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The map label for this gene is rimK1 [H]

Identifier: 117918999

GI number: 117918999

Start: 640850

End: 641755

Strand: Direct

Name: rimK1 [H]

Synonym: Shewana3_0545

Alternate gene names: 117918999

Gene position: 640850-641755 (Clockwise)

Preceding gene: 117918998

Following gene: 117919003

Centisome position: 12.89

GC content: 51.21

Gene sequence:

>906_bases
ATGAAAATAGGTATCTTATCCCAGTTCCCTGAGTTGTATTCCACCCGACGTTTAGTGGCGGCCTGCGAGAGCCGTGGTCA
TGAGGCTGTGGTTATTAATACCTTGAATTGCTACATGAATATCAACTCCATCAAGCCGAGCATTCATTACCAAGGCCAAG
AATTAACGGGGTTCGATGCCATTATCCCGCGCATTCACGCCAGCGTGACCTTTTATGGCTGCGCGGTAGTGCGCCAGTTC
GAAATGATGGGGGTATTTGCCGCCAATGACTCTATTTCAATCGCGCGTTCACGGGACAAGTTACGCGCGCTGCAATTGCT
TTCCCGCAAAGGCATAGGCATGCCTATTACTGGATTTGCCAATAAACCCAATGATATTCCCGATTTGATTAACATGGTGG
GCGGCGCGCCCTTAGTGATTAAGTTGCTGGAAGGCACACAAGGAATTGGCGTGGTGTTGGCCGAGACCAAAACCGCTGCC
GAGAGCGTGATCGAAGCCTTTTTAGGGCTGAAAGCCAATATCATGGTGCAGGAATATATTAAGGAATCCAACGGTAGCGA
CATTCGCTGTTTTGTGGTCGGCGATAAAGTGGTGGCTTCGATGAAGCGCCAAGGACCCGAAGGAGATTTTCGCTCTAACC
TGCACTTAGGTGGCTGTGGTGAGAAAGTGAAAATCACCCCTGCGGAGCGCAAGATGGCGGTTGCCGCAGTGAAAGCCATG
GGCTTAGTGGTGGCTGGGGTCGATATTTTACGCTCGAATCGCGGTCCGTTGATCCTTGAGGTGAATTCGGCGCCCGGTAT
CGAAGGGATTGAGCAGACCACGGGGATTTCGGTCACTGAACCCATCGTAGAGTACATTGAGAAGATGGTATCGGCGCGTA
AATCGAATCGCGCCGTGATTGCCTAA

Upstream 100 bases:

>100_bases
TTTTCTTGCTTTACACTCAGCAATAGACAAATATCAGCGCCCCTTTCTGATGCAGTGTGCTCAGAGCTTCAATCACAGTG
TTTAATTTGGTCATTTGGCA

Downstream 100 bases:

>100_bases
TAAAAAACGCCGCGATTATCGCGGCGTTTTTGTTTTACTCGCGCTTAATTTAGTGCTTAATCTTATAAATCAAAGCCGTA
GGAATAGCCTAAAACTACCT

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKIGILSQFPELYSTRRLVAACESRGHEAVVINTLNCYMNINSIKPSIHYQGQELTGFDAIIPRIHASVTFYGCAVVRQF
EMMGVFAANDSISIARSRDKLRALQLLSRKGIGMPITGFANKPNDIPDLINMVGGAPLVIKLLEGTQGIGVVLAETKTAA
ESVIEAFLGLKANIMVQEYIKESNGSDIRCFVVGDKVVASMKRQGPEGDFRSNLHLGGCGEKVKITPAERKMAVAAVKAM
GLVVAGVDILRSNRGPLILEVNSAPGIEGIEQTTGISVTEPIVEYIEKMVSARKSNRAVIA

Sequences:

>Translated_301_residues
MKIGILSQFPELYSTRRLVAACESRGHEAVVINTLNCYMNINSIKPSIHYQGQELTGFDAIIPRIHASVTFYGCAVVRQF
EMMGVFAANDSISIARSRDKLRALQLLSRKGIGMPITGFANKPNDIPDLINMVGGAPLVIKLLEGTQGIGVVLAETKTAA
ESVIEAFLGLKANIMVQEYIKESNGSDIRCFVVGDKVVASMKRQGPEGDFRSNLHLGGCGEKVKITPAERKMAVAAVKAM
GLVVAGVDILRSNRGPLILEVNSAPGIEGIEQTTGISVTEPIVEYIEKMVSARKSNRAVIA
>Mature_301_residues
MKIGILSQFPELYSTRRLVAACESRGHEAVVINTLNCYMNINSIKPSIHYQGQELTGFDAIIPRIHASVTFYGCAVVRQF
EMMGVFAANDSISIARSRDKLRALQLLSRKGIGMPITGFANKPNDIPDLINMVGGAPLVIKLLEGTQGIGVVLAETKTAA
ESVIEAFLGLKANIMVQEYIKESNGSDIRCFVVGDKVVASMKRQGPEGDFRSNLHLGGCGEKVKITPAERKMAVAAVKAM
GLVVAGVDILRSNRGPLILEVNSAPGIEGIEQTTGISVTEPIVEYIEKMVSARKSNRAVIA

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=229, Percent_Identity=28.3842794759825, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI134254462, Length=304, Percent_Identity=24.6710526315789, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1787076, Length=291, Percent_Identity=59.7938144329897, Blast_Score=375, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 32400; Mature: 32400

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGILSQFPELYSTRRLVAACESRGHEAVVINTLNCYMNINSIKPSIHYQGQELTGFDA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCCEEECCCCCCCHHH
IIPRIHASVTFYGCAVVRQFEMMGVFAANDSISIARSRDKLRALQLLSRKGIGMPITGFA
HHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
NKPNDIPDLINMVGGAPLVIKLLEGTQGIGVVLAETKTAAESVIEAFLGLKANIMVQEYI
CCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHH
KESNGSDIRCFVVGDKVVASMKRQGPEGDFRSNLHLGGCGEKVKITPAERKMAVAAVKAM
HCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCEECCCCCEEEECHHHHHHHHHHHHHH
GLVVAGVDILRSNRGPLILEVNSAPGIEGIEQTTGISVTEPIVEYIEKMVSARKSNRAVI
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
A
C
>Mature Secondary Structure
MKIGILSQFPELYSTRRLVAACESRGHEAVVINTLNCYMNINSIKPSIHYQGQELTGFDA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCCEEECCCCCCCHHH
IIPRIHASVTFYGCAVVRQFEMMGVFAANDSISIARSRDKLRALQLLSRKGIGMPITGFA
HHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
NKPNDIPDLINMVGGAPLVIKLLEGTQGIGVVLAETKTAAESVIEAFLGLKANIMVQEYI
CCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHH
KESNGSDIRCFVVGDKVVASMKRQGPEGDFRSNLHLGGCGEKVKITPAERKMAVAAVKAM
HCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCEECCCCCEEEECHHHHHHHHHHHHHH
GLVVAGVDILRSNRGPLILEVNSAPGIEGIEQTTGISVTEPIVEYIEKMVSARKSNRAVI
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA