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The map label for this gene is citS [H]

Identifier: 118476247

GI number: 118476247

Start: 577767

End: 579377

Strand: Direct

Name: citS [H]

Synonym: BALH_0499

Alternate gene names: 118476247

Gene position: 577767-579377 (Clockwise)

Preceding gene: 118476246

Following gene: 118476248

Centisome position: 10.99

GC content: 34.51

Gene sequence:

>1611_bases
ATGGTTTTGAAATTTAAAAAATTAAAACTACAACCGAGAATTACATTAACTATTAGTGCACTTATTTTAGTTGTACTCCT
GTTAACGAGTTATTTATTTTATTACATATTATCTGAAACGGTAGAAGAGCAAATTGGCAAAAGAGCTTTGCATGTTGCGA
AAACAGTTGCTGCTATACCTGAAATAAAGGAAGCTTTTCAAAAAGAAAACCCAGCTTCTATTATTCAGCCGATTGCAGAA
AGAATTCGAATGGATACAGAGGCGGACTTCATTGTAGTTGGAAATAAAGAAGGGATTCGTTATGCACATCCTAGGAAAGA
TAAAATAGGAGAAGCAATGGTTGGCGGAGATAACAAAGGAGTTTTATTAGAAGGGAAATCTTACGTTTCGAAAGCAACTG
GATCATTAGGACCTTCATTACGCGGGAAAGTTCCAATCCGTAATCAGGAGAATGAAATTATTGGCGTGGTGTCTGTTGGA
TTTTCGATGGATGATATTCATGGAGCTGTAGAAGTGTATGGAAAACGTGTATTTTGGATTACAATAATAGGTCTTTTAAT
CGGGATAATTGGTTCTATATATTTAGCGGGAAGTATAAAAAGAATGATGTTTGGGATGGAGCCAGAAGAAATTTCATCTT
TATATGAAGAGCATAGTACAGTGATTCAATCTGTACGTGAAGGGATTATTGTTATTGATCAAAACGGCATGATTAGTTTA
GTCAATCAAGCAGCCTATGATATTCTTTCGCTAGATGAACAACGAAATATTATTGGAGAATTTATTTTAGATGTAATTCC
TAATTCAACGATATTAGATGTACTTCAAACTGGTGAAGAACAGTTTGATCGTCAATTAAATATAAAAGGACAGGCTGTTA
TTGCGAACCGTCTACCTATTAAAGTAAAGAATAAAGTAACTGGCGTTGTATCGAGCTTGCGTTTGAAATCTGAAATGGAT
CAGTTAACAGCAGAATTATCTCAGACGAAGCAATATACAGAGGCATTACGAGCGCAAACACATGAATATAACAATTTGTT
GTATACGCTTTCAGGTCTTGTCCAGCTAGAATTATATGAAGATGCTTTGGAGCTTATTCATAAGGAAACAGCGGTATATC
AAGATTTTGTTCAATTTATTATGAAACGAATTCAAAATCCATGGTTAGGTGGGATTTTAATTGGATTCTATAATAGAGCA
AGAGAGTTGAAGATTGATTTTATGCTAGATAGAGAAAGTAGCTTAGACAAATTAAGCCCGCATATTGAGAGTAATTATGT
CGTTTCTATTTTAGGAAATTTAATTACGAATGCATTTGAAGCGATTGAAAGAAATGAAGAACATAATAAGAAGGTAAGAA
TGTTTGTAACTGATATCGGAGAAGAAATAATAATTGAAGTGGAAGATTCAGGGCAAGGGGTTCATGATGAAATAATTACT
AGCATATTCTATAAAGGCTTCTCGACGAAAGAGGGCGGGAAGCGAGGATATGGGTTAGCAAAAGTGAAAGAGTTAGTAGA
AGATTTAAATGGAAGTATTGCAATCGAAAAAGGGGATTTAGGCGGCGCATTATTTATTATCGCATTACCAAAAGAGAGAG
GCGAATTGTAA

Upstream 100 bases:

>100_bases
GAAGAGAAAAAGACAGAAATATAGAGTAACCGTGTTATGATTAAGTATAATTGAAGAGACTGTATATTCTTTAAATTCTA
TCTTTAATTATGTTAGGGGA

Downstream 100 bases:

>100_bases
TGGGTGAGGAGATTGAAGTATTGATTGTAGAGGATGATATTCGAATTGCGGACATTCATCGTCGTTTTACTGAAAAGGTT
GAGGGGTTTAAAGTAATTGG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MVLKFKKLKLQPRITLTISALILVVLLLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEIKEAFQKENPASIIQPIAE
RIRMDTEADFIVVGNKEGIRYAHPRKDKIGEAMVGGDNKGVLLEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVG
FSMDDIHGAVEVYGKRVFWITIIGLLIGIIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQNGMISL
VNQAAYDILSLDEQRNIIGEFILDVIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKVKNKVTGVVSSLRLKSEMD
QLTAELSQTKQYTEALRAQTHEYNNLLYTLSGLVQLELYEDALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRA
RELKIDFMLDRESSLDKLSPHIESNYVVSILGNLITNAFEAIERNEEHNKKVRMFVTDIGEEIIIEVEDSGQGVHDEIIT
SIFYKGFSTKEGGKRGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGEL

Sequences:

>Translated_536_residues
MVLKFKKLKLQPRITLTISALILVVLLLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEIKEAFQKENPASIIQPIAE
RIRMDTEADFIVVGNKEGIRYAHPRKDKIGEAMVGGDNKGVLLEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVG
FSMDDIHGAVEVYGKRVFWITIIGLLIGIIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQNGMISL
VNQAAYDILSLDEQRNIIGEFILDVIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKVKNKVTGVVSSLRLKSEMD
QLTAELSQTKQYTEALRAQTHEYNNLLYTLSGLVQLELYEDALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRA
RELKIDFMLDRESSLDKLSPHIESNYVVSILGNLITNAFEAIERNEEHNKKVRMFVTDIGEEIIIEVEDSGQGVHDEIIT
SIFYKGFSTKEGGKRGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGEL
>Mature_536_residues
MVLKFKKLKLQPRITLTISALILVVLLLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEIKEAFQKENPASIIQPIAE
RIRMDTEADFIVVGNKEGIRYAHPRKDKIGEAMVGGDNKGVLLEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVG
FSMDDIHGAVEVYGKRVFWITIIGLLIGIIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQNGMISL
VNQAAYDILSLDEQRNIIGEFILDVIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKVKNKVTGVVSSLRLKSEMD
QLTAELSQTKQYTEALRAQTHEYNNLLYTLSGLVQLELYEDALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRA
RELKIDFMLDRESSLDKLSPHIESNYVVSILGNLITNAFEAIERNEEHNKKVRMFVTDIGEEIIIEVEDSGQGVHDEIIT
SIFYKGFSTKEGGKRGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGEL

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=534, Percent_Identity=32.0224719101124, Blast_Score=283, Evalue=3e-77,
Organism=Escherichia coli, GI1786837, Length=535, Percent_Identity=32.3364485981308, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI1788549, Length=343, Percent_Identity=23.3236151603499, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59902; Mature: 59902

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLKFKKLKLQPRITLTISALILVVLLLTSYLFYYILSETVEEQIGKRALHVAKTVAAIP
CCEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIKEAFQKENPASIIQPIAERIRMDTEADFIVVGNKEGIRYAHPRKDKIGEAMVGGDNKG
HHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHCCCHHCCCCCCC
VLLEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFSMDDIHGAVEVYGKRVFWI
EEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHH
TIIGLLIGIIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQNGMISL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHH
VNQAAYDILSLDEQRNIIGEFILDVIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPI
HHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCE
KVKNKVTGVVSSLRLKSEMDQLTAELSQTKQYTEALRAQTHEYNNLLYTLSGLVQLELYE
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARELKIDFMLDRESSLDKLSP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHCCC
HIESNYVVSILGNLITNAFEAIERNEEHNKKVRMFVTDIGEEIIIEVEDSGQGVHDEIIT
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEECCCCCCHHHHHHH
SIFYKGFSTKEGGKRGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGEL
HHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MVLKFKKLKLQPRITLTISALILVVLLLTSYLFYYILSETVEEQIGKRALHVAKTVAAIP
CCEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIKEAFQKENPASIIQPIAERIRMDTEADFIVVGNKEGIRYAHPRKDKIGEAMVGGDNKG
HHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHCCCHHCCCCCCC
VLLEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFSMDDIHGAVEVYGKRVFWI
EEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHH
TIIGLLIGIIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQNGMISL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHH
VNQAAYDILSLDEQRNIIGEFILDVIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPI
HHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCE
KVKNKVTGVVSSLRLKSEMDQLTAELSQTKQYTEALRAQTHEYNNLLYTLSGLVQLELYE
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARELKIDFMLDRESSLDKLSP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHCCC
HIESNYVVSILGNLITNAFEAIERNEEHNKKVRMFVTDIGEEIIIEVEDSGQGVHDEIIT
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEECCCCCCHHHHHHH
SIFYKGFSTKEGGKRGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGEL
HHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]