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The map label for this gene is moaA [H]

Identifier: 116872442

GI number: 116872442

Start: 1040266

End: 1041267

Strand: Direct

Name: moaA [H]

Synonym: lwe1024

Alternate gene names: 116872442

Gene position: 1040266-1041267 (Clockwise)

Preceding gene: 116872441

Following gene: 116872446

Centisome position: 36.97

GC content: 37.52

Gene sequence:

>1002_bases
ATGCAATTATTAAAGGATAAATTTGGGCGGGTACACGATTATATACGCATTTCAGTAACGGATCGCTGTAATTTAAGGTG
TGTGTATTGTATGCCCGAAGAAGGCCTGACATTTTTGCCCCATGAGAAAGTACTATCCAAAGATGAAATTGTCAGTTTTA
TGGAGCTAATGGTACAATTTGGCATTAAAAAAGTACGTATTACTGGTGGAGAACCGCTACTTAGAACCGATATTGTAGAA
ATTGTTCGTGGGCTTGGAGCAATTCCTGAAATAGAAGATATTTCCATTACAACGAATGCGATGTATTTGGCAAAAAAAGC
AGAAGCACTTAAAGAAGCTGGACTTACACGCGTAAATATTAGTTTGGATTCATTACATGCAGATCGTTTTCAAGCAATTA
CACGCGGCGGAAGATTGCAAAAAGTACTTGATGGTATTCAAAAAGCAGAAGAGGTTGGGCTGTTCCCAATTAAACTCAAT
GTTGTTTTAATCAAAGGGCAAAACGATGACGAAATAACTGATTTTCTCAAGTTTACTAAAGATAAAGATATTAATATTCG
CTTTATTGAATATATGCCAATTGGTCACGCAGGTACGAGCTGGAAAGAAAAATATTTGCCGCTAGATACAATTTTTGAAG
CGTGTGATGCGATTGGTTTTGAATATGAAGCAGTTGATTCTATTCGTGGAAATGGTCCTTCTGAAAATTTCCGAATTAAA
GGAGCGAAAGGGACTTTTGGTGTGATTCATCCAGTTAGCTCCCATTTTTGTGACAGCTGTAATCGACTCCGACTCACAGC
AGATGGGTATATCAAAGCATGTCTATATTGGGATGAGGAAATGAACATTCGCCCATTTATACATGAGCCTGTGAAACTCA
TGCAACTCGTGCAAAAAGCAATTGATAATAAACCAGAAAATCATGAAATGGCACTCAAATTACAAGATGAGGTTCAATCT
AATAAGCCAACTTGGCGACGTATGAGTCAAATTGGTGGATAA

Upstream 100 bases:

>100_bases
ATGCGCATTGAAAAAACCTATTTAGTTGAAAAAACAGGTGGGAAAAGTGGTACATTTAAAGCAGAAGCGTAACTATTTTG
TAGGATAGGAGTTTTGTTTT

Downstream 100 bases:

>100_bases
AATAAAACATCCGGCGCAAATTTCGGCCGGATGTTTTATTTTTGTCGTTCTGCTATTAAATGTGGTAATTCAGGTATAAT
CAATTTTTCCATAGCGAGTT

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MQLLKDKFGRVHDYIRISVTDRCNLRCVYCMPEEGLTFLPHEKVLSKDEIVSFMELMVQFGIKKVRITGGEPLLRTDIVE
IVRGLGAIPEIEDISITTNAMYLAKKAEALKEAGLTRVNISLDSLHADRFQAITRGGRLQKVLDGIQKAEEVGLFPIKLN
VVLIKGQNDDEITDFLKFTKDKDINIRFIEYMPIGHAGTSWKEKYLPLDTIFEACDAIGFEYEAVDSIRGNGPSENFRIK
GAKGTFGVIHPVSSHFCDSCNRLRLTADGYIKACLYWDEEMNIRPFIHEPVKLMQLVQKAIDNKPENHEMALKLQDEVQS
NKPTWRRMSQIGG

Sequences:

>Translated_333_residues
MQLLKDKFGRVHDYIRISVTDRCNLRCVYCMPEEGLTFLPHEKVLSKDEIVSFMELMVQFGIKKVRITGGEPLLRTDIVE
IVRGLGAIPEIEDISITTNAMYLAKKAEALKEAGLTRVNISLDSLHADRFQAITRGGRLQKVLDGIQKAEEVGLFPIKLN
VVLIKGQNDDEITDFLKFTKDKDINIRFIEYMPIGHAGTSWKEKYLPLDTIFEACDAIGFEYEAVDSIRGNGPSENFRIK
GAKGTFGVIHPVSSHFCDSCNRLRLTADGYIKACLYWDEEMNIRPFIHEPVKLMQLVQKAIDNKPENHEMALKLQDEVQS
NKPTWRRMSQIGG
>Mature_333_residues
MQLLKDKFGRVHDYIRISVTDRCNLRCVYCMPEEGLTFLPHEKVLSKDEIVSFMELMVQFGIKKVRITGGEPLLRTDIVE
IVRGLGAIPEIEDISITTNAMYLAKKAEALKEAGLTRVNISLDSLHADRFQAITRGGRLQKVLDGIQKAEEVGLFPIKLN
VVLIKGQNDDEITDFLKFTKDKDINIRFIEYMPIGHAGTSWKEKYLPLDTIFEACDAIGFEYEAVDSIRGNGPSENFRIK
GAKGTFGVIHPVSSHFCDSCNRLRLTADGYIKACLYWDEEMNIRPFIHEPVKLMQLVQKAIDNKPENHEMALKLQDEVQS
NKPTWRRMSQIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=336, Percent_Identity=38.0952380952381, Blast_Score=244, Evalue=6e-65,
Organism=Homo sapiens, GI22027536, Length=336, Percent_Identity=38.0952380952381, Blast_Score=244, Evalue=9e-65,
Organism=Escherichia coli, GI1786999, Length=337, Percent_Identity=31.4540059347181, Blast_Score=184, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17561210, Length=310, Percent_Identity=42.5806451612903, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI32566331, Length=334, Percent_Identity=38.9221556886228, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI32566329, Length=309, Percent_Identity=41.1003236245955, Blast_Score=247, Evalue=8e-66,
Organism=Drosophila melanogaster, GI28574488, Length=338, Percent_Identity=41.4201183431953, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI28574490, Length=316, Percent_Identity=42.0886075949367, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 37835; Mature: 37835

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLLKDKFGRVHDYIRISVTDRCNLRCVYCMPEEGLTFLPHEKVLSKDEIVSFMELMVQF
CCHHHHHHHHHEEEEEEEEECCCCEEEEEEECCCCCEECCHHHHCCHHHHHHHHHHHHHH
GIKKVRITGGEPLLRTDIVEIVRGLGAIPEIEDISITTNAMYLAKKAEALKEAGLTRVNI
CCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEE
SLDSLHADRFQAITRGGRLQKVLDGIQKAEEVGLFPIKLNVVLIKGQNDDEITDFLKFTK
EHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCHHHHHHHHHCC
DKDINIRFIEYMPIGHAGTSWKEKYLPLDTIFEACDAIGFEYEAVDSIRGNGPSENFRIK
CCCCEEEEEEEEECCCCCCCCHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEE
GAKGTFGVIHPVSSHFCDSCNRLRLTADGYIKACLYWDEEMNIRPFIHEPVKLMQLVQKA
ECCCCCEEECCHHHHHHCCCCEEEEEECCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
IDNKPENHEMALKLQDEVQSNKPTWRRMSQIGG
HCCCCCCCEEEEEEHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MQLLKDKFGRVHDYIRISVTDRCNLRCVYCMPEEGLTFLPHEKVLSKDEIVSFMELMVQF
CCHHHHHHHHHEEEEEEEEECCCCEEEEEEECCCCCEECCHHHHCCHHHHHHHHHHHHHH
GIKKVRITGGEPLLRTDIVEIVRGLGAIPEIEDISITTNAMYLAKKAEALKEAGLTRVNI
CCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEE
SLDSLHADRFQAITRGGRLQKVLDGIQKAEEVGLFPIKLNVVLIKGQNDDEITDFLKFTK
EHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCHHHHHHHHHCC
DKDINIRFIEYMPIGHAGTSWKEKYLPLDTIFEACDAIGFEYEAVDSIRGNGPSENFRIK
CCCCEEEEEEEEECCCCCCCCHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEE
GAKGTFGVIHPVSSHFCDSCNRLRLTADGYIKACLYWDEEMNIRPFIHEPVKLMQLVQKA
ECCCCCEEECCHHHHHHCCCCEEEEEECCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
IDNKPENHEMALKLQDEVQSNKPTWRRMSQIGG
HCCCCCCCEEEEEEHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]