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The map label for this gene is ykwC [H]

Identifier: 116872407

GI number: 116872407

Start: 1011156

End: 1012016

Strand: Direct

Name: ykwC [H]

Synonym: lwe0989

Alternate gene names: 116872407

Gene position: 1011156-1012016 (Clockwise)

Preceding gene: 116872406

Following gene: 116872409

Centisome position: 35.93

GC content: 39.84

Gene sequence:

>861_bases
ATGAAAAAAATTGGGTTTGTTGGTACGGGAGTTATGGGTTCAAGTATGGCGAGTCATTTACTTGAAGCTGGTTATGACGT
ATTTATTTATACTCGTACTAAATCAAAGGCAGAAGAGCTACTAACAAAAGGTGCAAAATGGATAGATACTCCTCAAGCGC
TTGCGAAAAAGGTTGATGTACTAATTTCCATGGTTGGTTATCCAAAAGACGTGGAGGAACTTTACTTAGGTCAAAATGGC
TTTTTAGAGAATCTTTCTGCTGGTGCTGTGGCGATTGATATGACAACTTCTTCTCCTGTATTAGCTAAAAAAATTGCCGA
AGCTTTTCGTGAAAAGGGGATAGGTGCTTTAGATGCTCCCGTTTCAGGTGGCGATATTGGAGCGAAAAATGGGACGTTAG
CAATTATGGTTGGTGGAGCAGAAGACGTATTTTTGAAAGTGAAACCGATTTTTGAAATTCTTGGCAGTAGTGTCATTTTG
CAAGGCGATGCAGGTGCCGGACAACATACGAAAATGGTCAATCAAATTGCAATTGCATCTAATATGATTGGTGTAACAGA
AGCGATAATTTATACTGAGGCGGCTGGGCTTAATCCTGCGCGCGTATTAGAATCGATTTCAGGTGGAGCAGCTGGTAGTT
GGTCTCTCTCAAACTTGATTCCCCGTGTGCTTCAAGATGATTTTTCTCCTGGGTTCTTTATTAAACATTTTATTAAAGAT
ATGGGAATCGCTCTTTCGGAAGCGAAACAAATGGGGCTTGAACTTCCAGGACTATCCTTAGCAGAAAAAATGTATCAAAC
TTTAGCTGACCAAGGATTAAGTGAGGAAGGAACACAAGCTTTAATTAAATATTATCGCTAA

Upstream 100 bases:

>100_bases
ATGTACAAAAAAGCACATATGATTTTACTTGGAATACTTGTTTTATTCTTTGTATTTCTAGCGATTATGGGAGTTACATT
TAGTTAGGAGGTTTTTTAAA

Downstream 100 bases:

>100_bases
AAGTAAAACAACCACTACAAAAAATAGTGGTTGTTTGTTTATTTTGCTAAAAAAGTAGCCATACGTTCAAGTGCTTCCGC
AAGATTATTAATAGATGTCG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKKIGFVGTGVMGSSMASHLLEAGYDVFIYTRTKSKAEELLTKGAKWIDTPQALAKKVDVLISMVGYPKDVEELYLGQNG
FLENLSAGAVAIDMTTSSPVLAKKIAEAFREKGIGALDAPVSGGDIGAKNGTLAIMVGGAEDVFLKVKPIFEILGSSVIL
QGDAGAGQHTKMVNQIAIASNMIGVTEAIIYTEAAGLNPARVLESISGGAAGSWSLSNLIPRVLQDDFSPGFFIKHFIKD
MGIALSEAKQMGLELPGLSLAEKMYQTLADQGLSEEGTQALIKYYR

Sequences:

>Translated_286_residues
MKKIGFVGTGVMGSSMASHLLEAGYDVFIYTRTKSKAEELLTKGAKWIDTPQALAKKVDVLISMVGYPKDVEELYLGQNG
FLENLSAGAVAIDMTTSSPVLAKKIAEAFREKGIGALDAPVSGGDIGAKNGTLAIMVGGAEDVFLKVKPIFEILGSSVIL
QGDAGAGQHTKMVNQIAIASNMIGVTEAIIYTEAAGLNPARVLESISGGAAGSWSLSNLIPRVLQDDFSPGFFIKHFIKD
MGIALSEAKQMGLELPGLSLAEKMYQTLADQGLSEEGTQALIKYYR
>Mature_286_residues
MKKIGFVGTGVMGSSMASHLLEAGYDVFIYTRTKSKAEELLTKGAKWIDTPQALAKKVDVLISMVGYPKDVEELYLGQNG
FLENLSAGAVAIDMTTSSPVLAKKIAEAFREKGIGALDAPVSGGDIGAKNGTLAIMVGGAEDVFLKVKPIFEILGSSVIL
QGDAGAGQHTKMVNQIAIASNMIGVTEAIIYTEAAGLNPARVLESISGGAAGSWSLSNLIPRVLQDDFSPGFFIKHFIKD
MGIALSEAKQMGLELPGLSLAEKMYQTLADQGLSEEGTQALIKYYR

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=31.6151202749141, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI40556376, Length=284, Percent_Identity=26.056338028169, Blast_Score=125, Evalue=3e-29,
Organism=Escherichia coli, GI145693186, Length=284, Percent_Identity=38.7323943661972, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1786719, Length=281, Percent_Identity=39.8576512455516, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1790315, Length=288, Percent_Identity=31.9444444444444, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1789092, Length=286, Percent_Identity=28.3216783216783, Blast_Score=108, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17557316, Length=278, Percent_Identity=26.6187050359712, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24655230, Length=282, Percent_Identity=34.3971631205674, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI19922568, Length=282, Percent_Identity=34.3971631205674, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI28574115, Length=285, Percent_Identity=23.859649122807, Blast_Score=100, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30238; Mature: 30238

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIGFVGTGVMGSSMASHLLEAGYDVFIYTRTKSKAEELLTKGAKWIDTPQALAKKVDV
CCCCCCEECCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHH
LISMVGYPKDVEELYLGQNGFLENLSAGAVAIDMTTSSPVLAKKIAEAFREKGIGALDAP
HHHHHCCCCCHHHHHCCCCCCHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCC
VSGGDIGAKNGTLAIMVGGAEDVFLKVKPIFEILGSSVILQGDAGAGQHTKMVNQIAIAS
CCCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHH
NMIGVTEAIIYTEAAGLNPARVLESISGGAAGSWSLSNLIPRVLQDDFSPGFFIKHFIKD
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
MGIALSEAKQMGLELPGLSLAEKMYQTLADQGLSEEGTQALIKYYR
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKKIGFVGTGVMGSSMASHLLEAGYDVFIYTRTKSKAEELLTKGAKWIDTPQALAKKVDV
CCCCCCEECCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHH
LISMVGYPKDVEELYLGQNGFLENLSAGAVAIDMTTSSPVLAKKIAEAFREKGIGALDAP
HHHHHCCCCCHHHHHCCCCCCHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCC
VSGGDIGAKNGTLAIMVGGAEDVFLKVKPIFEILGSSVILQGDAGAGQHTKMVNQIAIAS
CCCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHH
NMIGVTEAIIYTEAAGLNPARVLESISGGAAGSWSLSNLIPRVLQDDFSPGFFIKHFIKD
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
MGIALSEAKQMGLELPGLSLAEKMYQTLADQGLSEEGTQALIKYYR
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]