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The map label for this gene is lplA [H]

Identifier: 116872329

GI number: 116872329

Start: 941498

End: 942493

Strand: Direct

Name: lplA [H]

Synonym: lwe0911

Alternate gene names: 116872329

Gene position: 941498-942493 (Clockwise)

Preceding gene: 116872328

Following gene: 116872331

Centisome position: 33.46

GC content: 34.14

Gene sequence:

>996_bases
ATGTATTTTATAGATAATAATAATGAAAAAGATCCACGGATTAATTTGGCGGTAGAGGAATTTATTTTGACAGAGCTAGA
GTTAGATGAGCCTGTGCTGCTTTTCTACATCAATAAGCCATCGATTATTATTGGCCGGAATCAAAATACAGTAGAAGAAA
TTGATACAGAATATGTCGAAAAAAATGATGTAATCGTTGTGCGGAGACTTTCCGGCGGTGGCGCGGTTTATCATGATGAA
GGTAATTTGAATTTTAGTTTTATCACGGAGGACGACGGTGAATCTTTCCATAATTTCGCTAAATTTACACAGCCAATTGT
AGAAGCGCTGAAACGTTTAGGAGTAAATGCGGAACTAAAAGGGCGAAATGATTTATTAATTGATGGTTTTAAAGTCTCTG
GAAATGCCCAATTTGCAACAAAAGGAAAAATGTTTTCACATGGCACATTAATGTATGATTTGAACTTGGATAATGTCGCT
GCATCGCTAAAACCACGTAAAGATAAAATTGAATCAAAAGGAATTAAGTCTGTTCGTAGTCGTGTAGCAAATATTTCTGA
TTTTATGGATCAAGAAATGACAACAGAGGAATTCCGTGATTTGTTATTACTATATATTTTTGATGTAGATAAAGTAGAAG
ATGTAAAAGAATATAAGTTAACTGACGCAGATTGGGAAAAAATCCATGAAATTTCTGCTAAGCGTTATGGGAACTGGGAC
TGGAATTACGGTAAATCGCCAAAATTCGATTTAACACGGACTAAACGTTTTCCAGTCGGAGCTGTCGATGTTCGCTTAAA
TGTTCAAAAAGGAATTATTACAGAGATTAAGATTTTCGGTGATTTCTTTGGAGTGAAAAATGTGGCAGATATTGAAGCGA
AGTTAGTAAATACTACTTACAAACGTGAAGCTTTGGCGGAAGCTTTGAGTGATATTGAAGTAAAAGAATACTTTGGTAAT
ATTACAAAAGACGAATTTTTAGATTTACTTTATTAA

Upstream 100 bases:

>100_bases
TGTTGAAGCAAGAAGCAGCAGCTATTTATACTGGAGAAATTGCGCTTGCATCAAAAGGATTTACCAAAACATTTTAATAT
AGAAGAAAAGGGGTAGGAGT

Downstream 100 bases:

>100_bases
AAAAACGGAAAGCACGACTTACTTGTGCTTTCCGTTTTTATTTGAGATTTTTTCCTGACATCCAATGTTCTAGCCGTTTT
CCGACAAACCATAAAATAAC

Product: lipoyltransferase and lipoate-protein ligase family protein

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MYFIDNNNEKDPRINLAVEEFILTELELDEPVLLFYINKPSIIIGRNQNTVEEIDTEYVEKNDVIVVRRLSGGGAVYHDE
GNLNFSFITEDDGESFHNFAKFTQPIVEALKRLGVNAELKGRNDLLIDGFKVSGNAQFATKGKMFSHGTLMYDLNLDNVA
ASLKPRKDKIESKGIKSVRSRVANISDFMDQEMTTEEFRDLLLLYIFDVDKVEDVKEYKLTDADWEKIHEISAKRYGNWD
WNYGKSPKFDLTRTKRFPVGAVDVRLNVQKGIITEIKIFGDFFGVKNVADIEAKLVNTTYKREALAEALSDIEVKEYFGN
ITKDEFLDLLY

Sequences:

>Translated_331_residues
MYFIDNNNEKDPRINLAVEEFILTELELDEPVLLFYINKPSIIIGRNQNTVEEIDTEYVEKNDVIVVRRLSGGGAVYHDE
GNLNFSFITEDDGESFHNFAKFTQPIVEALKRLGVNAELKGRNDLLIDGFKVSGNAQFATKGKMFSHGTLMYDLNLDNVA
ASLKPRKDKIESKGIKSVRSRVANISDFMDQEMTTEEFRDLLLLYIFDVDKVEDVKEYKLTDADWEKIHEISAKRYGNWD
WNYGKSPKFDLTRTKRFPVGAVDVRLNVQKGIITEIKIFGDFFGVKNVADIEAKLVNTTYKREALAEALSDIEVKEYFGN
ITKDEFLDLLY
>Mature_331_residues
MYFIDNNNEKDPRINLAVEEFILTELELDEPVLLFYINKPSIIIGRNQNTVEEIDTEYVEKNDVIVVRRLSGGGAVYHDE
GNLNFSFITEDDGESFHNFAKFTQPIVEALKRLGVNAELKGRNDLLIDGFKVSGNAQFATKGKMFSHGTLMYDLNLDNVA
ASLKPRKDKIESKGIKSVRSRVANISDFMDQEMTTEEFRDLLLLYIFDVDKVEDVKEYKLTDADWEKIHEISAKRYGNWD
WNYGKSPKFDLTRTKRFPVGAVDVRLNVQKGIITEIKIFGDFFGVKNVADIEAKLVNTTYKREALAEALSDIEVKEYFGN
ITKDEFLDLLY

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=241, Percent_Identity=31.1203319502075, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI21729884, Length=241, Percent_Identity=31.1203319502075, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI21729882, Length=241, Percent_Identity=31.1203319502075, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI21729880, Length=241, Percent_Identity=31.1203319502075, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI21729878, Length=241, Percent_Identity=31.1203319502075, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI1790846, Length=304, Percent_Identity=34.5394736842105, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI71981909, Length=255, Percent_Identity=35.2941176470588, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322415, Length=193, Percent_Identity=33.160621761658, Blast_Score=105, Evalue=7e-24,
Organism=Drosophila melanogaster, GI28573464, Length=283, Percent_Identity=26.5017667844523, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38005; Mature: 38005

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFIDNNNEKDPRINLAVEEFILTELELDEPVLLFYINKPSIIIGRNQNTVEEIDTEYVE
CEEECCCCCCCCEEEEEHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHC
KNDVIVVRRLSGGGAVYHDEGNLNFSFITEDDGESFHNFAKFTQPIVEALKRLGVNAELK
CCCEEEEEEECCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
GRNDLLIDGFKVSGNAQFATKGKMFSHGTLMYDLNLDNVAASLKPRKDKIESKGIKSVRS
CCCCEEEEEEEECCCCEEEECCCEEECCEEEEEECHHHHHHCCCCCHHHHHHHHHHHHHH
RVANISDFMDQEMTTEEFRDLLLLYIFDVDKVEDVKEYKLTDADWEKIHEISAKRYGNWD
HHHHHHHHHHHCCCHHHHHHHHHHEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
WNYGKSPKFDLTRTKRFPVGAVDVRLNVQKGIITEIKIFGDFFGVKNVADIEAKLVNTTY
CCCCCCCCCCCEECCCCCCEEEEEEEEECCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHH
KREALAEALSDIEVKEYFGNITKDEFLDLLY
HHHHHHHHHHCCCHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MYFIDNNNEKDPRINLAVEEFILTELELDEPVLLFYINKPSIIIGRNQNTVEEIDTEYVE
CEEECCCCCCCCEEEEEHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHC
KNDVIVVRRLSGGGAVYHDEGNLNFSFITEDDGESFHNFAKFTQPIVEALKRLGVNAELK
CCCEEEEEEECCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
GRNDLLIDGFKVSGNAQFATKGKMFSHGTLMYDLNLDNVAASLKPRKDKIESKGIKSVRS
CCCCEEEEEEEECCCCEEEECCCEEECCEEEEEECHHHHHHCCCCCHHHHHHHHHHHHHH
RVANISDFMDQEMTTEEFRDLLLLYIFDVDKVEDVKEYKLTDADWEKIHEISAKRYGNWD
HHHHHHHHHHHCCCHHHHHHHHHHEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
WNYGKSPKFDLTRTKRFPVGAVDVRLNVQKGIITEIKIFGDFFGVKNVADIEAKLVNTTY
CCCCCCCCCCCEECCCCCCEEEEEEEEECCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHH
KREALAEALSDIEVKEYFGNITKDEFLDLLY
HHHHHHHHHHCCCHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]