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The map label for this gene is nprE [H]

Identifier: 118476285

GI number: 118476285

Start: 625608

End: 627308

Strand: Direct

Name: nprE [H]

Synonym: BALH_0539

Alternate gene names: 118476285

Gene position: 625608-627308 (Clockwise)

Preceding gene: 118476284

Following gene: 118476287

Centisome position: 11.9

GC content: 37.92

Gene sequence:

>1701_bases
ATGAAAAAGAAAAGTTTAGCGTTAGTGTTAGCGACAGGAATGGCAGTTACAACGTTTGGAGGGACAGGCTCTGCTTTTGC
AGATTCTAAAAATGTGCTCTCTACGAAGAAGTACAATGAGACAGTACAGTCACCGGAGTTTATTTCTGGGGATTTAACTG
AAGCAACTGGTAAGAAAGCAGAATCTGTTGTGTTTGATTACTTAAATGCAGCAAAAGGTGATTATAAGTTAGGGGAAAAG
AGTGCGCAAGATTCTTTCAAAGTGAAACAAGCGAAGAAAGATGCTGTAACTGATTCAACAGTATTACGTTTGCAACAAGT
TTACGAAGGAGTACCTGTATGGGGTTCTACGCAAGTAGCTCACGTAAGTAAAGATGGTTCTTTAAAAGTATTGTCTGGAA
CAGTTGCACCTGATTTAGACAAAAAAGAAAAGTTGAAAAATAAAAATAAGATCGAAGGCGCAAAAGCAATTGAAATTGCG
CAAAAAGATTTAGGGGTAACACCTAAATATGAGGTAGAACCAAAAGCGGACTTATATGTATATCAAAACGGTGAAGAAAC
AACATATGCATACGTTGTAAATTTAAACTTCTTAGAGCCAAGCCCAGGAAACTACTACTATTTCATTGAAGCAGACAGCG
GTAAAGTATTAAATAAATATAATAAATTGGATCATGTAGCAAATGAAGATAAGTCACCAGTTAAGCAAGAGGCACCTAAA
CAAGAAGCGAAACCAGCTGTAAAGCCTGTAACAGGCACAAATGCAGTGGGTACTGGTAAAGGTGTATTAGGAGATACGAA
GTCACTTAATACAACGTTATCTGCATCATCTTACTATTTACAAGATAATACGCGCGGAGCAACGATTTTCACATATGATG
CGAAAAATCGCTCAACATTACCAGGAACGTTATGGGTAGATGCGGATAATGTTTTCAATGCAGCGTATGATGCAGCGGCA
GTAGACGCTCATTACTATGCGGGTAAAACATATGATTACTATAAAGCTACATTTAATAGAAACTCTATTAATGATGCAGG
AGCGCCATTAAAATCAACAGTTCATTATGGAAGTAGATATAATAATGCGTTCTGGAATGGCTCTCAAATGGTATACGGAG
ATGGTGATGGTGTAACATTCACTTCATTGTCTGGTGGAATTGATGTAATTGGCCATGAATTAACGCATGCTGTTACAGAG
TATAGCTCAGATTTAATCTATCAAAATGAATCAGGAGCATTAAATGAAGCTATTTCAGATGTATTTGGTACTTTAGTAGA
ATTCTATGATAACCGTAACCCAGATTGGGAAATTGGTGAAGATATTTATACGCCTGGTAAAGCAGGAGATGCGCTTCGCT
CTATGAGTGATCCAACAAAATATGGTGATCCAGATCATTATTCTAAGCGTTACACAGGTACTGGTGATAACGGTGGCGTT
CATACAAATAGCGGTATTATTAACAAAGCGGCTTACTTATTAGCGAATGGTGGTACGCATTACGGTGTTACTGTGAACGG
TATTGGTAAAGATAAAGTAGGAGCGATTTATTACCGTGCAAATACGCAATATTTCACGCAATCTACTACTTTTAGTCAAG
CTCGTGCTGGATTAGTACAAGCTGCAACTGACTTATATGGTGCTAGCTCTGCAGAAGTAGCAGCAGTTAAGCAATCATAT
AGTGCTGTTGGTGTAAACTAA

Upstream 100 bases:

>100_bases
TGAGAAATAAATTAATTGAATTTTTAGTATATTAATAGTGAAAACATAATGCTAATATGAAACTACTCTTTTTCAAAAAA
AATTATTAGGGGGAAGGTTC

Downstream 100 bases:

>100_bases
GGATTTTAGAGATTATTAATAAAATACCTCAAAAATAAAGAAGGAGCATAGGCTCCTTCTTTATTTTTTTCTCCATTTTT
TCCACAAATAAAACAATCCA

Product: thermolysin

Products: NA

Alternate protein names: Neutral protease [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKKKSLALVLATGMAVTTFGGTGSAFADSKNVLSTKKYNETVQSPEFISGDLTEATGKKAESVVFDYLNAAKGDYKLGEK
SAQDSFKVKQAKKDAVTDSTVLRLQQVYEGVPVWGSTQVAHVSKDGSLKVLSGTVAPDLDKKEKLKNKNKIEGAKAIEIA
QKDLGVTPKYEVEPKADLYVYQNGEETTYAYVVNLNFLEPSPGNYYYFIEADSGKVLNKYNKLDHVANEDKSPVKQEAPK
QEAKPAVKPVTGTNAVGTGKGVLGDTKSLNTTLSASSYYLQDNTRGATIFTYDAKNRSTLPGTLWVDADNVFNAAYDAAA
VDAHYYAGKTYDYYKATFNRNSINDAGAPLKSTVHYGSRYNNAFWNGSQMVYGDGDGVTFTSLSGGIDVIGHELTHAVTE
YSSDLIYQNESGALNEAISDVFGTLVEFYDNRNPDWEIGEDIYTPGKAGDALRSMSDPTKYGDPDHYSKRYTGTGDNGGV
HTNSGIINKAAYLLANGGTHYGVTVNGIGKDKVGAIYYRANTQYFTQSTTFSQARAGLVQAATDLYGASSAEVAAVKQSY
SAVGVN

Sequences:

>Translated_566_residues
MKKKSLALVLATGMAVTTFGGTGSAFADSKNVLSTKKYNETVQSPEFISGDLTEATGKKAESVVFDYLNAAKGDYKLGEK
SAQDSFKVKQAKKDAVTDSTVLRLQQVYEGVPVWGSTQVAHVSKDGSLKVLSGTVAPDLDKKEKLKNKNKIEGAKAIEIA
QKDLGVTPKYEVEPKADLYVYQNGEETTYAYVVNLNFLEPSPGNYYYFIEADSGKVLNKYNKLDHVANEDKSPVKQEAPK
QEAKPAVKPVTGTNAVGTGKGVLGDTKSLNTTLSASSYYLQDNTRGATIFTYDAKNRSTLPGTLWVDADNVFNAAYDAAA
VDAHYYAGKTYDYYKATFNRNSINDAGAPLKSTVHYGSRYNNAFWNGSQMVYGDGDGVTFTSLSGGIDVIGHELTHAVTE
YSSDLIYQNESGALNEAISDVFGTLVEFYDNRNPDWEIGEDIYTPGKAGDALRSMSDPTKYGDPDHYSKRYTGTGDNGGV
HTNSGIINKAAYLLANGGTHYGVTVNGIGKDKVGAIYYRANTQYFTQSTTFSQARAGLVQAATDLYGASSAEVAAVKQSY
SAVGVN
>Mature_566_residues
MKKKSLALVLATGMAVTTFGGTGSAFADSKNVLSTKKYNETVQSPEFISGDLTEATGKKAESVVFDYLNAAKGDYKLGEK
SAQDSFKVKQAKKDAVTDSTVLRLQQVYEGVPVWGSTQVAHVSKDGSLKVLSGTVAPDLDKKEKLKNKNKIEGAKAIEIA
QKDLGVTPKYEVEPKADLYVYQNGEETTYAYVVNLNFLEPSPGNYYYFIEADSGKVLNKYNKLDHVANEDKSPVKQEAPK
QEAKPAVKPVTGTNAVGTGKGVLGDTKSLNTTLSASSYYLQDNTRGATIFTYDAKNRSTLPGTLWVDADNVFNAAYDAAA
VDAHYYAGKTYDYYKATFNRNSINDAGAPLKSTVHYGSRYNNAFWNGSQMVYGDGDGVTFTSLSGGIDVIGHELTHAVTE
YSSDLIYQNESGALNEAISDVFGTLVEFYDNRNPDWEIGEDIYTPGKAGDALRSMSDPTKYGDPDHYSKRYTGTGDNGGV
HTNSGIINKAAYLLANGGTHYGVTVNGIGKDKVGAIYYRANTQYFTQSTTFSQARAGLVQAATDLYGASSAEVAAVKQSY
SAVGVN

