFDVQQKAIDVSEAEVLTFAVRRMDIFDAKQPNLLEKLDVKKYKLLPNTAGAKNAEE
AVRIAKLAKASGLCDMIKVEVIGDDRTLLPDPVETLKASEMLLEEGFIVLPYTSDDVVLARKLQELGVHAIMPGASPIGS
GLGIVNPLNLSFIIEQATVPVIVDAGIGSPADAAFAMELGADGVLLNTAVSGAKDPIKMAQAMKLSIEAGRLGFEAGRIA
RKRCATASSPLEGMSVVE
>Mature_258_residues
MIMLNIGPFSFHSRLLLGTGKFPDFDVQQKAIDVSEAEVLTFAVRRMDIFDAKQPNLLEKLDVKKYKLLPNTAGAKNAEE
AVRIAKLAKASGLCDMIKVEVIGDDRTLLPDPVETLKASEMLLEEGFIVLPYTSDDVVLARKLQELGVHAIMPGASPIGS
GLGIVNPLNLSFIIEQATVPVIVDAGIGSPADAAFAMELGADGVLLNTAVSGAKDPIKMAQAMKLSIEAGRLGFEAGRIA
RKRCATASSPLEGMSVVE

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=254, Percent_Identity=47.6377952755905, Blast_Score=224, Evalue=5e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_BACAH (A0RA08)

Other databases:

- EMBL:   CP000485
- RefSeq:   YP_893558.1
- ProteinModelPortal:   A0RA08
- SMR:   A0RA08
- STRING:   A0RA08
- EnsemblBacteria:   EBBACT00000066604
- GeneID:   4546390
- GenomeReviews:   CP000485_GR
- KEGG:   btl:BALH_0669
- eggNOG:   COG2022
- GeneTree:   EBGT00050000002572
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- BioCyc:   BTHU412694:BALH_0669-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27444; Mature: 27444

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: ACT_SITE 98-98 BINDING 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMLNIGPFSFHSRLLLGTGKFPDFDVQQKAIDVSEAEVLTFAVRRMDIFDAKQPNLLEK
CEEEEECCCCCCCEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
LDVKKYKLLPNTAGAKNAEEAVRIAKLAKASGLCDMIKVEVIGDDRTLLPDPVETLKASE
HCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHH
MLLEEGFIVLPYTSDDVVLARKLQELGVHAIMPGASPIGSGLGIVNPLNLSFIIEQATVP
HHHHCCCEEEEECCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCC
VIVDAGIGSPADAAFAMELGADGVLLNTAVSGAKDPIKMAQAMKLSIEAGRLGFEAGRIA
EEEECCCCCCCCCEEEEECCCCCEEEEEHHCCCCCHHHHHHHHHEEEECCCCCCHHHHHH
RKRCATASSPLEGMSVVE
HHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MIMLNIGPFSFHSRLLLGTGKFPDFDVQQKAIDVSEAEVLTFAVRRMDIFDAKQPNLLEK
CEEEEECCCCCCCEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
LDVKKYKLLPNTAGAKNAEEAVRIAKLAKASGLCDMIKVEVIGDDRTLLPDPVETLKASE
HCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHH
MLLEEGFIVLPYTSDDVVLARKLQELGVHAIMPGASPIGSGLGIVNPLNLSFIIEQATVP
HHHHCCCEEEEECCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCC
VIVDAGIGSPADAAFAMELGADGVLLNTAVSGAKDPIKMAQAMKLSIEAGRLGFEAGRIA
EEEECCCCCCCCCEEEEECCCCCEEEEEHHCCCCCHHHHHHHHHEEEECCCCCCHHHHHH
RKRCATASSPLEGMSVVE
HHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA