Click here to switch to the map view.

The map label for this gene is purD [H]

Identifier: 118476050

GI number: 118476050

Start: 336652

End: 337923

Strand: Direct

Name: purD [H]

Synonym: BALH_0293

Alternate gene names: 118476050

Gene position: 336652-337923 (Clockwise)

Preceding gene: 118476049

Following gene: 118476053

Centisome position: 6.4

GC content: 38.29

Gene sequence:

>1272_bases
ATGAATGTTTTAGTAATTGGCCGCGGTGGGCGTGAGCATGCTTTAGCTTGGAAGTTTGCACAATCTGAAAAAGTAGAGAA
GGTATATGTAGCACCAGGTAATGAAGGTATGCGTGATGTTGCAACACCAATTGATATTGATGAAAATGATTTTGATGCAT
TAATCTTATTTGCGAAAGAGAATCATGTTGAGTTAACTTTCGTTGGACCAGAAATTCCACTTATGAATGGAATTGTTGAT
CGTTTTAAAGAAGAGGGACTTCGCGTATTTGGTCCGAATAAAGCAGCTGCTGTTATTGAAGGTAGTAAAGCTTTTACAAA
AGAATTGATGAAAAAGTATAATATTCCAACTGCGGCGTACGAGACTTTTACAGATTATGAAGAAGCAGTACAGTACATTG
AAAAAGTTGGTGCACCAATTGTTATTAAAGCGGATGGTCTAGCTGCTGGTAAAGGTGTAACAGTAGCGATGACGCTTGAA
GAGGCATTACAAGCTGCGAAAGAAATGCTGCAAGATGTAAAGTTCGGGGAAGCGAGTAAGAAAGTTGTTATTGAAGAGTT
TTTAGATGGACAAGAATTTTCATTAATGGCATTTGTGAATGGAACAACTGTACATCCGATGGTAATTGCGCAAGATCATA
AACGAGCTTTTGATGGTGATAAAGGTCCAAATACTGGCGGAATGGGTGCATATTCTCCAGTACCACAAATTTCGGAGTCA
GCAGTTCAAGAGGCGATTGAAACGGTGTTACATCCAACTGCTAAAGCGATGATCCAAGAAAATCGATCGTTTACAGGAAT
TTTATATGCGGGACTTATTTTAACAAATGATGGTCCAAAGGTAATTGAATTTAATGCACGTTTTGGCGATCCTGAAACTG
AAGTTGTATTACCCCGTTTAGAAAATGATTTAGTGGATGTATGTAACGCTGTATTAGATGAAAGTGAGTTAACGTTACAA
TGGTCAGAGGAATCGGTAATTGGTGTTGTGCTTGCCTCCAAAGGATATCCGGAAGCATATAAAAAAGGTGACATTATTAA
AGGGCTAGATGCATTGCAAGATGTAATTGTTTTCCATGCGGGTACAGCAATGAAACATGGTGACTTTGTGACAAACGGTG
GCCGTGTGCTATTTGTTGCTTGTAAGGCGAATAGTTTACAAGAAGCAAAAGATACAGTATATAAAGAAATCGGTAAAATT
GAGAGTGATGGTTTATTTTACCGAAGTGATATAGGATATCGTGCAATTGGGCATGAGATGACGAGAAATTAA

Upstream 100 bases:

>100_bases
CGGTTAAGATCACCTTCCTTTTTAACTTTGATTGATATGAAAAAACAAACTAGGAGCATAGCTTCTAGTTTGTACTTATT
TAAATATTGGGGGATGAAAT

Downstream 100 bases:

>100_bases
TATGTAAAAAATCCCGCTGATTCCTCAGCGGGATTTTTTATGCATCTTTATCCTTTTTTAATTTCCCTTTTTGCTTTGCC
ATTTCTCGAAGTAAATATTC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MNVLVIGRGGREHALAWKFAQSEKVEKVYVAPGNEGMRDVATPIDIDENDFDALILFAKENHVELTFVGPEIPLMNGIVD
RFKEEGLRVFGPNKAAAVIEGSKAFTKELMKKYNIPTAAYETFTDYEEAVQYIEKVGAPIVIKADGLAAGKGVTVAMTLE
EALQAAKEMLQDVKFGEASKKVVIEEFLDGQEFSLMAFVNGTTVHPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQISES
AVQEAIETVLHPTAKAMIQENRSFTGILYAGLILTNDGPKVIEFNARFGDPETEVVLPRLENDLVDVCNAVLDESELTLQ
WSEESVIGVVLASKGYPEAYKKGDIIKGLDALQDVIVFHAGTAMKHGDFVTNGGRVLFVACKANSLQEAKDTVYKEIGKI
ESDGLFYRSDIGYRAIGHEMTRN

Sequences:

>Translated_423_residues
MNVLVIGRGGREHALAWKFAQSEKVEKVYVAPGNEGMRDVATPIDIDENDFDALILFAKENHVELTFVGPEIPLMNGIVD
RFKEEGLRVFGPNKAAAVIEGSKAFTKELMKKYNIPTAAYETFTDYEEAVQYIEKVGAPIVIKADGLAAGKGVTVAMTLE
EALQAAKEMLQDVKFGEASKKVVIEEFLDGQEFSLMAFVNGTTVHPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQISES
AVQEAIETVLHPTAKAMIQENRSFTGILYAGLILTNDGPKVIEFNARFGDPETEVVLPRLENDLVDVCNAVLDESELTLQ
WSEESVIGVVLASKGYPEAYKKGDIIKGLDALQDVIVFHAGTAMKHGDFVTNGGRVLFVACKANSLQEAKDTVYKEIGKI
ESDGLFYRSDIGYRAIGHEMTRN
>Mature_423_residues
MNVLVIGRGGREHALAWKFAQSEKVEKVYVAPGNEGMRDVATPIDIDENDFDALILFAKENHVELTFVGPEIPLMNGIVD
RFKEEGLRVFGPNKAAAVIEGSKAFTKELMKKYNIPTAAYETFTDYEEAVQYIEKVGAPIVIKADGLAAGKGVTVAMTLE
EALQAAKEMLQDVKFGEASKKVVIEEFLDGQEFSLMAFVNGTTVHPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQISES
AVQEAIETVLHPTAKAMIQENRSFTGILYAGLILTNDGPKVIEFNARFGDPETEVVLPRLENDLVDVCNAVLDESELTLQ
WSEESVIGVVLASKGYPEAYKKGDIIKGLDALQDVIVFHAGTAMKHGDFVTNGGRVLFVACKANSLQEAKDTVYKEIGKI
ESDGLFYRSDIGYRAIGHEMTRN

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=48.6997635933806, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=48.6997635933806, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=48.6997635933806, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=48.6997635933806, Blast_Score=389, Evalue=1e-108,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=51.4018691588785, Blast_Score=422, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17567511, Length=422, Percent_Identity=41.7061611374408, Blast_Score=310, Evalue=9e-85,
Organism=Saccharomyces cerevisiae, GI6321203, Length=439, Percent_Identity=43.5079726651481, Blast_Score=332, Evalue=6e-92,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=47.4418604651163, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=47.4418604651163, Blast_Score=357, Evalue=6e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46188; Mature: 46188

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVIGRGGREHALAWKFAQSEKVEKVYVAPGNEGMRDVATPIDIDENDFDALILFAKE
CEEEEEECCCCCCCEEEEECCCCCCCEEEECCCCCCHHHHCCCCCCCCCCCCEEEEEEEC
NHVELTFVGPEIPLMNGIVDRFKEEGLRVFGPNKAAAVIEGSKAFTKELMKKYNIPTAAY
CCEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHCCCHHHH
ETFTDYEEAVQYIEKVGAPIVIKADGLAAGKGVTVAMTLEEALQAAKEMLQDVKFGEASK
HHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHH
KVVIEEFLDGQEFSLMAFVNGTTVHPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQISES
HHHHHHHCCCCCEEEEEEECCCEECEEEEECCCHHHCCCCCCCCCCCCCCCCCCCHHHHH
AVQEAIETVLHPTAKAMIQENRSFTGILYAGLILTNDGPKVIEFNARFGDPETEVVLPRL
HHHHHHHHHHCHHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCEEECCHH
ENDLVDVCNAVLDESELTLQWSEESVIGVVLASKGYPEAYKKGDIIKGLDALQDVIVFHA
HHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHCC
GTAMKHGDFVTNGGRVLFVACKANSLQEAKDTVYKEIGKIESDGLFYRSDIGYRAIGHEM
CCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHCHHH
TRN
CCC
>Mature Secondary Structure
MNVLVIGRGGREHALAWKFAQSEKVEKVYVAPGNEGMRDVATPIDIDENDFDALILFAKE
CEEEEEECCCCCCCEEEEECCCCCCCEEEECCCCCCHHHHCCCCCCCCCCCCEEEEEEEC
NHVELTFVGPEIPLMNGIVDRFKEEGLRVFGPNKAAAVIEGSKAFTKELMKKYNIPTAAY
CCEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHCCCHHHH
ETFTDYEEAVQYIEKVGAPIVIKADGLAAGKGVTVAMTLEEALQAAKEMLQDVKFGEASK
HHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHH
KVVIEEFLDGQEFSLMAFVNGTTVHPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQISES
HHHHHHHCCCCCEEEEEEECCCEECEEEEECCCHHHCCCCCCCCCCCCCCCCCCCHHHHH
AVQEAIETVLHPTAKAMIQENRSFTGILYAGLILTNDGPKVIEFNARFGDPETEVVLPRL
HHHHHHHHHHCHHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCEEECCHH
ENDLVDVCNAVLDESELTLQWSEESVIGVVLASKGYPEAYKKGDIIKGLDALQDVIVFHA
HHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHCC
GTAMKHGDFVTNGGRVLFVACKANSLQEAKDTVYKEIGKIESDGLFYRSDIGYRAIGHEM
CCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHCHHH
TRN
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]