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The map label for this gene is tuf1

Identifier: 116624204

GI number: 116624204

Start: 6442241

End: 6443428

Strand: Reverse

Name: tuf1

Synonym: Acid_5120

Alternate gene names: 116624204

Gene position: 6443428-6442241 (Counterclockwise)

Preceding gene: 116624205

Following gene: 116624203

Centisome position: 64.66

GC content: 60.27

Gene sequence:

>1188_bases
ATGGCTAAGGAAAAATTCGACCGCAGCAAACCGCACGTAAACATCGGGACGATCGGACACATCGATCACGGCAAGACCAC
CCTGACAGCGGCGATCACTAAAGTGCTGGCCAAGCACAATCCGAAGAACAAATTCCGGAGCTTCGATTCGATCGACAACG
CCCCGGAGGAAAAGGCACGCGGAATCACGATCGCGGTGGCGCACGTCGAGTACGAGACTGCCAAGCGCCATTACGCCCAC
GTGGATTGCCCCGGTCACGCGGATTACATCAAGAACATGATCACCGGCGCGGCGCAGATGGATGGCGCCATCGTGGTGGT
GGCGGCGACCGACGGACCCATGCCGCAAACGCGCGAGCACATCCTGCTCGCCCGCCAGGTCGGCGTACCGTACATCGTAG
TGGCCATGAACAAGGTCGATATGGTGGACGATTCCGAGCTGCTGGACCTGGTGGAGCTGGAAGTCCGCGAGTTGCTGAAG
AGCTATCAATTTCCGGGTGACGATCTCCCCGTGGTTCGCGTGAGCGCGCTGGGCGCGTTGAACGGCGAGCCGCAGTGGGA
AAAGACGGTCGATGAGCTGATGGAAGCGGTAGATAGCTACATTCCGATGCCGGAACGCGCGATCGACAAGCCGTTCATCA
TGCCGATCGAGGACATCTTCTCGATTCAGGGCCGCGGCACGGTGGTGACGGGCCGCATCGAGCGCGGAATCTGCAAGGTT
GGCGAGGAAATGGAAATCGTCGGCTTCCGGGATACGCGCAAGACGGTGGTCACGGGCGTGGAAATGTTCAAGAAGCTGTT
GGACGAAGGGCGCGCCGGGGACAACGTCGGCCTGCTGCTGCGCGGTGTGGAAAAGGACATGGTGGAGCGCGGTCAGGTGA
TCGCGAAGCCGGGTTCGATTACGCCGCATACCAAGTTCAAGGGCGAAGTCTACGTGCTGAGCAAGGAAGAAGGCGGACGT
CATACTCCGTTCTTCAAGGGATACCGGCCGCAGTTCTATTTCCGCACGACGGACGTAACGGGAGTGGCGCAGTTGCCGGA
AGGCACGGAGATGGTGATGCCGGGCGACAACGTGAGCCTCGAAGTGGAACTGATCACTCCCGTGGCCATGGACAAGGGGT
TGCGCTTCGCGATTCGTGAAGGCGGCCGCACGGTTGGCGCGGGTACGGTTACCGAGATTCTTAAGTAG

Upstream 100 bases:

>100_bases
GATGCACTTTGGAAAGTACGAAGAAGTGCCGGCCGCGCAGGCGGAAGAGATTGTCAGCAAGGTCCAGGGCAAGACTACTA
GATAAGGAGGCTTCTTTCTT

Downstream 100 bases:

>100_bases
GCAAACCAATGGCATTGAGAGAACGCATTCGAATCCGTCTGAAGGCATACGATCACCGCATCCTCGATCAGTCCACGACG
GAAATTGTGGATACCGCGAA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAKEKFDRSKPHVNIGTIGHIDHGKTTLTAAITKVLAKHNPKNKFRSFDSIDNAPEEKARGITIAVAHVEYETAKRHYAH
VDCPGHADYIKNMITGAAQMDGAIVVVAATDGPMPQTREHILLARQVGVPYIVVAMNKVDMVDDSELLDLVELEVRELLK
SYQFPGDDLPVVRVSALGALNGEPQWEKTVDELMEAVDSYIPMPERAIDKPFIMPIEDIFSIQGRGTVVTGRIERGICKV
GEEMEIVGFRDTRKTVVTGVEMFKKLLDEGRAGDNVGLLLRGVEKDMVERGQVIAKPGSITPHTKFKGEVYVLSKEEGGR
HTPFFKGYRPQFYFRTTDVTGVAQLPEGTEMVMPGDNVSLEVELITPVAMDKGLRFAIREGGRTVGAGTVTEILK