Specific function: Extracellular zinc metalloprotease [H]

COG id: COG3227

COG function: function code E; Zinc metalloprotease (elastase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005075
- InterPro:   IPR013856
- InterPro:   IPR001570
- InterPro:   IPR011096 [H]

Pfam domain/function: PF07504 FTP; PF03413 PepSY; PF01447 Peptidase_M4; PF02868 Peptidase_M4_C [H]

EC number: =3.4.24.28 [H]

Molecular weight: Translated: 60991; Mature: 60991

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKSLALVLATGMAVTTFGGTGSAFADSKNVLSTKKYNETVQSPEFISGDLTEATGKKA
CCCCEEEEEEECCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHCCCHH
ESVVFDYLNAAKGDYKLGEKSAQDSFKVKQAKKDAVTDSTVLRLQQVYEGVPVWGSTQVA
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEE
HVSKDGSLKVLSGTVAPDLDKKEKLKNKNKIEGAKAIEIAQKDLGVTPKYEVEPKADLYV
EECCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEE
YQNGEETTYAYVVNLNFLEPSPGNYYYFIEADSGKVLNKYNKLDHVANEDKSPVKQEAPK
EECCCCEEEEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHCCH
QEAKPAVKPVTGTNAVGTGKGVLGDTKSLNTTLSASSYYLQDNTRGATIFTYDAKNRSTL
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEECCCCCCEEEEEECCCCCCC
PGTLWVDADNVFNAAYDAAAVDAHYYAGKTYDYYKATFNRNSINDAGAPLKSTVHYGSRY
CEEEEEECCHHHHHHHHHHHHCCEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCCC
NNAFWNGSQMVYGDGDGVTFTSLSGGIDVIGHELTHAVTEYSSDLIYQNESGALNEAISD
CCCEECCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHH
VFGTLVEFYDNRNPDWEIGEDIYTPGKAGDALRSMSDPTKYGDPDHYSKRYTGTGDNGGV
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCE
HTNSGIINKAAYLLANGGTHYGVTVNGIGKDKVGAIYYRANTQYFTQSTTFSQARAGLVQ
ECCCCCHHCEEEEEECCCEEEEEEEECCCCCCCEEEEEEECCEEEECCCCHHHHHHHHHH
AATDLYGASSAEVAAVKQSYSAVGVN
HHHHHCCCCCCHHHHHHHCCHHCCCC
>Mature Secondary Structure
MKKKSLALVLATGMAVTTFGGTGSAFADSKNVLSTKKYNETVQSPEFISGDLTEATGKKA
CCCCEEEEEEECCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHCCCHH
ESVVFDYLNAAKGDYKLGEKSAQDSFKVKQAKKDAVTDSTVLRLQQVYEGVPVWGSTQVA
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEE
HVSKDGSLKVLSGTVAPDLDKKEKLKNKNKIEGAKAIEIAQKDLGVTPKYEVEPKADLYV
EECCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEE
YQNGEETTYAYVVNLNFLEPSPGNYYYFIEADSGKVLNKYNKLDHVANEDKSPVKQEAPK
EECCCCEEEEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHCCH
QEAKPAVKPVTGTNAVGTGKGVLGDTKSLNTTLSASSYYLQDNTRGATIFTYDAKNRSTL
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEECCCCCCEEEEEECCCCCCC
PGTLWVDADNVFNAAYDAAAVDAHYYAGKTYDYYKATFNRNSINDAGAPLKSTVHYGSRY
CEEEEEECCHHHHHHHHHHHHCCEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCCC
NNAFWNGSQMVYGDGDGVTFTSLSGGIDVIGHELTHAVTEYSSDLIYQNESGALNEAISD
CCCEECCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHH
VFGTLVEFYDNRNPDWEIGEDIYTPGKAGDALRSMSDPTKYGDPDHYSKRYTGTGDNGGV
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCE
HTNSGIINKAAYLLANGGTHYGVTVNGIGKDKVGAIYYRANTQYFTQSTTFSQARAGLVQ
ECCCCCHHCEEEEEECCCEEEEEEEECCCCCCCEEEEEEECCEEEECCCCHHHHHHHHHH
AATDLYGASSAEVAAVKQSYSAVGVN
HHHHHCCCCCCHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1495388; 3092843; 3127592; 1633827 [H]