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The map label for this gene is pntA [H]

Identifier: 117919306

GI number: 117919306

Start: 991421

End: 992947

Strand: Reverse

Name: pntA [H]

Synonym: Shewana3_0854

Alternate gene names: 117919306

Gene position: 992947-991421 (Counterclockwise)

Preceding gene: 117919312

Following gene: 117919305

Centisome position: 19.97

GC content: 53.05

Gene sequence:

>1527_bases
ATGCAGATTGGAATACCGAGAGAGAGTCTCGCCGGTGAGACTCGGGTCGCCGCGACCCCTGCCACCGTCGAACAACTTAA
AAAGCTCGGCTTTGAAGTTGTTGTTGAGGCCAATGCTGGCTTACTGTCTAGCTTTGACGATGCCGCTTTTGAAGCCGCTG
GGGCGAAGATCACGGCAGAGGTTTGGCAAGCGGATCTGATTTTTAAGGTCAATGCACCGACCGATGCTGAAATTGCGCAA
ATTAAAGAAGGCGCGACCTTAATCAGCTTTATCTGGCCGGCTCAAAATGCTGAGTTGGTCGAGAAACTGTCTAAACGCAA
CATCAATGTGATGGCGATGGACATGGTGCCACGTATTTCCCGCGCCCAATCCCTCGATGCTCTCTCTTCTATGGCCAATA
TCGGCGGTTATCGCGCCGTTGTTGAAGCCGCGCACCACTTTGGCCGTTTCTTTACCGGACAAATTACTGCCGCGGGTAAG
GTTCCGCCAGCCAAAGTGCTGGTGATTGGTGCCGGTGTAGCGGGTCTTGCCGCCATTGGTACCGCAGGTTCGCTAGGGGC
TATCGTTCGTGCCTTCGACACACGCTTAGAAGTGGCCGAACAAATCGAATCTATGGGTGGCCAGTTCCTCAAACTGGACT
TTACCAATGAAGATGGCAGCTCATCGGACGGTTACGCTAAAACCATGTCGGACGAGTTTATCGCCGCCGAAATGGCACTG
TTTGCCGAGCAAGCCAAAGAAGTCGATATCATCATTACCACAGCGCTGATCCCTGGCCGCCCTGCACCTAAGCTCATTAC
TAAGGCGATGGTCGACAGCATGAAACCCGGCTCTGTGATTGTCGATATGGCCGCGCAAGCGGGCGGTAACTGTGAATACA
CTCAGCCTAGCGAGCTATTCGTGACCGAGAATGGCGTTAAAGTCATCGGCTTTACCGATCTTCCCGGCCGCCTGCCCGCG
CAGTCTTCACAGCTTTACGGCACTAACTTAGTCAACCTGATGAAGTTAATGTGTAAAGAGAAAAACGGCGTCGCCAGCAT
CAACTTCGATGATGTGGTGATGCGCAATATGACAGTGGTCAAAGCAGGCGAAGTGACCTTCCCGCCACCGGCGATTTCGG
TGTCGGCAGCGCCCGCCAAACCTGCGGCTAAGGTTGAAGCCAAAGCGGCTGAAGCCAAAAAGCCATCCAAACTTAAGTAT
ATTCTCGCTGCCCTTGGTGCTGCGGGTTTTGCCGCGGTTGCCTCAGTTGCGCCAGCCGAGTTCTTATCCCACTTTACGGT
TTTTGTCCTGTCCTGCGTGGTGGGCTATTACGTGGTGTGGAACGTATCCCACTCACTACATACGCCTTTAATGTCAGTGA
CTAACGCGATTTCGGGCATTATCGTGGTCGGCGCCTTGCTGCAAATTGGTCAAGGTTCAACCTTGGTCACTGTGCTGGCG
TTTATTGCCGTGCTGATCGCCAGTATTAACATCTTCGGCGGTTTCACCGTCACTCAGCGCATGCTGAAGATGTTCCGTAA
AGATTAA

Upstream 100 bases:

>100_bases
GAATAAACGAGTTCATTCTTATCGCGGCCTAGCCCTACCTAGGTTTAGCTCAGGGGAAGCACTCAGGCGGAACGATTATA
TAAACCACCAGGGAGTAATC

Downstream 100 bases:

>100_bases
GGGGGTATAACGTGTCTCAAGGACTGGTAACTGCAGCTTACATTATTGCCGCCGTGCTCTTTATCTTCAGTCTCGCAGGA
TTGTCGAAGCAAGAAACGGC

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MQIGIPRESLAGETRVAATPATVEQLKKLGFEVVVEANAGLLSSFDDAAFEAAGAKITAEVWQADLIFKVNAPTDAEIAQ
IKEGATLISFIWPAQNAELVEKLSKRNINVMAMDMVPRISRAQSLDALSSMANIGGYRAVVEAAHHFGRFFTGQITAAGK
VPPAKVLVIGAGVAGLAAIGTAGSLGAIVRAFDTRLEVAEQIESMGGQFLKLDFTNEDGSSSDGYAKTMSDEFIAAEMAL
FAEQAKEVDIIITTALIPGRPAPKLITKAMVDSMKPGSVIVDMAAQAGGNCEYTQPSELFVTENGVKVIGFTDLPGRLPA
QSSQLYGTNLVNLMKLMCKEKNGVASINFDDVVMRNMTVVKAGEVTFPPPAISVSAAPAKPAAKVEAKAAEAKKPSKLKY
ILAALGAAGFAAVASVAPAEFLSHFTVFVLSCVVGYYVVWNVSHSLHTPLMSVTNAISGIIVVGALLQIGQGSTLVTVLA
FIAVLIASINIFGGFTVTQRMLKMFRKD

Sequences:

>Translated_508_residues
MQIGIPRESLAGETRVAATPATVEQLKKLGFEVVVEANAGLLSSFDDAAFEAAGAKITAEVWQADLIFKVNAPTDAEIAQ
IKEGATLISFIWPAQNAELVEKLSKRNINVMAMDMVPRISRAQSLDALSSMANIGGYRAVVEAAHHFGRFFTGQITAAGK
VPPAKVLVIGAGVAGLAAIGTAGSLGAIVRAFDTRLEVAEQIESMGGQFLKLDFTNEDGSSSDGYAKTMSDEFIAAEMAL
FAEQAKEVDIIITTALIPGRPAPKLITKAMVDSMKPGSVIVDMAAQAGGNCEYTQPSELFVTENGVKVIGFTDLPGRLPA
QSSQLYGTNLVNLMKLMCKEKNGVASINFDDVVMRNMTVVKAGEVTFPPPAISVSAAPAKPAAKVEAKAAEAKKPSKLKY
ILAALGAAGFAAVASVAPAEFLSHFTVFVLSCVVGYYVVWNVSHSLHTPLMSVTNAISGIIVVGALLQIGQGSTLVTVLA
FIAVLIASINIFGGFTVTQRMLKMFRKD
>Mature_508_residues
MQIGIPRESLAGETRVAATPATVEQLKKLGFEVVVEANAGLLSSFDDAAFEAAGAKITAEVWQADLIFKVNAPTDAEIAQ
IKEGATLISFIWPAQNAELVEKLSKRNINVMAMDMVPRISRAQSLDALSSMANIGGYRAVVEAAHHFGRFFTGQITAAGK
VPPAKVLVIGAGVAGLAAIGTAGSLGAIVRAFDTRLEVAEQIESMGGQFLKLDFTNEDGSSSDGYAKTMSDEFIAAEMAL
FAEQAKEVDIIITTALIPGRPAPKLITKAMVDSMKPGSVIVDMAAQAGGNCEYTQPSELFVTENGVKVIGFTDLPGRLPA
QSSQLYGTNLVNLMKLMCKEKNGVASINFDDVVMRNMTVVKAGEVTFPPPAISVSAAPAKPAAKVEAKAAEAKKPSKLKY
ILAALGAAGFAAVASVAPAEFLSHFTVFVLSCVVGYYVVWNVSHSLHTPLMSVTNAISGIIVVGALLQIGQGSTLVTVLA
FIAVLIASINIFGGFTVTQRMLKMFRKD

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=533, Percent_Identity=49.53095684803, Blast_Score=464, Evalue=1e-131,
Organism=Homo sapiens, GI122939153, Length=533, Percent_Identity=49.53095684803, Blast_Score=464, Evalue=1e-131,
Organism=Escherichia coli, GI1787887, Length=512, Percent_Identity=73.6328125, Blast_Score=743, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=520, Percent_Identity=50.5769230769231, Blast_Score=480, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 53433; Mature: 53433

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGIPRESLAGETRVAATPATVEQLKKLGFEVVVEANAGLLSSFDDAAFEAAGAKITAE
CCCCCCHHHHCCCCEEECCHHHHHHHHHCCEEEEEECCCCCHHCCCHHHHHHCCCEEEHH
VWQADLIFKVNAPTDAEIAQIKEGATLISFIWPAQNAELVEKLSKRNINVMAMDMVPRIS
HCCCEEEEEECCCCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCHHHH
RAQSLDALSSMANIGGYRAVVEAAHHFGRFFTGQITAAGKVPPAKVLVIGAGVAGLAAIG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEECEEEECCCCCCCEEEEEECCHHHHHHHC
TAGSLGAIVRAFDTRLEVAEQIESMGGQFLKLDFTNEDGSSSDGYAKTMSDEFIAAEMAL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHCHHHHHHHHHH
FAEQAKEVDIIITTALIPGRPAPKLITKAMVDSMKPGSVIVDMAAQAGGNCEYTQPSELF
HHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCCCEE
VTENGVKVIGFTDLPGRLPAQSSQLYGTNLVNLMKLMCKEKNGVASINFDDVVMRNMTVV
EECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCHHHHHHHCEEEE
KAGEVTFPPPAISVSAAPAKPAAKVEAKAAEAKKPSKLKYILAALGAAGFAAVASVAPAE
EECCEECCCCCCEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLSHFTVFVLSCVVGYYVVWNVSHSLHTPLMSVTNAISGIIVVGALLQIGQGSTLVTVLA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FIAVLIASINIFGGFTVTQRMLKMFRKD
HHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQIGIPRESLAGETRVAATPATVEQLKKLGFEVVVEANAGLLSSFDDAAFEAAGAKITAE
CCCCCCHHHHCCCCEEECCHHHHHHHHHCCEEEEEECCCCCHHCCCHHHHHHCCCEEEHH
VWQADLIFKVNAPTDAEIAQIKEGATLISFIWPAQNAELVEKLSKRNINVMAMDMVPRIS
HCCCEEEEEECCCCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCHHHH
RAQSLDALSSMANIGGYRAVVEAAHHFGRFFTGQITAAGKVPPAKVLVIGAGVAGLAAIG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEECEEEECCCCCCCEEEEEECCHHHHHHHC
TAGSLGAIVRAFDTRLEVAEQIESMGGQFLKLDFTNEDGSSSDGYAKTMSDEFIAAEMAL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHCHHHHHHHHHH
FAEQAKEVDIIITTALIPGRPAPKLITKAMVDSMKPGSVIVDMAAQAGGNCEYTQPSELF
HHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCCCEE
VTENGVKVIGFTDLPGRLPAQSSQLYGTNLVNLMKLMCKEKNGVASINFDDVVMRNMTVV
EECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCHHHHHHHCEEEE
KAGEVTFPPPAISVSAAPAKPAAKVEAKAAEAKKPSKLKYILAALGAAGFAAVASVAPAE
EECCEECCCCCCEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLSHFTVFVLSCVVGYYVVWNVSHSLHTPLMSVTNAISGIIVVGALLQIGQGSTLVTVLA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FIAVLIASINIFGGFTVTQRMLKMFRKD
HHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]