Click here to switch to the map view.

The map label for this gene is rlmL

Identifier: 117920049

GI number: 117920049

Start: 1878599

End: 1880740

Strand: Direct

Name: rlmL

Synonym: Shewana3_1601

Alternate gene names: 117920049

Gene position: 1878599-1880740 (Clockwise)

Preceding gene: 117920044

Following gene: 117920050

Centisome position: 37.78

GC content: 48.74

Gene sequence:

>2142_bases
ATGCTTAATTTTTTTGCTGCCGCCCCTAAGGGCTTTGAATATAGCTTAGCCCAAGAACTGACAGAATTTGGTGCGACTGA
GATTAAAGAGAGTGTTGCCGGTGTCTATTTTACCGCGCCGCTTGCCTTAGCCTACCGTATTACCCTGTGGACGCGTTTGG
CCAGCCGTATCGTGCTGGTGATCTATAAGGGCCCCTGTGAGTCGGCGGAGCAATTATATAACGCAGCTTATTGTATTGAT
TGGTCAGCTCATTTCTCCAATCGCAACACCTTTAGTATCGATTTTCACGGCACTGGCGGCTTTATCAACAACACCCAGTT
TGGTGCGTTAAAGATTAAAGATGCGATTGTTGACCGTTTCCGTGATGACGGTGATGCCCGTCCAAACGTCGCCCGTATCG
ATGCCGACATTAAAATCGACGCCCATTTCCGTAATGGGGTGATTACCATTGCGATGAACTTCTCGGGACCTTCTTTGCAT
CAACGCGGTTATCGCTCTACGACCGGTGAAGCACCGTTAAAAGAAAACCTCGCCGCCAACATGCTAGTGCGCAGTGGCTG
GAAAGCCGCGCCAACGACCTTACTCGATCCTTTCTGCGGCAGTGGTACTGTACTGATTGAGGCCGCATTAATGGCTGCCG
ATATTGCTCCAGGGTTGCAGCGCAGCCGTTTTGGTTTTGAGCATTGGCGCCGCCATGATAAAGCGACATGGCATGAGATT
TTAGAAGAGGCCAAGGCGCGCGCATCCTTAGGTGTAAAGCGTTGTGACGTGAAGTTCTACGGCTCGGATATCGATTCGCG
CTTAGTGGCCCTAGCAAAACGCAATGCGCAAAACGCCGGTGTATTTGAGCTGATTGACTTTAAAGTCGCTAATGCGCTCA
ATGTCGAGCCGCCCGCCGCCGAAGGTTACCTGATTACTAACCCTCCCTACGGTGAGCGTTTAGGCAGTGTTTCTGAGCTA
TTGCAGCTGTATTACCAATTAGGCGACAAGTTTAAAAAGGAGTTTGGCGGCTGGAAAGTGGCTATGCTCTGTAGCGATAT
TGAGCTGATTTCGGCGCTGAAACTCAAAGCCGATAAACAGATGAAGATGTTTAATGGTGCGCTGGAGTGTGCCTTTAACC
TGTATACCTTGCACGCGCAAAGCACTCGCCGAGATACGCCAGTATTGCCGGAAGGAGTAGACATTGCCGATATCGCGCCA
GCATTTGCTAACCGTATTAAGAAAAACGCCAAGCAATTAGAAAAGTGGGCGAAAAAAGAAGGTATCGATAGCTACCGTTT
ATATGATGCCGACATTCCCGAATATAACGTGGCGGTCGACCGCTACTTAGATCATATCGTGGTGCAGGAATATATGGCGC
CGGCCAGCATTCCTGAGGCCGTGACAAAACGTCGTTTAAGCGATGTGCTCTTGGCGCTGCCTGCTGCGATTGGTGTCGAT
CCGCACAAGATCACTATGAAGACCCGTGAGCGTCAAAAGGGCACCAATCAATATCAAAAGCTCGATGAGCGTAAGCTTGA
GCTTATCACCACCGAATATGGCGCTAAGTTTAAGCTGAACCTGACAGGCTATTTGGATACTGGCTTGTTCCTCGATCACA
GATTAACCCGCCGTTTAGTGGGACAAAAATCGAAAGGTCGCCGGGTATTGAACCTGTTCTCCTACACAGGTTCTGCCTCT
GTGCATGCCGCATTAGGCGGTGCTAAGTCGGTCACGACGGTTGATATGTCCAACACTTATCTTGCGTGGGCAAAGGAAAA
CTTTGCGTTAAACGATCTCAGTGGCAAGCAGTATGAGTTTGTGCAGGCCGATTGTTTGCAATGGATCCGCGATAGTGCGC
ACGATAAATCCGCGCAATACGATTTGATTTTTATCGATCCGCCGACCTTCTCTAACTCTAAGCGGATGGAAGATTCCTTC
GATGTACAGCGTGACCATGTGAATTTACTAGGCATGCTTATCAAGCTGTTAAGTCCAAATGGGGAGATAGTGTTCTCCAA
CAACAAACGCAAGTTTAAGATGGACACCGACACCTTGGTCAAGATGAAAATCAAGGTGGAAAACATCGATGATCTGACCT
TGCCTATGGATTACAAGCGCAATCCCCATATCCATAATACTTGGTTAATCACCCATGCATAA

Upstream 100 bases:

>100_bases
ACTGCCTGACTAAATCGAGTTTCGGCTAATAACTGTTTATCTTTTATGTCGCTTCACAGGTATAATGTCGCTTTGATTTT
GACTGCGTATTGATTATCCC

Downstream 100 bases:

>100_bases
TGTACCGCAAGCGGGTTACATACTATACCACACCGAAGGCTGTCACTTATGTGAACAGGCTGCGGCATTGCTTAATGCCG
CCGAGGTGCCTTTTACGGCC

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 713; Mature: 713

Protein sequence:

>713_residues
MLNFFAAAPKGFEYSLAQELTEFGATEIKESVAGVYFTAPLALAYRITLWTRLASRIVLVIYKGPCESAEQLYNAAYCID
WSAHFSNRNTFSIDFHGTGGFINNTQFGALKIKDAIVDRFRDDGDARPNVARIDADIKIDAHFRNGVITIAMNFSGPSLH
QRGYRSTTGEAPLKENLAANMLVRSGWKAAPTTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKATWHEI
LEEAKARASLGVKRCDVKFYGSDIDSRLVALAKRNAQNAGVFELIDFKVANALNVEPPAAEGYLITNPPYGERLGSVSEL
LQLYYQLGDKFKKEFGGWKVAMLCSDIELISALKLKADKQMKMFNGALECAFNLYTLHAQSTRRDTPVLPEGVDIADIAP
AFANRIKKNAKQLEKWAKKEGIDSYRLYDADIPEYNVAVDRYLDHIVVQEYMAPASIPEAVTKRRLSDVLLALPAAIGVD
PHKITMKTRERQKGTNQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLVGQKSKGRRVLNLFSYTGSAS
VHAALGGAKSVTTVDMSNTYLAWAKENFALNDLSGKQYEFVQADCLQWIRDSAHDKSAQYDLIFIDPPTFSNSKRMEDSF
DVQRDHVNLLGMLIKLLSPNGEIVFSNNKRKFKMDTDTLVKMKIKVENIDDLTLPMDYKRNPHIHNTWLITHA

Sequences:

>Translated_713_residues
MLNFFAAAPKGFEYSLAQELTEFGATEIKESVAGVYFTAPLALAYRITLWTRLASRIVLVIYKGPCESAEQLYNAAYCID
WSAHFSNRNTFSIDFHGTGGFINNTQFGALKIKDAIVDRFRDDGDARPNVARIDADIKIDAHFRNGVITIAMNFSGPSLH
QRGYRSTTGEAPLKENLAANMLVRSGWKAAPTTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKATWHEI
LEEAKARASLGVKRCDVKFYGSDIDSRLVALAKRNAQNAGVFELIDFKVANALNVEPPAAEGYLITNPPYGERLGSVSEL
LQLYYQLGDKFKKEFGGWKVAMLCSDIELISALKLKADKQMKMFNGALECAFNLYTLHAQSTRRDTPVLPEGVDIADIAP
AFANRIKKNAKQLEKWAKKEGIDSYRLYDADIPEYNVAVDRYLDHIVVQEYMAPASIPEAVTKRRLSDVLLALPAAIGVD
PHKITMKTRERQKGTNQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLVGQKSKGRRVLNLFSYTGSAS
VHAALGGAKSVTTVDMSNTYLAWAKENFALNDLSGKQYEFVQADCLQWIRDSAHDKSAQYDLIFIDPPTFSNSKRMEDSF
DVQRDHVNLLGMLIKLLSPNGEIVFSNNKRKFKMDTDTLVKMKIKVENIDDLTLPMDYKRNPHIHNTWLITHA
>Mature_713_residues
MLNFFAAAPKGFEYSLAQELTEFGATEIKESVAGVYFTAPLALAYRITLWTRLASRIVLVIYKGPCESAEQLYNAAYCID
WSAHFSNRNTFSIDFHGTGGFINNTQFGALKIKDAIVDRFRDDGDARPNVARIDADIKIDAHFRNGVITIAMNFSGPSLH
QRGYRSTTGEAPLKENLAANMLVRSGWKAAPTTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKATWHEI
LEEAKARASLGVKRCDVKFYGSDIDSRLVALAKRNAQNAGVFELIDFKVANALNVEPPAAEGYLITNPPYGERLGSVSEL
LQLYYQLGDKFKKEFGGWKVAMLCSDIELISALKLKADKQMKMFNGALECAFNLYTLHAQSTRRDTPVLPEGVDIADIAP
AFANRIKKNAKQLEKWAKKEGIDSYRLYDADIPEYNVAVDRYLDHIVVQEYMAPASIPEAVTKRRLSDVLLALPAAIGVD
PHKITMKTRERQKGTNQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLVGQKSKGRRVLNLFSYTGSAS
VHAALGGAKSVTTVDMSNTYLAWAKENFALNDLSGKQYEFVQADCLQWIRDSAHDKSAQYDLIFIDPPTFSNSKRMEDSF
DVQRDHVNLLGMLIKLLSPNGEIVFSNNKRKFKMDTDTLVKMKIKVENIDDLTLPMDYKRNPHIHNTWLITHA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=716, Percent_Identity=49.5810055865922, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=274, Percent_Identity=28.8321167883212, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_SHESA (A0KVL6)

Other databases:

- EMBL:   CP000469
- RefSeq:   YP_869241.1
- ProteinModelPortal:   A0KVL6
- SMR:   A0KVL6
- STRING:   A0KVL6
- GeneID:   4476559
- GenomeReviews:   CP000469_GR
- KEGG:   shn:Shewana3_1601
- NMPDR:   fig|94122.5.peg.1684
- eggNOG:   COG0116
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- PhylomeDB:   A0KVL6
- ProtClustDB:   PRK11783
- BioCyc:   SSP94122:SHEWANA3_1601-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 80103; Mature: 80103

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNFFAAAPKGFEYSLAQELTEFGATEIKESVAGVYFTAPLALAYRITLWTRLASRIVLV
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCEEEE
IYKGPCESAEQLYNAAYCIDWSAHFSNRNTFSIDFHGTGGFINNTQFGALKIKDAIVDRF
EECCCCHHHHHHCCEEEEEEEHHCCCCCCEEEEEECCCCCCCCCCCCCEEEHHHHHHHHH
RDDGDARPNVARIDADIKIDAHFRNGVITIAMNFSGPSLHQRGYRSTTGEAPLKENLAAN
CCCCCCCCCEEEEECEEEEEEEECCCEEEEEEECCCCCHHHCCCCCCCCCCCHHHHHHHH
MLVRSGWKAAPTTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKATWHEI
HHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHCCCHHHHHH
LEEAKARASLGVKRCDVKFYGSDIDSRLVALAKRNAQNAGVFELIDFKVANALNVEPPAA
HHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCCCCCCC
EGYLITNPPYGERLGSVSELLQLYYQLGDKFKKEFGGWKVAMLCSDIELISALKLKADKQ
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCHH
MKMFNGALECAFNLYTLHAQSTRRDTPVLPEGVDIADIAPAFANRIKKNAKQLEKWAKKE
HHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GIDSYRLYDADIPEYNVAVDRYLDHIVVQEYMAPASIPEAVTKRRLSDVLLALPAAIGVD
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PHKITMKTRERQKGTNQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLV
CCEEEEHHHHHHCCCHHHHHHHHHHHEEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHH
GQKSKGRRVLNLFSYTGSASVHAALGGAKSVTTVDMSNTYLAWAKENFALNDLSGKQYEF
CCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCEEEEECCCCEEECCCCCCCHH
VQADCLQWIRDSAHDKSAQYDLIFIDPPTFSNSKRMEDSFDVQRDHVNLLGMLIKLLSPN
HHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
GEIVFSNNKRKFKMDTDTLVKMKIKVENIDDLTLPMDYKRNPHIHNTWLITHA
CCEEECCCCEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MLNFFAAAPKGFEYSLAQELTEFGATEIKESVAGVYFTAPLALAYRITLWTRLASRIVLV
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCEEEE
IYKGPCESAEQLYNAAYCIDWSAHFSNRNTFSIDFHGTGGFINNTQFGALKIKDAIVDRF
EECCCCHHHHHHCCEEEEEEEHHCCCCCCEEEEEECCCCCCCCCCCCCEEEHHHHHHHHH
RDDGDARPNVARIDADIKIDAHFRNGVITIAMNFSGPSLHQRGYRSTTGEAPLKENLAAN
CCCCCCCCCEEEEECEEEEEEEECCCEEEEEEECCCCCHHHCCCCCCCCCCCHHHHHHHH
MLVRSGWKAAPTTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKATWHEI
HHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHCCCHHHHHH
LEEAKARASLGVKRCDVKFYGSDIDSRLVALAKRNAQNAGVFELIDFKVANALNVEPPAA
HHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCCCCCCC
EGYLITNPPYGERLGSVSELLQLYYQLGDKFKKEFGGWKVAMLCSDIELISALKLKADKQ
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCHH
MKMFNGALECAFNLYTLHAQSTRRDTPVLPEGVDIADIAPAFANRIKKNAKQLEKWAKKE
HHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GIDSYRLYDADIPEYNVAVDRYLDHIVVQEYMAPASIPEAVTKRRLSDVLLALPAAIGVD
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PHKITMKTRERQKGTNQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLV
CCEEEEHHHHHHCCCHHHHHHHHHHHEEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHH
GQKSKGRRVLNLFSYTGSASVHAALGGAKSVTTVDMSNTYLAWAKENFALNDLSGKQYEF
CCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCEEEEECCCCEEECCCCCCCHH
VQADCLQWIRDSAHDKSAQYDLIFIDPPTFSNSKRMEDSFDVQRDHVNLLGMLIKLLSPN
HHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
GEIVFSNNKRKFKMDTDTLVKMKIKVENIDDLTLPMDYKRNPHIHNTWLITHA
CCEEECCCCEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA