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The map label for this gene is malK [H]

Identifier: 118476264

GI number: 118476264

Start: 596278

End: 597942

Strand: Direct

Name: malK [H]

Synonym: BALH_0516

Alternate gene names: 118476264

Gene position: 596278-597942 (Clockwise)

Preceding gene: 118476263

Following gene: 118476265

Centisome position: 11.34

GC content: 33.81

Gene sequence:

>1665_bases
GTGAGAATAATATCATCTTACTTATTTTTCTATTCAGCAATTAGGGGAGTCATTAACGGGATGAAAAAAAGGAAAAGACT
ATGGAATTTATGGAAAACGATTACATTGTTAGTTTGTACAGTTGTAATTTTTTCTTTACTTGTGACAGATATATTAATTA
GTCACAATGTAGAACGGACAACGGAGGATAGCCAAGCAGAGAAAGCAAAAACAATTGCACACATTGTGGCGAATGATTCA
ATTGTAATCGATGGTTTAATTGGGAAAGCAGATACTTCTGCAATTCAAACGTATACAAATAGAATATTAAAAAACACAGG
TGTTCAATTTATTGTAGTTATGGACATGAATGGAATAAGAAAGTCACATCCAAATCCTCAAAAAATAGGTCATCATTTTA
TTGGGGGAGATGAAGGACCTGCATTGAAAGGAAAGGAACATGTATCGCTGGCAGAAGGAACTTTAGGTATTTCAATGCGA
GTGTTTGTACCTGTATTTTCTGAAACAGGTGAACAACTTGGAGCGGTGGCTGTTGGTATTTTAGCAGATAATGTAAAAGA
GAGAGTTAAGGAAAGTAGACATATCATTTATATTGGAGTTGGAGTTGGGGTACTAGTCGGAATGATAGGAGCAATATTGC
TAGCTAGACATATAAAGAAAAGTTTATTCGGCCTTGAACCTCATAGGATAGCGAAAATTCTTGAAGAAAGAAATACGATG
CTACAATCTGTAAAGGAAGGTATTATTGCCGTAGATAAAGAGGCTAATGTAACTTTAATAAATAATGAGGCAAAACGATT
GTTTAAGAAAAGTGGATTGGAAGAAGATTTTATCGGTAAAGATGTTGAGTTGTATATGCCTAATTCACGTATAAAAGAAG
TATTACAAACGGGAGAGGTACAATTAAACGAAGAACAAAATATTTATGGAATTACGATTGTGACGAATCGTGTTCCTTTA
TATGTAAAAGGAGAAATAGTTGGCGCGATTGCAACATTCCGTGATAAAACAGAGATTAGAAAATTAGCAGAAGAATTAAC
TGGTATTAGGCTATATGCGGAAGCATTACGAGCACAATCGCATGAATTTATGAATAAAATGCATGTCGTATTAGGGCTTA
CACACATGAAACAGTATGAAGAATTACAGAAGTATATAAGCGGTATGGTATCAGAGCACCAATATGAAATTGGTGGGGTT
ATGAAAAGAATAAAAAGTCCAGTGTTTGCTGGTTTTTTACTCGGAAAACTGAGCTATGCTAGAGAGAAAAATATAAAATT
AATTATAAGTGAAGATTCTTACATGCCAGAAATAGATGATGAAAGTATTACTCATGAACTAATTACGATTGTCGGAAATT
TAATTGATAATGCATTAGAGGCAGTGACGAATTGTGAAAAGAAGCAAGTTGAAGTTGAGATACAATATGGGAATACATTA
ATTATTACAGTGCAAGATACGGGAAAAGGTATACAAGAAAAAGAAATAGAGGCATTATTTACGAAAGGTTATTCTACAAA
GGGAGATAACCGCGGTTATGGTTTGTATCTTGTAAAAGAAAGTATACAGCGAATAAATGGGGAAATTCATATGCATTCAT
TAGTAGGAAAGGGAACAAAGATAACAATTGAAATACCTAAAGGTAGGGATGAGAGGCAAATATGA

Upstream 100 bases:

>100_bases
GGAAGAAATTAGTTCAGCTTCGCAAAACTTAGCTGAGATGGCCGAAGAACTACAAGCGATGACTAGTAAATTTAAAGTAT
AATAACAGCAGATAAGTAAG

Downstream 100 bases:

>100_bases
TTAAAGTTTTAATTGTAGAAGATGACCCGATGGTAGCAATGTTAAATGCGCATTATTTAGAGCAAGTAGGAGGGTTTGAA
CTTGTTCAAGCAGTTAATTC

Product: sensory histidine kinase DcuS

Products: NA

Alternate protein names: Malate kinase sensor [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MRIISSYLFFYSAIRGVINGMKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDS
IVIDGLIGKADTSAIQTYTNRILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMR
VFVPVFSETGEQLGAVAVGILADNVKERVKESRHIIYIGVGVGVLVGMIGAILLARHIKKSLFGLEPHRIAKILEERNTM
LQSVKEGIIAVDKEANVTLINNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPL
YVKGEIVGAIATFRDKTEIRKLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGV
MKRIKSPVFAGFLLGKLSYAREKNIKLIISEDSYMPEIDDESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGNTL
IITVQDTGKGIQEKEIEALFTKGYSTKGDNRGYGLYLVKESIQRINGEIHMHSLVGKGTKITIEIPKGRDERQI

Sequences:

>Translated_554_residues
MRIISSYLFFYSAIRGVINGMKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDS
IVIDGLIGKADTSAIQTYTNRILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMR
VFVPVFSETGEQLGAVAVGILADNVKERVKESRHIIYIGVGVGVLVGMIGAILLARHIKKSLFGLEPHRIAKILEERNTM
LQSVKEGIIAVDKEANVTLINNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPL
YVKGEIVGAIATFRDKTEIRKLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGV
MKRIKSPVFAGFLLGKLSYAREKNIKLIISEDSYMPEIDDESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGNTL
IITVQDTGKGIQEKEIEALFTKGYSTKGDNRGYGLYLVKESIQRINGEIHMHSLVGKGTKITIEIPKGRDERQI
>Mature_554_residues
MRIISSYLFFYSAIRGVINGMKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERTTEDSQAEKAKTIAHIVANDS
IVIDGLIGKADTSAIQTYTNRILKNTGVQFIVVMDMNGIRKSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMR
VFVPVFSETGEQLGAVAVGILADNVKERVKESRHIIYIGVGVGVLVGMIGAILLARHIKKSLFGLEPHRIAKILEERNTM
LQSVKEGIIAVDKEANVTLINNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEVQLNEEQNIYGITIVTNRVPL
YVKGEIVGAIATFRDKTEIRKLAEELTGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGV
MKRIKSPVFAGFLLGKLSYAREKNIKLIISEDSYMPEIDDESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGNTL
IITVQDTGKGIQEKEIEALFTKGYSTKGDNRGYGLYLVKESIQRINGEIHMHSLVGKGTKITIEIPKGRDERQI

Specific function: Member of a two-component regulatory system malK/malR. Involved in the activation of maeA, maeN and yflS in presence of malate. Probably activates malR by phosphorylation [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=526, Percent_Identity=40.3041825095057, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1786837, Length=527, Percent_Identity=30.3605313092979, Blast_Score=246, Evalue=4e-66,
Organism=Escherichia coli, GI1788549, Length=355, Percent_Identity=21.9718309859155, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 61950; Mature: 61950

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIISSYLFFYSAIRGVINGMKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TEDSQAEKAKTIAHIVANDSIVIDGLIGKADTSAIQTYTNRILKNTGVQFIVVMDMNGIR
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCC
KSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGI
CCCCCHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEEEEHHHCCHHHHHHHHHH
LADNVKERVKESRHIIYIGVGVGVLVGMIGAILLARHIKKSLFGLEPHRIAKILEERNTM
HHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LQSVKEGIIAVDKEANVTLINNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEV
HHHHHCCCEEECCCCCEEEECHHHHHHHHHCCCCHHHCCCCEEEECCCHHHHHHHHCCCE
QLNEEQNIYGITIVTNRVPLYVKGEIVGAIATFRDKTEIRKLAEELTGIRLYAEALRAQS
EECCCCCEEEEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGVMKRIKSPVFAGFLLGKLSYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
REKNIKLIISEDSYMPEIDDESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGNTL
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEE
IITVQDTGKGIQEKEIEALFTKGYSTKGDNRGYGLYLVKESIQRINGEIHMHSLVGKGTK
EEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHCCCEEEEHHHCCCCE
ITIEIPKGRDERQI
EEEECCCCCCCCCC
>Mature Secondary Structure
MRIISSYLFFYSAIRGVINGMKKRKRLWNLWKTITLLVCTVVIFSLLVTDILISHNVERT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TEDSQAEKAKTIAHIVANDSIVIDGLIGKADTSAIQTYTNRILKNTGVQFIVVMDMNGIR
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCC
KSHPNPQKIGHHFIGGDEGPALKGKEHVSLAEGTLGISMRVFVPVFSETGEQLGAVAVGI
CCCCCHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEEEEHHHCCHHHHHHHHHH
LADNVKERVKESRHIIYIGVGVGVLVGMIGAILLARHIKKSLFGLEPHRIAKILEERNTM
HHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LQSVKEGIIAVDKEANVTLINNEAKRLFKKSGLEEDFIGKDVELYMPNSRIKEVLQTGEV
HHHHHCCCEEECCCCCEEEECHHHHHHHHHCCCCHHHCCCCEEEECCCHHHHHHHHCCCE
QLNEEQNIYGITIVTNRVPLYVKGEIVGAIATFRDKTEIRKLAEELTGIRLYAEALRAQS
EECCCCCEEEEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEFMNKMHVVLGLTHMKQYEELQKYISGMVSEHQYEIGGVMKRIKSPVFAGFLLGKLSYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
REKNIKLIISEDSYMPEIDDESITHELITIVGNLIDNALEAVTNCEKKQVEVEIQYGNTL
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEE
IITVQDTGKGIQEKEIEALFTKGYSTKGDNRGYGLYLVKESIQRINGEIHMHSLVGKGTK
EEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHCCCEEEEHHHCCCCE
ITIEIPKGRDERQI
EEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 12949159; 12949160 [H]