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The map label for this gene is miaB [H]

Identifier: 117919457

GI number: 117919457

Start: 1178510

End: 1179934

Strand: Reverse

Name: miaB [H]

Synonym: Shewana3_1008

Alternate gene names: 117919457

Gene position: 1179934-1178510 (Counterclockwise)

Preceding gene: 117919458

Following gene: 117919456

Centisome position: 23.73

GC content: 49.26

Gene sequence:

>1425_bases
ATGAGCAAAAAACTCCATATTAAAACTTGGGGCTGTCAAATGAATGAGTATGACTCATCCAAGATGGCGGATCTTTTAGG
CGAATACCAAGGTTACACCTTGACTGAAGAAGCAGAAGAAGCGGACATTCTACTGCTGAACACTTGTTCTATCCGCGAAA
AAGCGCAGGAAAAAGTGTTCCATCAGTTAGGTCGTTGGAAAACCCTTAAAGATAAAAATCCCGATCTGATCATTGGTGTG
GGTGGTTGTGTTGCATCGCAGGAAGGTAAAGCGATCAAAGACCGTGCTCAGTGCGTCGACATCATCTTTGGACCACAGAC
ACTACACCGTCTGCCAGATATGATCGACCAAGTACGCCGTGGCGAAAAGGCCGTGATCGACGTTAGCTTCCCTGAAATCG
AGAAATTTGACCGTCTACCAGAACCGCGCGCCGAAGGCCCAACGGCGTTTGTGTCTATCATGGAAGGTTGCAGCAAGTAC
TGTTCATTCTGCGTGGTGCCTTATACCCGTGGTGAAGAAGTCAGCCGTCCATCGGACGATATCATCCTCGAGATTGCCCA
ACTGGCAGAACAAGGTGTGCGCGAAGTCAACCTGCTCGGTCAAAACGTTAACGCCTACCGTGGTGCAACCCACGACGGCG
GCATTTGTACCTTTGCCGAGCTATTACGCTATGTTGCCGCTATCGATGGTATCGACCGTATTCGCTTCACCACCAGTCAC
CCGATTGAATTTACGCAGGACATTATCGACGTTTACGAAGATACTCCTGAATTAGTCAGCTTCCTGCACTTACCAGTGCA
ATCGGGTTCTGACCGCATTCTGACGGCAATGAAGCGTGGCCATATGGCGATAGAGTACAAGTCAATCATCCGCCGTCTGC
GCAAAGCCCGTCCTGATATCCAAATCAGCTCTGACTTTATCATTGGCTTCCCTGGCGAAACCAAAGAAGACTTCGCCGAT
ACCATGAAGCTGATTGAAGATGTGGCGTTTGACCACAGCTTTAGCTTTATCTACAGTGCGCGCCCTGGTACACCTGCGGC
AGATTTACCCGATGACGTTGATATGGAAGAGAAAAAACAACGTCTGGCGATTCTGCAGGACCGTATCACTCAACAAGCCA
TGCGTTACAGCCGCCATATGATGGGCACAGTACAACGTATTCTGGTTGAAGGCCCATCGGTGAAAAACCCTATGGAGCTG
CGCGGCCGTACCGAAAACAACCGTGTCGTCAACTTCGAAGGTCAGCCAAAGCACATCGGTAGCTTTGTGGATGTCGAAAT
TGTTGACGTTTACACTAACTCACTGCGCGGTAAATTTATCCGTGGTGAAGATGAAATGGATCTGCGCCGCAACCTGCGCC
CATCGGATATTCTGGCCAAACACAAACAGGATGACGATCTCGGTGTGACACAATTTAAACCTTAA

Upstream 100 bases:

>100_bases
AAAATGTCGCGCTATTTTTACTGGGCGGCCGGATTTGTCTGTAACAGGGCAAAGGCAGCTTGGGCACATATTTACTGGGC
AGCGTAGGGCAACACAACTG

Downstream 100 bases:

>100_bases
GGGTTTAAAATCGAGCAAGCAGCACCTATATCAGGGCGACATGGTTTAGCTAACCTCAGTGTCGCCCTTTTTGTTTTGTG
TACTTTTGCCTTTTATAGAG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSIREKAQEKVFHQLGRWKTLKDKNPDLIIGV
GGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPDMIDQVRRGEKAVIDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKY
CSFCVVPYTRGEEVSRPSDDIILEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICTFAELLRYVAAIDGIDRIRFTTSH
PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKARPDIQISSDFIIGFPGETKEDFAD
TMKLIEDVAFDHSFSFIYSARPGTPAADLPDDVDMEEKKQRLAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMEL
RGRTENNRVVNFEGQPKHIGSFVDVEIVDVYTNSLRGKFIRGEDEMDLRRNLRPSDILAKHKQDDDLGVTQFKP

Sequences:

>Translated_474_residues
MSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSIREKAQEKVFHQLGRWKTLKDKNPDLIIGV
GGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPDMIDQVRRGEKAVIDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKY
CSFCVVPYTRGEEVSRPSDDIILEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICTFAELLRYVAAIDGIDRIRFTTSH
PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKARPDIQISSDFIIGFPGETKEDFAD
TMKLIEDVAFDHSFSFIYSARPGTPAADLPDDVDMEEKKQRLAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMEL
RGRTENNRVVNFEGQPKHIGSFVDVEIVDVYTNSLRGKFIRGEDEMDLRRNLRPSDILAKHKQDDDLGVTQFKP
>Mature_473_residues
SKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSIREKAQEKVFHQLGRWKTLKDKNPDLIIGVG
GCVASQEGKAIKDRAQCVDIIFGPQTLHRLPDMIDQVRRGEKAVIDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYC
SFCVVPYTRGEEVSRPSDDIILEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICTFAELLRYVAAIDGIDRIRFTTSHP
IEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKARPDIQISSDFIIGFPGETKEDFADT
MKLIEDVAFDHSFSFIYSARPGTPAADLPDDVDMEEKKQRLAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMELR
GRTENNRVVNFEGQPKHIGSFVDVEIVDVYTNSLRGKFIRGEDEMDLRRNLRPSDILAKHKQDDDLGVTQFKP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=36.3070539419087, Blast_Score=288, Evalue=1e-77,
Organism=Homo sapiens, GI28872782, Length=482, Percent_Identity=36.3070539419087, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI93277076, Length=449, Percent_Identity=29.1759465478842, Blast_Score=182, Evalue=6e-46,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=77.0042194092827, Blast_Score=758, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=430, Percent_Identity=26.5116279069767, Blast_Score=144, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=462, Percent_Identity=33.5497835497836, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI71996771, Length=314, Percent_Identity=28.6624203821656, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=37.6443418013857, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI19922432, Length=458, Percent_Identity=27.7292576419214, Blast_Score=158, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 53734; Mature: 53603

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSIREKAQEKVF
CCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEECCHHHHHHHHHHH
HQLGRWKTLKDKNPDLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPDMIDQVRR
HHHHCCCCCCCCCCCEEEECCCEECCCCCCHHHHHHHHHHHEECHHHHHHHHHHHHHHHC
GEKAVIDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCSFCVVPYTRGEEVSRPSDD
CCEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHH
IILEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICTFAELLRYVAAIDGIDRIRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCC
PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKARPDI
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCEEEHHHHHHHHHHHCCCCC
QISSDFIIGFPGETKEDFADTMKLIEDVAFDHSFSFIYSARPGTPAADLPDDVDMEEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHH
RLAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMELRGRTENNRVVNFEGQPKHIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEECCCCHHCC
SFVDVEIVDVYTNSLRGKFIRGEDEMDLRRNLRPSDILAKHKQDDDLGVTQFKP
CEEEEEEEEEHHHHHCCCEECCCCHHHHHHCCCHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSIREKAQEKVF
CCEEEEEEECCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEECCHHHHHHHHHHH
HQLGRWKTLKDKNPDLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPDMIDQVRR
HHHHCCCCCCCCCCCEEEECCCEECCCCCCHHHHHHHHHHHEECHHHHHHHHHHHHHHHC
GEKAVIDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCSFCVVPYTRGEEVSRPSDD
CCEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHH
IILEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICTFAELLRYVAAIDGIDRIRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCC
PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKARPDI
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCEEEHHHHHHHHHHHCCCCC
QISSDFIIGFPGETKEDFADTMKLIEDVAFDHSFSFIYSARPGTPAADLPDDVDMEEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHH
RLAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMELRGRTENNRVVNFEGQPKHIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEECCCCHHCC
SFVDVEIVDVYTNSLRGKFIRGEDEMDLRRNLRPSDILAKHKQDDDLGVTQFKP
CEEEEEEEEEHHHHHCCCEECCCCHHHHHHCCCHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA