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The map label for this gene is gbuB [H]

Identifier: 116872417

GI number: 116872417

Start: 1020110

End: 1020958

Strand: Direct

Name: gbuB [H]

Synonym: lwe0999

Alternate gene names: 116872417

Gene position: 1020110-1020958 (Clockwise)

Preceding gene: 116872416

Following gene: 116872418

Centisome position: 36.25

GC content: 39.69

Gene sequence:

>849_bases
ATGCCTAATATTCCAACGATTCCATTAGCTAGTTGGATTGATAAGTTAGTTGATGGCTTAACGCAATTTGAAGGATTTTT
TAATGTTATTACAAATATGATTGGTGGGGTTGTTGACGCATTTCAATGGGTCTTTGACTTAGTTCCACCATGGTTATTTA
TTATTTTACTTGTACTTGGAACATTTTGGGTTAATCGCAAAGGTAAAAAGTGGGGTTTAATCACTTTTGAAGTTGTCGGT
TTACTATTAATTTGGAACTTAGACTTTTGGCGTGATATGACACAGACCTTAACACTTGTACTTACAAGTAGTTTAATTGC
CCTTGTCATTGGTGTTCCACTTGGTATTTGGATGGCAAAAAGCTCTATTGTTGAAAGTATTTTTAAACCAGTGCTCGACT
TTATGCAAACAATGCCAGCCTTTGTATACTTAATTCCCGCGGTAGCATTTTTCGGTATTGGAATGGTTCCAGGGGTAGTT
GCTTCCGTTATTTTCGCAATGCCTCCAACTGTTCGTATGACTAACTTAGGTATTCGCCAAGTTTCTACAGAGCTTGTTGA
AGCGGCAGATTCTTTTGGTTCCACCCCTTGGCAAAAACTGTGGAAAGTCCAGCTTCCGATGGCTAAATCAACCATGATGG
CCGGAATTAACCAAAGTATTATGTTAGCACTTTCCATGGTTGTAATTGCTTCAATGATTGGTGCAATGGGGCTTGGGACA
CGTGTTTACTTTGCAGTAGGGCGTAACGACGCTGGTGGCGGATTTGTTGCCGGGATTGCGATTGTTATCGTAGCAATCAT
CCTGGACCGTTTGACACAAGCTTTCAATAAAAAAGCGAAATCAGAATAA

Upstream 100 bases:

>100_bases
ACGACAATTCCAATTGCTGTGACAGAAGATGGAAAACTAAAAGGAATTATTATTCGCGGATCCGTTCTGGCCGCACTTTC
TGGAAACGAGGTGAACGTTA

Downstream 100 bases:

>100_bases
GGAGGAGAGTAGATTGCTTAAAAAAATAATTAGCACTGCAGTTCTAGCTATGCTTATTTTCACGTTAGCAGCCTGCGGGA
CGACCCTTGCTCCTTATGAT

Product: glycine betaine/L-proline ABC transporter, permease protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MPNIPTIPLASWIDKLVDGLTQFEGFFNVITNMIGGVVDAFQWVFDLVPPWLFIILLVLGTFWVNRKGKKWGLITFEVVG
LLLIWNLDFWRDMTQTLTLVLTSSLIALVIGVPLGIWMAKSSIVESIFKPVLDFMQTMPAFVYLIPAVAFFGIGMVPGVV
ASVIFAMPPTVRMTNLGIRQVSTELVEAADSFGSTPWQKLWKVQLPMAKSTMMAGINQSIMLALSMVVIASMIGAMGLGT
RVYFAVGRNDAGGGFVAGIAIVIVAIILDRLTQAFNKKAKSE

Sequences:

>Translated_282_residues
MPNIPTIPLASWIDKLVDGLTQFEGFFNVITNMIGGVVDAFQWVFDLVPPWLFIILLVLGTFWVNRKGKKWGLITFEVVG
LLLIWNLDFWRDMTQTLTLVLTSSLIALVIGVPLGIWMAKSSIVESIFKPVLDFMQTMPAFVYLIPAVAFFGIGMVPGVV
ASVIFAMPPTVRMTNLGIRQVSTELVEAADSFGSTPWQKLWKVQLPMAKSTMMAGINQSIMLALSMVVIASMIGAMGLGT
RVYFAVGRNDAGGGFVAGIAIVIVAIILDRLTQAFNKKAKSE
>Mature_281_residues
PNIPTIPLASWIDKLVDGLTQFEGFFNVITNMIGGVVDAFQWVFDLVPPWLFIILLVLGTFWVNRKGKKWGLITFEVVGL
LLIWNLDFWRDMTQTLTLVLTSSLIALVIGVPLGIWMAKSSIVESIFKPVLDFMQTMPAFVYLIPAVAFFGIGMVPGVVA
SVIFAMPPTVRMTNLGIRQVSTELVEAADSFGSTPWQKLWKVQLPMAKSTMMAGINQSIMLALSMVVIASMIGAMGLGTR
VYFAVGRNDAGGGFVAGIAIVIVAIILDRLTQAFNKKAKSE

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=277, Percent_Identity=48.014440433213, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1788451, Length=201, Percent_Identity=30.8457711442786, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788449, Length=190, Percent_Identity=30.5263157894737, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786564, Length=155, Percent_Identity=28.3870967741935, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30850; Mature: 30719

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PNIPTIPLASWIDKLVDGLTQFEGFFNVITNMIGGVVDAFQWVFDLVPPWLFIILLVLG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFWVNRKGKKWGLITFEVVGLLLIWNLDFWRDMTQTLTLVLTSSLIALVIGVPLGIWMAK
HHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSIVESIFKPVLDFMQTMPAFVYLIPAVAFFGIGMVPGVVASVIFAMPPTVRMTNLGIRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VSTELVEAADSFGSTPWQKLWKVQLPMAKSTMMAGINQSIMLALSMVVIASMIGAMGLGT
HHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
RVYFAVGRNDAGGGFVAGIAIVIVAIILDRLTQAFNKKAKSE
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]