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The map label for this gene is prfC [H]

Identifier: 117919486

GI number: 117919486

Start: 1212358

End: 1213938

Strand: Direct

Name: prfC [H]

Synonym: Shewana3_1037

Alternate gene names: 117919486

Gene position: 1212358-1213938 (Clockwise)

Preceding gene: 117919485

Following gene: 117919487

Centisome position: 24.38

GC content: 47.94

Gene sequence:

>1581_bases
ATGTCAGACAATAAAGTAGAGGTCGATAAACGTCGCACCTTCGCGATTATATCGCACCCCGACGCGGGTAAAACCACCAT
TACCGAAAAAGTGCTGTTATTCGGAAACGCGCTGCAGAAAGCGGGTACCGTTAAGGGCAAAAAATCGGGTCAGCATGCTA
AGTCTGACTGGATGGAAATGGAAAAAGACCGTGGTATTTCGATTACTACGTCTGTCATGCAGTTCCCCTATGGTGGCGCG
CTCGTTAACCTACTCGATACTCCCGGCCACGAAGACTTCTCAGAAGATACTTACCGCACCTTGACGGCGGTTGACTCTTG
CTTGATGGTTATCGACTCGGCAAAAGGGGTAGAGGATCGTACCATCAAATTGATGGAAGTCACGCGTCTGCGCGATACGC
CTATCGTCACCTTTATGAACAAACTCGACCGTGATATTCGCGATCCCATCGAACTGATGGATGAGGTTGAAGATGTTCTT
AATATCGCCTGTGCGCCTATCACTTGGCCTATTGGCAGCGGTAAAGAGTTCAAAGGTGTTTACCATATTCTGCGTGATGA
AGTGGTACTGTATCAGAGCGGTATGGGACACACGATTCAAGAGCGCCGCGTGATTGAAGGCATCGATAACCCTGAGCTGG
ATAAGGCGATTGGTAGTTATGCTGCCGATTTACGTGATGAAATGGAGTTGGTTCGCGGTGCATCGAATGAATTTGATCAT
CAAGCCTTCTTAAAGGGTGAACTTACGCCAGTCTTCTTCGGTACTGCATTGGGTAACTTCGGTGTTGACCATATCCTCGA
TGGTATTGTGGAGTGGGCGCCAAAGCCGTTGCCTCGTGAAAGCGATGCGCGGATGATCATGCCTGACGAAGAAAAATTCA
CCGGTTTTGTGTTTAAAATCCAAGCTAACATGGATCCTAAACACCGTGACCGCGTTGCCTTTATGCGCGTTTGCTCGGGT
CGCTATGAGCAAGGCATGAAGATGCACCATGTGCGCATTGGCAAAGATGTTAACGTCAGTGATGCCTTGACCTTTATGGC
GGGTGACCGAGAACGTGCTGAAGTGGCGTATCCTGGTGATATTATTGGTTTACATAACCATGGGACCATTCGTATTGGTG
ATACTTTCACCCAAGGTGAAAAGTTCCGCTTTACCGGTGTACCTAACTTCGCACCTGAAATGTTCCGCCGTATTCGTCTG
CGTGATCCACTCAAGCAAAAACAATTGCTTAAGGGCCTAGTTCAGTTGTCTGAAGAAGGTGCTGTGCAGGTATTCCGTCC
ATTAGATTCGAACGATTTGATCGTTGGGGCTGTGGGTGTGCTGCAGTTTGAAGTGGTTGTCGGCCGCTTAAAGAGCGAGT
ACAACGTTGAAGCGATTTACGAAGGTATTAGCGTTTCAACGGCACGCTGGGTTTATTGTAAAGATGAGCGTAAGCTAGAA
GAGTTCCGCCGTAAGTGCAGCCAAAACTTGGCGCTCGATGGTGGCGATAACTTAACTTATATTGCGCCAACTATGGTTAA
CTTAAATCTTTCGATGGAAAGATACCCTGATATCGAGTTCGCTAAAACGCGCGAACACTAA

Upstream 100 bases:

>100_bases
AGCCATATCGCCCGAGACACATCTTGGGCTTTACCTAGCCAAGTACGTACTGAGGCGCTATAATTTGCGCCTTTTTTAGC
GATACGAGTGCATAGGTTAA

Downstream 100 bases:

>100_bases
CAGGTAAGTAACCTAGGCTAAAAGGCGGTGATTCACCGCCTTTTTTGTATCTACAATTTGTGGGATGACTCATGATTCAT
TGGATAACTCGGGCGTGGCG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSDNKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYGGA
LVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEDRTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIELMDEVEDVL
NIACAPITWPIGSGKEFKGVYHILRDEVVLYQSGMGHTIQERRVIEGIDNPELDKAIGSYAADLRDEMELVRGASNEFDH
QAFLKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDARMIMPDEEKFTGFVFKIQANMDPKHRDRVAFMRVCSG
RYEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEVAYPGDIIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRL
RDPLKQKQLLKGLVQLSEEGAVQVFRPLDSNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERKLE
EFRRKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH

Sequences:

>Translated_526_residues
MSDNKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYGGA
LVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEDRTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIELMDEVEDVL
NIACAPITWPIGSGKEFKGVYHILRDEVVLYQSGMGHTIQERRVIEGIDNPELDKAIGSYAADLRDEMELVRGASNEFDH
QAFLKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDARMIMPDEEKFTGFVFKIQANMDPKHRDRVAFMRVCSG
RYEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEVAYPGDIIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRL
RDPLKQKQLLKGLVQLSEEGAVQVFRPLDSNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERKLE
EFRRKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH
>Mature_525_residues
SDNKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYGGAL
VNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEDRTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIELMDEVEDVLN
IACAPITWPIGSGKEFKGVYHILRDEVVLYQSGMGHTIQERRVIEGIDNPELDKAIGSYAADLRDEMELVRGASNEFDHQ
AFLKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDARMIMPDEEKFTGFVFKIQANMDPKHRDRVAFMRVCSGR
YEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEVAYPGDIIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRLR
DPLKQKQLLKGLVQLSEEGAVQVFRPLDSNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERKLEE
FRRKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=481, Percent_Identity=27.027027027027, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI19923640, Length=510, Percent_Identity=26.2745098039216, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=27.9187817258883, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI25306287, Length=482, Percent_Identity=25.7261410788382, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI157426893, Length=180, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI94966754, Length=157, Percent_Identity=29.2993630573248, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=28.8590604026846, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=77.9270633397313, Blast_Score=866, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=498, Percent_Identity=25.1004016064257, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=30.7692307692308, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI48994988, Length=198, Percent_Identity=29.2929292929293, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=488, Percent_Identity=26.6393442622951, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17556745, Length=183, Percent_Identity=30.6010928961749, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=31.8518518518519, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17506493, Length=163, Percent_Identity=29.4478527607362, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=480, Percent_Identity=27.0833333333333, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322359, Length=425, Percent_Identity=25.1764705882353, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324707, Length=170, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320593, Length=170, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=174, Percent_Identity=21.8390804597701, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24582462, Length=475, Percent_Identity=26.1052631578947, Blast_Score=159, Evalue=6e-39,
Organism=Drosophila melanogaster, GI221458488, Length=392, Percent_Identity=25.2551020408163, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI78706572, Length=235, Percent_Identity=29.3617021276596, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585709, Length=169, Percent_Identity=30.1775147928994, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24585711, Length=169, Percent_Identity=30.1775147928994, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24585713, Length=169, Percent_Identity=30.1775147928994, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59375; Mature: 59243

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEM
CCCCCEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EKDRGISITTSVMQFPYGGALVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEDR
HHCCCCEEEHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
TIKLMEVTRLRDTPIVTFMNKLDRDIRDPIELMDEVEDVLNIACAPITWPIGSGKEFKGV
EEEEEEHHHCCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHEECCEEECCCCCCHHHHH
YHILRDEVVLYQSGMGHTIQERRVIEGIDNPELDKAIGSYAADLRDEMELVRGASNEFDH
HHHHHHCEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QAFLKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDARMIMPDEEKFTGFVFKI
HHHHCCCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEEE
QANMDPKHRDRVAFMRVCSGRYEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEVAYPGD
EECCCCCHHHHHHHHHHHCCHHHCCCEEEEEEECCCCCHHHHHEEEECCCCCCEEECCCC
IIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRLRDPLKQKQLLKGLVQLSEEG
EEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
AVQVFRPLDSNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERKLE
CEEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHCCCEECEEEEEEECCHHHHH
EFRRKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH
HHHHHHHCCCEECCCCCEEEEEEEEEEEEECHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SDNKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEM
CCCCEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EKDRGISITTSVMQFPYGGALVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEDR
HHCCCCEEEHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
TIKLMEVTRLRDTPIVTFMNKLDRDIRDPIELMDEVEDVLNIACAPITWPIGSGKEFKGV
EEEEEEHHHCCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHEECCEEECCCCCCHHHHH
YHILRDEVVLYQSGMGHTIQERRVIEGIDNPELDKAIGSYAADLRDEMELVRGASNEFDH
HHHHHHCEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QAFLKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDARMIMPDEEKFTGFVFKI
HHHHCCCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEEE
QANMDPKHRDRVAFMRVCSGRYEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEVAYPGD
EECCCCCHHHHHHHHHHHCCHHHCCCEEEEEEECCCCCHHHHHEEEECCCCCCEEECCCC
IIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRLRDPLKQKQLLKGLVQLSEEG
EEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
AVQVFRPLDSNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERKLE
CEEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHCCCEECEEEEEEECCHHHHH
EFRRKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH
HHHHHHHCCCEECCCCCEEEEEEEEEEEEECHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA