Click here to switch to the map view.

The map label for this gene is amiB [H]

Identifier: 117919047

GI number: 117919047

Start: 696509

End: 697900

Strand: Direct

Name: amiB [H]

Synonym: Shewana3_0594

Alternate gene names: 117919047

Gene position: 696509-697900 (Clockwise)

Preceding gene: 117919046

Following gene: 117919048

Centisome position: 14.01

GC content: 48.49

Gene sequence:

>1392_bases
ATGACTAAAAATAAGCATTACTTTCAGACTATTACTCTTTTTCTGTGCGCTTTTTTTGCTGTAGCAGCTCATGCTGCTAA
CCAGCTTGAGGGCGTGCGTATTTGGGCTGCCCCCGAGTCGACCCGTGTGGTGTTCGATTTGAGTGAAGCGCCAAACTACA
CTTATTTTTCCCTCGATGGGCCCAATCGTTTAGTGGTCGACCTTAAAAAGGCGTCGACAAAACTGAGCCTGAAAAATATC
GATAACAACAGTAAATTGGTTAAAGGGGTTCGCGTCAGCAAATCACCCACTAAAGGTGATTTACGTTTGGTGATCGATCT
TGTTAAGCCATTAAATGCTAACTTGTTTTCCCTTCCCGAGACGGCGCCCTATGGTAATCGTTTGGTGGTGGATCTTGAGG
ATAAAACCTTAACGACGGCAACAGCTGTGGTGAGTTCGACTCCCGTTAAAACGGTTACTCAATCGGCGCAATCCTCCCGT
GATATTGTGGTAGCGATTGATGCGGGCCATGGTGGTGACGATCCTGGTTCTATTGGCCCTTCAGGTGTTTACGAGAAAAA
AGTGGCACTGGAGATAGCAAAAAGAGTGGCCAGTAAGATCAACGACACGCCGGGTATGCGGGCAGTGATGATCCGTACCG
GCGATTACTTTGTGAATTTGAACAAGCGCTCCGAGCTCGCCCGTAACAGTAAGGCCGATTTACTGATTTCTATCCACGCC
GATGCTTTTACCTCGCCTCAGCCAAAAGGAGCGTCTGTGTGGGTATTGTCGATGCGCCGCGCGAACAGTGAAATTGGTCG
TTGGTTAGAGCAAAAAGAGAAACACTCAGAACTCTTAGGTGGTGCAGGGGAAATTATTCAAAATACCGATAATGAACAGT
ATCTTGCAATGACTCTGCTCGACATGTCCATGAACAGTTCGATGGCTATTGGTCACTCAGTTGCTGGGGATATTCTCAAG
GATCTCGGTTCAGTGACTGAGCTGCATAAGAGTCGCCCTGAGTCTGCGAGCCTTGCGGTATTAAAATCCCCCGATATTCC
CTCTATTCTGGTGGAGACAGGATTTATTTCGAATCCGAAGGAAGAAAGGTTACTTAATAGCAGTCGCCATCAGGAAAGTA
TCGCAACCGCTATCTATAAGGGCGTTAGCCGTTATTTCCACAATAACCCGCCGGCAGATACCTTGCTCGCGAAGCGTAAT
AGCGGCGGCACAACGGCCAGCTCGAGCGCGAAATCCTCCTCGGGAAATATCAAGCACAAGGTGAGCCGCGGCGAGTCACT
TTCGGCGATTGCTCAGCGTTATCAAGTGCCTATGGCTAGCATTAAGCGCGCCAATGGCATGAAGACGGATGTGGTACAAC
TCGGGCAAACACTCGTTATTCCTGAGAGTTAA

Upstream 100 bases:

>100_bases
ACCTGCATTTAAATTATGTTAACCAAGGACGTGAAATCCAAATTCGAGCCTTAACAGAATCAGGTAAAAAGCTATTAGCC
GCAATAAAATAAAAACAAAG

Downstream 100 bases:

>100_bases
TAGGATGAAGTCTATGGGGATCCAGATCCTTCCGCCGCAACTAGCCAACCAAATCGCCGCTGGTGAAGTGGTCGAACGGC
CCGCTTCCGTGGTGAAGGAA

Product: cell wall hydrolase/autolysin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTKNKHYFQTITLFLCAFFAVAAHAANQLEGVRIWAAPESTRVVFDLSEAPNYTYFSLDGPNRLVVDLKKASTKLSLKNI
DNNSKLVKGVRVSKSPTKGDLRLVIDLVKPLNANLFSLPETAPYGNRLVVDLEDKTLTTATAVVSSTPVKTVTQSAQSSR
DIVVAIDAGHGGDDPGSIGPSGVYEKKVALEIAKRVASKINDTPGMRAVMIRTGDYFVNLNKRSELARNSKADLLISIHA
DAFTSPQPKGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMNSSMAIGHSVAGDILK
DLGSVTELHKSRPESASLAVLKSPDIPSILVETGFISNPKEERLLNSSRHQESIATAIYKGVSRYFHNNPPADTLLAKRN
SGGTTASSSAKSSSGNIKHKVSRGESLSAIAQRYQVPMASIKRANGMKTDVVQLGQTLVIPES

Sequences:

>Translated_463_residues
MTKNKHYFQTITLFLCAFFAVAAHAANQLEGVRIWAAPESTRVVFDLSEAPNYTYFSLDGPNRLVVDLKKASTKLSLKNI
DNNSKLVKGVRVSKSPTKGDLRLVIDLVKPLNANLFSLPETAPYGNRLVVDLEDKTLTTATAVVSSTPVKTVTQSAQSSR
DIVVAIDAGHGGDDPGSIGPSGVYEKKVALEIAKRVASKINDTPGMRAVMIRTGDYFVNLNKRSELARNSKADLLISIHA
DAFTSPQPKGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMNSSMAIGHSVAGDILK
DLGSVTELHKSRPESASLAVLKSPDIPSILVETGFISNPKEERLLNSSRHQESIATAIYKGVSRYFHNNPPADTLLAKRN
SGGTTASSSAKSSSGNIKHKVSRGESLSAIAQRYQVPMASIKRANGMKTDVVQLGQTLVIPES
>Mature_462_residues
TKNKHYFQTITLFLCAFFAVAAHAANQLEGVRIWAAPESTRVVFDLSEAPNYTYFSLDGPNRLVVDLKKASTKLSLKNID
NNSKLVKGVRVSKSPTKGDLRLVIDLVKPLNANLFSLPETAPYGNRLVVDLEDKTLTTATAVVSSTPVKTVTQSAQSSRD
IVVAIDAGHGGDDPGSIGPSGVYEKKVALEIAKRVASKINDTPGMRAVMIRTGDYFVNLNKRSELARNSKADLLISIHAD
AFTSPQPKGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMNSSMAIGHSVAGDILKD
LGSVTELHKSRPESASLAVLKSPDIPSILVETGFISNPKEERLLNSSRHQESIATAIYKGVSRYFHNNPPADTLLAKRNS
GGTTASSSAKSSSGNIKHKVSRGESLSAIAQRYQVPMASIKRANGMKTDVVQLGQTLVIPES

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=425, Percent_Identity=37.1764705882353, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI87082163, Length=396, Percent_Identity=34.8484848484849, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1788776, Length=225, Percent_Identity=43.1111111111111, Blast_Score=180, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 50042; Mature: 49910

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNKHYFQTITLFLCAFFAVAAHAANQLEGVRIWAAPESTRVVFDLSEAPNYTYFSLDG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCEEEEEECC
PNRLVVDLKKASTKLSLKNIDNNSKLVKGVRVSKSPTKGDLRLVIDLVKPLNANLFSLPE
CCEEEEEEECCCCEEEEECCCCCCHHEECEEECCCCCCCHHHHHEEHHCCCCCCEEECCC
TAPYGNRLVVDLEDKTLTTATAVVSSTPVKTVTQSAQSSRDIVVAIDAGHGGDDPGSIGP
CCCCCCEEEEEECCCCEEHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
SGVYEKKVALEIAKRVASKINDTPGMRAVMIRTGDYFVNLNKRSELARNSKADLLISIHA
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEECCHHHHHCCCCCCEEEEEEC
DAFTSPQPKGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLL
CCCCCCCCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEE
DMSMNSSMAIGHSVAGDILKDLGSVTELHKSRPESASLAVLKSPDIPSILVETGFISNPK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCH
EERLLNSSRHQESIATAIYKGVSRYFHNNPPADTLLAKRNSGGTTASSSAKSSSGNIKHK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCCCCCCCCCCEEEE
VSRGESLSAIAQRYQVPMASIKRANGMKTDVVQLGQTLVIPES
CCCCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHCCCEEECCCC
>Mature Secondary Structure 
TKNKHYFQTITLFLCAFFAVAAHAANQLEGVRIWAAPESTRVVFDLSEAPNYTYFSLDG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCEEEEEECC
PNRLVVDLKKASTKLSLKNIDNNSKLVKGVRVSKSPTKGDLRLVIDLVKPLNANLFSLPE
CCEEEEEEECCCCEEEEECCCCCCHHEECEEECCCCCCCHHHHHEEHHCCCCCCEEECCC
TAPYGNRLVVDLEDKTLTTATAVVSSTPVKTVTQSAQSSRDIVVAIDAGHGGDDPGSIGP
CCCCCCEEEEEECCCCEEHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
SGVYEKKVALEIAKRVASKINDTPGMRAVMIRTGDYFVNLNKRSELARNSKADLLISIHA
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEECCHHHHHCCCCCCEEEEEEC
DAFTSPQPKGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLL
CCCCCCCCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEE
DMSMNSSMAIGHSVAGDILKDLGSVTELHKSRPESASLAVLKSPDIPSILVETGFISNPK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCH
EERLLNSSRHQESIATAIYKGVSRYFHNNPPADTLLAKRNSGGTTASSSAKSSSGNIKHK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCCCCCCCCCCEEEE
VSRGESLSAIAQRYQVPMASIKRANGMKTDVVQLGQTLVIPES
CCCCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 2676972 [H]