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The map label for this gene is cysJ [H]

Identifier: 117919307

GI number: 117919307

Start: 993365

End: 995179

Strand: Direct

Name: cysJ [H]

Synonym: Shewana3_0855

Alternate gene names: 117919307

Gene position: 993365-995179 (Clockwise)

Preceding gene: 117919303

Following gene: 117919308

Centisome position: 19.98

GC content: 51.29

Gene sequence:

>1815_bases
ATGTTGTTAAAAGAACTTTCATCACTGGCTTCACCCCTGTCGCAACCGCAGGTCGAAAAGCTGAAACAACTCACCGCTGA
ATTAAACGCGGTGCAACTTGCTTGGGTGAGCGGTTATTTAGCCGCAACGGCCAATGCGTCGGGTAATCTAGCCCAGCTCG
CGCCCGTGTCCGATGCACCGGCGGCGCAGACAGTGACCATTCTCTATGGCAGCCAAACCGGCAATGGTCGTGGTATTGCC
AAAGCCTTGGCCGAGAAGGCAAAAGCGCAGGGCTATAGCGTGAATCTAGCCTCTATGGGTGAATACAATGTGCGCCAGCT
TAAACAAGAAACCTTGTTATTGCTGGTGGTCAGTACCCATGGTGAGGGGGAAGCGCCGGATGATGCGATTGAATTACATA
AGTTTTTAGCATCGAAACGCGCACCTCAATTAAACAACTTACATTACTCTGTGTTGGCGCTGGGCGATTCAAGCTATGAG
TTTTTCTGCCAGACAGGTAAAGACTTCGATGCTCGTCTGTCGGCATTAGGCGCCAAAGCGCTGCTGCCTCTGGTTGAGTG
TGATGTGGACTATGAGGCTGCGGCCGGACAGTGGCACGCCGATGTACTCACCGCCGTGAAGCCACTGATTCAAACCACGG
CTAACGTGGTGGCGCTCAATGATACCGGCTCGGCACAAGTTGCCAGCGAAAGCGAATTTACTAAGCAAAACCCTTACAGT
GCTGAGGTGTTGGTGAGCCAGAAGATCACTGGACGCGGTTCAGACCGAGACGTACGCCACGTTGAAATCGATTTAGGCGA
GTCAGGCTTACGCTACGAAGTGGGTGATGCCCTTGGGGTGTGGTTTAGCAACAATGAAACTTTGGTCGATGAGATTTTAG
CGGGCTTAGGGTTAGCGGCCGATGCCACTGTGACCGTCGGTAATGAGTCAATCAACCTCAAACAAGCCTTAATCGAGAAG
AAAGAGCTGACACAGTTATATCCTGGTTTAGTCAAAGCCTGGGCCGAGTTGTCCGCAAGCCCTGAGCTGCTTGCTATTAG
CGAAGATAAAGAACAGGTTCGCCAGTTTATCTTACATCATCAATTTGCAGACTTAGTGGCGAACTATCAGCTCAAGGCCG
ATGCTAATCTGGATGCAAACAAGTTAGTGGAGCTATTACGCCCATTAACGCCAAGACTCTACTCCATCGCTTCGAGCCAG
AGTGAAGTCGATACCGAAGTGCATCTGACAGTGGCCTTGGTGGAGGATGAGCACCAAGGGCAAGCCCGTTTTGGCGGCGC
ATCGCATTTCTTAGCCTCGGCAGAGGAAGGCGCCGAAGTAAAAGTGTATGTTGAACCGAACAAGCATTTCCGTTTACCTG
AAGATCCACAAACGCCTGTCATCATGATTGGCCCAGGCACTGGTGTTGCGCCGTTTCGCGCCTTTATGCAGGAACGCGTG
GCGCAAGGCGCTGAGGGCGATAGCTGGTTATTCTTCGGTAATCCTCACTTTGAGCAGGACTTCCTGTATCAAACCGAGTG
GCAACAGTATCTGAAAAACGGCGATTTAACCCGTATCGATGTGGCATTTTCGCGCGATCAGGCTCATAAGATTTACGTGC
AGCACCGCATTAAAGAGCAGGGACAAACCCTGTGGCAATGGCTGCAAAATGGCGCGCATCTTTATATTTGTGGTGATGCC
GAGCGTATGGCTAAAGACGTACATCAAGCCCTATTAGCGGTTGCGGTGGAGTTTGGCGGACTGTCGAGCGAGGCGGCGGA
AGAATATTTCGAGACTCTGCGCAGTCATAAACGTTACCAAAAAGACGTGTACTAA

Upstream 100 bases:

>100_bases
TGTATATTCCAAAATGGAATTAAAAATCAAATATTATTCTTTTTTGCTTTTCTAAAACCACGCTAATCTGCTGGACATTA
CACTGTCATAGGGCCGTCTC

Downstream 100 bases:

>100_bases
TTAATCGATCCCAAAGATAGAGAGTAGTATCCACTGAGATACTGCCGCCGAACGAATTTTAAGAGGCAGTTGCCATGAGT
GAGCAAAAGTTAGCATTAAA

Product: sulfite reductase (NADPH) subunit alpha

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MLLKELSSLASPLSQPQVEKLKQLTAELNAVQLAWVSGYLAATANASGNLAQLAPVSDAPAAQTVTILYGSQTGNGRGIA
KALAEKAKAQGYSVNLASMGEYNVRQLKQETLLLLVVSTHGEGEAPDDAIELHKFLASKRAPQLNNLHYSVLALGDSSYE
FFCQTGKDFDARLSALGAKALLPLVECDVDYEAAAGQWHADVLTAVKPLIQTTANVVALNDTGSAQVASESEFTKQNPYS
AEVLVSQKITGRGSDRDVRHVEIDLGESGLRYEVGDALGVWFSNNETLVDEILAGLGLAADATVTVGNESINLKQALIEK
KELTQLYPGLVKAWAELSASPELLAISEDKEQVRQFILHHQFADLVANYQLKADANLDANKLVELLRPLTPRLYSIASSQ
SEVDTEVHLTVALVEDEHQGQARFGGASHFLASAEEGAEVKVYVEPNKHFRLPEDPQTPVIMIGPGTGVAPFRAFMQERV
AQGAEGDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLTRIDVAFSRDQAHKIYVQHRIKEQGQTLWQWLQNGAHLYICGDA
ERMAKDVHQALLAVAVEFGGLSSEAAEEYFETLRSHKRYQKDVY

Sequences:

>Translated_604_residues
MLLKELSSLASPLSQPQVEKLKQLTAELNAVQLAWVSGYLAATANASGNLAQLAPVSDAPAAQTVTILYGSQTGNGRGIA
KALAEKAKAQGYSVNLASMGEYNVRQLKQETLLLLVVSTHGEGEAPDDAIELHKFLASKRAPQLNNLHYSVLALGDSSYE
FFCQTGKDFDARLSALGAKALLPLVECDVDYEAAAGQWHADVLTAVKPLIQTTANVVALNDTGSAQVASESEFTKQNPYS
AEVLVSQKITGRGSDRDVRHVEIDLGESGLRYEVGDALGVWFSNNETLVDEILAGLGLAADATVTVGNESINLKQALIEK
KELTQLYPGLVKAWAELSASPELLAISEDKEQVRQFILHHQFADLVANYQLKADANLDANKLVELLRPLTPRLYSIASSQ
SEVDTEVHLTVALVEDEHQGQARFGGASHFLASAEEGAEVKVYVEPNKHFRLPEDPQTPVIMIGPGTGVAPFRAFMQERV
AQGAEGDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLTRIDVAFSRDQAHKIYVQHRIKEQGQTLWQWLQNGAHLYICGDA
ERMAKDVHQALLAVAVEFGGLSSEAAEEYFETLRSHKRYQKDVY
>Mature_604_residues
MLLKELSSLASPLSQPQVEKLKQLTAELNAVQLAWVSGYLAATANASGNLAQLAPVSDAPAAQTVTILYGSQTGNGRGIA
KALAEKAKAQGYSVNLASMGEYNVRQLKQETLLLLVVSTHGEGEAPDDAIELHKFLASKRAPQLNNLHYSVLALGDSSYE
FFCQTGKDFDARLSALGAKALLPLVECDVDYEAAAGQWHADVLTAVKPLIQTTANVVALNDTGSAQVASESEFTKQNPYS
AEVLVSQKITGRGSDRDVRHVEIDLGESGLRYEVGDALGVWFSNNETLVDEILAGLGLAADATVTVGNESINLKQALIEK
KELTQLYPGLVKAWAELSASPELLAISEDKEQVRQFILHHQFADLVANYQLKADANLDANKLVELLRPLTPRLYSIASSQ
SEVDTEVHLTVALVEDEHQGQARFGGASHFLASAEEGAEVKVYVEPNKHFRLPEDPQTPVIMIGPGTGVAPFRAFMQERV
AQGAEGDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLTRIDVAFSRDQAHKIYVQHRIKEQGQTLWQWLQNGAHLYICGDA
ERMAKDVHQALLAVAVEFGGLSSEAAEEYFETLRSHKRYQKDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI7657393, Length=602, Percent_Identity=31.5614617940199, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI221316709, Length=602, Percent_Identity=31.5614617940199, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI221316705, Length=611, Percent_Identity=31.0965630114566, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI127139033, Length=607, Percent_Identity=30.9719934102142, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI221316707, Length=532, Percent_Identity=31.5789473684211, Blast_Score=224, Evalue=2e-58,
Organism=Homo sapiens, GI24041029, Length=597, Percent_Identity=30.6532663316583, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI10835173, Length=642, Percent_Identity=27.5700934579439, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI40254422, Length=646, Percent_Identity=26.9349845201238, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI169790956, Length=479, Percent_Identity=27.9749478079332, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI169790958, Length=479, Percent_Identity=27.9749478079332, Blast_Score=149, Evalue=1e-35,
Organism=Escherichia coli, GI1789123, Length=599, Percent_Identity=50.7512520868113, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1789154, Length=109, Percent_Identity=35.7798165137615, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17554134, Length=593, Percent_Identity=30.6913996627319, Blast_Score=224, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17566446, Length=580, Percent_Identity=28.7931034482759, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17531441, Length=413, Percent_Identity=24.2130750605327, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32563863, Length=125, Percent_Identity=37.6, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321832, Length=665, Percent_Identity=29.3233082706767, Blast_Score=216, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6321143, Length=391, Percent_Identity=32.7365728900256, Blast_Score=180, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6325305, Length=629, Percent_Identity=22.5755166931638, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322597, Length=151, Percent_Identity=31.1258278145695, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17137192, Length=606, Percent_Identity=29.3729372937294, Blast_Score=232, Evalue=5e-61,
Organism=Drosophila melanogaster, GI24582192, Length=516, Percent_Identity=28.6821705426357, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24660907, Length=597, Percent_Identity=28.4757118927973, Blast_Score=187, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24660903, Length=597, Percent_Identity=28.4757118927973, Blast_Score=187, Evalue=3e-47,
Organism=Drosophila melanogaster, GI78706872, Length=531, Percent_Identity=28.6252354048964, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24583543, Length=531, Percent_Identity=28.6252354048964, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI78706876, Length=531, Percent_Identity=28.6252354048964, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24647438, Length=234, Percent_Identity=28.6324786324786, Blast_Score=72, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 66176; Mature: 66176

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKELSSLASPLSQPQVEKLKQLTAELNAVQLAWVSGYLAATANASGNLAQLAPVSDAP
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEEHHCEEEEECCCCCCEEEECCCCCCC
AAQTVTILYGSQTGNGRGIAKALAEKAKAQGYSVNLASMGEYNVRQLKQETLLLLVVSTH
CCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCEEEEEEEECC
GEGEAPDDAIELHKFLASKRAPQLNNLHYSVLALGDSSYEFFCQTGKDFDARLSALGAKA
CCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHH
LLPLVECDVDYEAAAGQWHADVLTAVKPLIQTTANVVALNDTGSAQVASESEFTKQNPYS
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCEECCCCHHCCCCCCC
AEVLVSQKITGRGSDRDVRHVEIDLGESGLRYEVGDALGVWFSNNETLVDEILAGLGLAA
EEEEEEEECCCCCCCCCCEEEEEEECCCCCEEECCCEEEEEECCCCHHHHHHHHHCCCCC
DATVTVGNESINLKQALIEKKELTQLYPGLVKAWAELSASPELLAISEDKEQVRQFILHH
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
QFADLVANYQLKADANLDANKLVELLRPLTPRLYSIASSQSEVDTEVHLTVALVEDEHQG
HHHHHHHCCEEEECCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEEEEEECCCCC
QARFGGASHFLASAEEGAEVKVYVEPNKHFRLPEDPQTPVIMIGPGTGVAPFRAFMQERV
CCCCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
AQGAEGDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLTRIDVAFSRDQAHKIYVQHRIKEQ
HCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHH
GQTLWQWLQNGAHLYICGDAERMAKDVHQALLAVAVEFGGLSSEAAEEYFETLRSHKRYQ
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KDVY
HCCC
>Mature Secondary Structure
MLLKELSSLASPLSQPQVEKLKQLTAELNAVQLAWVSGYLAATANASGNLAQLAPVSDAP
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEEHHCEEEEECCCCCCEEEECCCCCCC
AAQTVTILYGSQTGNGRGIAKALAEKAKAQGYSVNLASMGEYNVRQLKQETLLLLVVSTH
CCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCEEEEEEEECC
GEGEAPDDAIELHKFLASKRAPQLNNLHYSVLALGDSSYEFFCQTGKDFDARLSALGAKA
CCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHH
LLPLVECDVDYEAAAGQWHADVLTAVKPLIQTTANVVALNDTGSAQVASESEFTKQNPYS
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCEECCCCHHCCCCCCC
AEVLVSQKITGRGSDRDVRHVEIDLGESGLRYEVGDALGVWFSNNETLVDEILAGLGLAA
EEEEEEEECCCCCCCCCCEEEEEEECCCCCEEECCCEEEEEECCCCHHHHHHHHHCCCCC
DATVTVGNESINLKQALIEKKELTQLYPGLVKAWAELSASPELLAISEDKEQVRQFILHH
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
QFADLVANYQLKADANLDANKLVELLRPLTPRLYSIASSQSEVDTEVHLTVALVEDEHQG
HHHHHHHCCEEEECCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEEEEEECCCCC
QARFGGASHFLASAEEGAEVKVYVEPNKHFRLPEDPQTPVIMIGPGTGVAPFRAFMQERV
CCCCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
AQGAEGDSWLFFGNPHFEQDFLYQTEWQQYLKNGDLTRIDVAFSRDQAHKIYVQHRIKEQ
HCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHH
GQTLWQWLQNGAHLYICGDAERMAKDVHQALLAVAVEFGGLSSEAAEEYFETLRSHKRYQ
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KDVY
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]