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The map label for this gene is ligA [H]

Identifier: 117920017

GI number: 117920017

Start: 1835787

End: 1837862

Strand: Direct

Name: ligA [H]

Synonym: Shewana3_1569

Alternate gene names: 117920017

Gene position: 1835787-1837862 (Clockwise)

Preceding gene: 117920016

Following gene: 117920018

Centisome position: 36.92

GC content: 52.41

Gene sequence:

>2076_bases
ATGAATATGCAAGACATTCAACTCGATAAACGCCTATCTGAACTGCTTTCTCAAGCTGTCACGCCACACAATGCGCAGCC
AATAATGCAGGCGCTCTGCCAATCTCTGAATGAACACAATATTCGTTACTATGTCGATGATGCGCCAAGCATTACCGACT
CCGAATACGACAGATTAATGCAGCGTCTGAAACAGCTTGAGGCCGAGTATCCTCAGTTTGTTGCTGCCGACTCGCCCACC
CAAAGAGTGGGCGGTATGGCACTGGCCAAGTTTGAGCAAATCACTCACCTTAAACCTATGTTAAGTCTCGATAATGCCTT
CGATGAGGCAGACTTTAGCGCGTTTCATAAACGTGTCAGCGACAGAGTTGGCGAAGTGAGTTTTTGTTGTGAGCCGAAAC
TCGATGGTTTGGCGGTGAGTATTTTGTATCGCAATGGTGTGCTTGAGCGCGCTGCGACTCGGGGTGATGGTACCGTGGGC
GAAGATATTACCGAAAACGTTAAAACCATTAAGTCGATTCCGCTCAAACTGCGCGGTGACAACTATCCCGAATTGGTCGA
AGTGCGCGGCGAAGCCTTTATGCCCAAGGCGGCGTTTGAGGCGCTAAATGAGCGTGCACGCTTAAAGGATGAAAAACTGT
TCGTCAATCCGCGTAATGCGGCGGCGGGCAGCTTACGTCAGCTCGACAGTAAAATTACCGCGTCACGGGCGCTGTCTTTT
TATGCCTATGCCTTAGGCGTGGTGGAGCCGACTTCCCATGAGCTAGCTAAGACGCACTACGAGCAATTGCAGCAACTAAA
ATCTTGGGGGCTGCCAGTCAGCAGCGAGATCAAAGTCTGCGACGAACTCAGCCAAGTCTTTGCCTACTATCAAGATATAT
TGACTCGCCGCGCCGACTTACCCTTTGAGATCGATGGCGTGGTGATGAAGGTGAACGATATCGCCCAGCAGCAAACTTTA
GGGTTTGTGGCCAAATCTCCCCGTTGGGCGATTGCCTATAAGTTCCCCGCACAGGAAGAAATGACCCTGCTCGAAGGCGT
AGATTTTCAGGTCGGCCGCACTGGCGCAGTGACTCCAGTCGCGCGCTTAAAACCTGTGTTTGTCGGCGGCGTGACGGTAT
CGAACGCCACCTTACACAATGCCGATGAAATCGCTCGTTTAGGTGTAATGGTGGGTGATACTGTGATTATCCGCCGCGCC
GGTGATGTGATCCCGCAAATTGTCGCCATAGTGCCCGAGCGTCGCCCAGAAGATGCTAAGGCGATTGCCTTCCCGCAACA
TTGCCCCGTTTGCGGCAGTTTGGTTGAGCGATTAGAAGGTGAAGCCGTGGCCCGTTGTAGCGGCGGCTTATTCTGTGAGG
CGCAGCGTAAAGAGGCGATCAAGCATTTTGCCTCCCGCAAGGCATTAGATATCGACGGCATGGGCGATAAAATTGTCGAG
CAGTTGATTGATAAGGAGCTGGTTCAAAGCCCGGCGGACTTATTTAAACTCACCGCCTCGATGATGACCATGCTCGACCG
TATGGGAATTAAGTCGGCCACCAATCTTGCGCTGGCGATTGAAGCGGCTAAAACCACCACCTTGCCACGTTTCCTTTATG
CCTTAGGCATTCGAGAGGTGGGGGAGACCACGGCGGCGAACCTGGCGACTCACTTTGGCAGCCTAGAGGCGCTGCGTGTA
GCTACTATTGAGCAACTTATTCAAGTTGAAGATATTGGTGAAGTGGTTGCCCAGCATGTGGCGCATTTCTTTGCCCAGCC
CCATAACCTAGAGGTGATCGATGCCTTGATTGCGGCTGGCGTCAACTGGCCTGCGATTGCAGCACCAAGCGCCGATGAGC
AGCCGTTGAAGGGGCAGACTTGGGTGCTAACCGGCACCTTAAATCAACTCAACCGTAACGATGCTAAGGCGCAACTACAG
GCCTTAGGCGCCAAAGTGGCGGGCAGTGTATCGAAAAATACCGATTGCTTAGTTGCGGGTGAAGCGGCGGGTTCTAAGCT
TGCCAAAGCGCAGGAGCTTGGCGTGAAAGTGATTGGTGAAGATGAGTTGTTGGCTCTTCTAGCAGCAAATAGCTAA

Upstream 100 bases:

>100_bases
ACGCTTAATGCAACGCCGCGAGACCGCGGTGATAGACAGATGGGGCCGCTAAATGCGGCCTCATTTTTTGCAACTTTTTG
ATGCTTAAGAATTCTGGATA

Downstream 100 bases:

>100_bases
CACGCTGCCGCTTCATTGATAAAGCCCTGCCTTGCGGGGCTTTTTTATGGCTAAGATATGGGGCCGTGGTTTGTGTGGTG
AACTTGGCATAACACAACTT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 691; Mature: 691

Protein sequence:

>691_residues
MNMQDIQLDKRLSELLSQAVTPHNAQPIMQALCQSLNEHNIRYYVDDAPSITDSEYDRLMQRLKQLEAEYPQFVAADSPT
QRVGGMALAKFEQITHLKPMLSLDNAFDEADFSAFHKRVSDRVGEVSFCCEPKLDGLAVSILYRNGVLERAATRGDGTVG
EDITENVKTIKSIPLKLRGDNYPELVEVRGEAFMPKAAFEALNERARLKDEKLFVNPRNAAAGSLRQLDSKITASRALSF
YAYALGVVEPTSHELAKTHYEQLQQLKSWGLPVSSEIKVCDELSQVFAYYQDILTRRADLPFEIDGVVMKVNDIAQQQTL
GFVAKSPRWAIAYKFPAQEEMTLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNADEIARLGVMVGDTVIIRRA
GDVIPQIVAIVPERRPEDAKAIAFPQHCPVCGSLVERLEGEAVARCSGGLFCEAQRKEAIKHFASRKALDIDGMGDKIVE
QLIDKELVQSPADLFKLTASMMTMLDRMGIKSATNLALAIEAAKTTTLPRFLYALGIREVGETTAANLATHFGSLEALRV
ATIEQLIQVEDIGEVVAQHVAHFFAQPHNLEVIDALIAAGVNWPAIAAPSADEQPLKGQTWVLTGTLNQLNRNDAKAQLQ
ALGAKVAGSVSKNTDCLVAGEAAGSKLAKAQELGVKVIGEDELLALLAANS

Sequences:

>Translated_691_residues
MNMQDIQLDKRLSELLSQAVTPHNAQPIMQALCQSLNEHNIRYYVDDAPSITDSEYDRLMQRLKQLEAEYPQFVAADSPT
QRVGGMALAKFEQITHLKPMLSLDNAFDEADFSAFHKRVSDRVGEVSFCCEPKLDGLAVSILYRNGVLERAATRGDGTVG
EDITENVKTIKSIPLKLRGDNYPELVEVRGEAFMPKAAFEALNERARLKDEKLFVNPRNAAAGSLRQLDSKITASRALSF
YAYALGVVEPTSHELAKTHYEQLQQLKSWGLPVSSEIKVCDELSQVFAYYQDILTRRADLPFEIDGVVMKVNDIAQQQTL
GFVAKSPRWAIAYKFPAQEEMTLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNADEIARLGVMVGDTVIIRRA
GDVIPQIVAIVPERRPEDAKAIAFPQHCPVCGSLVERLEGEAVARCSGGLFCEAQRKEAIKHFASRKALDIDGMGDKIVE
QLIDKELVQSPADLFKLTASMMTMLDRMGIKSATNLALAIEAAKTTTLPRFLYALGIREVGETTAANLATHFGSLEALRV
ATIEQLIQVEDIGEVVAQHVAHFFAQPHNLEVIDALIAAGVNWPAIAAPSADEQPLKGQTWVLTGTLNQLNRNDAKAQLQ
ALGAKVAGSVSKNTDCLVAGEAAGSKLAKAQELGVKVIGEDELLALLAANS
>Mature_691_residues
MNMQDIQLDKRLSELLSQAVTPHNAQPIMQALCQSLNEHNIRYYVDDAPSITDSEYDRLMQRLKQLEAEYPQFVAADSPT
QRVGGMALAKFEQITHLKPMLSLDNAFDEADFSAFHKRVSDRVGEVSFCCEPKLDGLAVSILYRNGVLERAATRGDGTVG
EDITENVKTIKSIPLKLRGDNYPELVEVRGEAFMPKAAFEALNERARLKDEKLFVNPRNAAAGSLRQLDSKITASRALSF
YAYALGVVEPTSHELAKTHYEQLQQLKSWGLPVSSEIKVCDELSQVFAYYQDILTRRADLPFEIDGVVMKVNDIAQQQTL
GFVAKSPRWAIAYKFPAQEEMTLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNADEIARLGVMVGDTVIIRRA
GDVIPQIVAIVPERRPEDAKAIAFPQHCPVCGSLVERLEGEAVARCSGGLFCEAQRKEAIKHFASRKALDIDGMGDKIVE
QLIDKELVQSPADLFKLTASMMTMLDRMGIKSATNLALAIEAAKTTTLPRFLYALGIREVGETTAANLATHFGSLEALRV
ATIEQLIQVEDIGEVVAQHVAHFFAQPHNLEVIDALIAAGVNWPAIAAPSADEQPLKGQTWVLTGTLNQLNRNDAKAQLQ
ALGAKVAGSVSKNTDCLVAGEAAGSKLAKAQELGVKVIGEDELLALLAANS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=666, Percent_Identity=62.012012012012, Blast_Score=847, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=522, Percent_Identity=22.0306513409962, Blast_Score=112, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75366; Mature: 75366

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMQDIQLDKRLSELLSQAVTPHNAQPIMQALCQSLNEHNIRYYVDDAPSITDSEYDRLM
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHH
QRLKQLEAEYPQFVAADSPTQRVGGMALAKFEQITHLKPMLSLDNAFDEADFSAFHKRVS
HHHHHHHHHCCCEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
DRVGEVSFCCEPKLDGLAVSILYRNGVLERAATRGDGTVGEDITENVKTIKSIPLKLRGD
HHCCCEEEEECCCCCCEEEEEEHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCEEECCC
NYPELVEVRGEAFMPKAAFEALNERARLKDEKLFVNPRNAAAGSLRQLDSKITASRALSF
CCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
YAYALGVVEPTSHELAKTHYEQLQQLKSWGLPVSSEIKVCDELSQVFAYYQDILTRRADL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PFEIDGVVMKVNDIAQQQTLGFVAKSPRWAIAYKFPAQEEMTLLEGVDFQVGRTGAVTPV
CEEECCEEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCHH
ARLKPVFVGGVTVSNATLHNADEIARLGVMVGDTVIIRRAGDVIPQIVAIVPERRPEDAK
HHCCEEEECCEEECCCCCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCCCCCCCCCC
AIAFPQHCPVCGSLVERLEGEAVARCSGGLFCEAQRKEAIKHFASRKALDIDGMGDKIVE
EEECCCCCCHHHHHHHHHCCCCEECCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHH
QLIDKELVQSPADLFKLTASMMTMLDRMGIKSATNLALAIEAAKTTTLPRFLYALGIREV
HHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHH
GETTAANLATHFGSLEALRVATIEQLIQVEDIGEVVAQHVAHFFAQPHNLEVIDALIAAG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
VNWPAIAAPSADEQPLKGQTWVLTGTLNQLNRNDAKAQLQALGAKVAGSVSKNTDCLVAG
CCCCCEECCCCCCCCCCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEC
EAAGSKLAKAQELGVKVIGEDELLALLAANS
CCCCHHHHHHHHCCEEEECCCCEEEEEECCC
>Mature Secondary Structure
MNMQDIQLDKRLSELLSQAVTPHNAQPIMQALCQSLNEHNIRYYVDDAPSITDSEYDRLM
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHH
QRLKQLEAEYPQFVAADSPTQRVGGMALAKFEQITHLKPMLSLDNAFDEADFSAFHKRVS
HHHHHHHHHCCCEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
DRVGEVSFCCEPKLDGLAVSILYRNGVLERAATRGDGTVGEDITENVKTIKSIPLKLRGD
HHCCCEEEEECCCCCCEEEEEEHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCEEECCC
NYPELVEVRGEAFMPKAAFEALNERARLKDEKLFVNPRNAAAGSLRQLDSKITASRALSF
CCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
YAYALGVVEPTSHELAKTHYEQLQQLKSWGLPVSSEIKVCDELSQVFAYYQDILTRRADL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PFEIDGVVMKVNDIAQQQTLGFVAKSPRWAIAYKFPAQEEMTLLEGVDFQVGRTGAVTPV
CEEECCEEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCHH
ARLKPVFVGGVTVSNATLHNADEIARLGVMVGDTVIIRRAGDVIPQIVAIVPERRPEDAK
HHCCEEEECCEEECCCCCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCCCCCCCCCC
AIAFPQHCPVCGSLVERLEGEAVARCSGGLFCEAQRKEAIKHFASRKALDIDGMGDKIVE
EEECCCCCCHHHHHHHHHCCCCEECCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHH
QLIDKELVQSPADLFKLTASMMTMLDRMGIKSATNLALAIEAAKTTTLPRFLYALGIREV
HHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHH
GETTAANLATHFGSLEALRVATIEQLIQVEDIGEVVAQHVAHFFAQPHNLEVIDALIAAG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
VNWPAIAAPSADEQPLKGQTWVLTGTLNQLNRNDAKAQLQALGAKVAGSVSKNTDCLVAG
CCCCCEECCCCCCCCCCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEC
EAAGSKLAKAQELGVKVIGEDELLALLAANS
CCCCHHHHHHHHCCEEEECCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]