Click here to switch to the map view.

The map label for this gene is ptsI [H]

Identifier: 116872405

GI number: 116872405

Start: 1008279

End: 1009997

Strand: Direct

Name: ptsI [H]

Synonym: lwe0987

Alternate gene names: 116872405

Gene position: 1008279-1009997 (Clockwise)

Preceding gene: 116872404

Following gene: 116872406

Centisome position: 35.83

GC content: 38.28

Gene sequence:

>1719_bases
ATGGCTAAAGAGTTGAAAGGTATCGCAGCATCTGATGGGATTGCCATTGCGAAAGCTTATCTGCTCGTTGAACCTGATCT
TTCCTATGAAAAAACAGAAGTTACGGATGTTGAAAGTGAAGTAAAACGTTTTGAAAGCGCGTTAGAAGTTTCTAGAACAG
AACTTTCAACGATTCGTGAAAAAGCAGCTAAAGACTTAGGCGAAGATAAGGCACAAATTTTTGACGCGCATCTTCTAGTT
TTAAATGATCCTGAATTAACTGGACCAATTGAAGAAAGCATCAAAAATGCTAAAACGAATGCAGAAACAGCTTTACAAGA
AACGACAGACATGTTTATTGGTATGTTTGAATCTATGGACAACGAATACATGCGTGAACGTGCAGCTGATATTAAGGATG
TACGCAAACGTGTTCTTTCTCACTTACTAGGTGTGACTATTCCAAATCCAGCTTTAATTGATGAGGAAGTAGTTGTTGTC
GCAGCTGATTTAACGCCTTCTGATACCGCACAACTTAACCGTAAATTTGTTAAAGGTTTTGTGACGGATATTGGTGGTCG
TACTTCGCATTCTGCTATTATGGCGCGTTCTCTTGAAATTCCAGCAGTAGTTGGAACTAAAGAAGTAACTGCTAGTGTAG
CTAAGAACGATATGGTTATTATTGATGGTTTGGAAGGTAATGTTATCATCCATCCAACAGAAGAACAAATCGCTCACTAT
GAAAAAATTAAATCTGATTTTGCTTTACAACAAGCAGAATGGGAAAAACTTAAAAATGAAAAAACGGTTTCTAAAGATGG
TGTTCACGTGGAGCTTGCAGCAAACATCGGAACTCCAAATGATTTAGAAGGTGTTATTTCTAATGGTGGGGAAGCAGTTG
GACTTTATCGTACAGAATTCTTGTACATGGGTCGTGACAATTTCCCAACAGAAGAAGAGCAATTCGAAGCGTATAAAGCA
GTAGTTTCCGGAATGGACGGAAAATCTGTGGTCGTTCGTACTTTAGATATCGGTGGCGATAAAACGTTACCATACCTTGA
ACTTCCTGAAGAAATGAACCCATTCTTAGGTTTCCGTGCAATTCGTCTTTGTTTTGCAAATGAAGAATTATTCCGTACAC
AACTTCGCGCATTACTTCGCGCAAGTGTTTATGGTAACTTAAAAATTATGTTCCCGATGATTGCAACAGTCAATGAATTC
CGCCAAGCGCGCGATATTTTACTTGATGAAAAAGCAAAACTAAAAGCCGCTGGGACAGAAGTTTCTGATTCCATCGAACT
TGGAATTATGATTGAGATTCCTGCTGCTGCAGTTCTTGCTGATCAATTTGCTAAAGAAGTTGATTTCTTCTCTATCGGAA
CTAATGATTTAATTCAGTATACAATGGCTGCTGACCGTATGAACGAACGTGTTTCTTATCTTTACCAACCATACAATCCA
TCCATTCTACGTTTAGTAAAAATGGTGATTGATGCTTCTCATAAAGAAGGTAAATGGACTGGTATGTGCGGAGAAATGGC
TGGAGATCAAACGGCTGTACCACTTCTTTTAGGCCTAGGCTTAGATGAATTTTCAATGAGTGCTTCAAGTATTCTTAAGT
CTCGTTCGTTAATCAAACGTTTGGATCAATCTGAAATGGTGAAATTAGCGGAAGAAGCTTTAAATAAAGCTACAGCTGAA
GAAGTTGTAGAATTAGTTGAAAAATATACTGCAGAGTAA

Upstream 100 bases:

>100_bases
GTAAAGGCGCTGACATCACTATTTATACTGAAGGTAGCGACGAAAAAGAAGCAATTGAAGGACTAACTGAAGTTCTTAAG
AAAGAAGGATTGGCTGAATA

Downstream 100 bases:

>100_bases
TTTCTGAGAGTGACCTAGACGAATTTATTCGTCTAGGTCTTTTTGTATTGTGTAAATGAGGTAGCGCAAGTATAATAAAA
AGACAGTTAGCTTTAAAGGG

Product: phosphotransferase system enzyme I

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MAKELKGIAASDGIAIAKAYLLVEPDLSYEKTEVTDVESEVKRFESALEVSRTELSTIREKAAKDLGEDKAQIFDAHLLV
LNDPELTGPIEESIKNAKTNAETALQETTDMFIGMFESMDNEYMRERAADIKDVRKRVLSHLLGVTIPNPALIDEEVVVV
AADLTPSDTAQLNRKFVKGFVTDIGGRTSHSAIMARSLEIPAVVGTKEVTASVAKNDMVIIDGLEGNVIIHPTEEQIAHY
EKIKSDFALQQAEWEKLKNEKTVSKDGVHVELAANIGTPNDLEGVISNGGEAVGLYRTEFLYMGRDNFPTEEEQFEAYKA
VVSGMDGKSVVVRTLDIGGDKTLPYLELPEEMNPFLGFRAIRLCFANEELFRTQLRALLRASVYGNLKIMFPMIATVNEF
RQARDILLDEKAKLKAAGTEVSDSIELGIMIEIPAAAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNP
SILRLVKMVIDASHKEGKWTGMCGEMAGDQTAVPLLLGLGLDEFSMSASSILKSRSLIKRLDQSEMVKLAEEALNKATAE
EVVELVEKYTAE

Sequences:

>Translated_572_residues
MAKELKGIAASDGIAIAKAYLLVEPDLSYEKTEVTDVESEVKRFESALEVSRTELSTIREKAAKDLGEDKAQIFDAHLLV
LNDPELTGPIEESIKNAKTNAETALQETTDMFIGMFESMDNEYMRERAADIKDVRKRVLSHLLGVTIPNPALIDEEVVVV
AADLTPSDTAQLNRKFVKGFVTDIGGRTSHSAIMARSLEIPAVVGTKEVTASVAKNDMVIIDGLEGNVIIHPTEEQIAHY
EKIKSDFALQQAEWEKLKNEKTVSKDGVHVELAANIGTPNDLEGVISNGGEAVGLYRTEFLYMGRDNFPTEEEQFEAYKA
VVSGMDGKSVVVRTLDIGGDKTLPYLELPEEMNPFLGFRAIRLCFANEELFRTQLRALLRASVYGNLKIMFPMIATVNEF
RQARDILLDEKAKLKAAGTEVSDSIELGIMIEIPAAAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNP
SILRLVKMVIDASHKEGKWTGMCGEMAGDQTAVPLLLGLGLDEFSMSASSILKSRSLIKRLDQSEMVKLAEEALNKATAE
EVVELVEKYTAE
>Mature_571_residues
AKELKGIAASDGIAIAKAYLLVEPDLSYEKTEVTDVESEVKRFESALEVSRTELSTIREKAAKDLGEDKAQIFDAHLLVL
NDPELTGPIEESIKNAKTNAETALQETTDMFIGMFESMDNEYMRERAADIKDVRKRVLSHLLGVTIPNPALIDEEVVVVA
ADLTPSDTAQLNRKFVKGFVTDIGGRTSHSAIMARSLEIPAVVGTKEVTASVAKNDMVIIDGLEGNVIIHPTEEQIAHYE
KIKSDFALQQAEWEKLKNEKTVSKDGVHVELAANIGTPNDLEGVISNGGEAVGLYRTEFLYMGRDNFPTEEEQFEAYKAV
VSGMDGKSVVVRTLDIGGDKTLPYLELPEEMNPFLGFRAIRLCFANEELFRTQLRALLRASVYGNLKIMFPMIATVNEFR
QARDILLDEKAKLKAAGTEVSDSIELGIMIEIPAAAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPS
ILRLVKMVIDASHKEGKWTGMCGEMAGDQTAVPLLLGLGLDEFSMSASSILKSRSLIKRLDQSEMVKLAEEALNKATAEE
VVELVEKYTAE

