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The map label for this gene is oprF [H]

Identifier: 117919364

GI number: 117919364

Start: 1069803

End: 1071260

Strand: Direct

Name: oprF [H]

Synonym: Shewana3_0915

Alternate gene names: 117919364

Gene position: 1069803-1071260 (Clockwise)

Preceding gene: 117919362

Following gene: 117919368

Centisome position: 21.52

GC content: 47.6

Gene sequence:

>1458_bases
ATGGGACATAAGACGTTATTGAGTGCTGTATTTTTGACTGCAACCTGCGGTGTCGCTACTTTTGCGGTGAATGCTGAATC
GCTGTTTACTCCCTTCCCGGATTCCAAATCGCATGAAGAAAAGCGCATCCACTTTACGCCCTTTGAGCTTATCACCGCAG
TGAATGGTGACAAGTTCACTCTGTTGCCTGTATCGGGGAAATTAACTCGACAAAGCGTTGAGTTACCAGAGGGGTATAGT
CCTGAACATGTGATCAATAACTATTTGGCACAGCTTAAAAAACTGAATGCTGAAGTGTTATTTAGCTGCAAGGCTGCCAC
CTGTGGTGATGGTCAAGCCATGCAAACTCAGCTAAAGCCGCTTAAGAGCGTGAATGCCGACTATCACAGTGCTTATGTGG
CGGCAAAACTGAAAGGCTCTCGTGGTGAGGTTTACACCAGTATCTATGTCGTCAATCGGGAGGATAGATACACCTATCTA
CAGGTCGATATCCTCGACGCTATCCCTGAGCCGCTGGATTTGATCCAAGCGAATAGTGACTTTTTACAGCAGGCGCCGAA
GGACATTGAGATTAAAGATAGGCGCAGTGACGACGCTCAAGGCGCGACAGACCATCCTTTGCTGGGGCGTATACCCGGCA
GTTATATTACCAGTTACAAACAAACTAACTTTACTCAAGTGCCTGTATTGGTGGGGATCTCGGGCGCAGATTATCAAACC
AAACCGCTGGACGCCAAGCTTACCCAAATAACTTATCAGATGCCTGAAAGCTACTCGTTGTTTGAAATCAACAGCAATTA
TGCCGCAGCAGCACAAAAGCTGCAGGCTGAGCGGGTGTTCCACTGTAAAGGTACGGCCTGCGGTGACGATGATAGCCTGA
TTAATGCCATCAAATTAATCAAGGATGATAAAGAAGACGAATGGCAGGAGTATCAGCTGTTTAAGCTGAGTCACGCCAAG
GGAGATGTTTACTTCGATATCTATTCCCAAGGATATTTCGATGGCACACCTGCCGATACTACGCTTAGGGTAATGGAGCT
TTCTACTCTGAAGGATGATCGTGTGGCGATTAATCTCGATGCCCTGACCGATGCGATTAGCCAAACTGGCAAGGCAACAT
TAGAGGGATTGTTGTTTGATTATGACAGTGAGCGTATGTTGCCCGAGTCTAAGCCTGTACTCGAGGTCCTCGCGAGTTAT
CTTAAGCAGAATCCGAAGCTGAGTTTTTATGTGGTCGGACATACCGATGACAAGGGCGAGCGGAGTTACAATCAAAGCTT
ATCGGAGCGCCGCGCCGCAGCAGTGATTAAGCAGTTGAGTGACGGGTTTAAAATTCCGTCAGCCCAATTAACCGCCCACG
GTAACGGGGAATACAGCCCTGTGGCCAGTAACACTAATGATGCCGGACAAAAGTTGAATCGCAGAGTGGAGTTAGTGCTT
CGTTCCGATAAAAAGTAG

Upstream 100 bases:

>100_bases
ATACCTAGGTAAATATTTTTATCGTATTATCCCGTCACTATTTTTTATCTAGTTAGTTGGTTCGCGAGGGCTAACTTTCC
CCCACAGAAAAGGATTTTTT

Downstream 100 bases:

>100_bases
CTCTATTTCATCAGCTTGAAAGGCTACTTTAGCTTCATTAACAACGCCTGAGCCATCTCAGGCGTTTTTACTGGTATTGG
CGTTCATCGGAAGATATTGT

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MGHKTLLSAVFLTATCGVATFAVNAESLFTPFPDSKSHEEKRIHFTPFELITAVNGDKFTLLPVSGKLTRQSVELPEGYS
PEHVINNYLAQLKKLNAEVLFSCKAATCGDGQAMQTQLKPLKSVNADYHSAYVAAKLKGSRGEVYTSIYVVNREDRYTYL
QVDILDAIPEPLDLIQANSDFLQQAPKDIEIKDRRSDDAQGATDHPLLGRIPGSYITSYKQTNFTQVPVLVGISGADYQT
KPLDAKLTQITYQMPESYSLFEINSNYAAAAQKLQAERVFHCKGTACGDDDSLINAIKLIKDDKEDEWQEYQLFKLSHAK
GDVYFDIYSQGYFDGTPADTTLRVMELSTLKDDRVAINLDALTDAISQTGKATLEGLLFDYDSERMLPESKPVLEVLASY
LKQNPKLSFYVVGHTDDKGERSYNQSLSERRAAAVIKQLSDGFKIPSAQLTAHGNGEYSPVASNTNDAGQKLNRRVELVL
RSDKK

Sequences:

>Translated_485_residues
MGHKTLLSAVFLTATCGVATFAVNAESLFTPFPDSKSHEEKRIHFTPFELITAVNGDKFTLLPVSGKLTRQSVELPEGYS
PEHVINNYLAQLKKLNAEVLFSCKAATCGDGQAMQTQLKPLKSVNADYHSAYVAAKLKGSRGEVYTSIYVVNREDRYTYL
QVDILDAIPEPLDLIQANSDFLQQAPKDIEIKDRRSDDAQGATDHPLLGRIPGSYITSYKQTNFTQVPVLVGISGADYQT
KPLDAKLTQITYQMPESYSLFEINSNYAAAAQKLQAERVFHCKGTACGDDDSLINAIKLIKDDKEDEWQEYQLFKLSHAK
GDVYFDIYSQGYFDGTPADTTLRVMELSTLKDDRVAINLDALTDAISQTGKATLEGLLFDYDSERMLPESKPVLEVLASY
LKQNPKLSFYVVGHTDDKGERSYNQSLSERRAAAVIKQLSDGFKIPSAQLTAHGNGEYSPVASNTNDAGQKLNRRVELVL
RSDKK
>Mature_484_residues
GHKTLLSAVFLTATCGVATFAVNAESLFTPFPDSKSHEEKRIHFTPFELITAVNGDKFTLLPVSGKLTRQSVELPEGYSP
EHVINNYLAQLKKLNAEVLFSCKAATCGDGQAMQTQLKPLKSVNADYHSAYVAAKLKGSRGEVYTSIYVVNREDRYTYLQ
VDILDAIPEPLDLIQANSDFLQQAPKDIEIKDRRSDDAQGATDHPLLGRIPGSYITSYKQTNFTQVPVLVGISGADYQTK
PLDAKLTQITYQMPESYSLFEINSNYAAAAQKLQAERVFHCKGTACGDDDSLINAIKLIKDDKEDEWQEYQLFKLSHAKG
DVYFDIYSQGYFDGTPADTTLRVMELSTLKDDRVAINLDALTDAISQTGKATLEGLLFDYDSERMLPESKPVLEVLASYL
KQNPKLSFYVVGHTDDKGERSYNQSLSERRAAAVIKQLSDGFKIPSAQLTAHGNGEYSPVASNTNDAGQKLNRRVELVLR
SDKK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1788957, Length=106, Percent_Identity=36.7924528301887, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 53819; Mature: 53688

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHKTLLSAVFLTATCGVATFAVNAESLFTPFPDSKSHEEKRIHFTPFELITAVNGDKFT
CCHHHHHHHHHHHHHCCCEEEEECHHHHCCCCCCCCCCCCCEEEECHHHHHEECCCCEEE
LLPVSGKLTRQSVELPEGYSPEHVINNYLAQLKKLNAEVLFSCKAATCGDGQAMQTQLKP
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHH
LKSVNADYHSAYVAAKLKGSRGEVYTSIYVVNREDRYTYLQVDILDAIPEPLDLIQANSD
HHHCCCCHHHEEEEEEECCCCCCEEEEEEEEECCCCEEEEEEEEHHHCCCCHHHHCCCCH
FLQQAPKDIEIKDRRSDDAQGATDHPLLGRIPGSYITSYKQTNFTQVPVLVGISGADYQT
HHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEECEEEEEECCCCCCC
KPLDAKLTQITYQMPESYSLFEINSNYAAAAQKLQAERVFHCKGTACGDDDSLINAIKLI
CCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHH
KDDKEDEWQEYQLFKLSHAKGDVYFDIYSQGYFDGTPADTTLRVMELSTLKDDRVAINLD
HCCCCCHHHHEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCEEEEHH
ALTDAISQTGKATLEGLLFDYDSERMLPESKPVLEVLASYLKQNPKLSFYVVGHTDDKGE
HHHHHHHHCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCH
RSYNQSLSERRAAAVIKQLSDGFKIPSAQLTAHGNGEYSPVASNTNDAGQKLNRRVELVL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHEEEEE
RSDKK
ECCCC
>Mature Secondary Structure 
GHKTLLSAVFLTATCGVATFAVNAESLFTPFPDSKSHEEKRIHFTPFELITAVNGDKFT
CHHHHHHHHHHHHHCCCEEEEECHHHHCCCCCCCCCCCCCEEEECHHHHHEECCCCEEE
LLPVSGKLTRQSVELPEGYSPEHVINNYLAQLKKLNAEVLFSCKAATCGDGQAMQTQLKP
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHH
LKSVNADYHSAYVAAKLKGSRGEVYTSIYVVNREDRYTYLQVDILDAIPEPLDLIQANSD
HHHCCCCHHHEEEEEEECCCCCCEEEEEEEEECCCCEEEEEEEEHHHCCCCHHHHCCCCH
FLQQAPKDIEIKDRRSDDAQGATDHPLLGRIPGSYITSYKQTNFTQVPVLVGISGADYQT
HHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEECEEEEEECCCCCCC
KPLDAKLTQITYQMPESYSLFEINSNYAAAAQKLQAERVFHCKGTACGDDDSLINAIKLI
CCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHH
KDDKEDEWQEYQLFKLSHAKGDVYFDIYSQGYFDGTPADTTLRVMELSTLKDDRVAINLD
HCCCCCHHHHEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCEEEEHH
ALTDAISQTGKATLEGLLFDYDSERMLPESKPVLEVLASYLKQNPKLSFYVVGHTDDKGE
HHHHHHHHCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCH
RSYNQSLSERRAAAVIKQLSDGFKIPSAQLTAHGNGEYSPVASNTNDAGQKLNRRVELVL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHEEEEE
RSDKK
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]