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The map label for this gene is potF [H]

Identifier: 116689772

GI number: 116689772

Start: 1947272

End: 1948366

Strand: Reverse

Name: potF [H]

Synonym: Bcen2424_1751

Alternate gene names: 116689772

Gene position: 1948366-1947272 (Counterclockwise)

Preceding gene: 116689777

Following gene: 116689771

Centisome position: 55.92

GC content: 63.2

Gene sequence:

>1095_bases
ATGAAAGCAAAGGTAGTGGGCCGGTTCGCAGCGCTCGCGCTGTGCGTGGGTGCGTCGGCGGCAGCGGCGAAGGATACGCA
GCTCAATGTCTATAACTGGTCGGACTACATTGCGAAGGACACGATCCCGAACTTCGAGAAGCAGGCGGGTGTCAAGGTCC
GCTACGACAACTACGACAGCGACGACACGTTGCAGGCGAAACTGCTGACGGGTAGCTCGGGCTACGACATCGTCGTGCCG
ACCAGCAACTACGCGGGCAAGCAGATCGCCGCCGGCATCTTCGCGCCGCTCGACAAGTCGAAGCTGCCGAACCTCAAGTA
CCTCGATCCGCAACTGATGGCGCTCGTCGCCGGCGCGGACCCGGGCAACAAGTACTCGGTGCCCTGGGCATACGGCACGA
CCGGCCTCGCGTACAACCTGACGAAGGCGCAGCAGGCGCTCGGCAAGGTGCCGCTCGACAACTGGGACATCCTGTTCAAG
CCGGAAAACCTCGCGAAGCTGAAGACCTGCGGCGTGTCGGTGCTCGACGCACCCGACCAGATGTTCGCGGCGACGCTGCA
CTACATCGGCAAGGACCCGATGAGCACGAACCCGGCCGACTACAAGGCCGCGATGGACGTGCTGAAGAAGATCCGTCCGT
ACATCACGCAATTCAACTCGTCGGGCTACATCAACGACCTGGTCGGCGGCGACATCTGCTTCGCGTTCGGCTGGTCGGGC
GACGTCGTGATCGCGAAGCATCGCGCGATCGAGGCGAAGAAGCCGTACAAGATCGAGTACTACATTCCGAAGGGCGGTGC
GCCGGTGTGGTTCGACGTGATGGCGATCCCGAAGGATGCGAAGAACAAGGAAGCCGCGCTGCAGTGGATCAACTACATCG
AGGACCCGAAGGTGCACGCGGCGATCACCAACGCGGTGTACTACCCGAGCGCCAACGCCGAGGCACGCAAGTACGTGCGG
CCCGACGTCGCGAACGACCCGGCCGTCTACCCGCCGGCCGACGTCGTGAAGACGCTGTTCCTGCTCAAGCCGCTACCGCC
TGAAATCCAGCGTCTGCAGACGCGTCTGTGGACCGAGCTGAAGTCGGGCCGCTGA

Upstream 100 bases:

>100_bases
TGGTAGTTTTCGGGGTTTTCGAGGGGTGGCGCGCCAGAATGCGCCGCCATGACGAGGCAGACGGCAGCCGGGCAATGCGT
CCGGCCGGAGGGAGAACGGG

Downstream 100 bases:

>100_bases
CGCGAGCCGAAGTGAATCAGCAGTCATGAAGCCCCTGGTGCCCCCGGGGGCTTTGTTCGTCTAACGCAGGAGAGAAGCAG
CACATCATGAATAGCCAGTC

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKAKVVGRFAALALCVGASAAAAKDTQLNVYNWSDYIAKDTIPNFEKQAGVKVRYDNYDSDDTLQAKLLTGSSGYDIVVP
TSNYAGKQIAAGIFAPLDKSKLPNLKYLDPQLMALVAGADPGNKYSVPWAYGTTGLAYNLTKAQQALGKVPLDNWDILFK
PENLAKLKTCGVSVLDAPDQMFAATLHYIGKDPMSTNPADYKAAMDVLKKIRPYITQFNSSGYINDLVGGDICFAFGWSG
DVVIAKHRAIEAKKPYKIEYYIPKGGAPVWFDVMAIPKDAKNKEAALQWINYIEDPKVHAAITNAVYYPSANAEARKYVR
PDVANDPAVYPPADVVKTLFLLKPLPPEIQRLQTRLWTELKSGR

Sequences:

>Translated_364_residues
MKAKVVGRFAALALCVGASAAAAKDTQLNVYNWSDYIAKDTIPNFEKQAGVKVRYDNYDSDDTLQAKLLTGSSGYDIVVP
TSNYAGKQIAAGIFAPLDKSKLPNLKYLDPQLMALVAGADPGNKYSVPWAYGTTGLAYNLTKAQQALGKVPLDNWDILFK
PENLAKLKTCGVSVLDAPDQMFAATLHYIGKDPMSTNPADYKAAMDVLKKIRPYITQFNSSGYINDLVGGDICFAFGWSG
DVVIAKHRAIEAKKPYKIEYYIPKGGAPVWFDVMAIPKDAKNKEAALQWINYIEDPKVHAAITNAVYYPSANAEARKYVR
PDVANDPAVYPPADVVKTLFLLKPLPPEIQRLQTRLWTELKSGR
>Mature_364_residues
MKAKVVGRFAALALCVGASAAAAKDTQLNVYNWSDYIAKDTIPNFEKQAGVKVRYDNYDSDDTLQAKLLTGSSGYDIVVP
TSNYAGKQIAAGIFAPLDKSKLPNLKYLDPQLMALVAGADPGNKYSVPWAYGTTGLAYNLTKAQQALGKVPLDNWDILFK
PENLAKLKTCGVSVLDAPDQMFAATLHYIGKDPMSTNPADYKAAMDVLKKIRPYITQFNSSGYINDLVGGDICFAFGWSG
DVVIAKHRAIEAKKPYKIEYYIPKGGAPVWFDVMAIPKDAKNKEAALQWINYIEDPKVHAAITNAVYYPSANAEARKYVR
PDVANDPAVYPPADVVKTLFLLKPLPPEIQRLQTRLWTELKSGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=360, Percent_Identity=51.1111111111111, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787367, Length=322, Percent_Identity=35.7142857142857, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1787711, Length=319, Percent_Identity=22.884012539185, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39980; Mature: 39980

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKVVGRFAALALCVGASAAAAKDTQLNVYNWSDYIAKDTIPNFEKQAGVKVRYDNYDS
CCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHCCCCCHHHHCCCEEEECCCCC
DDTLQAKLLTGSSGYDIVVPTSNYAGKQIAAGIFAPLDKSKLPNLKYLDPQLMALVAGAD
CCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEECCC
PGNKYSVPWAYGTTGLAYNLTKAQQALGKVPLDNWDILFKPENLAKLKTCGVSVLDAPDQ
CCCCEECCEECCCCCEEEEHHHHHHHHCCCCCCCCEEEECCCCHHHHHHCCEEEECCCHH
MFAATLHYIGKDPMSTNPADYKAAMDVLKKIRPYITQFNSSGYINDLVGGDICFAFGWSG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEECCCC
DVVIAKHRAIEAKKPYKIEYYIPKGGAPVWFDVMAIPKDAKNKEAALQWINYIEDPKVHA
CEEEEECCEECCCCCEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEE
AITNAVYYPSANAEARKYVRPDVANDPAVYPPADVVKTLFLLKPLPPEIQRLQTRLWTEL
EEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KSGR
HCCC
>Mature Secondary Structure
MKAKVVGRFAALALCVGASAAAAKDTQLNVYNWSDYIAKDTIPNFEKQAGVKVRYDNYDS
CCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHCCCCCHHHHCCCEEEECCCCC
DDTLQAKLLTGSSGYDIVVPTSNYAGKQIAAGIFAPLDKSKLPNLKYLDPQLMALVAGAD
CCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEECCC
PGNKYSVPWAYGTTGLAYNLTKAQQALGKVPLDNWDILFKPENLAKLKTCGVSVLDAPDQ
CCCCEECCEECCCCCEEEEHHHHHHHHCCCCCCCCEEEECCCCHHHHHHCCEEEECCCHH
MFAATLHYIGKDPMSTNPADYKAAMDVLKKIRPYITQFNSSGYINDLVGGDICFAFGWSG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEECCCC
DVVIAKHRAIEAKKPYKIEYYIPKGGAPVWFDVMAIPKDAKNKEAALQWINYIEDPKVHA
CEEEEECCEECCCCCEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEE
AITNAVYYPSANAEARKYVRPDVANDPAVYPPADVVKTLFLLKPLPPEIQRLQTRLWTEL
EEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KSGR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]