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=570, Percent_Identity=50.7017543859649, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI1788726, Length=538, Percent_Identity=37.360594795539, Blast_Score=355, Evalue=4e-99,
Organism=Escherichia coli, GI48994992, Length=501, Percent_Identity=36.9261477045908, Blast_Score=345, Evalue=5e-96,
Organism=Escherichia coli, GI1789193, Length=579, Percent_Identity=31.9516407599309, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1787994, Length=423, Percent_Identity=27.1867612293144, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI226510935, Length=186, Percent_Identity=29.0322580645161, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63197; Mature: 63066

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKELKGIAASDGIAIAKAYLLVEPDLSYEKTEVTDVESEVKRFESALEVSRTELSTIRE
CCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAAKDLGEDKAQIFDAHLLVLNDPELTGPIEESIKNAKTNAETALQETTDMFIGMFESMD
HHHHHCCCHHHHHEEEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
NEYMRERAADIKDVRKRVLSHLLGVTIPNPALIDEEVVVVAADLTPSDTAQLNRKFVKGF
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
VTDIGGRTSHSAIMARSLEIPAVVGTKEVTASVAKNDMVIIDGLEGNVIIHPTEEQIAHY
HHHCCCCCCHHHHHHHCCCCCEEECCHHHHHHHHCCCEEEEECCCCCEEEECCHHHHHHH
EKIKSDFALQQAEWEKLKNEKTVSKDGVHVELAANIGTPNDLEGVISNGGEAVGLYRTEF
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCEEEEEEEEE
LYMGRDNFPTEEEQFEAYKAVVSGMDGKSVVVRTLDIGGDKTLPYLELPEEMNPFLGFRA
EEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEECCHHCCHHHHHHH
IRLCFANEELFRTQLRALLRASVYGNLKIMFPMIATVNEFRQARDILLDEKAKLKAAGTE
HHHHHCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
VSDSIELGIMIEIPAAAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNP
CCCCEEEEEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCH
SILRLVKMVIDASHKEGKWTGMCGEMAGDQTAVPLLLGLGLDEFSMSASSILKSRSLIKR
HHHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LDQSEMVKLAEEALNKATAEEVVELVEKYTAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKELKGIAASDGIAIAKAYLLVEPDLSYEKTEVTDVESEVKRFESALEVSRTELSTIRE
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAAKDLGEDKAQIFDAHLLVLNDPELTGPIEESIKNAKTNAETALQETTDMFIGMFESMD
HHHHHCCCHHHHHEEEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
NEYMRERAADIKDVRKRVLSHLLGVTIPNPALIDEEVVVVAADLTPSDTAQLNRKFVKGF
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
VTDIGGRTSHSAIMARSLEIPAVVGTKEVTASVAKNDMVIIDGLEGNVIIHPTEEQIAHY
HHHCCCCCCHHHHHHHCCCCCEEECCHHHHHHHHCCCEEEEECCCCCEEEECCHHHHHHH
EKIKSDFALQQAEWEKLKNEKTVSKDGVHVELAANIGTPNDLEGVISNGGEAVGLYRTEF
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCEEEEEEEEE
LYMGRDNFPTEEEQFEAYKAVVSGMDGKSVVVRTLDIGGDKTLPYLELPEEMNPFLGFRA
EEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEECCHHCCHHHHHHH
IRLCFANEELFRTQLRALLRASVYGNLKIMFPMIATVNEFRQARDILLDEKAKLKAAGTE
HHHHHCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
VSDSIELGIMIEIPAAAVLADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNP
CCCCEEEEEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCH
SILRLVKMVIDASHKEGKWTGMCGEMAGDQTAVPLLLGLGLDEFSMSASSILKSRSLIKR
HHHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LDQSEMVKLAEEALNKATAEEVVELVEKYTAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]