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The map label for this gene is gcl [H]

Identifier: 116689899

GI number: 116689899

Start: 2082994

End: 2084769

Strand: Direct

Name: gcl [H]

Synonym: Bcen2424_1878

Alternate gene names: 116689899

Gene position: 2082994-2084769 (Clockwise)

Preceding gene: 116689888

Following gene: 116689900

Centisome position: 59.79

GC content: 67.17

Gene sequence:

>1776_bases
ATGGCCAAGATGAGAGCCGTCGACGCAGCCGTACTCGTGCTCGAGAAGGAAGGCATCCAGACCGCGTTCGGCGTGCCGGG
CGCCGCGATCAATCCGTTCTACTCGGCCATGCGCAAGTCGGGCGGCATCAGCCACGTGCTGGCGCGCCACGTCGAAGGTG
CATCGCACATGGCCGAAGGCTTCACGCGTGCCGCCCCCGGCAACATCGGCGTTTGCATCGGCACGTCGGGCCCCGCCGGC
ACCGACATGATCACCGGCCTCTATTCCGCGTCCGCCGACTCGATTCCGATCCTCGCGATCACCGGCCAGGCACCGCGCGC
GCGTCTCTACAAGGAAGACTTCCAGGCCGTCGACATCGAATCGATCGCGAAGCCGGTCACGAAGTGGGCCGTCACCGTGC
GCGAACCGGCGCTCGTGCCGCGCGTGTTCCAGCAGGCGTTCCACCTGATGCGCTCGGGCCGCCCGGGCCCCGTGCTGGTC
GACCTGCCGATCGACGTGCAGCTCGCCGAAATCGAATTCGACATCGACACGTACGAACCGCTGCCGGTCTACAAGCCCGC
GGCCACCCGCGCGCAGATCGAGAAGGCGCTCGCGATGCTCAACGACGCGGACAAGCCGCTGATCGTGTCGGGCGGCGGCG
TGCTCAACGCGGCGGCCGAAGACCTGCTCGTGCAGTTCGCCGAAACGGTCGGCGTGCCGGTGATCCCGACGCTGATGTCG
TGGGGCGCGATTGCCGACGACCACCCGCTGATGGCCGGCATGGTCGGCTTGCAGACGTCGCACCGCTACGGCAACGCGAC
GCTGCTCGCATCCGACTTCGTGCTCGGCATCGGCAACCGCTGGGCGAACCGCCACACGGGCAGCGTCGAGGTCTATACGA
AGGGCCGCAAGTTCGTGCACGTCGATATCGAGCCGACGCAGATCGGCCGCGTGTTCGGCCCGGACCTCGGCATCGTGTCC
GACGCGAAGGCGGCACTCGAACTGTTCGTCGCGGTCGCGCGGGAATGGAAGGCCGCCGGCAAGCTCAAGGACCGCAGCGC
GTGGGTCGCCGACTGCCAGGCGCGCAAGCGCACGCTGCAGCGCAAGACGCACTTCGACAACGTGCCGGTCAAGCCGCAGC
GCGTGTACGAAGAGATGAACAAGGTGTTCGGCCGCGATACCTGCTACGTCAGCACGATCGGCCTGTCGCAGATCGCCGCC
GCGCAGTTCCTGCACGTGTTCAAGGCACGCAACTGGATCAACTGCGGCCAGGCCGGCCCGCTCGGCTGGACGATCCCCGC
GGCGCTCGGCGTGCGTGCAGCCGACCCGAGCCGCCCGATCGTCGCGCTGTCCGGCGACTACGACTTCCAGTTCATGATCG
AGGAGCTGGCGGCCGGCGCGCAATTCAAGCTGCCGTACGTGCACGTCGTCGTGAACAACTCGTACCTCGGGCTGATCCGC
CAGGCGCAGCGCGCGTTCGACATGGACTACTGCGTGCAGCTCGCGTTCGACAACGTGAACGCGCCGGAACTGAACGGTTA
CGGCGTCGACCACGTGGCCGTCGCGGAAGGCCTCGGCTGCAAGGCGCTGCGCGTGTTCAAGCCGGAAGAGATCGAGCCGG
CGCTGCGCCAGGCGCAAACGCTCGCGGAAGAATTCAGCGTGCCGGTGGTCGTCGAAGTGATCCTCGAGCGCGTGACGAAC
ATCTCGATGGGCACCGAAATCGACGCGATCAACGAGTTCGAGGATCTCGCCGAAAAGGCCGAGCACGCCCCGACCGCGAT
CTCGATGCTCGACTGA

Upstream 100 bases:

>100_bases
GAAATAAGCGACTTCGGCTGATCTTATCAAACCTTTCCTGCATCCCTAAAGTGTCCTCAACAGACCGATTCGCAAGATTC
AACTATCCAAGGAGACATTC

Downstream 100 bases:

>100_bases
ACCGACTGAACCGACTGAACCGACTGAACCGCACGACTTTTTTGACTGACCGACCGAAGAGGCTTAGCTCATGCCGAAGT
TTGCTGCAAACCTGACCATG

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MAKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEGFTRAAPGNIGVCIGTSGPAG
TDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLV
DLPIDVQLAEIEFDIDTYEPLPVYKPAATRAQIEKALAMLNDADKPLIVSGGGVLNAAAEDLLVQFAETVGVPVIPTLMS
WGAIADDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSVEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVS
DAKAALELFVAVAREWKAAGKLKDRSAWVADCQARKRTLQRKTHFDNVPVKPQRVYEEMNKVFGRDTCYVSTIGLSQIAA
AQFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPSRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIR
QAQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVFKPEEIEPALRQAQTLAEEFSVPVVVEVILERVTN
ISMGTEIDAINEFEDLAEKAEHAPTAISMLD

Sequences:

>Translated_591_residues
MAKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEGFTRAAPGNIGVCIGTSGPAG
TDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLV
DLPIDVQLAEIEFDIDTYEPLPVYKPAATRAQIEKALAMLNDADKPLIVSGGGVLNAAAEDLLVQFAETVGVPVIPTLMS
WGAIADDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSVEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVS
DAKAALELFVAVAREWKAAGKLKDRSAWVADCQARKRTLQRKTHFDNVPVKPQRVYEEMNKVFGRDTCYVSTIGLSQIAA
AQFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPSRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIR
QAQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVFKPEEIEPALRQAQTLAEEFSVPVVVEVILERVTN
ISMGTEIDAINEFEDLAEKAEHAPTAISMLD
>Mature_590_residues
AKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEGFTRAAPGNIGVCIGTSGPAGT
DMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLVD
LPIDVQLAEIEFDIDTYEPLPVYKPAATRAQIEKALAMLNDADKPLIVSGGGVLNAAAEDLLVQFAETVGVPVIPTLMSW
GAIADDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSVEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVSD
AKAALELFVAVAREWKAAGKLKDRSAWVADCQARKRTLQRKTHFDNVPVKPQRVYEEMNKVFGRDTCYVSTIGLSQIAAA
QFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPSRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIRQ
AQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVFKPEEIEPALRQAQTLAEEFSVPVVVEVILERVTNI
SMGTEIDAINEFEDLAEKAEHAPTAISMLD

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=570, Percent_Identity=24.0350877192982, Blast_Score=126, Evalue=7e-29,
Organism=Homo sapiens, GI21361361, Length=598, Percent_Identity=23.9130434782609, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1786717, Length=590, Percent_Identity=79.4915254237288, Blast_Score=981, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=544, Percent_Identity=34.0073529411765, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1790104, Length=482, Percent_Identity=33.6099585062241, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1787096, Length=553, Percent_Identity=25.8589511754069, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1788716, Length=477, Percent_Identity=25.5765199161426, Blast_Score=135, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17542570, Length=554, Percent_Identity=25.0902527075812, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17531299, Length=507, Percent_Identity=22.879684418146, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=507, Percent_Identity=22.879684418146, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=598, Percent_Identity=29.7658862876254, Blast_Score=258, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6320816, Length=564, Percent_Identity=24.290780141844, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6323163, Length=496, Percent_Identity=20.9677419354839, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922626, Length=572, Percent_Identity=25.1748251748252, Blast_Score=145, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 63908; Mature: 63777

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS01067 SECE_SEC61G

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEG
CCCCHHHCEEEEEEECCCCCHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
FTRAAPGNIGVCIGTSGPAGTDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIE
HHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLVDLPIDVQLAEIEFDIDTYEP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCC
LPVYKPAATRAQIEKALAMLNDADKPLIVSGGGVLNAAAEDLLVQFAETVGVPVIPTLMS
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
WGAIADDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSVEVYTKGRKFVH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALELFVAVAREWKAAGKLKDRSAWVADCQARKRTLQ
EECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNKVFGRDTCYVSTIGLSQIAAAQFLHVFKARNWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
LGWTIPAALGVRAADPSRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIR
CCEEHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEECCEEEEEECCCHHHHHH
QAQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVFKPEEIEPALRQAQT
HHHHHHCHHHHHHEEECCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCHHHHHHHH
LAEEFSVPVVVEVILERVTNISMGTEIDAINEFEDLAEKAEHAPTAISMLD
HHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure 
AKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEG
CCCHHHCEEEEEEECCCCCHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
FTRAAPGNIGVCIGTSGPAGTDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIE
HHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLVDLPIDVQLAEIEFDIDTYEP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCC
LPVYKPAATRAQIEKALAMLNDADKPLIVSGGGVLNAAAEDLLVQFAETVGVPVIPTLMS
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
WGAIADDHPLMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSVEVYTKGRKFVH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALELFVAVAREWKAAGKLKDRSAWVADCQARKRTLQ
EECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNKVFGRDTCYVSTIGLSQIAAAQFLHVFKARNWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
LGWTIPAALGVRAADPSRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIR
CCEEHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEECCEEEEEECCCHHHHHH
QAQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVFKPEEIEPALRQAQT
HHHHHHCHHHHHHEEECCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCHHHHHHHH
LAEEFSVPVVVEVILERVTNISMGTEIDAINEFEDLAEKAEHAPTAISMLD
HHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]