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The map label for this gene is truA [H]

Identifier: 118476179

GI number: 118476179

Start: 486516

End: 487268

Strand: Direct

Name: truA [H]

Synonym: BALH_0426

Alternate gene names: 118476179

Gene position: 486516-487268 (Clockwise)

Preceding gene: 118476178

Following gene: 118476181

Centisome position: 9.25

GC content: 34.53

Gene sequence:

>753_bases
GTGAAGGAGAATAAGATGAACAATTATAAATTAACAATTCAGTACGACGGTGCACGTTTTAAAGGGTGGCAACGTCTCGG
CAATAACGATAACACGATTCAAGGGAAAATCGAAAGTGTCATATCTGAAATGATCGGAAAAGAAATTGAAATTATCGGTT
GCAGTAGAACAGACGCTGGTGTACATGCTTTGAATCAAGTGGCTAACTTTCAAAGTGATGAGAAGTTAGTAGAACATAAA
GTGAAAAAATATTTAAATCAATATTTACCAAATGATATTAGCATTACGAATGTAGAAGAAGTACATGATCGCTTCCATGC
TCGTTACAATTCTAAAGCAAAAACATATCTGTATAAAATTTGGAATGAAGAGCATACGAATCCATTTATGCGTAAATACA
GCATGCATGTGAATAAAAAATTAAATGTGAAAAGCATGAAAGAAGCTGCGAAACATTTAGTTGGTTCACATGACTTTACT
GCTTTTTCAAATGCAAAATCAAAGAAAAAGTCTATGGTACGAGAAGTATATTCACTTGAAGTAATGGAAGAGGCAGGATT
TGTACAAATTAGAGTAAGTGGCAACGGATTTCTTCATAACATGGTACGAAAAATTGTTGGAGCATTAATTGAAGTTGGAT
TAGGACAATTAGCTGCAGAGGCGATTCCACAAATTCTAGAGGCGAAACAACGTAATCAAATTAATTGCCTTGCTGAGGCG
AGTGGTTTGTATTTGGAGAATGTTGAGTTTTAA

Upstream 100 bases:

>100_bases
TTGATATGTTCCCGCACACAGCTCATGTAGAAAATGTAGTGAAGCTTGTTAGAAAGTAAAGTTTATTCATATTCCCTCAT
GATATGAGGGAATATTTTCT

Downstream 100 bases:

>100_bases
TAATCAGTAAAAAAATCGAGCTGTGTAGGCTCGATTTTTTTATGTTTTGGCGTTAAGAAGATTTCTCTCTCTTCATACGT
ATCTTCAAATTCCAAAAAGC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 2; tRNA-uridine isomerase I 2 [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MKENKMNNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMIGKEIEIIGCSRTDAGVHALNQVANFQSDEKLVEHK
VKKYLNQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEHTNPFMRKYSMHVNKKLNVKSMKEAAKHLVGSHDFT
AFSNAKSKKKSMVREVYSLEVMEEAGFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLAAEAIPQILEAKQRNQINCLAEA
SGLYLENVEF

Sequences:

>Translated_250_residues
MKENKMNNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMIGKEIEIIGCSRTDAGVHALNQVANFQSDEKLVEHK
VKKYLNQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEHTNPFMRKYSMHVNKKLNVKSMKEAAKHLVGSHDFT
AFSNAKSKKKSMVREVYSLEVMEEAGFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLAAEAIPQILEAKQRNQINCLAEA
SGLYLENVEF
>Mature_250_residues
MKENKMNNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMIGKEIEIIGCSRTDAGVHALNQVANFQSDEKLVEHK
VKKYLNQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKIWNEEHTNPFMRKYSMHVNKKLNVKSMKEAAKHLVGSHDFT
AFSNAKSKKKSMVREVYSLEVMEEAGFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLAAEAIPQILEAKQRNQINCLAEA
SGLYLENVEF

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=280, Percent_Identity=28.9285714285714, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788657, Length=240, Percent_Identity=34.5833333333333, Blast_Score=157, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI71985046, Length=266, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28585; Mature: 28585

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENKMNNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMIGKEIEIIGCSRTDAG
CCCCCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHH
VHALNQVANFQSDEKLVEHKVKKYLNQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHEE
WNEEHTNPFMRKYSMHVNKKLNVKSMKEAAKHLVGSHDFTAFSNAKSKKKSMVREVYSLE
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VMEEAGFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLAAEAIPQILEAKQRNQINCLAEA
HHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHC
SGLYLENVEF
CCCEEECCCC
>Mature Secondary Structure
MKENKMNNYKLTIQYDGARFKGWQRLGNNDNTIQGKIESVISEMIGKEIEIIGCSRTDAG
CCCCCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHH
VHALNQVANFQSDEKLVEHKVKKYLNQYLPNDISITNVEEVHDRFHARYNSKAKTYLYKI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHEE
WNEEHTNPFMRKYSMHVNKKLNVKSMKEAAKHLVGSHDFTAFSNAKSKKKSMVREVYSLE
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VMEEAGFVQIRVSGNGFLHNMVRKIVGALIEVGLGQLAAEAIPQILEAKQRNQINCLAEA
HHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHC
SGLYLENVEF
CCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]