Sequences:

>Translated_395_residues
MAKEKFDRSKPHVNIGTIGHIDHGKTTLTAAITKVLAKHNPKNKFRSFDSIDNAPEEKARGITIAVAHVEYETAKRHYAH
VDCPGHADYIKNMITGAAQMDGAIVVVAATDGPMPQTREHILLARQVGVPYIVVAMNKVDMVDDSELLDLVELEVRELLK
SYQFPGDDLPVVRVSALGALNGEPQWEKTVDELMEAVDSYIPMPERAIDKPFIMPIEDIFSIQGRGTVVTGRIERGICKV
GEEMEIVGFRDTRKTVVTGVEMFKKLLDEGRAGDNVGLLLRGVEKDMVERGQVIAKPGSITPHTKFKGEVYVLSKEEGGR
HTPFFKGYRPQFYFRTTDVTGVAQLPEGTEMVMPGDNVSLEVELITPVAMDKGLRFAIREGGRTVGAGTVTEILK
>Mature_394_residues
AKEKFDRSKPHVNIGTIGHIDHGKTTLTAAITKVLAKHNPKNKFRSFDSIDNAPEEKARGITIAVAHVEYETAKRHYAHV
DCPGHADYIKNMITGAAQMDGAIVVVAATDGPMPQTREHILLARQVGVPYIVVAMNKVDMVDDSELLDLVELEVRELLKS
YQFPGDDLPVVRVSALGALNGEPQWEKTVDELMEAVDSYIPMPERAIDKPFIMPIEDIFSIQGRGTVVTGRIERGICKVG
EEMEIVGFRDTRKTVVTGVEMFKKLLDEGRAGDNVGLLLRGVEKDMVERGQVIAKPGSITPHTKFKGEVYVLSKEEGGRH
TPFFKGYRPQFYFRTTDVTGVAQLPEGTEMVMPGDNVSLEVELITPVAMDKGLRFAIREGGRTVGAGTVTEILK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=56.2025316455696, Blast_Score=462, Evalue=1e-130,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=31.1363636363636, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=31.1363636363636, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI4503475, Length=444, Percent_Identity=29.2792792792793, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4503471, Length=445, Percent_Identity=29.2134831460674, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI54607086, Length=346, Percent_Identity=28.0346820809249, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI194097354, Length=329, Percent_Identity=28.2674772036474, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI194018522, Length=329, Percent_Identity=28.2674772036474, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI194018520, Length=329, Percent_Identity=28.2674772036474, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI46094014, Length=322, Percent_Identity=28.8819875776398, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI4503507, Length=273, Percent_Identity=27.8388278388278, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI157426893, Length=252, Percent_Identity=25.3968253968254, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=75.3807106598985, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=75.3807106598985, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI2367247, Length=384, Percent_Identity=30.2083333333333, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI48994988, Length=297, Percent_Identity=31.3131313131313, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789108, Length=298, Percent_Identity=29.1946308724832, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=52.5, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=46.1139896373057, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=329, Percent_Identity=34.0425531914894, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=329, Percent_Identity=34.0425531914894, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI115532067, Length=434, Percent_Identity=28.5714285714286, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=434, Percent_Identity=28.5714285714286, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI32566303, Length=313, Percent_Identity=30.9904153354633, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=339, Percent_Identity=29.2035398230088, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=273, Percent_Identity=29.6703296703297, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=173, Percent_Identity=36.4161849710983, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=273, Percent_Identity=28.9377289377289, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=236, Percent_Identity=27.9661016949153, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=64.735516372796, Blast_Score=520, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6325337, Length=443, Percent_Identity=29.7968397291196, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=443, Percent_Identity=29.7968397291196, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=457, Percent_Identity=24.0700218818381, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=340, Percent_Identity=30.2941176470588, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=279, Percent_Identity=28.673835125448, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=376, Percent_Identity=25.7978723404255, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=56.3451776649746, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=56.3451776649746, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI19921738, Length=393, Percent_Identity=49.1094147582697, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI45553807, Length=357, Percent_Identity=32.7731092436975, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI45553816, Length=357, Percent_Identity=32.7731092436975, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24651721, Length=357, Percent_Identity=32.7731092436975, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI17864154, Length=357, Percent_Identity=32.7731092436975, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24652838, Length=450, Percent_Identity=28.8888888888889, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17137572, Length=450, Percent_Identity=28.8888888888889, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45550900, Length=441, Percent_Identity=28.7981859410431, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=30.2631578947368, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17137380, Length=342, Percent_Identity=30.4093567251462, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24647048, Length=268, Percent_Identity=27.9850746268657, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24647046, Length=268, Percent_Identity=27.9850746268657, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28572034, Length=174, Percent_Identity=36.2068965517241, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_SOLUE (Q01SX2)

Other databases:

- EMBL:   CP000473
- EMBL:   CP000473
- RefSeq:   YP_826360.1
- RefSeq:   YP_827533.1
- ProteinModelPortal:   Q01SX2
- SMR:   Q01SX2
- STRING:   Q01SX2
- GeneID:   4425780
- GeneID:   4426624
- GenomeReviews:   CP000473_GR
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_5120
- KEGG:   sus:Acid_6322
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- PhylomeDB:   Q01SX2
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43551; Mature: 43420

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFDRSKPHVNIGTIGHIDHGKTTLTAAITKVLAKHNPKNKFRSFDSIDNAPEEKAR
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHC
GITIAVAHVEYETAKRHYAHVDCPGHADYIKNMITGAAQMDGAIVVVAATDGPMPQTREH
CEEEEEEEEEHHHHHHHEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHH
ILLARQVGVPYIVVAMNKVDMVDDSELLDLVELEVRELLKSYQFPGDDLPVVRVSALGAL
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHCC
NGEPQWEKTVDELMEAVDSYIPMPERAIDKPFIMPIEDIFSIQGRGTVVTGRIERGICKV
CCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEECHHHHHHHHC
GEEMEIVGFRDTRKTVVTGVEMFKKLLDEGRAGDNVGLLLRGVEKDMVERGQVIAKPGSI
CCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCCEEECCCCC
TPHTKFKGEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGVAQLPEGTEMVMPGDNVSL
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCCEE
EVELITPVAMDKGLRFAIREGGRTVGAGTVTEILK
EEEEECCHHHCCCCEEEECCCCCEECCCHHHHHHC
>Mature Secondary Structure 
AKEKFDRSKPHVNIGTIGHIDHGKTTLTAAITKVLAKHNPKNKFRSFDSIDNAPEEKAR
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHC
GITIAVAHVEYETAKRHYAHVDCPGHADYIKNMITGAAQMDGAIVVVAATDGPMPQTREH
CEEEEEEEEEHHHHHHHEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHH
ILLARQVGVPYIVVAMNKVDMVDDSELLDLVELEVRELLKSYQFPGDDLPVVRVSALGAL
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHCC
NGEPQWEKTVDELMEAVDSYIPMPERAIDKPFIMPIEDIFSIQGRGTVVTGRIERGICKV
CCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEECHHHHHHHHC
GEEMEIVGFRDTRKTVVTGVEMFKKLLDEGRAGDNVGLLLRGVEKDMVERGQVIAKPGSI
CCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCCEEECCCCC
TPHTKFKGEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGVAQLPEGTEMVMPGDNVSL
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCCEE
EVELITPVAMDKGLRFAIREGGRTVGAGTVTEILK
EEEEECCHHHCCCCEEEECCCCCEECCCHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA