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The map label for this gene is pit [H]

Identifier: 116622292

GI number: 116622292

Start: 4035202

End: 4036233

Strand: Reverse

Name: pit [H]

Synonym: Acid_3186

Alternate gene names: 116622292

Gene position: 4036233-4035202 (Counterclockwise)

Preceding gene: 116622293

Following gene: 116622290

Centisome position: 40.5

GC content: 60.08

Gene sequence:

>1032_bases
ATGTCCGTTCTTACACTCGCCGCCTTGATCATCGTGATCGCGTTGGTGTTCGATTACATCAATGGCTTTCACGATGCGGC
AAATTCCATCGCCACGATTGTGGCGACCCGGGTACTGACTCCTTTCCAAGCGGTCCTGTGGGCCGCGTTTTTTAATTTTG
CAGCGGCATTCATTTTTGGCACGGCGGTCGCGAAGACCGTGGGCAAAGGCTTTGTAGATCTCGACCTGGTAACACCCTAC
GTAATCATGGCGGGGTTGTTCGGCGCCATCATCTGGGATCTCATCACCTGGTGGCTGGGGCTGCCTACCAGTTCGTCGCA
CGCCCTGATCGGCGGGTACGCGGGAGCGGCCATGGCGCGCGTCGGCCTTCTCCGCGGCTTCCATCACAGCTTCGAGGCCT
TGAATGTCGGCCCCCACGGCGAGTGGCCGTTCACCCTCAAAATGATTATCGGCGCGCCCCTCATCGGCATGGTGTCGGCT
TACGTGCTGATGATTATCGTCTACTGGCTCTTCCGCCACTCCACGCCCTCCAAGATGGACAAGTATTTCCGCAAACTGCA
GTTGCTGTCGGCGGCGGCCTTCAGTCTTTCGCACGGCTCCAACGACGCGCAGAAAACCGCGGGCATTATCACAGGCGTGC
TCTTCACCTCGCGCTATCTGAAAACTTTCGACGTGCCCTCGTGGGTTCTGCTCGCGTCTTATTCGGCGATCGCGCTGGGC
ACCTTGAGCGGCGGATGGCGCATTGTCCGCACCATGGGAGGACGTCTCACCCGCCTCAAACCACGTAGCGGCTTTTGCGC
CGAAACCGGCGCGGCGGCCAGCGTACTGCTTTCCACCTACATGGGCACCCCGGTGTCCACCACTCACGCGATTGCCGGCG
CCATCGCGGGCGTGGGCAGCATCCAGCGCATGAAGGCCGTACGTTGGGGAATCGCCACCAATATCGTGTGGGCGTGGATC
CTGACCATCCCGGCGGCGGCTACGATCGCCTGGCTATCGTACTTTCTTCTGCATTGGACAATCGTGAAGTAG

Upstream 100 bases:

>100_bases
ATCAAGCTGAAGGAAGTCTACGAATTCTTCGAGGCGACCATCGATAGCTGCGAAGACGTGGCCGACGTTCTGCAAAACGT
AGTGGTCAAGAACAGCTAGC

Downstream 100 bases:

>100_bases
CGGCGGCCTCGTCCATCACCCATTCCGCCGCCAGCGCGCTCGCGCCATTAGAGACCGGATCGATGCCGCGCAGCACCGAC
GTGACAAAATCTTCCACGAA

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSVLTLAALIIVIALVFDYINGFHDAANSIATIVATRVLTPFQAVLWAAFFNFAAAFIFGTAVAKTVGKGFVDLDLVTPY
VIMAGLFGAIIWDLITWWLGLPTSSSHALIGGYAGAAMARVGLLRGFHHSFEALNVGPHGEWPFTLKMIIGAPLIGMVSA
YVLMIIVYWLFRHSTPSKMDKYFRKLQLLSAAAFSLSHGSNDAQKTAGIITGVLFTSRYLKTFDVPSWVLLASYSAIALG
TLSGGWRIVRTMGGRLTRLKPRSGFCAETGAAASVLLSTYMGTPVSTTHAIAGAIAGVGSIQRMKAVRWGIATNIVWAWI
LTIPAAATIAWLSYFLLHWTIVK

Sequences:

>Translated_343_residues
MSVLTLAALIIVIALVFDYINGFHDAANSIATIVATRVLTPFQAVLWAAFFNFAAAFIFGTAVAKTVGKGFVDLDLVTPY
VIMAGLFGAIIWDLITWWLGLPTSSSHALIGGYAGAAMARVGLLRGFHHSFEALNVGPHGEWPFTLKMIIGAPLIGMVSA
YVLMIIVYWLFRHSTPSKMDKYFRKLQLLSAAAFSLSHGSNDAQKTAGIITGVLFTSRYLKTFDVPSWVLLASYSAIALG
TLSGGWRIVRTMGGRLTRLKPRSGFCAETGAAASVLLSTYMGTPVSTTHAIAGAIAGVGSIQRMKAVRWGIATNIVWAWI
LTIPAAATIAWLSYFLLHWTIVK
>Mature_342_residues
SVLTLAALIIVIALVFDYINGFHDAANSIATIVATRVLTPFQAVLWAAFFNFAAAFIFGTAVAKTVGKGFVDLDLVTPYV
IMAGLFGAIIWDLITWWLGLPTSSSHALIGGYAGAAMARVGLLRGFHHSFEALNVGPHGEWPFTLKMIIGAPLIGMVSAY
VLMIIVYWLFRHSTPSKMDKYFRKLQLLSAAAFSLSHGSNDAQKTAGIITGVLFTSRYLKTFDVPSWVLLASYSAIALGT
LSGGWRIVRTMGGRLTRLKPRSGFCAETGAAASVLLSTYMGTPVSTTHAIAGAIAGVGSIQRMKAVRWGIATNIVWAWIL
TIPAAATIAWLSYFLLHWTIVK

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=164, Percent_Identity=34.7560975609756, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI5803173, Length=165, Percent_Identity=34.5454545454545, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1789360, Length=226, Percent_Identity=33.1858407079646, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789907, Length=226, Percent_Identity=33.1858407079646, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32566716, Length=179, Percent_Identity=33.5195530726257, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17539280, Length=173, Percent_Identity=32.3699421965318, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71981576, Length=169, Percent_Identity=31.9526627218935, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25146401, Length=178, Percent_Identity=30.3370786516854, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17536725, Length=179, Percent_Identity=27.9329608938547, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17557328, Length=152, Percent_Identity=30.2631578947368, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319773, Length=158, Percent_Identity=29.746835443038, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356511, Length=186, Percent_Identity=29.0322580645161, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 36950; Mature: 36819

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVLTLAALIIVIALVFDYINGFHDAANSIATIVATRVLTPFQAVLWAAFFNFAAAFIFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]