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The map label for this gene is pldA [H]

Identifier: 117919313

GI number: 117919313

Start: 1003095

End: 1003979

Strand: Reverse

Name: pldA [H]

Synonym: Shewana3_0861

Alternate gene names: 117919313

Gene position: 1003979-1003095 (Counterclockwise)

Preceding gene: 117919319

Following gene: 117919312

Centisome position: 20.19

GC content: 45.31

Gene sequence:

>885_bases
ATGTCCCTATTTTATAAAGGTATCGCATTAATTGGACTGTTAACTTGTACAGGACTTCAAGCGGAAGAATCCCTCGTTAA
AGGACGGGTACAGGACGAGCTAGCCACCTCAGAACGTCCATTCGTGATCACGCCGCATAAAGTGAATTACATTTTACCCG
CCACTTACAACCCCGATCCCAATATGGCGCCCTTCGCCCAAGATGCGGCCGAAAACGACTATACCCTGGATGAAATGGAA
GCTAAGTTCCAGATAAGTTTTAAATTTCCTCTTTGGTATAATGTCTTTGGCGATAATGGCCACCTGTTTTTTGCCTACAC
CAACCAGTCCTATTGGCAGCTTTACAACAAGGATATTTCTTCCCCCTTCCGTGAGACTAACCACGAACCCGAAATCTTTA
TGCTGTTCAACAACGATTGGAAAATCGGCAGCGTGACTAACTCCTTCTGGGGCTTTGGCGCCGTGCATCAATCTAATGGT
AAGTCAGGGTTATTATCCCGTAGCTGGAACCGTATCTATGGCACCATGATCTTCGATGCCGGCCCGCTGGCCTTTGCCAC
TAAGGTGTGGTGGCGTATTCCAGAGGACGAAAAAACCGATATCCATCAACCCCGCGGTGACGATAACCCCGATATTGATG
AATACATAGGTAAAGCCGAGTTTGTAGGGGTTTATGGCATTGATGACCATAGATTCACCCTCACCCTAAAAACTAACTTT
AAAGATATTGATCGAGGATCGGCAGAATTTACATGGAGCTATCCGATAATCGGTAATTTACGCCTTTATACTCAATACTT
TAACGGCTATGGCGAGAGTCTTATCGACTACAACTACCATAACCAACGCATAGGTATCGGCCTATCACTCAACGATATTC
TCTAA

Upstream 100 bases:

>100_bases
AGACACATGGCAGGATTTCTCATAATAAATCCACAAACTAATGTTAAGCTGGGTAAACAACGAGAAAGCGTAAGGCAAAC
AGACACATCAAGGAGGGCCG

Downstream 100 bases:

>100_bases
CGCTACAACCGTGACAGAGCACAAAAAACAGCCATAATTCTATGCTGACTTGCAAAAACTGCTGCTAAATTGCTCAAATG
GATACGAGCAAAAGGTGAGC

Product: phospholipase A1

Products: NA

Alternate protein names: Detergent-resistant phospholipase A; DR-phospholipase A; Outer membrane phospholipase A; OM PLA; OMPLA; Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSLFYKGIALIGLLTCTGLQAEESLVKGRVQDELATSERPFVITPHKVNYILPATYNPDPNMAPFAQDAAENDYTLDEME
AKFQISFKFPLWYNVFGDNGHLFFAYTNQSYWQLYNKDISSPFRETNHEPEIFMLFNNDWKIGSVTNSFWGFGAVHQSNG
KSGLLSRSWNRIYGTMIFDAGPLAFATKVWWRIPEDEKTDIHQPRGDDNPDIDEYIGKAEFVGVYGIDDHRFTLTLKTNF
KDIDRGSAEFTWSYPIIGNLRLYTQYFNGYGESLIDYNYHNQRIGIGLSLNDIL

Sequences:

>Translated_294_residues
MSLFYKGIALIGLLTCTGLQAEESLVKGRVQDELATSERPFVITPHKVNYILPATYNPDPNMAPFAQDAAENDYTLDEME
AKFQISFKFPLWYNVFGDNGHLFFAYTNQSYWQLYNKDISSPFRETNHEPEIFMLFNNDWKIGSVTNSFWGFGAVHQSNG
KSGLLSRSWNRIYGTMIFDAGPLAFATKVWWRIPEDEKTDIHQPRGDDNPDIDEYIGKAEFVGVYGIDDHRFTLTLKTNF
KDIDRGSAEFTWSYPIIGNLRLYTQYFNGYGESLIDYNYHNQRIGIGLSLNDIL
>Mature_293_residues
SLFYKGIALIGLLTCTGLQAEESLVKGRVQDELATSERPFVITPHKVNYILPATYNPDPNMAPFAQDAAENDYTLDEMEA
KFQISFKFPLWYNVFGDNGHLFFAYTNQSYWQLYNKDISSPFRETNHEPEIFMLFNNDWKIGSVTNSFWGFGAVHQSNGK
SGLLSRSWNRIYGTMIFDAGPLAFATKVWWRIPEDEKTDIHQPRGDDNPDIDEYIGKAEFVGVYGIDDHRFTLTLKTNFK
DIDRGSAEFTWSYPIIGNLRLYTQYFNGYGESLIDYNYHNQRIGIGLSLNDIL

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=271, Percent_Identity=38.3763837638376, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 33841; Mature: 33710

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFYKGIALIGLLTCTGLQAEESLVKGRVQDELATSERPFVITPHKVNYILPATYNPDP
CCHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHCCCCCEEECCCCEEEEEEECCCCCC
NMAPFAQDAAENDYTLDEMEAKFQISFKFPLWYNVFGDNGHLFFAYTNQSYWQLYNKDIS
CCCCCHHHCCCCCCCHHCCCEEEEEEEECCEEEEEECCCCEEEEEECCCHHHHHHHHHHC
SPFRETNHEPEIFMLFNNDWKIGSVTNSFWGFGAVHQSNGKSGLLSRSWNRIYGTMIFDA
CHHHHCCCCCEEEEEECCCEEECCCCCCCCCCCEEECCCCCCCCHHCCCCCEEEEEEECC
GPLAFATKVWWRIPEDEKTDIHQPRGDDNPDIDEYIGKAEFVGVYGIDDHRFTLTLKTNF
CCHHEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCEEEEEEEECCCCEEEEEEECCC
KDIDRGSAEFTWSYPIIGNLRLYTQYFNGYGESLIDYNYHNQRIGIGLSLNDIL
CCCCCCCCEEEEECCEECCEEEEEEHHCCCCCCEEECCCCCCEEEEEEEHHHCC
>Mature Secondary Structure 
SLFYKGIALIGLLTCTGLQAEESLVKGRVQDELATSERPFVITPHKVNYILPATYNPDP
CHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHCCCCCEEECCCCEEEEEEECCCCCC
NMAPFAQDAAENDYTLDEMEAKFQISFKFPLWYNVFGDNGHLFFAYTNQSYWQLYNKDIS
CCCCCHHHCCCCCCCHHCCCEEEEEEEECCEEEEEECCCCEEEEEECCCHHHHHHHHHHC
SPFRETNHEPEIFMLFNNDWKIGSVTNSFWGFGAVHQSNGKSGLLSRSWNRIYGTMIFDA
CHHHHCCCCCEEEEEECCCEEECCCCCCCCCCCEEECCCCCCCCHHCCCCCEEEEEEECC
GPLAFATKVWWRIPEDEKTDIHQPRGDDNPDIDEYIGKAEFVGVYGIDDHRFTLTLKTNF
CCHHEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCEEEEEEEECCCCEEEEEEECCC
KDIDRGSAEFTWSYPIIGNLRLYTQYFNGYGESLIDYNYHNQRIGIGLSLNDIL
CCCCCCCCEEEEECCEECCEEEEEEHHCCCCCCEEECCCCCCEEEEEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]