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The map label for this gene is ppnK [H]

Identifier: 116872368

GI number: 116872368

Start: 974800

End: 975594

Strand: Direct

Name: ppnK [H]

Synonym: lwe0950

Alternate gene names: 116872368

Gene position: 974800-975594 (Clockwise)

Preceding gene: 116872367

Following gene: 116872369

Centisome position: 34.64

GC content: 37.36

Gene sequence:

>795_bases
ATGAAATATATGATTACTTCAAAAGGGGACGAAAAGTCTGATTTACTGCGATTAAACATGATTGCAGGTTTTGGGGAATA
TGATATGGAATATGATGATATAGAACCTGAAATCGTTATTTCTATTGGCGGGGATGGAACTTTTCTGGCTGCTTTCCATC
AATATGAAGAACGGTTAGATGAGATAGCATTCATCGGTATCCATACGGGACATCTTGGTTTTTACGCGGATTGGAGACCA
GCTGAGGCGAATAAATTAGTGAAGCTTTTGGCAAAAGGTGAATACCAAAAAGTATCTTATCCACTTCTTAAAACTACAGT
AAAATACGGTGTTGGTAAAAAAGAAGCAGAATATTTAGCGCTAAATGAGTCAACTGTAAAAAGTTCAGGTGGACCTTTTG
TTGTTGATGTTGTAATTAATGACCTTCATTTTGAACGATTCCGTGGTGATGGGCTTTGTATGTCGACACCAAGCGGAACG
ACCGCCTATAATAAATCGCTTGGAGGAGCGTTAATGCATCCTTCTATTGAAGCAATGCAATTAACGGAAATGGCATCTAT
TAATAACCGTGTGTATCGTACAATTGGTAGCCCGTTAGTTTTTCCGAAGCACCATATTGTAAGTTTGCAACCGGTAAACG
ATAAAGATTTTCAAATTTCAGTGGACCATTTAAGTATTTTGCATCGTGATGTTCAAGAAATTCGCTATGAAGTATCTCCA
AAGAAAATTCAATTTGCGAGATTTAGATCATTTCCGTTTTGGCGTCGAGTGCACGATTCATTTATTGAAGATTAA

Upstream 100 bases:

>100_bases
TCTCAAAATAAAGATGTATCAAAGGATAAAAAAGCGGTACATCCAGTAATGCCAAAGAAAAATAAGTAATAAGACGTAGG
AATAGGGGCGAATAGAAATA

Downstream 100 bases:

>100_bases
GCTAGCAGGAGGAAGCATGTGTTTTTAGAATGGCAAGTAGAAAATGAAGAAGATGGATTATTGCTTAGAACTTTTCTTAG
AAGTAAACATATTTCCAAGC

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 1 [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKYMITSKGDEKSDLLRLNMIAGFGEYDMEYDDIEPEIVISIGGDGTFLAAFHQYEERLDEIAFIGIHTGHLGFYADWRP
AEANKLVKLLAKGEYQKVSYPLLKTTVKYGVGKKEAEYLALNESTVKSSGGPFVVDVVINDLHFERFRGDGLCMSTPSGT
TAYNKSLGGALMHPSIEAMQLTEMASINNRVYRTIGSPLVFPKHHIVSLQPVNDKDFQISVDHLSILHRDVQEIRYEVSP
KKIQFARFRSFPFWRRVHDSFIED

Sequences:

>Translated_264_residues
MKYMITSKGDEKSDLLRLNMIAGFGEYDMEYDDIEPEIVISIGGDGTFLAAFHQYEERLDEIAFIGIHTGHLGFYADWRP
AEANKLVKLLAKGEYQKVSYPLLKTTVKYGVGKKEAEYLALNESTVKSSGGPFVVDVVINDLHFERFRGDGLCMSTPSGT
TAYNKSLGGALMHPSIEAMQLTEMASINNRVYRTIGSPLVFPKHHIVSLQPVNDKDFQISVDHLSILHRDVQEIRYEVSP
KKIQFARFRSFPFWRRVHDSFIED
>Mature_264_residues
MKYMITSKGDEKSDLLRLNMIAGFGEYDMEYDDIEPEIVISIGGDGTFLAAFHQYEERLDEIAFIGIHTGHLGFYADWRP
AEANKLVKLLAKGEYQKVSYPLLKTTVKYGVGKKEAEYLALNESTVKSSGGPFVVDVVINDLHFERFRGDGLCMSTPSGT
TAYNKSLGGALMHPSIEAMQLTEMASINNRVYRTIGSPLVFPKHHIVSLQPVNDKDFQISVDHLSILHRDVQEIRYEVSP
KKIQFARFRSFPFWRRVHDSFIED

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=189, Percent_Identity=29.1005291005291, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320794, Length=165, Percent_Identity=27.8787878787879, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 29976; Mature: 29976

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYMITSKGDEKSDLLRLNMIAGFGEYDMEYDDIEPEIVISIGGDGTFLAAFHQYEERLD
CCEEEECCCCCHHHEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHH
EIAFIGIHTGHLGFYADWRPAEANKLVKLLAKGEYQKVSYPLLKTTVKYGVGKKEAEYLA
HEEEEEEEECCCCEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCEEEE
LNESTVKSSGGPFVVDVVINDLHFERFRGDGLCMSTPSGTTAYNKSLGGALMHPSIEAMQ
ECCCHHHCCCCCEEEEEEECHHHHHHHCCCCEEEECCCCCCHHHHCCCCEEECCCHHHHH
LTEMASINNRVYRTIGSPLVFPKHHIVSLQPVNDKDFQISVDHLSILHRDVQEIRYEVSP
HHHHHHHCCHHHHHCCCCEECCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCC
KKIQFARFRSFPFWRRVHDSFIED
CEEEHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKYMITSKGDEKSDLLRLNMIAGFGEYDMEYDDIEPEIVISIGGDGTFLAAFHQYEERLD
CCEEEECCCCCHHHEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHH
EIAFIGIHTGHLGFYADWRPAEANKLVKLLAKGEYQKVSYPLLKTTVKYGVGKKEAEYLA
HEEEEEEEECCCCEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCEEEE
LNESTVKSSGGPFVVDVVINDLHFERFRGDGLCMSTPSGTTAYNKSLGGALMHPSIEAMQ
ECCCHHHCCCCCEEEEEEECHHHHHHHCCCCEEEECCCCCCHHHHCCCCEEECCCHHHHH
LTEMASINNRVYRTIGSPLVFPKHHIVSLQPVNDKDFQISVDHLSILHRDVQEIRYEVSP
HHHHHHHCCHHHHHCCCCEECCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCC
KKIQFARFRSFPFWRRVHDSFIED
CEEEHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]