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The map label for this gene is mdeA [H]

Identifier: 117919379

GI number: 117919379

Start: 1087331

End: 1088623

Strand: Direct

Name: mdeA [H]

Synonym: Shewana3_0930

Alternate gene names: 117919379

Gene position: 1087331-1088623 (Clockwise)

Preceding gene: 117919378

Following gene: 117919382

Centisome position: 21.87

GC content: 51.28

Gene sequence:

>1293_bases
ATGAAACTTGAATCACTGGCATTACACCACGGATATGAATCAGAGGCGACCACGAAAGCCGCTGCGGTACCTATCTATCA
GACCACTTCCTACACCTTCGATGATACCCAACATGGTGCTGATTTATTTGACTTAAAAGTCGCAGGTAATATCTATACGC
GGATTATGAACCCGACGAGCAGCGTGCTCGAACAGCGACTTGCGGCCATTGAAGGCGGCATAGGCGCGCTGGCGCTTGCC
TCGGGAATGGCGGCCATTACCTATGCGATTCAAGCGCTTACTCAGGTGGGGGACAATATTGTCAGTACCAGTCAGCTCTA
CGGCGGCACTTATAATCTGTTTGCCCATACATTGCCGCGCCAAGGAGTCGAAGTGCGGATGGCAGCCTTCGATGATTTTG
AAGAGCTCGATGCTTTAATTGATGCTAAGACCAAGGCGCTATTCTGTGAGTCCATAGGTAACCCTGCTGGCAATATTGTT
GATCTTAAACGTTTAGCCGAGATAGCCCATAAACACGGCGTGCCTTTAATTGTCGATAACACTGTTGCCACCCCTGTTTT
ATGTCGCCCGTTTGAACATGGCGCCGATATCGTTATCCATTCGTTGACCAAATACATTGGCGGCCATGGAACGACAATTG
GCGGCGTGATTATTGACTCGGGCAAGTTCGATTGGGTGGCGAACAAAGAACGCTTTGCCCTGCTCAATCAAGCCGATCCT
TCCTACCATGGTGTGGTGTATACCGAGGCCTTTGGTGCCGCAGCCTTTATTGGACGCTGCCGCGTGGTGCCGCTGCGTAA
TACTGGGGCGGCGCTCTCACCTCACAGTGCATTTTTGCTGCTACAAGGGCTGGAAACCTTGAGTCTGCGTATGGAACGCC
ACTGCTCGAATGCCTTGGCATTGGCCGAATACTTGATACTGCATCCTTCAGTCAGCTGGGTGAATTACGGTGCTTTGCCG
AGTAGCCCTTACCGTGAAAATTGCCAAAAGATCACCGGTGGTAAAGCCTCTGGGATTATCAGCTTTGGTATTAAAGCGGC
CACTCCCGAGGAGGGTAAAATCGCTGGTGGCAAGTTTATCGACGCGCTGCAGATGATTTTGCGCTTAGTGAATATAGGTG
ATGCCAAATCGCTTGCCTGCCATCCCGCCAGCACCACTCACAGACAGTTGGACGCGAACGAGCTTGCTAGAGCCGGGGTG
TCTGAGGACTTAATTCGAATCTCCGTCGGCATTGAACATATCGACGATATTATTGCCGATGTGGCGCAGGCGCTGGAAAA
AGCCTTGGCTTAA

Upstream 100 bases:

>100_bases
CTCCCCCTCAATTTGACTTGGGGAGTGGCTATGTTTTGAGAACAGTTTAGGGAACTGTTTTTGGCGTTGTTCGGGGAATT
TATTGGGGAAGGACACAAAG

Downstream 100 bases:

>100_bases
CGTATTAACTTGATTATTCGAATGAAAAAGGCCGCTAATGTTAGCGGCCTTTTACTTTGCATAACTGACGACTTATTGGT
TTGCTTCTTGTAGGCGATTA

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKLESLALHHGYESEATTKAAAVPIYQTTSYTFDDTQHGADLFDLKVAGNIYTRIMNPTSSVLEQRLAAIEGGIGALALA
SGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPRQGVEVRMAAFDDFEELDALIDAKTKALFCESIGNPAGNIV
DLKRLAEIAHKHGVPLIVDNTVATPVLCRPFEHGADIVIHSLTKYIGGHGTTIGGVIIDSGKFDWVANKERFALLNQADP
SYHGVVYTEAFGAAAFIGRCRVVPLRNTGAALSPHSAFLLLQGLETLSLRMERHCSNALALAEYLILHPSVSWVNYGALP
SSPYRENCQKITGGKASGIISFGIKAATPEEGKIAGGKFIDALQMILRLVNIGDAKSLACHPASTTHRQLDANELARAGV
SEDLIRISVGIEHIDDIIADVAQALEKALA

Sequences:

>Translated_430_residues
MKLESLALHHGYESEATTKAAAVPIYQTTSYTFDDTQHGADLFDLKVAGNIYTRIMNPTSSVLEQRLAAIEGGIGALALA
SGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPRQGVEVRMAAFDDFEELDALIDAKTKALFCESIGNPAGNIV
DLKRLAEIAHKHGVPLIVDNTVATPVLCRPFEHGADIVIHSLTKYIGGHGTTIGGVIIDSGKFDWVANKERFALLNQADP
SYHGVVYTEAFGAAAFIGRCRVVPLRNTGAALSPHSAFLLLQGLETLSLRMERHCSNALALAEYLILHPSVSWVNYGALP
SSPYRENCQKITGGKASGIISFGIKAATPEEGKIAGGKFIDALQMILRLVNIGDAKSLACHPASTTHRQLDANELARAGV
SEDLIRISVGIEHIDDIIADVAQALEKALA
>Mature_430_residues
MKLESLALHHGYESEATTKAAAVPIYQTTSYTFDDTQHGADLFDLKVAGNIYTRIMNPTSSVLEQRLAAIEGGIGALALA
SGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPRQGVEVRMAAFDDFEELDALIDAKTKALFCESIGNPAGNIV
DLKRLAEIAHKHGVPLIVDNTVATPVLCRPFEHGADIVIHSLTKYIGGHGTTIGGVIIDSGKFDWVANKERFALLNQADP
SYHGVVYTEAFGAAAFIGRCRVVPLRNTGAALSPHSAFLLLQGLETLSLRMERHCSNALALAEYLILHPSVSWVNYGALP
SSPYRENCQKITGGKASGIISFGIKAATPEEGKIAGGKFIDALQMILRLVNIGDAKSLACHPASTTHRQLDANELARAGV
SEDLIRISVGIEHIDDIIADVAQALEKALA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=424, Percent_Identity=33.2547169811321, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI299473759, Length=424, Percent_Identity=29.7169811320755, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI25453487, Length=423, Percent_Identity=29.5508274231678, Blast_Score=133, Evalue=4e-31,
Organism=Escherichia coli, GI1790375, Length=427, Percent_Identity=30.9133489461358, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1789383, Length=426, Percent_Identity=25.3521126760563, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71980708, Length=415, Percent_Identity=33.0120481927711, Blast_Score=197, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17538055, Length=428, Percent_Identity=34.3457943925234, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17560056, Length=429, Percent_Identity=33.7995337995338, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=49.3119266055046, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6319307, Length=383, Percent_Identity=33.1592689295039, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321254, Length=450, Percent_Identity=26, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=433, Percent_Identity=27.2517321016166, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321170, Length=297, Percent_Identity=29.6296296296296, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922554, Length=428, Percent_Identity=31.7757009345794, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45929; Mature: 45929

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLESLALHHGYESEATTKAAAVPIYQTTSYTFDDTQHGADLFDLKVAGNIYTRIMNPTS
CCCHHHHHHCCCCCCCCCHHHEEEEEECCCCCCCCCCCCCCEEEEEECCCHHEEECCCHH
SVLEQRLAAIEGGIGALALASGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCC
QGVEVRMAAFDDFEELDALIDAKTKALFCESIGNPAGNIVDLKRLAEIAHKHGVPLIVDN
CCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECC
TVATPVLCRPFEHGADIVIHSLTKYIGGHGTTIGGVIIDSGKFDWVANKERFALLNQADP
CCCCCCEECCCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCCEEECCCCEEEEECCCC
SYHGVVYTEAFGAAAFIGRCRVVPLRNTGAALSPHSAFLLLQGLETLSLRMERHCSNALA
CCCCEEEEHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEYLILHPSVSWVNYGALPSSPYRENCQKITGGKASGIISFGIKAATPEEGKIAGGKFI
HHHHHHHCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCEEEECEEECCCCCCCCCCHHHH
DALQMILRLVNIGDAKSLACHPASTTHRQLDANELARAGVSEDLIRISVGIEHIDDIIAD
HHHHHHHHHHCCCCCCCEEECCCCCHHHHCCHHHHHHCCCCHHHEEEECCHHHHHHHHHH
VAQALEKALA
HHHHHHHHCC
>Mature Secondary Structure
MKLESLALHHGYESEATTKAAAVPIYQTTSYTFDDTQHGADLFDLKVAGNIYTRIMNPTS
CCCHHHHHHCCCCCCCCCHHHEEEEEECCCCCCCCCCCCCCEEEEEECCCHHEEECCCHH
SVLEQRLAAIEGGIGALALASGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCC
QGVEVRMAAFDDFEELDALIDAKTKALFCESIGNPAGNIVDLKRLAEIAHKHGVPLIVDN
CCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECC
TVATPVLCRPFEHGADIVIHSLTKYIGGHGTTIGGVIIDSGKFDWVANKERFALLNQADP
CCCCCCEECCCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCCEEECCCCEEEEECCCC
SYHGVVYTEAFGAAAFIGRCRVVPLRNTGAALSPHSAFLLLQGLETLSLRMERHCSNALA
CCCCEEEEHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEYLILHPSVSWVNYGALPSSPYRENCQKITGGKASGIISFGIKAATPEEGKIAGGKFI
HHHHHHHCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCEEEECEEECCCCCCCCCCHHHH
DALQMILRLVNIGDAKSLACHPASTTHRQLDANELARAGVSEDLIRISVGIEHIDDIIAD
HHHHHHHHHHCCCCCCCEEECCCCCHHHHCCHHHHHHCCCCHHHEEEECCHHHHHHHHHH
VAQALEKALA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]