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The map label for this gene is prfC [H]

Identifier: 116872389

GI number: 116872389

Start: 992959

End: 994527

Strand: Direct

Name: prfC [H]

Synonym: lwe0971

Alternate gene names: 116872389

Gene position: 992959-994527 (Clockwise)

Preceding gene: 116872388

Following gene: 116872390

Centisome position: 35.28

GC content: 38.43

Gene sequence:

>1569_bases
ATGAGTCAAGATTTGCAAAAAGAAGTTGCTTCACGCAAAACGTTTGCGATTATTTCTCACCCGGATGCTGGGAAAACGAC
GATTACAGAACAATTATTATTATTCGGTGGCGTTATCCGTTCAGCTGGGACTGTAAAGGGAAAGAAATCTGGAAAGTTTG
CGACAAGTGACTGGATGGAAATTGAAAAACAACGTGGAATCTCGGTAACGAGTTCTGTTATGCAGTTTGATTATAATGGA
TCAAGAATTAATATTTTGGATACACCAGGGCACTCTGATTTCAGTGAAGATACGTATCGAACATTAATGGCAGTGGATAG
TGCTGTCATGGTTATTGATGCAGCCAAAGGTATTGAAGCACAAACCCTCAAATTATTCAAAGTTTGTCGGATGCGCGGAA
TTCCTATTTTTACGTTTATTAACAAAATGGACCGCCAAGGGAAAATGCCACTTGAACTCCTTGCTGAATTAGAAGAAGTT
TTAGAAATTGAATCGTATCCAATGAACTGGCCAATTGGTATGGGAAAAGAGCTTGCTGGGCTTTATGATCGTTATCATCG
TGTAATTGAACAATATCGCTCGGAAGAAGATGAGCGCTTCTTACCACTTGGTGAAGATGGTGATTTGAAGGAAGCGCACG
AAATTCAAAAATCACTCTATTATGATCAAGCATTGGAAGAAATAATGTTACTTGATGAAGCAGGTAATGATTTCAGCCGC
GAACGTATTCTTGCGGGAGAACAAACTCCAGTATTTTTTGGGAGTGCATTAACAAATTTTGGTGTAGAAACTTTCTTACG
TACCTTTGTTGATTTTGCGCCAGCCCCTTCAAGCCACGAATCTAATGAAGGCATTATTGAAGCAGACAATCCGAAATTCT
CTGGATTTATCTTCAAAATCCAAGCAAACATGAATCCTGCTCACCGAGATCGTATTGCTTTTATTCGAATTTGTTCAGGC
GAATTTGAGCGTGGAATGAATGTGACGTTAACCCGAACAGGAAAAAGTATCAAATTAGCTAATTCCACTCAGTTTATGGC
AGATGACAGGGAAACCGTTAATCGTGCTGTAGCCGGAGATATTATCGGTTTATACGACACTGGTAACTACCAAATTGGTG
ATACGATAACAAATGGAAGTAAAAAACTCGAATTTGAAAAACTTCCTCAGTTTACACCGGAATTATTTATGCGTGTTTAT
GCGAAAAATGTCATGAAACAAAAGCATTTCCATAAAGGTGTGGAGCAGCTCGTTCAAGAAGGTGCGATTCAATTATTTAA
AACATGGCGCACAGAGGAATACATTATTGGAGCTGTCGGTCAGTTGCAATTTGAAGTATTTGAACACAGAATGCGCGGCG
AATATAATTCTGAAATCCGGATGGAACCAATTGGTAAAAAAATCGCTCGTTGGGTAAAAGAAGAAGATGCGGATGAAAAA
CTATCCACAGCTAGAAGCATGCTAGTAAAAGATCGTTTCGACCAACCGTTATTTCTATTCGAAAATGAATTCGCGATTAA
TTGGTTTAATGATAAAAATCCAGATATCGAATTAACTTCATTATTATAA

Upstream 100 bases:

>100_bases
GAAACGACAAACTAAATAATTTTTGAATAAAAAAACAGCCGAAGTTCTCCTAATTATGCTATACTAATGGAATGTGAATG
AATCTAGGAGGAAAATAACA

Downstream 100 bases:

>100_bases
AATACGCAACCTATTTGTACTTTCGCAAATAGGTTGTTTTTTTCTTGACAATTTTTTAAAACGCGCTATAATTAGTTAGA
ATTCTAATTAATAAGGAGTT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MSQDLQKEVASRKTFAIISHPDAGKTTITEQLLLFGGVIRSAGTVKGKKSGKFATSDWMEIEKQRGISVTSSVMQFDYNG
SRINILDTPGHSDFSEDTYRTLMAVDSAVMVIDAAKGIEAQTLKLFKVCRMRGIPIFTFINKMDRQGKMPLELLAELEEV
LEIESYPMNWPIGMGKELAGLYDRYHRVIEQYRSEEDERFLPLGEDGDLKEAHEIQKSLYYDQALEEIMLLDEAGNDFSR
ERILAGEQTPVFFGSALTNFGVETFLRTFVDFAPAPSSHESNEGIIEADNPKFSGFIFKIQANMNPAHRDRIAFIRICSG
EFERGMNVTLTRTGKSIKLANSTQFMADDRETVNRAVAGDIIGLYDTGNYQIGDTITNGSKKLEFEKLPQFTPELFMRVY
AKNVMKQKHFHKGVEQLVQEGAIQLFKTWRTEEYIIGAVGQLQFEVFEHRMRGEYNSEIRMEPIGKKIARWVKEEDADEK
LSTARSMLVKDRFDQPLFLFENEFAINWFNDKNPDIELTSLL

Sequences:

>Translated_522_residues
MSQDLQKEVASRKTFAIISHPDAGKTTITEQLLLFGGVIRSAGTVKGKKSGKFATSDWMEIEKQRGISVTSSVMQFDYNG
SRINILDTPGHSDFSEDTYRTLMAVDSAVMVIDAAKGIEAQTLKLFKVCRMRGIPIFTFINKMDRQGKMPLELLAELEEV
LEIESYPMNWPIGMGKELAGLYDRYHRVIEQYRSEEDERFLPLGEDGDLKEAHEIQKSLYYDQALEEIMLLDEAGNDFSR
ERILAGEQTPVFFGSALTNFGVETFLRTFVDFAPAPSSHESNEGIIEADNPKFSGFIFKIQANMNPAHRDRIAFIRICSG
EFERGMNVTLTRTGKSIKLANSTQFMADDRETVNRAVAGDIIGLYDTGNYQIGDTITNGSKKLEFEKLPQFTPELFMRVY
AKNVMKQKHFHKGVEQLVQEGAIQLFKTWRTEEYIIGAVGQLQFEVFEHRMRGEYNSEIRMEPIGKKIARWVKEEDADEK
LSTARSMLVKDRFDQPLFLFENEFAINWFNDKNPDIELTSLL
>Mature_521_residues
SQDLQKEVASRKTFAIISHPDAGKTTITEQLLLFGGVIRSAGTVKGKKSGKFATSDWMEIEKQRGISVTSSVMQFDYNGS
RINILDTPGHSDFSEDTYRTLMAVDSAVMVIDAAKGIEAQTLKLFKVCRMRGIPIFTFINKMDRQGKMPLELLAELEEVL
EIESYPMNWPIGMGKELAGLYDRYHRVIEQYRSEEDERFLPLGEDGDLKEAHEIQKSLYYDQALEEIMLLDEAGNDFSRE
RILAGEQTPVFFGSALTNFGVETFLRTFVDFAPAPSSHESNEGIIEADNPKFSGFIFKIQANMNPAHRDRIAFIRICSGE
FERGMNVTLTRTGKSIKLANSTQFMADDRETVNRAVAGDIIGLYDTGNYQIGDTITNGSKKLEFEKLPQFTPELFMRVYA
KNVMKQKHFHKGVEQLVQEGAIQLFKTWRTEEYIIGAVGQLQFEVFEHRMRGEYNSEIRMEPIGKKIARWVKEEDADEKL
STARSMLVKDRFDQPLFLFENEFAINWFNDKNPDIELTSLL

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=480, Percent_Identity=28.75, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI19923640, Length=510, Percent_Identity=26.078431372549, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI25306283, Length=398, Percent_Identity=29.6482412060301, Blast_Score=149, Evalue=9e-36,
Organism=Homo sapiens, GI25306287, Length=294, Percent_Identity=32.312925170068, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI157426893, Length=161, Percent_Identity=32.2981366459627, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI94966754, Length=145, Percent_Identity=32.4137931034483, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI310132016, Length=121, Percent_Identity=33.8842975206612, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI310110807, Length=121, Percent_Identity=33.8842975206612, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI310123363, Length=121, Percent_Identity=33.8842975206612, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI4503483, Length=152, Percent_Identity=28.9473684210526, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1790835, Length=520, Percent_Identity=47.6923076923077, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1789738, Length=483, Percent_Identity=28.3643892339545, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI48994988, Length=458, Percent_Identity=23.7991266375546, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1788922, Length=159, Percent_Identity=30.188679245283, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1790412, Length=150, Percent_Identity=28, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1789737, Length=150, Percent_Identity=28, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17533571, Length=484, Percent_Identity=28.9256198347107, Blast_Score=198, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17556745, Length=134, Percent_Identity=41.7910447761194, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=31.8518518518519, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=172, Percent_Identity=29.6511627906977, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=33.0827067669173, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=33.0827067669173, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=473, Percent_Identity=28.3298097251586, Blast_Score=187, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6322359, Length=508, Percent_Identity=26.5748031496063, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6323320, Length=154, Percent_Identity=29.2207792207792, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=159, Percent_Identity=28.3018867924528, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=159, Percent_Identity=28.3018867924528, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=181, Percent_Identity=26.5193370165746, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24582462, Length=487, Percent_Identity=28.3367556468172, Blast_Score=194, Evalue=2e-49,
Organism=Drosophila melanogaster, GI221458488, Length=501, Percent_Identity=24.9500998003992, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI78706572, Length=157, Percent_Identity=31.2101910828025, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585711, Length=170, Percent_Identity=28.2352941176471, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24585713, Length=170, Percent_Identity=28.2352941176471, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24585709, Length=170, Percent_Identity=28.2352941176471, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59545; Mature: 59413

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDLQKEVASRKTFAIISHPDAGKTTITEQLLLFGGVIRSAGTVKGKKSGKFATSDWME
CCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
IEKQRGISVTSSVMQFDYNGSRINILDTPGHSDFSEDTYRTLMAVDSAVMVIDAAKGIEA
HHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCH
QTLKLFKVCRMRGIPIFTFINKMDRQGKMPLELLAELEEVLEIESYPMNWPIGMGKELAG
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
LYDRYHRVIEQYRSEEDERFLPLGEDGDLKEAHEIQKSLYYDQALEEIMLLDEAGNDFSR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ERILAGEQTPVFFGSALTNFGVETFLRTFVDFAPAPSSHESNEGIIEADNPKFSGFIFKI
HHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEEEE
QANMNPAHRDRIAFIRICSGEFERGMNVTLTRTGKSIKLANSTQFMADDRETVNRAVAGD
EECCCCCHHCCEEEEEEECCHHHCCCEEEEEECCCEEEEECCCCHHCCCHHHHHHHHHCC
IIGLYDTGNYQIGDTITNGSKKLEFEKLPQFTPELFMRVYAKNVMKQKHFHKGVEQLVQE
EEEEEECCCEEECCEECCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAIQLFKTWRTEEYIIGAVGQLQFEVFEHRMRGEYNSEIRMEPIGKKIARWVKEEDADEK
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHCCHHHH
LSTARSMLVKDRFDQPLFLFENEFAINWFNDKNPDIELTSLL
HHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCEEEEECC
>Mature Secondary Structure 
SQDLQKEVASRKTFAIISHPDAGKTTITEQLLLFGGVIRSAGTVKGKKSGKFATSDWME
CHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
IEKQRGISVTSSVMQFDYNGSRINILDTPGHSDFSEDTYRTLMAVDSAVMVIDAAKGIEA
HHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCH
QTLKLFKVCRMRGIPIFTFINKMDRQGKMPLELLAELEEVLEIESYPMNWPIGMGKELAG
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
LYDRYHRVIEQYRSEEDERFLPLGEDGDLKEAHEIQKSLYYDQALEEIMLLDEAGNDFSR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ERILAGEQTPVFFGSALTNFGVETFLRTFVDFAPAPSSHESNEGIIEADNPKFSGFIFKI
HHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEEEE
QANMNPAHRDRIAFIRICSGEFERGMNVTLTRTGKSIKLANSTQFMADDRETVNRAVAGD
EECCCCCHHCCEEEEEEECCHHHCCCEEEEEECCCEEEEECCCCHHCCCHHHHHHHHHCC
IIGLYDTGNYQIGDTITNGSKKLEFEKLPQFTPELFMRVYAKNVMKQKHFHKGVEQLVQE
EEEEEECCCEEECCEECCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAIQLFKTWRTEEYIIGAVGQLQFEVFEHRMRGEYNSEIRMEPIGKKIARWVKEEDADEK
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHCCHHHH
LSTARSMLVKDRFDQPLFLFENEFAINWFNDKNPDIELTSLL
HHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]