Click here to switch to the map view.

The map label for this gene is gap3 [H]

Identifier: 117918988

GI number: 117918988

Start: 627881

End: 628894

Strand: Direct

Name: gap3 [H]

Synonym: Shewana3_0534

Alternate gene names: 117918988

Gene position: 627881-628894 (Clockwise)

Preceding gene: 117918987

Following gene: 117918989

Centisome position: 12.63

GC content: 51.48

Gene sequence:

>1014_bases
ATGGCAATTAAAATCGGGATTAATGGATTTGGGCGCATGGGACGTTTGGCTCTACGTGCGGCGTGGGAATGGGATGATGT
TGAGTTTGTGCAGATCAACGATCCCGCTGGGGATGCGGCTACCTTGGCTCATTTGCTTGAGTTCGATTCAGTTCACGGTC
GCTGGCGTTATCCGGTCACGGCGAATCAGGATGGTATTCAAATCCAAGACAAAACCATTCGCACCACACGTAATAAGACG
ATTGCCGAGACCGATTGGTCGGGATGCGATGTGGTGATTGAAGCCTCTGGGGTGATGAAAACCAAAGCCTTACTGCAAGC
TTATTTAGATCAAGGTGTAAAGCGGGTGGTAGTGACGGCGCCAGTCAAAGAGGAAGGTGTGTTAAATGTGGTGATGGGAG
TGAATCATCAGTTATACGATCCGGCGATTCATCCGATTGTGACCGCGGCTTCCTGCACTACCAACTGTCTTGCGCCTGTG
GTGAAAGTGATCCATGAGCAGATTGGCATCAAGCATGGTTCTATGACCACTATCCATGATATTACCAACACCCAGACGAT
TCTGGATGCACCGCACAAGGATCTGCGCCGCGCCCGCGCTTGTGGGTTAAGTTTAATTCCCACCACCACGGGCAGTGCGA
CGGCAATTACCCATATATTCCCTGAGCTTAAGGGTAAGCTGAATGGCCATGCCGTACGAGTGCCGCTCGCTAACGCATCT
CTTACCGACTGTGTTTTTGAGCTTGAGCGCAGCGTGACCGAAGCGGAAGTGAATATGCTGCTACAAACCGCCGCCGAAGG
CGAACTCAAGGGCATTTTAGGTTATGAAGAGCGGCCATTAGTGTCGGTTGATTATAAAACCGATCCCCGTTCGAGCATTG
TCGATGCGCTTTCGACCATGGTGGTGAATGGTACTCAGCTAAAACTCTACGTGTGGTACGACAATGAGTGGGGATATGCC
AACCGTACGGCAGAGCTTGCCCGCTTAGTCGGCCAGCTTGATTTGCCGCGTTAG

Upstream 100 bases:

>100_bases
TTGCCAATCGCACCCGTCATTGGTTTACGACTTAATCTGTTTTTAAAATAAATAATATTAAAAATCAATAAATTGAAAGT
TAACTAGGTAGTTATAAATC

Downstream 100 bases:

>100_bases
GAGAGCACTGTGCCTGCTGCCAAGCTATTTGAGCAACTGATGCAATTGCCCGCTGCCGTGCGGCAATATTTGCTGATCAC
CTTCAATTATTGGAGTTTTA

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MAIKIGINGFGRMGRLALRAAWEWDDVEFVQINDPAGDAATLAHLLEFDSVHGRWRYPVTANQDGIQIQDKTIRTTRNKT
IAETDWSGCDVVIEASGVMKTKALLQAYLDQGVKRVVVTAPVKEEGVLNVVMGVNHQLYDPAIHPIVTAASCTTNCLAPV
VKVIHEQIGIKHGSMTTIHDITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANAS
LTDCVFELERSVTEAEVNMLLQTAAEGELKGILGYEERPLVSVDYKTDPRSSIVDALSTMVVNGTQLKLYVWYDNEWGYA
NRTAELARLVGQLDLPR

Sequences:

>Translated_337_residues
MAIKIGINGFGRMGRLALRAAWEWDDVEFVQINDPAGDAATLAHLLEFDSVHGRWRYPVTANQDGIQIQDKTIRTTRNKT
IAETDWSGCDVVIEASGVMKTKALLQAYLDQGVKRVVVTAPVKEEGVLNVVMGVNHQLYDPAIHPIVTAASCTTNCLAPV
VKVIHEQIGIKHGSMTTIHDITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANAS
LTDCVFELERSVTEAEVNMLLQTAAEGELKGILGYEERPLVSVDYKTDPRSSIVDALSTMVVNGTQLKLYVWYDNEWGYA
NRTAELARLVGQLDLPR
>Mature_336_residues
AIKIGINGFGRMGRLALRAAWEWDDVEFVQINDPAGDAATLAHLLEFDSVHGRWRYPVTANQDGIQIQDKTIRTTRNKTI
AETDWSGCDVVIEASGVMKTKALLQAYLDQGVKRVVVTAPVKEEGVLNVVMGVNHQLYDPAIHPIVTAASCTTNCLAPVV
KVIHEQIGIKHGSMTTIHDITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANASL
TDCVFELERSVTEAEVNMLLQTAAEGELKGILGYEERPLVSVDYKTDPRSSIVDALSTMVVNGTQLKLYVWYDNEWGYAN
RTAELARLVGQLDLPR

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=327, Percent_Identity=41.8960244648318, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI7657116, Length=335, Percent_Identity=39.4029850746269, Blast_Score=246, Evalue=2e-65,
Organism=Escherichia coli, GI1788079, Length=329, Percent_Identity=42.8571428571429, Blast_Score=264, Evalue=6e-72,
Organism=Escherichia coli, GI1789295, Length=328, Percent_Identity=41.7682926829268, Blast_Score=253, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17534677, Length=330, Percent_Identity=41.8181818181818, Blast_Score=267, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI17534679, Length=330, Percent_Identity=41.2121212121212, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17568413, Length=330, Percent_Identity=41.8181818181818, Blast_Score=264, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI32566163, Length=334, Percent_Identity=41.3173652694611, Blast_Score=264, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6322409, Length=333, Percent_Identity=41.4414414414414, Blast_Score=263, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6321631, Length=333, Percent_Identity=41.1411411411411, Blast_Score=261, Evalue=9e-71,
Organism=Saccharomyces cerevisiae, GI6322468, Length=333, Percent_Identity=40.5405405405405, Blast_Score=259, Evalue=4e-70,
Organism=Drosophila melanogaster, GI19922412, Length=336, Percent_Identity=39.2857142857143, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI17933600, Length=334, Percent_Identity=39.5209580838323, Blast_Score=244, Evalue=5e-65,
Organism=Drosophila melanogaster, GI18110149, Length=334, Percent_Identity=39.5209580838323, Blast_Score=244, Evalue=5e-65,
Organism=Drosophila melanogaster, GI85725000, Length=334, Percent_Identity=39.5209580838323, Blast_Score=244, Evalue=9e-65,
Organism=Drosophila melanogaster, GI22023983, Length=334, Percent_Identity=39.5209580838323, Blast_Score=244, Evalue=9e-65,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 36931; Mature: 36800

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKIGINGFGRMGRLALRAAWEWDDVEFVQINDPAGDAATLAHLLEFDSVHGRWRYPVT
CEEEEECCCCCCHHHHHEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEECCEE
ANQDGIQIQDKTIRTTRNKTIAETDWSGCDVVIEASGVMKTKALLQAYLDQGVKRVVVTA
CCCCCCEEECCEEEECCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEC
PVKEEGVLNVVMGVNHQLYDPAIHPIVTAASCTTNCLAPVVKVIHEQIGIKHGSMTTIHD
CCCCCCCEEEEECCCCCCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
ITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANAS
CCCCHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCH
LTDCVFELERSVTEAEVNMLLQTAAEGELKGILGYEERPLVSVDYKTDPRSSIVDALSTM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHHH
VVNGTQLKLYVWYDNEWGYANRTAELARLVGQLDLPR
EECCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AIKIGINGFGRMGRLALRAAWEWDDVEFVQINDPAGDAATLAHLLEFDSVHGRWRYPVT
EEEEECCCCCCHHHHHEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEECCEE
ANQDGIQIQDKTIRTTRNKTIAETDWSGCDVVIEASGVMKTKALLQAYLDQGVKRVVVTA
CCCCCCEEECCEEEECCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEC
PVKEEGVLNVVMGVNHQLYDPAIHPIVTAASCTTNCLAPVVKVIHEQIGIKHGSMTTIHD
CCCCCCCEEEEECCCCCCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
ITNTQTILDAPHKDLRRARACGLSLIPTTTGSATAITHIFPELKGKLNGHAVRVPLANAS
CCCCHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCH
LTDCVFELERSVTEAEVNMLLQTAAEGELKGILGYEERPLVSVDYKTDPRSSIVDALSTM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHHH
VVNGTQLKLYVWYDNEWGYANRTAELARLVGQLDLPR
EECCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8378350 [H]