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The map label for this gene is rlmB [H]

Identifier: 117919150

GI number: 117919150

Start: 814902

End: 815642

Strand: Direct

Name: rlmB [H]

Synonym: Shewana3_0698

Alternate gene names: 117919150

Gene position: 814902-815642 (Clockwise)

Preceding gene: 117919149

Following gene: 117919153

Centisome position: 16.39

GC content: 50.88

Gene sequence:

>741_bases
ATGAAAAAACAAGATATTATTTTTGGGATCCACGCGGTTGAAGCGCTGTTAAAACACAGCCCTGAGCGCATTATTGAACT
TTGGCTGCTGCAGGGCCGTGAAGATGAGCGTTTAACGCCGCTGATCCGCCAGGCTAAAGCCTTTGGCACTAGCATCCAAG
TGACCTCACGCAAAGTATTGGACGATAAAGCCGAAAGTGCTCAGCACCAAGGCATTGTTGCCCGTGTTAAGGCGGCTAAA
ATCCTTGGTGAGCATGACTTAGATGAGTTGTTAGGTAAGACCGATTTACCATTTTTATTGATTTTAGATGGTGTTACCGA
TCCGCATAACCTAGGTGCTTGCTTACGTAACGCCGATGCTGCGGGTGTGCAGGGCATTATTGTGCCTAAGGACAACTCAG
TAGGCTTAACGGCTGTGGTGAGTAAAGTGGCCTGCGGCGCCGCCGAAACTGTGCCCTTATTCCAAGTGACTAACCTTGCG
CGTACCATGCGTCATCTGCAGGAAAAGGGCGTGTGGATTGTTGGCACGGCAGGCGAAGCGGATTGTGAGTTATATCAAGC
CGATCTTAAAGGGCCGCTGGCGATTGCGATGGGCGCCGAAGGCAAAGGCTTACGCCGCTTAAGTCGTGAATGTTGTGATA
CCTTGGTTTCGATTCCGATGGCGGGTAGCGTTTCTAGCTTAAACGTGTCGGTCGCAACGGGTATCTGCCTATTCGAAGCC
GTGCGTCAACGCCGTAGCTAA

Upstream 100 bases:

>100_bases
GAGTAAGTCCAAGTCTAAAGCAGGCGCTAAACCTAAGAAAAGCGTAAAAAATTCGGCGAAAAAGCCAAAGGCCGCGAAGC
GGAGCACGAGAAAGAAGTAA

Downstream 100 bases:

>100_bases
TTGATAGCTTTCAATAAAAAAGACGGGCTAGCCCGTCTTTTTTATTGTCTCGAATAACTGAAATTCCCCTTAAGCGGTTT
TCACCGTATCGAGATCTGAC

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKKQDIIFGIHAVEALLKHSPERIIELWLLQGREDERLTPLIRQAKAFGTSIQVTSRKVLDDKAESAQHQGIVARVKAAK
ILGEHDLDELLGKTDLPFLLILDGVTDPHNLGACLRNADAAGVQGIIVPKDNSVGLTAVVSKVACGAAETVPLFQVTNLA
RTMRHLQEKGVWIVGTAGEADCELYQADLKGPLAIAMGAEGKGLRRLSRECCDTLVSIPMAGSVSSLNVSVATGICLFEA
VRQRRS

Sequences:

>Translated_246_residues
MKKQDIIFGIHAVEALLKHSPERIIELWLLQGREDERLTPLIRQAKAFGTSIQVTSRKVLDDKAESAQHQGIVARVKAAK
ILGEHDLDELLGKTDLPFLLILDGVTDPHNLGACLRNADAAGVQGIIVPKDNSVGLTAVVSKVACGAAETVPLFQVTNLA
RTMRHLQEKGVWIVGTAGEADCELYQADLKGPLAIAMGAEGKGLRRLSRECCDTLVSIPMAGSVSSLNVSVATGICLFEA
VRQRRS
>Mature_246_residues
MKKQDIIFGIHAVEALLKHSPERIIELWLLQGREDERLTPLIRQAKAFGTSIQVTSRKVLDDKAESAQHQGIVARVKAAK
ILGEHDLDELLGKTDLPFLLILDGVTDPHNLGACLRNADAAGVQGIIVPKDNSVGLTAVVSKVACGAAETVPLFQVTNLA
RTMRHLQEKGVWIVGTAGEADCELYQADLKGPLAIAMGAEGKGLRRLSRECCDTLVSIPMAGSVSSLNVSVATGICLFEA
VRQRRS

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=263, Percent_Identity=29.277566539924, Blast_Score=92, Evalue=3e-19,
Organism=Escherichia coli, GI1790623, Length=240, Percent_Identity=66.25, Blast_Score=331, Evalue=2e-92,
Organism=Escherichia coli, GI1788935, Length=244, Percent_Identity=27.0491803278689, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790083, Length=162, Percent_Identity=30.8641975308642, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17555908, Length=258, Percent_Identity=27.906976744186, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324775, Length=172, Percent_Identity=36.046511627907, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26448; Mature: 26448

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQDIIFGIHAVEALLKHSPERIIELWLLQGREDERLTPLIRQAKAFGTSIQVTSRKVL
CCCCCCEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEHHHHHH
DDKAESAQHQGIVARVKAAKILGEHDLDELLGKTDLPFLLILDGVTDPHNLGACLRNADA
HHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCC
AGVQGIIVPKDNSVGLTAVVSKVACGAAETVPLFQVTNLARTMRHLQEKGVWIVGTAGEA
CCCEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCC
DCELYQADLKGPLAIAMGAEGKGLRRLSRECCDTLVSIPMAGSVSSLNVSVATGICLFEA
CHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
VRQRRS
HHHHCC
>Mature Secondary Structure
MKKQDIIFGIHAVEALLKHSPERIIELWLLQGREDERLTPLIRQAKAFGTSIQVTSRKVL
CCCCCCEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEHHHHHH
DDKAESAQHQGIVARVKAAKILGEHDLDELLGKTDLPFLLILDGVTDPHNLGACLRNADA
HHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCC
AGVQGIIVPKDNSVGLTAVVSKVACGAAETVPLFQVTNLARTMRHLQEKGVWIVGTAGEA
CCCEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCC
DCELYQADLKGPLAIAMGAEGKGLRRLSRECCDTLVSIPMAGSVSSLNVSVATGICLFEA
CHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
VRQRRS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]