Click here to switch to the map view.

The map label for this gene is purA2 [H]

Identifier: 117919521

GI number: 117919521

Start: 1257627

End: 1258883

Strand: Reverse

Name: purA2 [H]

Synonym: Shewana3_1072

Alternate gene names: 117919521

Gene position: 1258883-1257627 (Counterclockwise)

Preceding gene: 117919523

Following gene: 117919520

Centisome position: 25.32

GC content: 49.72

Gene sequence:

>1257_bases
ATGCCGTCTATCGTAGTTGTTGGTGCAAATTGGGGTGATGAAGGCAAAGGCCGAATCGTAGATTATTTAGCCGCAGATGC
TGCAGCGAGTGTCCGCTTCCAAGGCGGTAACAACGCAGGCCATACGGTCGTTAACGACTTTGGCACCTTTAAATTACACC
AATTACCTAGCGGTATTTTCAATCCAAACTGCGTTGCAGTACTTGGCCCTGGCATGGTGATCAGCCCAGAAAAACTCAGT
GTTGAAATCGCCGAAGTCGAAGCGGCTGGCATTGAAGTTAAACTGTGCATTTCTGACCGTGCGACCCTGTGCTTACCTAT
CCATGCACTGGAAGATACCTTAGAAGAACAACGTTTAGGCGATCTGGCCTATGGTTCAACCCGTCAAGGGATTGCCCCAG
CCTATGGCGACCGCGTAATGAAGAAAGGTATTCTGGTTGGCTGGTTAAACCAACCCGAAGTGCTGCAAGAACGCATTCAA
TTTATGCTCGACTGGAAACTGCCCCAGTTAAAAGCATTGTACCCAAGCTGTGACTTTAGCCAAACGGCCGCCGAAATGAC
CCAATGGTTGTTGGATGTGACCGCCCCATGGCGTCCATTTATTTGCAACGTGACAGAGCCATTAAAAGCCTTACAAAAAC
AAGATGCTAACTTGTTATTTGAAGCGCAATTAGGCGCGGGTCGTGACTTGATTTACGGTGAGTATCCTTACACTACCTCT
TCTAACGTGACGGCGGCTTTCGCTGGTCTGGGTAGTGGTTTACCGGCTCTGCGCCCAGAGCGTGTGATTGCTGTAGCGAA
AGCCTTCAGCTCATCTGTCGGTACTGGCACCTTAGTGACTGCGATGGAAGAGCAGGATGATTTCCGTAAAAACGCCAACG
AGTTTGGTGCAACTACGGGTCGTCCACGTGATATGGGTTACTTCGATGCGGTAGCAACTCGTAACGGTGTTGAACTGCAA
GCTGCGACTGAAATCGCCCTGACCAAGATTGACTGCCTGACTGGCATGAAAGATCTTAAGATTTGTGTTGCCTACGAAGG
CGAGCACACTGAAAACCCCATTTGGCCTCAAACTGCGGCATTAAGCCCAGTGTATGAACAAATGGAAAGCTGGAGCGAAG
ACATCACTGGCTGTCGCACCTTCGAAAGCCTACCAAAGGCAGCACAACATTATGTTGAGCGCATCGAAGCCTTAATGGGT
GTGCGTGTGAGCATGGTGTCTGTAGGACCTGAACGCGACCAGATGATCATTCGCTAA

Upstream 100 bases:

>100_bases
ATGATATATGATTTCCTTATCACGACGATAACTATAATTAATTTCTCTTATTCATTAAGCAGGTCTAGCATAATCGCTTA
ATTCCTGTGGAGATGATGTT

Downstream 100 bases:

>100_bases
GCGCTAGGCTTTAGCCACCGATTACCCTACAAAAAAGCCATATTCGTTTCGGCGAATATGGCTTTTTACTTTGTGTCGTC
TAGATATTCTCAATCCTAAC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase 2; AdSS 2; IMP--aspartate ligase 2 [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MPSIVVVGANWGDEGKGRIVDYLAADAAASVRFQGGNNAGHTVVNDFGTFKLHQLPSGIFNPNCVAVLGPGMVISPEKLS
VEIAEVEAAGIEVKLCISDRATLCLPIHALEDTLEEQRLGDLAYGSTRQGIAPAYGDRVMKKGILVGWLNQPEVLQERIQ
FMLDWKLPQLKALYPSCDFSQTAAEMTQWLLDVTAPWRPFICNVTEPLKALQKQDANLLFEAQLGAGRDLIYGEYPYTTS
SNVTAAFAGLGSGLPALRPERVIAVAKAFSSSVGTGTLVTAMEEQDDFRKNANEFGATTGRPRDMGYFDAVATRNGVELQ
AATEIALTKIDCLTGMKDLKICVAYEGEHTENPIWPQTAALSPVYEQMESWSEDITGCRTFESLPKAAQHYVERIEALMG
VRVSMVSVGPERDQMIIR

Sequences:

>Translated_418_residues
MPSIVVVGANWGDEGKGRIVDYLAADAAASVRFQGGNNAGHTVVNDFGTFKLHQLPSGIFNPNCVAVLGPGMVISPEKLS
VEIAEVEAAGIEVKLCISDRATLCLPIHALEDTLEEQRLGDLAYGSTRQGIAPAYGDRVMKKGILVGWLNQPEVLQERIQ
FMLDWKLPQLKALYPSCDFSQTAAEMTQWLLDVTAPWRPFICNVTEPLKALQKQDANLLFEAQLGAGRDLIYGEYPYTTS
SNVTAAFAGLGSGLPALRPERVIAVAKAFSSSVGTGTLVTAMEEQDDFRKNANEFGATTGRPRDMGYFDAVATRNGVELQ
AATEIALTKIDCLTGMKDLKICVAYEGEHTENPIWPQTAALSPVYEQMESWSEDITGCRTFESLPKAAQHYVERIEALMG
VRVSMVSVGPERDQMIIR
>Mature_417_residues
PSIVVVGANWGDEGKGRIVDYLAADAAASVRFQGGNNAGHTVVNDFGTFKLHQLPSGIFNPNCVAVLGPGMVISPEKLSV
EIAEVEAAGIEVKLCISDRATLCLPIHALEDTLEEQRLGDLAYGSTRQGIAPAYGDRVMKKGILVGWLNQPEVLQERIQF
MLDWKLPQLKALYPSCDFSQTAAEMTQWLLDVTAPWRPFICNVTEPLKALQKQDANLLFEAQLGAGRDLIYGEYPYTTSS
NVTAAFAGLGSGLPALRPERVIAVAKAFSSSVGTGTLVTAMEEQDDFRKNANEFGATTGRPRDMGYFDAVATRNGVELQA
ATEIALTKIDCLTGMKDLKICVAYEGEHTENPIWPQTAALSPVYEQMESWSEDITGCRTFESLPKAAQHYVERIEALMGV
RVSMVSVGPERDQMIIR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=436, Percent_Identity=34.6330275229358, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI22748717, Length=427, Percent_Identity=34.4262295081967, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=32.1608040201005, Blast_Score=176, Evalue=4e-44,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=38.5514018691589, Blast_Score=278, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI25146555, Length=432, Percent_Identity=33.1018518518519, Blast_Score=220, Evalue=9e-58,
Organism=Caenorhabditis elegans, GI25146553, Length=432, Percent_Identity=33.1018518518519, Blast_Score=220, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=30.8584686774942, Blast_Score=210, Evalue=4e-55,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=35.0588235294118, Blast_Score=222, Evalue=5e-58,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 45538; Mature: 45407

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIVVVGANWGDEGKGRIVDYLAADAAASVRFQGGNNAGHTVVNDFGTFKLHQLPSGIF
CCCEEEEECCCCCCCCCCEEHHHHHCCCEEEEEECCCCCCCCEECCCCCEEEECCCCCCC
NPNCVAVLGPGMVISPEKLSVEIAEVEAAGIEVKLCISDRATLCLPIHALEDTLEEQRLG
CCCEEEEECCCEEECCHHHEEEEEEEEECCEEEEEEECCCCEEEEEHHHHHHHHHHHHCC
DLAYGSTRQGIAPAYGDRVMKKGILVGWLNQPEVLQERIQFMLDWKLPQLKALYPSCDFS
CCCCCCCCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCH
QTAAEMTQWLLDVTAPWRPFICNVTEPLKALQKQDANLLFEAQLGAGRDLIYGEYPYTTS
HHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCC
SNVTAAFAGLGSGLPALRPERVIAVAKAFSSSVGTGTLVTAMEEQDDFRKNANEFGATTG
CCCEEEEHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCHHHHCCCCC
RPRDMGYFDAVATRNGVELQAATEIALTKIDCLTGMKDLKICVAYEGEHTENPIWPQTAA
CCCCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHH
LSPVYEQMESWSEDITGCRTFESLPKAAQHYVERIEALMGVRVSMVSVGPERDQMIIR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEECC
>Mature Secondary Structure 
PSIVVVGANWGDEGKGRIVDYLAADAAASVRFQGGNNAGHTVVNDFGTFKLHQLPSGIF
CCEEEEECCCCCCCCCCEEHHHHHCCCEEEEEECCCCCCCCEECCCCCEEEECCCCCCC
NPNCVAVLGPGMVISPEKLSVEIAEVEAAGIEVKLCISDRATLCLPIHALEDTLEEQRLG
CCCEEEEECCCEEECCHHHEEEEEEEEECCEEEEEEECCCCEEEEEHHHHHHHHHHHHCC
DLAYGSTRQGIAPAYGDRVMKKGILVGWLNQPEVLQERIQFMLDWKLPQLKALYPSCDFS
CCCCCCCCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCH
QTAAEMTQWLLDVTAPWRPFICNVTEPLKALQKQDANLLFEAQLGAGRDLIYGEYPYTTS
HHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCC
SNVTAAFAGLGSGLPALRPERVIAVAKAFSSSVGTGTLVTAMEEQDDFRKNANEFGATTG
CCCEEEEHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCHHHHCCCCC
RPRDMGYFDAVATRNGVELQAATEIALTKIDCLTGMKDLKICVAYEGEHTENPIWPQTAA
CCCCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHH
LSPVYEQMESWSEDITGCRTFESLPKAAQHYVERIEALMGVRVSMVSVGPERDQMIIR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA