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The map label for this gene is pflB [H]

Identifier: 118476197

GI number: 118476197

Start: 505453

End: 507717

Strand: Direct

Name: pflB [H]

Synonym: BALH_0446

Alternate gene names: 118476197

Gene position: 505453-507717 (Clockwise)

Preceding gene: 118476196

Following gene: 118476198

Centisome position: 9.61

GC content: 36.07

Gene sequence:

>2265_bases
ATGGAGGAGATTAGGATGACTCAAGTATTAGAAAATGTTAAAAACGCATGGGAAAACTTTAAAGGTGAAAAATGGAAAGC
AGAGATTGATGTTCGCGATTTCATTTTAAATAATGTAAACGTTTACGAGGGAGACGAATCTTTCTTAGCGGGAGCAACTG
AAGCAACGAAACAACTTTGGGATCAAGTAATGGATTTAACAACGAAAGAACGTGAAAACGGTGGCGTTCTTGATATGGAT
ACAAAAATTGTTTCTTCTATTACATCACATGAACCAGGATATTTAAATAAAGAGACTGAAAAAGTAGTTGGTTTCCAAAC
TGATAAACCATTTAAACGTTCTTTACAACCATATGGTGGTATTCGTATGGCGGAACAAGCTTGTGAATCTTATGGATATG
AAATGGATAAAGAGCTTAGTCGTATTTTTAGAGATTGGAGAAAAACTCATAACCAAGGTGTATTTGATGCTTATACACCA
GAAATGAGAGCTGCTCGTAAATCAGGTGTTATTACGGGTCTTCCAGATGCATACGGACGTGGACGTATTATCGGTGACTA
CCGCCGCGTAGCATTATATGGAGTAGATCATTTAATTGAAGCGAAGAAAACAGATTTAAATTTAACTGGTGGTGTAATGA
GTGAAGATACAATGCGTTTACGCGAAGAGTTATCTGAGCAAATACGTGCACTTCAAGAGTTAAAACAAATGGCTGCTTCT
CATGGCTTTGATATTTCTAAGCCAGCAACAACTGCTCAAGAAGCATTCCAATGGTTATACTTTGCATATCTTGCAGCAAT
TAAAGAGCAAAACGGAGCTGCAATGAGTCTTGGACGTACTTCTACATTCTTAGATATTTATATTGAAAGAGATTTAGCAA
ATGGTACTTTAACAGAAGAAGACGTACAAGAAATTGTGGATCACTTCATTATGAAATTACGTCTTGTGAAATTTGCAAGA
ACACCTGATTATAATGAGTTATTCTCTGGTGACCCAACTTGGGTAACTGAGTCTATCGGTGGTATGGCATTAGATGGTCG
TCCATTAGTAACAAAGAACTCATTCCGTTTCTTGCATACATTAGATAATTTAGGACCAGCTCCAGAACCAAACTTAACAG
TTCTTTGGTCTAAACAATTACCACAGAACTTTAAAAACTACTGTGCGAAAATGTCTATTAAAACATCAGCAATTCAATAT
GAAAATGATGACATTATGCGTGCTGACTACGGCGATGACTACGGAATTGCTTGTTGTGTATCTGCAATGAGAATCGGTAA
ACAAATGCAATTCTTTGGCGCACGTGCAAACTTAGCAAAAGCATTACTATATGCGATTAACGGTGGTAAAGATGAAAAAT
CTAAAGCACAAGTTGGTCCTGAGTATGCACCGATTACTTCTGAAGTATTAGATTATGAAGAAGTTATGCATAAGTTTGAT
ATGACAATGGAATGGTTAGCGGGTCTATATTTAAACACATTAAATGTTATTCACTACATGCACGATAAATATAGCTACGA
ACGTATTGAAATGGCACTTCATGATACAAATGTTCTTCGTACAATGGCAACAGGCATTGCGGGTCTATCTGTAGTAGCAG
ACTCTTTAAGTGCAATTAAATATGCAAAAGTAAAACCAATTCGCGATGAAAACGGAATTGCAGTTGACTTTGAAATTGAA
GGAGATTTCCCTAAATACGGTAACAATGATGACCGTGTAGATGAAATTGCTGTAAATCTTGTGAAAACATTTATGAATAA
ACTTCGTAAACATAAAACATACAGAAATTCTGTTCATACAATGTCAATCTTAACAATCACATCTAACGTTGTATACGGTA
AGAAAACTGGTAACACTCCAGATGGTCGCCGTACTGGAGAACCATTTGCACCAGGTGCGAACCCAATGCATGGACGTGAT
ACAAAAGGTGCGTTAGCTTCATTATTATCTGTAGCTAAATTACCATATGAAGATGCACAAGATGGTATTTCTAATACATT
CTCTATTATTCCAAAAGCGCTTGGTAAAGAGGATGAAGTACAAGTACGTAACTTAGTATCAATGCTTGATGGTTATGCAG
TAAAAGAAGGCCATCACTTAAATATTAACGTATTTAATCGTGAAACATTAATGGATGCAATGGAACATCCTGAGAAATAT
CCACAATTAACAATTCGCGTATCTGGTTACGCTGTAAACTTTATTAAATTAACTCGTGAACAACAAATCGATGTAATTAA
CCGTACAATGCATGAAAGCATGTAA

Upstream 100 bases:

>100_bases
CACAAACATGTCACAACTATTTCGTTCACTTTCGTAAAAAAGTAGTATTCTAAGTGTGTAAGCTGTTATATAAAAAATCT
TAGTCCTGTACTAATCAAAA

Downstream 100 bases:

>100_bases
ATGAAAAAAGTGTCCCTCTGCATTTTATAAACAGCAGAGGGAGGCTGTTTCAATAAAGGAGGAAGTAACATGGTAAAAGG
AAGAATTCATTCTGTAGAGT

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 754; Mature: 754

Protein sequence:

>754_residues
MEEIRMTQVLENVKNAWENFKGEKWKAEIDVRDFILNNVNVYEGDESFLAGATEATKQLWDQVMDLTTKERENGGVLDMD
TKIVSSITSHEPGYLNKETEKVVGFQTDKPFKRSLQPYGGIRMAEQACESYGYEMDKELSRIFRDWRKTHNQGVFDAYTP
EMRAARKSGVITGLPDAYGRGRIIGDYRRVALYGVDHLIEAKKTDLNLTGGVMSEDTMRLREELSEQIRALQELKQMAAS
HGFDISKPATTAQEAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYIERDLANGTLTEEDVQEIVDHFIMKLRLVKFAR
TPDYNELFSGDPTWVTESIGGMALDGRPLVTKNSFRFLHTLDNLGPAPEPNLTVLWSKQLPQNFKNYCAKMSIKTSAIQY
ENDDIMRADYGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGKDEKSKAQVGPEYAPITSEVLDYEEVMHKFD
MTMEWLAGLYLNTLNVIHYMHDKYSYERIEMALHDTNVLRTMATGIAGLSVVADSLSAIKYAKVKPIRDENGIAVDFEIE
GDFPKYGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILTITSNVVYGKKTGNTPDGRRTGEPFAPGANPMHGRD
TKGALASLLSVAKLPYEDAQDGISNTFSIIPKALGKEDEVQVRNLVSMLDGYAVKEGHHLNINVFNRETLMDAMEHPEKY
PQLTIRVSGYAVNFIKLTREQQIDVINRTMHESM

Sequences:

>Translated_754_residues
MEEIRMTQVLENVKNAWENFKGEKWKAEIDVRDFILNNVNVYEGDESFLAGATEATKQLWDQVMDLTTKERENGGVLDMD
TKIVSSITSHEPGYLNKETEKVVGFQTDKPFKRSLQPYGGIRMAEQACESYGYEMDKELSRIFRDWRKTHNQGVFDAYTP
EMRAARKSGVITGLPDAYGRGRIIGDYRRVALYGVDHLIEAKKTDLNLTGGVMSEDTMRLREELSEQIRALQELKQMAAS
HGFDISKPATTAQEAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYIERDLANGTLTEEDVQEIVDHFIMKLRLVKFAR
TPDYNELFSGDPTWVTESIGGMALDGRPLVTKNSFRFLHTLDNLGPAPEPNLTVLWSKQLPQNFKNYCAKMSIKTSAIQY
ENDDIMRADYGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGKDEKSKAQVGPEYAPITSEVLDYEEVMHKFD
MTMEWLAGLYLNTLNVIHYMHDKYSYERIEMALHDTNVLRTMATGIAGLSVVADSLSAIKYAKVKPIRDENGIAVDFEIE
GDFPKYGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILTITSNVVYGKKTGNTPDGRRTGEPFAPGANPMHGRD
TKGALASLLSVAKLPYEDAQDGISNTFSIIPKALGKEDEVQVRNLVSMLDGYAVKEGHHLNINVFNRETLMDAMEHPEKY
PQLTIRVSGYAVNFIKLTREQQIDVINRTMHESM
>Mature_754_residues
MEEIRMTQVLENVKNAWENFKGEKWKAEIDVRDFILNNVNVYEGDESFLAGATEATKQLWDQVMDLTTKERENGGVLDMD
TKIVSSITSHEPGYLNKETEKVVGFQTDKPFKRSLQPYGGIRMAEQACESYGYEMDKELSRIFRDWRKTHNQGVFDAYTP
EMRAARKSGVITGLPDAYGRGRIIGDYRRVALYGVDHLIEAKKTDLNLTGGVMSEDTMRLREELSEQIRALQELKQMAAS
HGFDISKPATTAQEAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYIERDLANGTLTEEDVQEIVDHFIMKLRLVKFAR
TPDYNELFSGDPTWVTESIGGMALDGRPLVTKNSFRFLHTLDNLGPAPEPNLTVLWSKQLPQNFKNYCAKMSIKTSAIQY
ENDDIMRADYGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGKDEKSKAQVGPEYAPITSEVLDYEEVMHKFD
MTMEWLAGLYLNTLNVIHYMHDKYSYERIEMALHDTNVLRTMATGIAGLSVVADSLSAIKYAKVKPIRDENGIAVDFEIE
GDFPKYGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILTITSNVVYGKKTGNTPDGRRTGEPFAPGANPMHGRD
TKGALASLLSVAKLPYEDAQDGISNTFSIIPKALGKEDEVQVRNLVSMLDGYAVKEGHHLNINVFNRETLMDAMEHPEKY
PQLTIRVSGYAVNFIKLTREQQIDVINRTMHESM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=760, Percent_Identity=65, Blast_Score=1053, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=751, Percent_Identity=65.5126498002663, Blast_Score=1027, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=574, Percent_Identity=27.3519163763066, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1790388, Length=698, Percent_Identity=25.214899713467, Blast_Score=156, Evalue=5e-39,
Organism=Escherichia coli, GI1788933, Length=61, Percent_Identity=70.4918032786885, Blast_Score=90, Evalue=5e-19,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 85215; Mature: 85215

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEIRMTQVLENVKNAWENFKGEKWKAEIDVRDFILNNVNVYEGDESFLAGATEATKQLW
CCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCEEEECCHHHHCCHHHHHHHHH
DQVMDLTTKERENGGVLDMDTKIVSSITSHEPGYLNKETEKVVGFQTDKPFKRSLQPYGG
HHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCHHHCCCCCCC
IRMAEQACESYGYEMDKELSRIFRDWRKTHNQGVFDAYTPEMRAARKSGVITGLPDAYGR
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEECCCCCCCC
GRIIGDYRRVALYGVDHLIEAKKTDLNLTGGVMSEDTMRLREELSEQIRALQELKQMAAS
CCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HGFDISKPATTAQEAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYIERDLANGTLTEE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEEEECCCCCCCCHH
DVQEIVDHFIMKLRLVKFARTPDYNELFSGDPTWVTESIGGMALDGRPLVTKNSFRFLHT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHCCCCEEECCCCEEECCCHHHHHH
LDNLGPAPEPNLTVLWSKQLPQNFKNYCAKMSIKTSAIQYENDDIMRADYGDDYGIACCV
HHHCCCCCCCCEEEEECHHCHHHHHHHHHHCCEEHHEEEECCCCEEECCCCCCCHHHHHH
SAMRIGKQMQFFGARANLAKALLYAINGGKDEKSKAQVGPEYAPITSEVLDYEEVMHKFD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHH
MTMEWLAGLYLNTLNVIHYMHDKYSYERIEMALHDTNVLRTMATGIAGLSVVADSLSAIK
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAKVKPIRDENGIAVDFEIEGDFPKYGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHT
HHHCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHE
MSILTITSNVVYGKKTGNTPDGRRTGEPFAPGANPMHGRDTKGALASLLSVAKLPYEDAQ
EEEEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
DGISNTFSIIPKALGKEDEVQVRNLVSMLDGYAVKEGHHLNINVFNRETLMDAMEHPEKY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEECCCCEEEEEEECHHHHHHHHHCCCCC
PQLTIRVSGYAVNFIKLTREQQIDVINRTMHESM
CEEEEEEECEEEEEEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEEIRMTQVLENVKNAWENFKGEKWKAEIDVRDFILNNVNVYEGDESFLAGATEATKQLW
CCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCEEEECCHHHHCCHHHHHHHHH
DQVMDLTTKERENGGVLDMDTKIVSSITSHEPGYLNKETEKVVGFQTDKPFKRSLQPYGG
HHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCHHHCCCCCCC
IRMAEQACESYGYEMDKELSRIFRDWRKTHNQGVFDAYTPEMRAARKSGVITGLPDAYGR
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEECCCCCCCC
GRIIGDYRRVALYGVDHLIEAKKTDLNLTGGVMSEDTMRLREELSEQIRALQELKQMAAS
CCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HGFDISKPATTAQEAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYIERDLANGTLTEE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEEEECCCCCCCCHH
DVQEIVDHFIMKLRLVKFARTPDYNELFSGDPTWVTESIGGMALDGRPLVTKNSFRFLHT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHCCCCEEECCCCEEECCCHHHHHH
LDNLGPAPEPNLTVLWSKQLPQNFKNYCAKMSIKTSAIQYENDDIMRADYGDDYGIACCV
HHHCCCCCCCCEEEEECHHCHHHHHHHHHHCCEEHHEEEECCCCEEECCCCCCCHHHHHH
SAMRIGKQMQFFGARANLAKALLYAINGGKDEKSKAQVGPEYAPITSEVLDYEEVMHKFD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHH
MTMEWLAGLYLNTLNVIHYMHDKYSYERIEMALHDTNVLRTMATGIAGLSVVADSLSAIK
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAKVKPIRDENGIAVDFEIEGDFPKYGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHT
HHHCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHE
MSILTITSNVVYGKKTGNTPDGRRTGEPFAPGANPMHGRDTKGALASLLSVAKLPYEDAQ
EEEEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
DGISNTFSIIPKALGKEDEVQVRNLVSMLDGYAVKEGHHLNINVFNRETLMDAMEHPEKY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEECCCCEEEEEEECHHHHHHHHHCCCCC
PQLTIRVSGYAVNFIKLTREQQIDVINRTMHESM
CEEEEEEECEEEEEEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA