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The map label for this gene is cysI [H]

Identifier: 117919308

GI number: 117919308

Start: 995254

End: 996951

Strand: Direct

Name: cysI [H]

Synonym: Shewana3_0856

Alternate gene names: 117919308

Gene position: 995254-996951 (Clockwise)

Preceding gene: 117919307

Following gene: 117919309

Centisome position: 20.02

GC content: 50.82

Gene sequence:

>1698_bases
ATGAGTGAGCAAAAGTTAGCATTAAACGAATACCTAAAGACCGACAGTGATTATCTGCGTGGCACCATTAAAGAGGGATT
AGATTCCTCGGTGACGGGCAGTTTTAGCGATGGCGATCAGCAATTGATCAAATTCCACGGCTTCTATCAGCAGGATGACC
GTGATTTACGTAACGAGCGTAAAGAGCAAAAACTCGAACCCTTATATAGCTTTATGTTACGTGCCCGCGTGCCTGGCGGT
GTGTGTACGCCTAAGCAATGGTTAGGCGTGGATGAAATTGCCTCGACGCTCACCAGCTCTAACAGCATTCGTTTAACGAC
GCGTCAGACGTTCCAATACCACGGCATTCCAAAGCGTAACCTCAAGACCATTATTCAAGGCTTGGACCGTGAGGCACTCG
ATTCTATCGCCGCCTGTGGTGACGTTAACCGTAACGTGATGTGTAACCCGAATCCGGTCGAATCTAAGCTGCATGCGCAG
GCCTATGAAGTCGCGAAAAAGCTGTCTGATCACCTGCTGCCCCACACTCGTGCCTACGCCGAGATTTGGTTAGATGAAGA
GAAGTTACTGACCACAGAAGACGAAACCGTTGAACCTGTATACGGCAAAACCTACTTGCCACGTAAGTTTAAGATGGCCG
TTGCCGTACCGCCGGATAACGATGTTGATGTCTACACCAACGACTTAGGCTTTATTGCGGTGGCCGAAAATGGCGAATTA
GTCGGTTTTAACCTGACCGCTGGCGGCGGCATGGGCTCGACCCACGGTGAAGTCGAAACCTTCCCGCGCTTGGCCGATGA
CTTTGGTTTTATCAAAACTGAAGATGTGATGAAGTTTGCCGAAGCCGTGATGACAGTGCAGCGTGACTGGGGTAATCGTA
CCAACCGTAAACGTTCACGCCTTAAATACACTATCGTTGACCATGGTTATGAGAAGTTTAAGGCCGAAGTGGAAGCCCGT
GCTGGCGTCAAATTTGAGCCTAAGCGTGATGTGGTGATTGGCGATCGCGGCGATCGTTACGGCTGGGTCGAAGGTGTCGA
TGGTAAGTGGCATTTAACCCTGTTTATCGAAAGCGGCCGCATTAAAGATGTGCCCGGTAAGAGTTTGCAAACAGGTATGC
GTGAAATCGCGAAGATCCACAAGGGCGATTTCCGCATGACCTCAAACCAGAATATGATTATTGCTGGTGTGGCGCCTGAA
GATAAAGCGACCATCGAAGGCCTTGCCCGTAAACACGGTTTACTCGGCCAAGTGCTGACCCAAACCCGCGGTCATTCGAT
TGCCTGTGTGGCCTTGCCAACCTGTCCATTGGCGATGGCGGAAGCTGAGCGTTATTTCCCTGAGTTTATTGACCATATCG
ATGCGCTGCAGGCTAAAAATGGCATTAGCGATCAAGCCATTGTGGTGCGTATGACCGGCTGTCCAAATGGTTGCGCCCGT
CCATTTGCCGCCGAAATCGGCTTAGTGGGTAAGGCGCCGGGTCGCTATAACCTGTATCTCGGGGCGAACTTCGAAGGTAC
TCGTCTGAATAAGATGTACAGAGAGAATATCCAAGAGGCTGAGATCCTTGCTGAATTGGATGCATTATTTGCCCGCTACG
CGGTTGAGCGTAATGCAGGCGAAACCTTTGGTAACTTCACCGTCCGCACGGGCGTGGTGAAGGCGGTTATCGATGCCGCT
AAGGATTTCCATGGCTGA

Upstream 100 bases:

>100_bases
AACGTTACCAAAAAGACGTGTACTAATTAATCGATCCCAAAGATAGAGAGTAGTATCCACTGAGATACTGCCGCCGAACG
AATTTTAAGAGGCAGTTGCC

Downstream 100 bases:

>100_bases
TCTTGATTCTGGGGTAACGGGTTCTGGCTTGCCGAACGCGTTGCAGAGCGTGGTAAGTCACAGCGAACTTAAAGCGCTGC
TGACGGCGCCTAAGGATGTG

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP [H]

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MSEQKLALNEYLKTDSDYLRGTIKEGLDSSVTGSFSDGDQQLIKFHGFYQQDDRDLRNERKEQKLEPLYSFMLRARVPGG
VCTPKQWLGVDEIASTLTSSNSIRLTTRQTFQYHGIPKRNLKTIIQGLDREALDSIAACGDVNRNVMCNPNPVESKLHAQ
AYEVAKKLSDHLLPHTRAYAEIWLDEEKLLTTEDETVEPVYGKTYLPRKFKMAVAVPPDNDVDVYTNDLGFIAVAENGEL
VGFNLTAGGGMGSTHGEVETFPRLADDFGFIKTEDVMKFAEAVMTVQRDWGNRTNRKRSRLKYTIVDHGYEKFKAEVEAR
AGVKFEPKRDVVIGDRGDRYGWVEGVDGKWHLTLFIESGRIKDVPGKSLQTGMREIAKIHKGDFRMTSNQNMIIAGVAPE
DKATIEGLARKHGLLGQVLTQTRGHSIACVALPTCPLAMAEAERYFPEFIDHIDALQAKNGISDQAIVVRMTGCPNGCAR
PFAAEIGLVGKAPGRYNLYLGANFEGTRLNKMYRENIQEAEILAELDALFARYAVERNAGETFGNFTVRTGVVKAVIDAA
KDFHG

Sequences:

>Translated_565_residues
MSEQKLALNEYLKTDSDYLRGTIKEGLDSSVTGSFSDGDQQLIKFHGFYQQDDRDLRNERKEQKLEPLYSFMLRARVPGG
VCTPKQWLGVDEIASTLTSSNSIRLTTRQTFQYHGIPKRNLKTIIQGLDREALDSIAACGDVNRNVMCNPNPVESKLHAQ
AYEVAKKLSDHLLPHTRAYAEIWLDEEKLLTTEDETVEPVYGKTYLPRKFKMAVAVPPDNDVDVYTNDLGFIAVAENGEL
VGFNLTAGGGMGSTHGEVETFPRLADDFGFIKTEDVMKFAEAVMTVQRDWGNRTNRKRSRLKYTIVDHGYEKFKAEVEAR
AGVKFEPKRDVVIGDRGDRYGWVEGVDGKWHLTLFIESGRIKDVPGKSLQTGMREIAKIHKGDFRMTSNQNMIIAGVAPE
DKATIEGLARKHGLLGQVLTQTRGHSIACVALPTCPLAMAEAERYFPEFIDHIDALQAKNGISDQAIVVRMTGCPNGCAR
PFAAEIGLVGKAPGRYNLYLGANFEGTRLNKMYRENIQEAEILAELDALFARYAVERNAGETFGNFTVRTGVVKAVIDAA
KDFHG
>Mature_564_residues
SEQKLALNEYLKTDSDYLRGTIKEGLDSSVTGSFSDGDQQLIKFHGFYQQDDRDLRNERKEQKLEPLYSFMLRARVPGGV
CTPKQWLGVDEIASTLTSSNSIRLTTRQTFQYHGIPKRNLKTIIQGLDREALDSIAACGDVNRNVMCNPNPVESKLHAQA
YEVAKKLSDHLLPHTRAYAEIWLDEEKLLTTEDETVEPVYGKTYLPRKFKMAVAVPPDNDVDVYTNDLGFIAVAENGELV
GFNLTAGGGMGSTHGEVETFPRLADDFGFIKTEDVMKFAEAVMTVQRDWGNRTNRKRSRLKYTIVDHGYEKFKAEVEARA
GVKFEPKRDVVIGDRGDRYGWVEGVDGKWHLTLFIESGRIKDVPGKSLQTGMREIAKIHKGDFRMTSNQNMIIAGVAPED
KATIEGLARKHGLLGQVLTQTRGHSIACVALPTCPLAMAEAERYFPEFIDHIDALQAKNGISDQAIVVRMTGCPNGCARP
FAAEIGLVGKAPGRYNLYLGANFEGTRLNKMYRENIQEAEILAELDALFARYAVERNAGETFGNFTVRTGVVKAVIDAAK
DFHG

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate [H]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=561, Percent_Identity=59.1800356506239, Blast_Score=700, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322597, Length=593, Percent_Identity=44.0134907251265, Blast_Score=530, Evalue=1e-151,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 62972; Mature: 62841

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQKLALNEYLKTDSDYLRGTIKEGLDSSVTGSFSDGDQQLIKFHGFYQQDDRDLRNER
CCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
KEQKLEPLYSFMLRARVPGGVCTPKQWLGVDEIASTLTSSNSIRLTTRQTFQYHGIPKRN
HHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHCCCCCEEEEEEEEEEECCCCHHH
LKTIIQGLDREALDSIAACGDVNRNVMCNPNPVESKLHAQAYEVAKKLSDHLLPHTRAYA
HHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EIWLDEEKLLTTEDETVEPVYGKTYLPRKFKMAVAVPPDNDVDVYTNDLGFIAVAENGEL
HHEECCCCEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCEEEEEECCCCE
VGFNLTAGGGMGSTHGEVETFPRLADDFGFIKTEDVMKFAEAVMTVQRDWGNRTNRKRSR
EEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LKYTIVDHGYEKFKAEVEARAGVKFEPKRDVVIGDRGDRYGWVEGVDGKWHLTLFIESGR
EEEEEECCCHHHHHHHHHHHCCCEECCCCCEEECCCCCCCCCEECCCCEEEEEEEEECCC
IKDVPGKSLQTGMREIAKIHKGDFRMTSNQNMIIAGVAPEDKATIEGLARKHGLLGQVLT
CCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
QTRGHSIACVALPTCPLAMAEAERYFPEFIDHIDALQAKNGISDQAIVVRMTGCPNGCAR
HCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
PFAAEIGLVGKAPGRYNLYLGANFEGTRLNKMYRENIQEAEILAELDALFARYAVERNAG
CHHHHCCCEECCCCCEEEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCC
ETFGNFTVRTGVVKAVIDAAKDFHG
CCCCCEEHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEQKLALNEYLKTDSDYLRGTIKEGLDSSVTGSFSDGDQQLIKFHGFYQQDDRDLRNER
CCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
KEQKLEPLYSFMLRARVPGGVCTPKQWLGVDEIASTLTSSNSIRLTTRQTFQYHGIPKRN
HHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHCCCCCEEEEEEEEEEECCCCHHH
LKTIIQGLDREALDSIAACGDVNRNVMCNPNPVESKLHAQAYEVAKKLSDHLLPHTRAYA
HHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EIWLDEEKLLTTEDETVEPVYGKTYLPRKFKMAVAVPPDNDVDVYTNDLGFIAVAENGEL
HHEECCCCEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCEEEEEECCCCE
VGFNLTAGGGMGSTHGEVETFPRLADDFGFIKTEDVMKFAEAVMTVQRDWGNRTNRKRSR
EEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LKYTIVDHGYEKFKAEVEARAGVKFEPKRDVVIGDRGDRYGWVEGVDGKWHLTLFIESGR
EEEEEECCCHHHHHHHHHHHCCCEECCCCCEEECCCCCCCCCEECCCCEEEEEEEEECCC
IKDVPGKSLQTGMREIAKIHKGDFRMTSNQNMIIAGVAPEDKATIEGLARKHGLLGQVLT
CCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
QTRGHSIACVALPTCPLAMAEAERYFPEFIDHIDALQAKNGISDQAIVVRMTGCPNGCAR
HCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
PFAAEIGLVGKAPGRYNLYLGANFEGTRLNKMYRENIQEAEILAELDALFARYAVERNAG
CHHHHCCCEECCCCCEEEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCC
ETFGNFTVRTGVVKAVIDAAKDFHG
CCCCCEEHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA